Restriction map for
pFN21AB9731
(Circular) (Six-base) MAPSORT of: pFN21AB9731.seq Check: 6479 from: 1 to: 6127
pFN21AB9731 6127 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2089 2694 2941 2089
Size: 605 247 5275
Fragments arranged by size:
5275 605 247
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3937 278
Size: 53 83 186 936 2401 2468
Fragments arranged by size:
2468 2401 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2028 3470 1937
Size: 91 1442 4594
Fragments arranged by size:
4594 1442 91
Acc65I G'GTAC_C
Cuts at: 3448 3448
Size: 6127
AclI AA'CG_TT
Cuts at: 1563 4254 4627 1563
Size: 2691 373 3063
Fragments arranged by size:
3063 2691 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4190 5574 5842 1632
Size: 2558 1384 268 1917
Fragments arranged by size:
2558 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5930
AflIII A'CryG_T
Cuts at: 1203 2121 2280 5026 6003 1203
Size: 918 159 2746 977 1327
Fragments arranged by size:
2746 1327 977 918 159
AgeI A'CCGG_T
Cuts at: 4958 4958
Size: 6127
AhdI GACnn_n'nnGTC
Cuts at: 4856 4856
Size: 6127
AleI CACnn'nnGTG
Cuts at: 1166 2220 1166
Size: 1054 5073
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6127
AlwNI CAG_nnn'CTG
Cuts at: 2590 2839 3332 3870 5442 2590
Size: 249 493 538 1572 3275
Fragments arranged by size:
3275 1572 538 493 249
ApaLI G'TGCA_C
Cuts at: 4184 5340 4184
Size: 1156 4971
ApoI r'AATT_y
Cuts at: 1493 2084 3436 3662 3814 5923 1493
Size: 591 1352 226 152 2109 1697
Fragments arranged by size:
2109 1697 1352 591 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4681 160
Size: 872 3649 1606
Fragments arranged by size:
3649 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6127
AvaI C'yCGr_G
Cuts at: 1870 1943 2227 3452 1870
Size: 73 284 1225 4545
Fragments arranged by size:
4545 1225 284 73
AvrII C'CTAG_G
Cuts at: 2737 2737
Size: 6127
BamHI G'GATC_C
Cuts at: 3457 3824 3457
Size: 367 5760
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2138 2222 3448 4903 618
Size: 325 217 627 351 84 1226 1455 1842
Fragments arranged by size:
1842 1455 1226 627 351 325 217 84
BanII G_rGCy'C
Cuts at: 1391 2637 2671 3339 3446 1391
Size: 1246 34 668 107 4072
Fragments arranged by size:
4072 1246 668 107 34
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5156
BbvCI CC'TCA_GC
Cuts at: 3239 3239
Size: 6127
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2033 2067 4318 4352 1903
Size: 34 50 34 12 34 2251 34 3678
Fragments arranged by size:
3678 2251 50 34 34 34 34 12
BciVI GTATCCnnnnn_n'
Cuts at: 4019 5229 4019
Size: 1210 4917
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6127
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4738 136
Size: 107 122 71 631 605 3066 1525
Fragments arranged by size:
3066 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6122 6122
Size: 6127
BlpI GC'TnA_GC
Cuts at: 2909 3544 2909
Size: 635 5492
Bme1580I G_kGCm'C
Cuts at: 1790 4188 5344 1790
Size: 2398 1156 2573
Fragments arranged by size:
2573 2398 1156
BmgBI CAC'GTC
Cuts at: 1318 2124 2728 1318
Size: 806 604 4717
Fragments arranged by size:
4717 806 604
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3858 4816 442
Size: 416 53 83 399 24 2441 958 1753
Fragments arranged by size:
2441 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6127
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4787 1239
Size: 3548 2579
Bpu10I CC'TnA_GC
Cuts at: 3239 3325 3239
Size: 86 6041
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2339 4252 5117 5415 2339
Size: 1913 865 298 3051
Fragments arranged by size:
3051 1913 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3290 4790 882
Size: 617 1791 1500 2219
Fragments arranged by size:
2219 1791 1500 617
BsaAI yAC'GTr
Cuts at: 493 1204 3918 493
Size: 711 2714 2702
Fragments arranged by size:
2714 2702 711
BsaBI GATnn'nnATC
Cuts at: 6121 6121
Size: 6127
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3934 4316 4951 275
Size: 53 83 186 936 2401 382 635 1451
Fragments arranged by size:
2401 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2256 2409 4560 4958 5232 5379 5790 2256
Size: 153 2151 398 274 147 411 2593
Fragments arranged by size:
2593 2151 411 398 274 153 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2270 2300 2471 2501 1178
Size: 30 165 30 867 30 171 30 4804
Fragments arranged by size:
4804 867 171 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2275 1186
Size: 1089 5038
BseYI C'CCAG_C
Cuts at: 2065 2620 3127 3140 3396 5330 5826 2065
Size: 555 507 13 256 1934 496 2366
Fragments arranged by size:
2366 1934 555 507 496 256 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2040 2117 2969 3155 1163
Size: 573 304 77 852 186 4135
Fragments arranged by size:
4135 852 573 304 186 77
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2980 3577 4338 5366 1540
Size: 275 186 979 597 761 1028 2301
Fragments arranged by size:
2301 1028 979 761 597 275 186
BsiHKAI G_wGCw'C
Cuts at: 2637 2685 4188 4273 5344 2637
Size: 48 1503 85 1071 3420
Fragments arranged by size:
3420 1503 1071 85 48
BsmI GAATG_Cn'
Cuts at: 3638 3731 3638
Size: 93 6034
BsmBI CGTCTCn'nnnn_
Cuts at: 2515 2515
Size: 6127
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2637 2671 2685 3339 3446 4188 4273
Size: 399 847 34 14 654 107 742 85
Cuts at: 4273 5344 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 847 742 654 399 107 85
34 14
BspEI T'CCGG_A
Cuts at: 2256 5790 2256
Size: 3534 2593
BspHI T'CATG_A
Cuts at: 4016 5746 4016
Size: 1730 4397
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2089 2694 2941 3482 844
Size: 1245 605 247 541 3489
Fragments arranged by size:
3489 1245 605 541 247
BsrBI CCG'CTC
Cuts at: 1394 1823 2404 4014 1394
Size: 429 581 1610 3507
Fragments arranged by size:
3507 1610 581 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2122 2749 4622 4796 65
Size: 1183 874 627 1873 174 1396
Fragments arranged by size:
1873 1396 1183 874 627 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2301 4771 4958 1700
Size: 110 491 2470 187 2869
Fragments arranged by size:
2869 2470 491 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6127
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6127
BssSI C'ACGA_G
Cuts at: 4187 5199 4187
Size: 1012 5115
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2689 3121 3155 794
Size: 1895 432 34 3766
Fragments arranged by size:
3766 1895 432 34
BstBI TT'CG_AA
Cuts at: 4931 4931
Size: 6127
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6127
BstXI CCAn_nnnn'nTGG
Cuts at: 2164 2164
Size: 6127
BstYI r'GATC_y
Cuts at: 1972 2708 3066 3457 3824 4209 4226 5678 5764
Size: 736 358 391 367 385 17 1452 86
Cuts at: 5764 5776 6122 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 736 391 385 367 358 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2205 2712 513
Size: 552 1140 507 3928
Fragments arranged by size:
3928 1140 552 507
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2539 3159 487
Size: 683 56 1313 620 3455
Fragments arranged by size:
3455 1313 683 620 56
BtsI GCAGTG_nn'
Cuts at: 2312 2764 3643 4437 4457 2312
Size: 452 879 794 20 3982
Fragments arranged by size:
3982 879 794 452 20
ClaI AT'CG_AT
Cuts at: 1554 2847 1554
Size: 1293 4834
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6092
DraI TTT'AAA
Cuts at: 3431 3431
Size: 6127
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2753 5134 809
Size: 507 1437 2381 1802
Fragments arranged by size:
2381 1802 1437 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2040 2998 3093
Size: 54 1425 50 275 18 210 958 95
Cuts at: 3093 3574 4463 6092 8
Size: 481 889 1629 43
Fragments arranged by size:
1629 1425 958 889 481 275 210 95
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3574 1537
Size: 275 1762 4090
Fragments arranged by size:
4090 1762 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3089 4057 1353
Size: 1736 968 3423
Fragments arranged by size:
3423 1736 968
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2492 2795 4714 5088 5234 387
Size: 1064 138 903 303 1919 374 146 1280
Fragments arranged by size:
1919 1280 1064 903 374 303 146 138
EcoICRI GAG'CTC
Cuts at: 2635 2635
Size: 6127
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 4190 4787 5574 5842 1239
Size: 393 2558 597 787 268 1524
Fragments arranged by size:
2558 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 3113 3185 3113
Size: 72 6055
EcoO109I rG'GnC_Cy
Cuts at: 2482 3135 2482
Size: 653 5474
EcoRI G'AATT_C
Cuts at: 3436 3814 3436
Size: 378 5749
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2269 2380 2269
Size: 111 6016
FspI TGC'GCA
Cuts at: 4633 4633
Size: 6127
HaeII r_GCGC'y
Cuts at: 1678 2178 5274 1678
Size: 500 3096 2531
Fragments arranged by size:
3096 2531 500
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2404 2436 2471 2503 4768
Size: 32 135 32 832 32 35 32 2265
Cuts at: 4768 4800 4842 4874 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2265 832 135 42 35 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2029 2106 3471 3717 669
Size: 988 281 91 77 1365 246 3079
Fragments arranged by size:
3079 1365 988 281 246 91 77
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6127
HpaI GTT'AAC
Cuts at: 3717 3717
Size: 6127
KpnI G_GTAC'C
Cuts at: 3452 3452
Size: 6127
MfeI C'AATT_G
Cuts at: 3726 3726
Size: 6127
MluI A'CGCG_T
Cuts at: 6003 6003
Size: 6127
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5241 5425 1305
Size: 3936 184 2007
Fragments arranged by size:
3936 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3095 10
Size: 54 1425 343 1263 3042
Fragments arranged by size:
3042 1425 1263 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2036 2220 4085 4444 4603 518
Size: 648 870 184 1865 359 159 2042
Fragments arranged by size:
2042 1865 870 648 359 184 159
MspA1I CmG'CkG
Cuts at: 1606 2587 3543 4220 5368 5613 1606
Size: 981 956 677 1148 245 2120
Fragments arranged by size:
2120 1148 981 956 677 245
NaeI GCC'GGC
Cuts at: 2303 2303
Size: 6127
NcoI C'CATG_G
Cuts at: 513 1065 2712 513
Size: 552 1647 3928
Fragments arranged by size:
3928 1647 552
NdeI CA'TA_TG
Cuts at: 387 3195 387
Size: 2808 3319
NgoMIV G'CCGG_C
Cuts at: 2301 2301
Size: 6127
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6127
NotI GC'GGCC_GC
Cuts at: 3574 3574
Size: 6127
NspI r_CATG'y
Cuts at: 1132 1229 2284 3485 5030 1132
Size: 97 1055 1201 1545 2229
Fragments arranged by size:
2229 1545 1201 1055 97
PasI CC'CwG_GG
Cuts at: 1348 3273 1348
Size: 1925 4202
PciI A'CATG_T
Cuts at: 2280 5026 2280
Size: 2746 3381
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6127
PfoI T'CCnGG_A
Cuts at: 2513 2832 2513
Size: 319 5808
PmeI GTTT'AAAC
Cuts at: 3431 3431
Size: 6127
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4196 4228 5709 5741 4196
Size: 32 1481 32 4582
Fragments arranged by size:
4582 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2482 3135 2482
Size: 653 5474
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6127
PsiI TTA'TAA
Cuts at: 3697 3697
Size: 6127
PspXI vC'TCGA_Gb
Cuts at: 2227 2227
Size: 6127
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2267 2299 2267
Size: 32 6095
PstI C_TGCA'G
Cuts at: 830 2294 2351 2475 2844 3479 830
Size: 1464 57 124 369 635 3478
Fragments arranged by size:
3478 1464 635 369 124 57
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6127
PvuII CAG'CTG
Cuts at: 2587 2587
Size: 6127
SacI G_AGCT'C
Cuts at: 2637 2637
Size: 6127
SalI G'TCGA_C
Cuts at: 1936 2027 3469 1936
Size: 91 1442 4594
Fragments arranged by size:
4594 1442 91
SanDI GG'GwC_CC
Cuts at: 3135 3135
Size: 6127
SapI GCTCTTCn'nnn_
Cuts at: 3089 3089
Size: 6127
SbfI CC_TGCA'GG
Cuts at: 2475 3479 2475
Size: 1004 5123
ScaI AGT'ACT
Cuts at: 4375 6087 4375
Size: 1712 4415
SexAI A'CCwGG_T
Cuts at: 2649 2649
Size: 6127
SfcI C'TryA_G
Cuts at: 826 1045 2047 2290 2347 2471 2840 3475 4610
Size: 219 1002 243 57 124 369 635 1135
Cuts at: 4610 5291 5482 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1002 681 635 369 243 219
191 124 57
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6127
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6127
SmaI CCC'GGG
Cuts at: 1872 3454 1872
Size: 1582 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2227 2354 4231 5132 5394 820
Size: 197 926 284 127 1877 901 262 1553
Fragments arranged by size:
1877 1553 926 901 284 262 197 127
SnaBI TAC'GTA
Cuts at: 493 3918 493
Size: 3425 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6127
SphI G_CATG'C
Cuts at: 1132 3485 1132
Size: 2353 3774
SspI AAT'ATT
Cuts at: 5 52 4051 5
Size: 47 3999 2081
Fragments arranged by size:
3999 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6127
StyI C'CwwG_G
Cuts at: 513 1065 2712 2737 2901 3566 513
Size: 552 1647 25 164 665 3074
Fragments arranged by size:
3074 1647 665 552 164 25
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2156 4154 4171 4324 4509 4954 325
Size: 1255 576 1998 17 153 185 445 1498
Fragments arranged by size:
1998 1498 1255 576 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2283 2613 4373
Size: 274 80 33 51 1443 306 330 1760
Cuts at: 4373 6085 96
Size: 1712 138
Fragments arranged by size:
1760 1712 1443 330 306 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2270 2302 6087 6119 2270
Size: 32 3785 32 2278
Fragments arranged by size:
3785 2278 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6127
XbaI T'CTAG_A
Cuts at: 3463 3463
Size: 6127
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2710 2710
Size: 6127
XhoI C'TCGA_G
Cuts at: 1943 2227 1943
Size: 284 5843
XmaI C'CCGG_G
Cuts at: 1870 3452 1870
Size: 1582 4545
XmnI GAAnn'nnTTC
Cuts at: 2363 4256 2363
Size: 1893 4234
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3935 276
Size: 53 83 186 936 2401 2468
Fragments arranged by size:
2468 2401 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI
PsrI PstI PvuI PvuII SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI BaeI BbeI BfrBI BplI
BsiWI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI
NarI NruI NsiI PacI PmlI PspOMI RsrII SacII
SfiI SfoI SrfI SwaI