Restriction map for
pFN21AB9729
(Circular) (Six-base) MAPSORT of: pFN21AB9729.seq Check: 7086 from: 1 to: 6043
pFN21AB9729 6043 bp
With 182 enzymes: SgfI *
October 20, 2009 10:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3329 3329
Size: 6043
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3853 278
Size: 53 83 186 936 2317 2468
Fragments arranged by size:
2468 2317 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2167 3386 1937
Size: 230 1219 4594
Fragments arranged by size:
4594 1219 230
Acc65I G'GTAC_C
Cuts at: 3364 3364
Size: 6043
AclI AA'CG_TT
Cuts at: 1563 4170 4543 1563
Size: 2607 373 3063
Fragments arranged by size:
3063 2607 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2832 4106 5490 5758 1632
Size: 1200 1274 1384 268 1917
Fragments arranged by size:
1917 1384 1274 1200 268
AfeI AGC'GCT
Cuts at: 3088 3088
Size: 6043
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5846
AflIII A'CryG_T
Cuts at: 1203 4942 5919 1203
Size: 3739 977 1327
Fragments arranged by size:
3739 1327 977
AgeI A'CCGG_T
Cuts at: 4874 4874
Size: 6043
AhdI GACnn_n'nnGTC
Cuts at: 3182 4772 3182
Size: 1590 4453
AleI CACnn'nnGTG
Cuts at: 1166 2579 1166
Size: 1413 4630
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6043
AlwNI CAG_nnn'CTG
Cuts at: 2053 2878 3786 5358 2053
Size: 825 908 1572 2738
Fragments arranged by size:
2738 1572 908 825
ApaLI G'TGCA_C
Cuts at: 3099 4100 5256 3099
Size: 1001 1156 3886
Fragments arranged by size:
3886 1156 1001
ApoI r'AATT_y
Cuts at: 1493 2321 3352 3578 3730 5839 1493
Size: 828 1031 226 152 2109 1697
Fragments arranged by size:
2109 1697 1031 828 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4597 160
Size: 872 3565 1606
Fragments arranged by size:
3565 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6043
AvaI C'yCGr_G
Cuts at: 1870 1943 2466 2748 2901 3368 1870
Size: 73 523 282 153 467 4545
Fragments arranged by size:
4545 523 467 282 153 73
AvrII C'CTAG_G
Cuts at: 3014 3116 3014
Size: 102 5941
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2015 2048 2015
Size: 33 6010
BamHI G'GATC_C
Cuts at: 3373 3740 3373
Size: 367 5676
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2198 2441 2610 2641 3364
Size: 325 217 627 411 243 169 31 723
Cuts at: 3364 4819 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 723 627 411 325 243 217
169 31
BanII G_rGCy'C
Cuts at: 1391 2286 2757 3362 1391
Size: 895 471 605 4072
Fragments arranged by size:
4072 895 605 471
BbeI G_GCGC'C
Cuts at: 2202 2445 2614 2645 2202
Size: 243 169 31 5600
Fragments arranged by size:
5600 243 169 31
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2781 2820 3204 928
Size: 971 882 39 384 3767
Fragments arranged by size:
3767 971 882 384 39
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4234 4268 1903
Size: 34 2297 34 3678
Fragments arranged by size:
3678 2297 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3935 5145 3935
Size: 1210 4833
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6043
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4654 136
Size: 107 122 71 631 605 2982 1525
Fragments arranged by size:
2982 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6038 6038
Size: 6043
BlpI GC'TnA_GC
Cuts at: 3460 3460
Size: 6043
Bme1580I G_kGCm'C
Cuts at: 1790 2305 3103 3287 4104 5260 1790
Size: 515 798 184 817 1156 2573
Fragments arranged by size:
2573 1156 817 798 515 184
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6043
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2041 3774 4732
Size: 416 53 83 399 24 624 1733 958
Cuts at: 4732 442
Size: 1753
Fragments arranged by size:
1753 1733 958 624 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2739 1056
Size: 1683 4360
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2305 2710 2305
Size: 405 5638
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2801 3348 4703 1239
Size: 1562 547 1355 2579
Fragments arranged by size:
2579 1562 1355 547
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2471 2600 4168 5033 5331 2471
Size: 129 1568 865 298 3183
Fragments arranged by size:
3183 1568 865 298 129
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2465 3066 4706 882
Size: 617 966 601 1640 2219
Fragments arranged by size:
2219 1640 966 617 601
BsaAI yAC'GTr
Cuts at: 493 1204 3834 493
Size: 711 2630 2702
Fragments arranged by size:
2702 2630 711
BsaBI GATnn'nnATC
Cuts at: 6037 6037
Size: 6043
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2057 2199 2442 2611
Size: 53 83 186 936 524 142 243 169
Cuts at: 2611 2642 3850 4232 4867 275
Size: 31 1208 382 635 1451
Fragments arranged by size:
1451 1208 936 635 524 382 243 186
169 142 83 53 31
BsaWI w'CCGG_w
Cuts at: 2188 2969 4476 4874 5148 5295 5706 2188
Size: 781 1507 398 274 147 411 2525
Fragments arranged by size:
2525 1507 781 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5818
Fragments arranged by size:
5818 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2678 2697 2702 2804 2807 2810 2813 2816
Size: 1492 19 5 102 3 3 3 3
Cuts at: 2816 2834 3307 1186
Size: 18 473 3922
Fragments arranged by size:
3922 1492 473 102 19 18 5 3
3 3 3
BseYI C'CCAG_C
Cuts at: 2131 2173 2194 2837 3106 5246 5742 2131
Size: 42 21 643 269 2140 496 2432
Fragments arranged by size:
2432 2140 643 496 269 42 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3329 1163
Size: 573 1593 3877
Fragments arranged by size:
3877 1593 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2189 2235 2449 3493 4254 5282
Size: 275 186 188 46 214 1044 761 1028
Cuts at: 5282 1540
Size: 2301
Fragments arranged by size:
2301 1044 1028 761 275 214 188 186
46
BsiHKAI G_wGCw'C
Cuts at: 2224 2508 3103 4104 4189 5260 2224
Size: 284 595 1001 85 1071 3007
Fragments arranged by size:
3007 1071 1001 595 284 85
BsmI GAATG_Cn'
Cuts at: 3554 3647 3554
Size: 93 5950
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2224 2286 2305 2508 2757 3103 3287
Size: 399 434 62 19 203 249 346 184
Cuts at: 3287 3362 4104 4189 5260 1391
Size: 75 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 434 399 346 249 203
184 85 75 62 19
BspEI T'CCGG_A
Cuts at: 2969 5706 2969
Size: 2737 3306
BspHI T'CATG_A
Cuts at: 3932 5662 3932
Size: 1730 4313
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2274 3329 3398 844
Size: 1430 1055 69 3489
Fragments arranged by size:
3489 1430 1055 69
BsrBI CCG'CTC
Cuts at: 1394 1823 2141 2389 3930 1394
Size: 429 318 248 1541 3507
Fragments arranged by size:
3507 1541 429 318 248
BsrDI GCAATG_nn'
Cuts at: 65 1248 4538 4712 65
Size: 1183 3290 174 1396
Fragments arranged by size:
3290 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2444 2607 4687 4874 1700
Size: 110 634 163 2080 187 2869
Fragments arranged by size:
2869 2080 634 187 163 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6043
BssHII G'CGCG_C
Cuts at: 1925 2868 1925
Size: 943 5100
BssSI C'ACGA_G
Cuts at: 4103 5115 4103
Size: 1012 5031
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2079 2228 2878 2928 794
Size: 1285 149 650 50 3909
Fragments arranged by size:
3909 1285 650 149 50
BstBI TT'CG_AA
Cuts at: 4847 4847
Size: 6043
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6043
BstYI r'GATC_y
Cuts at: 1972 3373 3740 4125 4142 5594 5680 5692 6038
Size: 1401 367 385 17 1452 86 12 346
Cuts at: 6038 1972
Size: 1977
Fragments arranged by size:
1977 1452 1401 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2035 3287 513
Size: 552 970 1252 3269
Fragments arranged by size:
3269 1252 970 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5304
Fragments arranged by size:
5304 683 56
BtsI GCAGTG_nn'
Cuts at: 2185 2433 2876 3317 3559 4353 4373 2185
Size: 248 443 441 242 794 20 3855
Fragments arranged by size:
3855 794 443 441 248 242 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6043
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6008
DraI TTT'AAA
Cuts at: 3347 3347
Size: 6043
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5050 809
Size: 507 3734 1802
Fragments arranged by size:
3734 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2232 2550 2829
Size: 54 1425 50 275 18 402 318 279
Cuts at: 2829 3490 4379 6008 8
Size: 661 889 1629 43
Fragments arranged by size:
1629 1425 889 661 402 318 279 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2232 3490 1537
Size: 275 420 1258 4090
Fragments arranged by size:
4090 1258 420 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2781 3973 1353
Size: 1428 1192 3423
Fragments arranged by size:
3423 1428 1192
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3014 4630 5004 5150 387
Size: 1064 138 1425 1616 374 146 1280
Fragments arranged by size:
1616 1425 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2801 2832 3348 4106 4703 5490 5758
Size: 393 1169 31 516 758 597 787 268
Cuts at: 5758 1239
Size: 1524
Fragments arranged by size:
1524 1169 787 758 597 516 393 268
31
EcoNI CCTnn'n_nnAGG
Cuts at: 2576 2576
Size: 6043
EcoRI G'AATT_C
Cuts at: 3352 3730 3352
Size: 378 5665
FspI TGC'GCA
Cuts at: 2152 4549 2152
Size: 2397 3646
HaeII r_GCGC'y
Cuts at: 1678 2043 2138 2202 2445 2614 2645 3090 5190
Size: 365 95 64 243 169 31 445 2100
Cuts at: 5190 1678
Size: 2531
Fragments arranged by size:
2531 2100 445 365 243 169 95 64
31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2102 2134 2978 3010 3168
Size: 32 135 32 530 32 844 32 158
Cuts at: 3168 3200 4684 4716 4758 4790 1373
Size: 32 1484 32 42 32 2626
Fragments arranged by size:
2626 1484 844 530 158 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2168 3021 3387 3633 669
Size: 988 281 230 853 366 246 3079
Fragments arranged by size:
3079 988 853 366 281 246 230
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6043
HpaI GTT'AAC
Cuts at: 3633 3633
Size: 6043
KasI G'GCGC_C
Cuts at: 2198 2441 2610 2641 2198
Size: 243 169 31 5600
Fragments arranged by size:
5600 243 169 31
KpnI G_GTAC'C
Cuts at: 3368 3368
Size: 6043
MfeI C'AATT_G
Cuts at: 3642 3642
Size: 6043
MluI A'CGCG_T
Cuts at: 5919 5919
Size: 6043
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5157 5341 1305
Size: 3852 184 2007
Fragments arranged by size:
3852 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2552 2831 10
Size: 54 1425 343 720 279 3222
Fragments arranged by size:
3222 1425 720 343 279 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2564 2579 4001 4360 4519 518
Size: 648 1398 15 1422 359 159 2042
Fragments arranged by size:
2042 1422 1398 648 359 159 15
MspA1I CmG'CkG
Cuts at: 1606 2037 2237 2243 2837 3268 3289 3459 4136
Size: 431 200 6 594 431 21 170 677
Cuts at: 4136 5284 5529 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 594 431 431 245 200
170 21 6
NaeI GCC'GGC
Cuts at: 2609 2609
Size: 6043
NarI GG'CG_CC
Cuts at: 2199 2442 2611 2642 2199
Size: 243 169 31 5600
Fragments arranged by size:
5600 243 169 31
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5491
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6043
NgoMIV G'CCGG_C
Cuts at: 2607 2607
Size: 6043
NheI G'CTAG_C
Cuts at: 1052 2735 1052
Size: 1683 4360
NotI GC'GGCC_GC
Cuts at: 2232 3490 2232
Size: 1258 4785
NspI r_CATG'y
Cuts at: 1132 1229 2082 3401 4946 1132
Size: 97 853 1319 1545 2229
Fragments arranged by size:
2229 1545 1319 853 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6043
PciI A'CATG_T
Cuts at: 4942 4942
Size: 6043
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2021 2053 1108
Size: 913 32 5098
Fragments arranged by size:
5098 913 32
PfoI T'CCnGG_A
Cuts at: 3148 3148
Size: 6043
PmeI GTTT'AAAC
Cuts at: 3347 3347
Size: 6043
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4112 4144 5625 5657 4112
Size: 32 1481 32 4498
Fragments arranged by size:
4498 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6043
PsiI TTA'TAA
Cuts at: 3613 3613
Size: 6043
PstI C_TGCA'G
Cuts at: 830 2242 2338 2899 3258 3395 830
Size: 1412 96 561 359 137 3478
Fragments arranged by size:
3478 1412 561 359 137 96
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6043
PvuII CAG'CTG
Cuts at: 2243 2837 2243
Size: 594 5449
SacII CC_GC'GG
Cuts at: 2038 3290 2038
Size: 1252 4791
SalI G'TCGA_C
Cuts at: 1936 2166 3385 1936
Size: 230 1219 4594
Fragments arranged by size:
4594 1219 230
SbfI CC_TGCA'GG
Cuts at: 3258 3395 3258
Size: 137 5906
ScaI AGT'ACT
Cuts at: 4291 6003 4291
Size: 1712 4331
SexAI A'CCwGG_T
Cuts at: 2588 2588
Size: 6043
SfcI C'TryA_G
Cuts at: 826 1045 2238 2272 2334 2895 3254 3303 3391
Size: 219 1193 34 62 561 359 49 88
Cuts at: 3391 4526 5207 5398 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1193 1135 681 561 359 219 191
88 62 49 34
SfoI GGC'GCC
Cuts at: 2200 2443 2612 2643 2200
Size: 243 169 31 5600
Fragments arranged by size:
5600 243 169 31
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6043
SgrAI Cr'CCGG_yG
Cuts at: 1700 2607 1700
Size: 907 5136
SmaI CCC'GGG
Cuts at: 1872 2468 2750 3370 1872
Size: 596 282 620 4545
Fragments arranged by size:
4545 620 596 282
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2486 2615 2901 4147 5048 5310
Size: 197 926 543 129 286 1246 901 262
Cuts at: 5310 820
Size: 1553
Fragments arranged by size:
1553 1246 926 901 543 286 262 197
129
SnaBI TAC'GTA
Cuts at: 493 3834 493
Size: 3341 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6043
SphI G_CATG'C
Cuts at: 1132 3401 1132
Size: 2269 3774
SspI AAT'ATT
Cuts at: 5 52 3967 5
Size: 47 3915 2081
Fragments arranged by size:
3915 2081 47
StuI AGG'CCT
Cuts at: 1517 2492 1517
Size: 975 5068
StyI C'CwwG_G
Cuts at: 513 1065 2205 3014 3116 3482 513
Size: 552 1140 809 102 366 3074
Fragments arranged by size:
3074 1140 809 552 366 102
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2591 4070 4087 4240 4425 4870 325
Size: 1255 1011 1479 17 153 185 445 1498
Fragments arranged by size:
1498 1479 1255 1011 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2480 4289 6001
Size: 274 80 33 51 1443 503 1809 1712
Cuts at: 6001 96
Size: 138
Fragments arranged by size:
1809 1712 1443 503 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6003 6035 6003
Size: 32 6011
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6043
XbaI T'CTAG_A
Cuts at: 3379 3379
Size: 6043
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2255 3323 2255
Size: 1068 4975
XhoI C'TCGA_G
Cuts at: 1943 2901 1943
Size: 958 5085
XmaI C'CCGG_G
Cuts at: 1870 2466 2748 3368 1870
Size: 596 282 620 4545
Fragments arranged by size:
4545 620 596 282
XmnI GAAnn'nnTTC
Cuts at: 4172 4172
Size: 6043
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3851 276
Size: 53 83 186 936 2317 2468
Fragments arranged by size:
2468 2317 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI BbvCI BfrBI Bpu10I BsiWI BsmBI
BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI
FseI FspAI NruI NsiI PacI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacI SanDI SapI SfiI SrfI
SwaI