Restriction map for
pFN21AB9735
(Circular) (Six-base) MAPSORT of: pFN21AB9735.seq Check: 8200 from: 1 to: 6154
pFN21AB9735 6154 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3964 278
Size: 53 83 186 936 2428 2468
Fragments arranged by size:
2468 2428 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2346 3497 1937
Size: 409 1151 4594
Fragments arranged by size:
4594 1151 409
Acc65I G'GTAC_C
Cuts at: 3475 3475
Size: 6154
AclI AA'CG_TT
Cuts at: 1563 4281 4654 1563
Size: 2718 373 3063
Fragments arranged by size:
3063 2718 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2198 2417 3363 4217 5601 5869 1632
Size: 566 219 946 854 1384 268 1917
Fragments arranged by size:
1917 1384 946 854 566 268 219
AfeI AGC'GCT
Cuts at: 3014 3014
Size: 6154
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5957
AflIII A'CryG_T
Cuts at: 1203 2289 5053 6030 1203
Size: 1086 2764 977 1327
Fragments arranged by size:
2764 1327 1086 977
AgeI A'CCGG_T
Cuts at: 4985 4985
Size: 6154
AhdI GACnn_n'nnGTC
Cuts at: 4883 4883
Size: 6154
AleI CACnn'nnGTG
Cuts at: 1166 2858 1166
Size: 1692 4462
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2922 1184
Size: 1738 4416
AlwNI CAG_nnn'CTG
Cuts at: 3178 3206 3281 3897 5469 3178
Size: 28 75 616 1572 3863
Fragments arranged by size:
3863 1572 616 75 28
ApaI G_GGCC'C
Cuts at: 2283 2283
Size: 6154
ApaLI G'TGCA_C
Cuts at: 4211 5367 4211
Size: 1156 4998
ApoI r'AATT_y
Cuts at: 1493 2111 2234 3463 3689 3841 5950 1493
Size: 618 123 1229 226 152 2109 1697
Fragments arranged by size:
2109 1697 1229 618 226 152 123
AseI AT'TA_AT
Cuts at: 160 1032 4708 160
Size: 872 3676 1606
Fragments arranged by size:
3676 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6154
AvaI C'yCGr_G
Cuts at: 1870 1943 2038 3479 1870
Size: 73 95 1441 4545
Fragments arranged by size:
4545 1441 95 73
BamHI G'GATC_C
Cuts at: 3484 3851 3484
Size: 367 5787
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2686 3475 4930 618
Size: 325 217 627 899 789 1455 1842
Fragments arranged by size:
1842 1455 899 789 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2283 3473 1391
Size: 892 1190 4072
Fragments arranged by size:
4072 1190 892
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5183
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4345 4379 1903
Size: 34 2408 34 3678
Fragments arranged by size:
3678 2408 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2714 4046 5256 2714
Size: 1332 1210 3612
Fragments arranged by size:
3612 1332 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6154
BfrBI ATG'CAT
Cuts at: 2596 2596
Size: 6154
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4765 136
Size: 107 122 71 631 605 3093 1525
Fragments arranged by size:
3093 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6149 6149
Size: 6154
BlpI GC'TnA_GC
Cuts at: 2943 3298 3571 2943
Size: 355 273 5526
Fragments arranged by size:
5526 355 273
Bme1580I G_kGCm'C
Cuts at: 1790 2283 2691 4215 5371 1790
Size: 493 408 1524 1156 2573
Fragments arranged by size:
2573 1524 1156 493 408
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6154
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2970 3157 3194
Size: 416 53 83 399 24 1553 187 37
Cuts at: 3194 3433 3885 4843 442
Size: 239 452 958 1753
Fragments arranged by size:
1753 1553 958 452 416 399 239 187
83 53 37 24
BmtI G_CTAG'C
Cuts at: 1056 2220 1056
Size: 1164 4990
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2365 2365
Size: 6154
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2622 3041 3258 4814 1239
Size: 1383 419 217 1556 2579
Fragments arranged by size:
2579 1556 1383 419 217
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3210 4279 5144 5442 3210
Size: 1069 865 298 3922
Fragments arranged by size:
3922 1069 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2571 2938 4817 882
Size: 617 1072 367 1879 2219
Fragments arranged by size:
2219 1879 1072 617 367
BsaAI yAC'GTr
Cuts at: 493 1204 3945 493
Size: 711 2741 2702
Fragments arranged by size:
2741 2702 711
BsaBI GATnn'nnATC
Cuts at: 2891 2991 6148 2891
Size: 100 3157 2897
Fragments arranged by size:
3157 2897 100
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3961 4343 4978 275
Size: 53 83 186 936 2428 382 635 1451
Fragments arranged by size:
2428 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4587 4985 5259 5406 5817 4587
Size: 398 274 147 411 4924
Fragments arranged by size:
4924 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2146 2176 2524 2554 2792
Size: 30 165 30 743 30 348 30 238
Cuts at: 2792 2822 3256 3286 1178
Size: 30 434 30 4046
Fragments arranged by size:
4046 743 434 348 238 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2204 2800 1186
Size: 1018 596 4540
Fragments arranged by size:
4540 1018 596
BseYI C'CCAG_C
Cuts at: 2119 2276 2922 5357 5853 2119
Size: 157 646 2435 496 2420
Fragments arranged by size:
2435 2420 646 496 157
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3192 1163
Size: 573 1456 4125
Fragments arranged by size:
4125 1456 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3604 4365 5393 1540
Size: 275 186 1603 761 1028 2301
Fragments arranged by size:
2301 1603 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4215 4300 5371 4215
Size: 85 1071 4998
Fragments arranged by size:
4998 1071 85
BsmI GAATG_Cn'
Cuts at: 3665 3758 3665
Size: 93 6061
BsmBI CGTCTCn'nnnn_
Cuts at: 2004 2004
Size: 6154
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2283 2691 3473 4215 4300 5371 1391
Size: 399 493 408 782 742 85 1071 2174
Fragments arranged by size:
2174 1071 782 742 493 408 399 85
BspEI T'CCGG_A
Cuts at: 5817 5817
Size: 6154
BspHI T'CATG_A
Cuts at: 3423 4043 5773 3423
Size: 620 1730 3804
Fragments arranged by size:
3804 1730 620
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3509 844
Size: 2665 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4041 1394
Size: 429 2218 3507
Fragments arranged by size:
3507 2218 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2038 2142 3157 3322 4649 4823 65
Size: 1183 790 104 1015 165 1327 174 1396
Fragments arranged by size:
1396 1327 1183 1015 790 174 165 104
BsrFI r'CCGG_y
Cuts at: 1700 1810 4798 4985 1700
Size: 110 2988 187 2869
Fragments arranged by size:
2988 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6154
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6154
BssSI C'ACGA_G
Cuts at: 4214 5226 4214
Size: 1012 5142
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6154
BstBI TT'CG_AA
Cuts at: 4958 4958
Size: 6154
BstEII G'GTnAC_C
Cuts at: 1184 2410 1184
Size: 1226 4928
BstXI CCAn_nnnn'nTGG
Cuts at: 2857 2857
Size: 6154
BstYI r'GATC_y
Cuts at: 1972 2992 3484 3851 4236 4253 5705 5791 5803
Size: 1020 492 367 385 17 1452 86 12
Cuts at: 5803 6149 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1020 492 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2682 2856 513
Size: 552 939 678 174 3811
Fragments arranged by size:
3811 939 678 552 174
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5415
Fragments arranged by size:
5415 683 56
BtsI GCAGTG_nn'
Cuts at: 3204 3670 4464 4484 3204
Size: 466 794 20 4874
Fragments arranged by size:
4874 794 466 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6154
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6119
DraI TTT'AAA
Cuts at: 3458 3458
Size: 6154
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3200 3433 5161 809
Size: 507 1884 233 1728 1802
Fragments arranged by size:
1884 1802 1728 507 233
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2667 2749 3351
Size: 54 1425 50 275 18 837 82 602
Cuts at: 3351 3375 3601 4490 6119 8
Size: 24 226 889 1629 43
Fragments arranged by size:
1629 1425 889 837 602 275 226 82
54 50 43 24 18
EagI C'GGCC_G
Cuts at: 1537 1812 3601 1537
Size: 275 1789 4090
Fragments arranged by size:
4090 1789 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2207 2325 4084 1353
Size: 854 118 1759 3423
Fragments arranged by size:
3423 1759 854 118
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4741 5115 5261 387
Size: 1064 138 3152 374 146 1280
Fragments arranged by size:
3152 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2198 2417 2622 3041 3258 3363 4217
Size: 393 566 219 205 419 217 105 854
Cuts at: 4217 4814 5601 5869 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 854 787 597 566 419 393 268
219 217 205 105
EcoO109I rG'GnC_Cy
Cuts at: 2280 2633 2280
Size: 353 5801
EcoRI G'AATT_C
Cuts at: 2234 3463 3841 2234
Size: 1229 378 4547
Fragments arranged by size:
4547 1229 378
FspI TGC'GCA
Cuts at: 4660 4660
Size: 6154
HaeII r_GCGC'y
Cuts at: 1678 3016 5301 1678
Size: 1338 2285 2531
Fragments arranged by size:
2531 2285 1338
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2897 2929 4795 4827 4869
Size: 32 135 32 1325 32 1866 32 42
Cuts at: 4869 4901 1373
Size: 32 2626
Fragments arranged by size:
2626 1866 1325 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2722 3498 3744 669
Size: 988 281 784 776 246 3079
Fragments arranged by size:
3079 988 784 776 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6154
HpaI GTT'AAC
Cuts at: 2722 3744 2722
Size: 1022 5132
KpnI G_GTAC'C
Cuts at: 3479 3479
Size: 6154
MfeI C'AATT_G
Cuts at: 3753 3753
Size: 6154
MluI A'CGCG_T
Cuts at: 6030 6030
Size: 6154
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2189 2528 2574 5268 5452 1305
Size: 884 339 46 2694 184 2007
Fragments arranged by size:
2694 2007 884 339 184 46
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2669 2751 3353 3377 10
Size: 54 1425 343 837 82 602 24 2787
Fragments arranged by size:
2787 1425 837 602 343 82 54 24
MslI CAynn'nnrTG
Cuts at: 518 1166 2094 2621 2858 2904 3371 4112 4471
Size: 648 928 527 237 46 467 741 359
Cuts at: 4471 4630 518
Size: 159 2042
Fragments arranged by size:
2042 928 741 648 527 467 359 237
159 46
MspA1I CmG'CkG
Cuts at: 1606 3048 3124 3341 3570 4247 5395 5640 1606
Size: 1442 76 217 229 677 1148 245 2120
Fragments arranged by size:
2120 1442 1148 677 245 229 217 76
NcoI C'CATG_G
Cuts at: 513 1065 2004 2682 2856 513
Size: 552 939 678 174 3811
Fragments arranged by size:
3811 939 678 552 174
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6154
NheI G'CTAG_C
Cuts at: 1052 2216 1052
Size: 1164 4990
NotI GC'GGCC_GC
Cuts at: 3601 3601
Size: 6154
NsiI A_TGCA'T
Cuts at: 2598 2598
Size: 6154
NspI r_CATG'y
Cuts at: 1132 1229 2293 3403 3419 3512 5057 1132
Size: 97 1064 1110 16 93 1545 2229
Fragments arranged by size:
2229 1545 1110 1064 97 93 16
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6154
PciI A'CATG_T
Cuts at: 2289 5053 2289
Size: 2764 3390
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6154
PmeI GTTT'AAAC
Cuts at: 3458 3458
Size: 6154
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4223 4255 5736 5768 4223
Size: 32 1481 32 4609
Fragments arranged by size:
4609 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2677 1613
Size: 1064 5090
PsiI TTA'TAA
Cuts at: 3724 3724
Size: 6154
PspOMI G'GGCC_C
Cuts at: 2279 2279
Size: 6154
PstI C_TGCA'G
Cuts at: 830 2251 3040 3340 3506 830
Size: 1421 789 300 166 3478
Fragments arranged by size:
3478 1421 789 300 166
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6154
PvuII CAG'CTG
Cuts at: 3124 3341 3124
Size: 217 5937
SalI G'TCGA_C
Cuts at: 1936 3496 1936
Size: 1560 4594
SapI GCTCTTCn'nnn_
Cuts at: 2325 2325
Size: 6154
SbfI CC_TGCA'GG
Cuts at: 3506 3506
Size: 6154
ScaI AGT'ACT
Cuts at: 2959 4402 6114 2959
Size: 1443 1712 2999
Fragments arranged by size:
2999 1712 1443
SfcI C'TryA_G
Cuts at: 826 1045 2247 3036 3212 3336 3502 4637 5318
Size: 219 1202 789 176 124 166 1135 681
Cuts at: 5318 5509 826
Size: 191 1471
Fragments arranged by size:
1471 1202 1135 789 681 219 191 176
166 124
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6154
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6154
SmaI CCC'GGG
Cuts at: 1872 2040 3481 1872
Size: 168 1441 4545
Fragments arranged by size:
4545 1441 168
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3225 4258 5159 5421 820
Size: 197 926 1282 1033 901 262 1553
Fragments arranged by size:
1553 1282 1033 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3945 493
Size: 3452 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6154
SphI G_CATG'C
Cuts at: 1132 3403 3512 1132
Size: 2271 109 3774
Fragments arranged by size:
3774 2271 109
SspI AAT'ATT
Cuts at: 5 52 4078 5
Size: 47 4026 2081
Fragments arranged by size:
4026 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6154
StyI C'CwwG_G
Cuts at: 513 1065 2004 2682 2856 3246 3593 513
Size: 552 939 678 174 390 347 3074
Fragments arranged by size:
3074 939 678 552 390 347 174
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2461 3061 3349 4181 4198 4351 4536
Size: 1255 881 600 288 832 17 153 185
Cuts at: 4536 4981 325
Size: 445 1498
Fragments arranged by size:
1498 1255 881 832 600 445 288 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2641 2957 4400
Size: 274 80 33 51 1443 664 316 1443
Cuts at: 4400 6112 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1443 664 316 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2524 2556 2792 2823 2824 2855 6114 6146 2524
Size: 32 236 31 1 31 3259 32 2532
Fragments arranged by size:
3259 2532 236 32 32 31 31 1
Tth111I GACn'n_nGTC
Cuts at: 1316 2135 1316
Size: 819 5335
XbaI T'CTAG_A
Cuts at: 3490 3490
Size: 6154
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2557 2557
Size: 6154
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6154
XmaI C'CCGG_G
Cuts at: 1870 2038 3479 1870
Size: 168 1441 4545
Fragments arranged by size:
4545 1441 168
XmnI GAAnn'nnTTC
Cuts at: 4283 4283
Size: 6154
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3962 276
Size: 53 83 186 936 2428 2468
Fragments arranged by size:
2468 2428 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BbvCI Bpu10I
BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PfoI PmlI PpuMI PspXI PsrI RsrII SacI SacII
SanDI SexAI SfiI SfoI SrfI SwaI