Restriction map for
pFN21AB9728
(Circular) (Six-base) MAPSORT of: pFN21AB9728.seq Check: 9270 from: 1 to: 6037
pFN21AB9728 6037 bp
With 182 enzymes: SgfI *
October 20, 2009 10:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3847 278
Size: 53 83 186 936 2311 2468
Fragments arranged by size:
2468 2311 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3380 1937
Size: 1443 4594
Acc65I G'GTAC_C
Cuts at: 2953 3358 2953
Size: 405 5632
AclI AA'CG_TT
Cuts at: 1563 4164 4537 1563
Size: 2601 373 3063
Fragments arranged by size:
3063 2601 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2575 4100 5484 5752 1632
Size: 943 1525 1384 268 1917
Fragments arranged by size:
1917 1525 1384 943 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5840
AflIII A'CryG_T
Cuts at: 1203 2829 4936 5913 1203
Size: 1626 2107 977 1327
Fragments arranged by size:
2107 1626 1327 977
AgeI A'CCGG_T
Cuts at: 4868 4868
Size: 6037
AhdI GACnn_n'nnGTC
Cuts at: 4766 4766
Size: 6037
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6037
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3078 1184
Size: 1894 4143
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2552 2584 2552
Size: 32 6005
AlwNI CAG_nnn'CTG
Cuts at: 2392 3780 5352 2392
Size: 1388 1572 3077
Fragments arranged by size:
3077 1572 1388
ApaLI G'TGCA_C
Cuts at: 4094 5250 4094
Size: 1156 4881
ApoI r'AATT_y
Cuts at: 1493 3346 3572 3724 5833 1493
Size: 1853 226 152 2109 1697
Fragments arranged by size:
2109 1853 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4591 160
Size: 872 3559 1606
Fragments arranged by size:
3559 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6037
AvaI C'yCGr_G
Cuts at: 1870 1943 2281 2297 2498 2842 2885 3362 1870
Size: 73 338 16 201 344 43 477 4545
Fragments arranged by size:
4545 477 344 338 201 73 43 16
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2945 2978 2945
Size: 33 6004
BamHI G'GATC_C
Cuts at: 3367 3734 3367
Size: 367 5670
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2249 2264 2420 2689 2701
Size: 325 217 627 462 15 156 269 12
Cuts at: 2701 2777 2872 2953 3035 3358 4813 618
Size: 76 95 81 82 323 1455 1842
Fragments arranged by size:
1842 1455 627 462 325 323 269 217
156 95 82 81 76 15 12
BanII G_rGCy'C
Cuts at: 1391 2093 2282 2351 2567 3011 3029 3356 1391
Size: 702 189 69 216 444 18 327 4072
Fragments arranged by size:
4072 702 444 327 216 189 69 18
BbeI G_GCGC'C
Cuts at: 2253 2268 2424 2693 2705 2781 3039 2253
Size: 15 156 269 12 76 258 5251
Fragments arranged by size:
5251 269 258 156 76 15 12
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5066
BbvCI CC'TCA_GC
Cuts at: 3268 3268
Size: 6037
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4228 4262 1903
Size: 34 2291 34 3678
Fragments arranged by size:
3678 2291 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3929 5139 3929
Size: 1210 4827
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6037
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2213 2273 2727
Size: 107 122 71 631 605 541 60 454
Cuts at: 2727 3095 4648 136
Size: 368 1553 1525
Fragments arranged by size:
1553 1525 631 605 541 454 368 122
107 71 60
BglII A'GATC_T
Cuts at: 2379 6032 2379
Size: 3653 2384
BlpI GC'TnA_GC
Cuts at: 2372 3454 2372
Size: 1082 4955
Bme1580I G_kGCm'C
Cuts at: 1790 2877 4098 5254 1790
Size: 1087 1221 1156 2573
Fragments arranged by size:
2573 1221 1156 1087
BmgBI CAC'GTC
Cuts at: 1318 2821 1318
Size: 1503 4534
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3768 4726 442
Size: 416 53 83 399 24 2351 958 1753
Fragments arranged by size:
2351 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3276 1056
Size: 2220 3817
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2155 2581 3187 2155
Size: 426 606 5005
Fragments arranged by size:
5005 606 426
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2057 3043 3094 3152 3192 4697 1239
Size: 818 986 51 58 40 1505 2579
Fragments arranged by size:
2579 1505 986 818 58 51 40
Bpu10I CC'TnA_GC
Cuts at: 3268 3268
Size: 6037
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2597 3035 4162 5027 5325 2597
Size: 438 1127 865 298 3309
Fragments arranged by size:
3309 1127 865 438 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2126 4700 882
Size: 617 627 2574 2219
Fragments arranged by size:
2574 2219 627 617
BsaAI yAC'GTr
Cuts at: 493 1204 3828 493
Size: 711 2624 2702
Fragments arranged by size:
2702 2624 711
BsaBI GATnn'nnATC
Cuts at: 6031 6031
Size: 6037
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2250 2265 2421 2657
Size: 53 83 186 936 717 15 156 236
Cuts at: 2657 2690 2702 2766 2778 2892 3036 3071 3844
Size: 33 12 64 12 114 144 35 773
Cuts at: 3844 4226 4861 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 773 717 635 382 236 186
156 144 114 83 64 53 35 33
15 12 12
BsaWI w'CCGG_w
Cuts at: 4470 4868 5142 5289 5700 4470
Size: 398 274 147 411 4807
Fragments arranged by size:
4807 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2552 2582 1178
Size: 30 165 30 1149 30 4633
Fragments arranged by size:
4633 1149 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2131 2134 2137 2179 2573 1186
Size: 945 3 3 42 394 4650
Fragments arranged by size:
4650 945 394 42 3 3
BseYI C'CCAG_C
Cuts at: 2354 2783 3074 5240 5736 2354
Size: 429 291 2166 496 2655
Fragments arranged by size:
2655 2166 496 429 291
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2071 3089 1163
Size: 573 335 1018 4111
Fragments arranged by size:
4111 1018 573 335
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2582 2904 3487 4248 5276 1540
Size: 275 186 581 322 583 761 1028 2301
Fragments arranged by size:
2301 1028 761 583 581 322 275 186
BsiHKAI G_wGCw'C
Cuts at: 2282 2351 3029 4098 4183 5254 2282
Size: 69 678 1069 85 1071 3065
Fragments arranged by size:
3065 1071 1069 678 85 69
BsmI GAATG_Cn'
Cuts at: 2993 3548 3641 2993
Size: 555 93 5389
Fragments arranged by size:
5389 555 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2093 2282 2351 2567 2877 3011 3029
Size: 399 303 189 69 216 310 134 18
Cuts at: 3029 3356 4098 4183 5254 1391
Size: 327 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 327 310 303 216
189 134 85 69 18
BspEI T'CCGG_A
Cuts at: 5700 5700
Size: 6037
BspHI T'CATG_A
Cuts at: 3926 5656 3926
Size: 1730 4307
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3392 844
Size: 2548 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2038 2259 2640 3924 1394
Size: 429 215 221 381 1284 3507
Fragments arranged by size:
3507 1284 429 381 221 215
BsrDI GCAATG_nn'
Cuts at: 65 1248 4532 4706 65
Size: 1183 3284 174 1396
Fragments arranged by size:
3284 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2029 2577 2768 4681 4868 1700
Size: 110 219 548 191 1913 187 2869
Fragments arranged by size:
2869 1913 548 219 191 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6037
BssHII G'CGCG_C
Cuts at: 1925 2067 2399 1925
Size: 142 332 5563
Fragments arranged by size:
5563 332 142
BssSI C'ACGA_G
Cuts at: 3004 4097 5109 3004
Size: 1093 1012 3932
Fragments arranged by size:
3932 1093 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6037
BstBI TT'CG_AA
Cuts at: 4841 4841
Size: 6037
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6037
BstXI CCAn_nnnn'nTGG
Cuts at: 2857 2857
Size: 6037
BstYI r'GATC_y
Cuts at: 1972 2379 3367 3734 4119 4136 5588 5674 5686
Size: 407 988 367 385 17 1452 86 12
Cuts at: 5686 6032 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 988 407 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2490 2490
Size: 6037
BtgI C'CryG_G
Cuts at: 513 1065 2416 2754 2877 2970 513
Size: 552 1351 338 123 93 3580
Fragments arranged by size:
3580 1351 552 338 123 93
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2305 487
Size: 683 56 1079 4219
Fragments arranged by size:
4219 1079 683 56
BtsI GCAGTG_nn'
Cuts at: 3553 4347 4367 3553
Size: 794 20 5223
Fragments arranged by size:
5223 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6037
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6002
DraI TTT'AAA
Cuts at: 3341 3341
Size: 6037
DraIII CAC_nnn'GTG
Cuts at: 2632 2632
Size: 6037
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5044 809
Size: 507 3728 1802
Fragments arranged by size:
3728 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2199 2337 2538
Size: 54 1425 50 275 18 369 138 201
Cuts at: 2538 2579 2859 2901 2967 3484 4373 6002 8
Size: 41 280 42 66 517 889 1629 43
Fragments arranged by size:
1629 1425 889 517 369 280 275 201
138 66 54 50 43 42 41 18
EagI C'GGCC_G
Cuts at: 1537 1812 2579 2901 3484 1537
Size: 275 767 322 583 4090
Fragments arranged by size:
4090 767 583 322 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3967 1353
Size: 2614 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2030 2152 2561 2611 4624 4998
Size: 1064 138 441 122 409 50 2013 374
Cuts at: 4998 5144 387
Size: 146 1280
Fragments arranged by size:
2013 1280 1064 441 409 374 146 138
122 50
EcoICRI GAG'CTC
Cuts at: 2280 2349 3027 2280
Size: 69 678 5290
Fragments arranged by size:
5290 678 69
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2057 2575 3043 3094 3152 3192 4100
Size: 393 425 518 468 51 58 40 908
Cuts at: 4100 4697 5484 5752 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 908 787 597 518 468 425 393
268 58 51 40
EcoO109I rG'GnC_Cy
Cuts at: 2494 2882 3166 2494
Size: 388 284 5365
Fragments arranged by size:
5365 388 284
EcoRI G'AATT_C
Cuts at: 3346 3724 3346
Size: 378 5659
FspI TGC'GCA
Cuts at: 4543 4543
Size: 6037
HaeII r_GCGC'y
Cuts at: 1678 2208 2253 2268 2365 2424 2612 2693 2705
Size: 530 45 15 97 59 188 81 12
Cuts at: 2705 2781 2982 3039 5184 1678
Size: 76 201 57 2145 2531
Fragments arranged by size:
2531 2145 530 201 188 97 81 76
59 57 45 15 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3295 3327 4678 4710 4752
Size: 32 135 32 1723 32 1351 32 42
Cuts at: 4752 4784 1373
Size: 32 2626
Fragments arranged by size:
2626 1723 1351 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3381 3627 669
Size: 988 281 1443 246 3079
Fragments arranged by size:
3079 1443 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6037
HpaI GTT'AAC
Cuts at: 3627 3627
Size: 6037
KasI G'GCGC_C
Cuts at: 2249 2264 2420 2689 2701 2777 3035 2249
Size: 15 156 269 12 76 258 5251
Fragments arranged by size:
5251 269 258 156 76 15 12
KpnI G_GTAC'C
Cuts at: 2957 3362 2957
Size: 405 5632
MfeI C'AATT_G
Cuts at: 3636 3636
Size: 6037
MluI A'CGCG_T
Cuts at: 5913 5913
Size: 6037
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5151 5335 1305
Size: 3846 184 2007
Fragments arranged by size:
3846 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2339 2861 10
Size: 54 1425 343 507 522 3186
Fragments arranged by size:
3186 1425 522 507 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2333 2855 3995 4354 4513 518
Size: 648 1167 522 1140 359 159 2042
Fragments arranged by size:
2042 1167 1140 648 522 359 159
MspA1I CmG'CkG
Cuts at: 1606 2418 2756 2865 2879 3453 4130 5278 5523
Size: 812 338 109 14 574 677 1148 245
Cuts at: 5523 1606
Size: 2120
Fragments arranged by size:
2120 1148 812 677 574 338 245 109
14
NaeI GCC'GGC
Cuts at: 2031 2579 2770 2031
Size: 548 191 5298
Fragments arranged by size:
5298 548 191
NarI GG'CG_CC
Cuts at: 2250 2265 2421 2690 2702 2778 3036 2250
Size: 15 156 269 12 76 258 5251
Fragments arranged by size:
5251 269 258 156 76 15 12
NcoI C'CATG_G
Cuts at: 513 1065 2970 513
Size: 552 1905 3580
Fragments arranged by size:
3580 1905 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6037
NgoMIV G'CCGG_C
Cuts at: 2029 2577 2768 2029
Size: 548 191 5298
Fragments arranged by size:
5298 548 191
NheI G'CTAG_C
Cuts at: 1052 3272 1052
Size: 2220 3817
NotI GC'GGCC_GC
Cuts at: 3484 3484
Size: 6037
NruI TCG'CGA
Cuts at: 3162 3162
Size: 6037
NspI r_CATG'y
Cuts at: 1132 1229 2833 3118 3395 4940 1132
Size: 97 1604 285 277 1545 2229
Fragments arranged by size:
2229 1604 1545 285 277 97
PasI CC'CwG_GG
Cuts at: 1348 2671 1348
Size: 1323 4714
PciI A'CATG_T
Cuts at: 2829 4936 2829
Size: 2107 3930
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2632 1108
Size: 1524 4513
PmeI GTTT'AAAC
Cuts at: 3341 3341
Size: 6037
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2537 2552 2569 2584 4106 4138 5619 5651 2537
Size: 15 17 15 1522 32 1481 32 2923
Fragments arranged by size:
2923 1522 1481 32 32 17 15 15
PpuMI rG'GwC_Cy
Cuts at: 2882 3166 2882
Size: 284 5753
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6037
PsiI TTA'TAA
Cuts at: 3607 3607
Size: 6037
PstI C_TGCA'G
Cuts at: 830 2397 3389 830
Size: 1567 992 3478
Fragments arranged by size:
3478 1567 992
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6037
PvuII CAG'CTG
Cuts at: 2865 2865
Size: 6037
SacI G_AGCT'C
Cuts at: 2282 2351 3029 2282
Size: 69 678 5290
Fragments arranged by size:
5290 678 69
SacII CC_GC'GG
Cuts at: 2419 2757 2880 2419
Size: 338 123 5576
Fragments arranged by size:
5576 338 123
SalI G'TCGA_C
Cuts at: 1936 3379 1936
Size: 1443 4594
SanDI GG'GwC_CC
Cuts at: 2882 2882
Size: 6037
SbfI CC_TGCA'GG
Cuts at: 2397 3389 2397
Size: 992 5045
ScaI AGT'ACT
Cuts at: 4285 5997 4285
Size: 1712 4325
SfcI C'TryA_G
Cuts at: 826 1045 2393 3385 4520 5201 5392 826
Size: 219 1348 992 1135 681 191 1471
Fragments arranged by size:
1471 1348 1135 992 681 219 191
SfoI GGC'GCC
Cuts at: 2251 2266 2422 2691 2703 2779 3037 2251
Size: 15 156 269 12 76 258 5251
Fragments arranged by size:
5251 269 258 156 76 15 12
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6037
SgrAI Cr'CCGG_yG
Cuts at: 1700 2768 1700
Size: 1068 4969
SmaI CCC'GGG
Cuts at: 1872 2844 2887 3364 1872
Size: 972 43 477 4545
Fragments arranged by size:
4545 972 477 43
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2612 3050 4141 5042 5304 820
Size: 197 926 669 438 1091 901 262 1553
Fragments arranged by size:
1553 1091 926 901 669 438 262 197
SnaBI TAC'GTA
Cuts at: 493 3828 493
Size: 3335 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6037
SphI G_CATG'C
Cuts at: 1132 3395 1132
Size: 2263 3774
SspI AAT'ATT
Cuts at: 5 52 3961 5
Size: 47 3909 2081
Fragments arranged by size:
3909 2081 47
StuI AGG'CCT
Cuts at: 1517 3102 1517
Size: 1585 4452
StyI C'CwwG_G
Cuts at: 513 1065 2970 3476 513
Size: 552 1905 506 3074
Fragments arranged by size:
3074 1905 552 506
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4064 4081 4234 4419 4864 325
Size: 1255 2484 17 153 185 445 1498
Fragments arranged by size:
2484 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4283 5995 96
Size: 274 80 33 51 1443 2306 1712 138
Fragments arranged by size:
2306 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5997 6029 5997
Size: 32 6005
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6037
XbaI T'CTAG_A
Cuts at: 3373 3373
Size: 6037
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6037
XmaI C'CCGG_G
Cuts at: 1870 2842 2885 3362 1870
Size: 972 43 477 4545
Fragments arranged by size:
4545 972 477 43
XmnI GAAnn'nnTTC
Cuts at: 4166 4166
Size: 6037
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3845 276
Size: 53 83 186 936 2311 2468
Fragments arranged by size:
2468 2311 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacI SacII SalI
SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BfrBI BsiWI BsmBI
BstZ17I EcoNI EcoRV FalI FseI FspAI NsiI PacI
PfoI PmlI PspOMI PspXI PsrI RsrII SapI SexAI
SfiI SrfI SwaI XcmI