Restriction map for
pFN21AB9726
(Circular) (Six-base) MAPSORT of: pFN21AB9726.seq Check: 9160 from: 1 to: 5998
pFN21AB9726 5998 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3808 278
Size: 53 83 186 936 2272 2468
Fragments arranged by size:
2468 2272 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3341 1937
Size: 1404 4594
Acc65I G'GTAC_C
Cuts at: 3319 3319
Size: 5998
AclI AA'CG_TT
Cuts at: 1563 4125 4498 1563
Size: 2562 373 3063
Fragments arranged by size:
3063 2562 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4061 5445 5713 1632
Size: 2429 1384 268 1917
Fragments arranged by size:
2429 1917 1384 268
AfeI AGC'GCT
Cuts at: 2150 2150
Size: 5998
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5801
AflIII A'CryG_T
Cuts at: 1203 4897 5874 1203
Size: 3694 977 1327
Fragments arranged by size:
3694 1327 977
AgeI A'CCGG_T
Cuts at: 4829 4829
Size: 5998
AhdI GACnn_n'nnGTC
Cuts at: 4727 4727
Size: 5998
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5998
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5998
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2606 2638 2606
Size: 32 5966
AlwNI CAG_nnn'CTG
Cuts at: 2173 3741 5313 2173
Size: 1568 1572 2858
Fragments arranged by size:
2858 1572 1568
ApaI G_GGCC'C
Cuts at: 2331 2780 2331
Size: 449 5549
ApaLI G'TGCA_C
Cuts at: 4055 5211 4055
Size: 1156 4842
ApoI r'AATT_y
Cuts at: 1493 3307 3533 3685 5794 1493
Size: 1814 226 152 2109 1697
Fragments arranged by size:
2109 1814 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4552 160
Size: 872 3520 1606
Fragments arranged by size:
3520 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5998
AvaI C'yCGr_G
Cuts at: 1870 1943 2243 2551 2984 3323 1870
Size: 73 300 308 433 339 4545
Fragments arranged by size:
4545 433 339 308 300 73
BamHI G'GATC_C
Cuts at: 3082 3328 3695 3082
Size: 246 367 5385
Fragments arranged by size:
5385 367 246
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2363 2951 2980 3020 3169
Size: 325 217 627 576 588 29 40 149
Cuts at: 3169 3181 3319 4774 618
Size: 12 138 1455 1842
Fragments arranged by size:
1842 1455 627 588 576 325 217 149
138 40 29 12
BanII G_rGCy'C
Cuts at: 1391 2211 2250 2331 2549 2780 2870 3317 1391
Size: 820 39 81 218 231 90 447 4072
Fragments arranged by size:
4072 820 447 231 218 90 81 39
BbeI G_GCGC'C
Cuts at: 2955 2984 3024 3173 3185 2955
Size: 29 40 149 12 5768
Fragments arranged by size:
5768 149 40 29 12
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5027
BbvCI CC'TCA_GC
Cuts at: 2684 2684
Size: 5998
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2065 2099 4189 4223 1903
Size: 34 50 34 44 34 2090 34 3678
Fragments arranged by size:
3678 2090 50 44 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3890 5100 3890
Size: 1210 4788
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5998
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4609 136
Size: 107 122 71 631 605 2937 1525
Fragments arranged by size:
2937 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2703 5993 2703
Size: 3290 2708
BlpI GC'TnA_GC
Cuts at: 2152 3415 2152
Size: 1263 4735
Bme1580I G_kGCm'C
Cuts at: 1790 2331 2780 4059 5215 1790
Size: 541 449 1279 1156 2573
Fragments arranged by size:
2573 1279 1156 541 449
BmgBI CAC'GTC
Cuts at: 1318 3001 1318
Size: 1683 4315
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2274 3729 4687
Size: 416 53 83 399 24 857 1455 958
Cuts at: 4687 442
Size: 1753
Fragments arranged by size:
1753 1455 958 857 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5998
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2153 2633 2769 4658 1239
Size: 914 480 136 1889 2579
Fragments arranged by size:
2579 1889 914 480 136
Bpu10I CC'TnA_GC
Cuts at: 2684 2684
Size: 5998
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4123 4988 5286 4123
Size: 865 298 4835
Fragments arranged by size:
4835 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4661 882
Size: 617 3162 2219
Fragments arranged by size:
3162 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3061 3789 493
Size: 711 1857 728 2702
Fragments arranged by size:
2702 1857 728 711
BsaBI GATnn'nnATC
Cuts at: 2702 5992 2702
Size: 3290 2708
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2564 2952 2981 3021
Size: 53 83 186 936 1031 388 29 40
Cuts at: 3021 3032 3170 3182 3805 4187 4822 275
Size: 11 138 12 623 382 635 1451
Fragments arranged by size:
1451 1031 936 635 623 388 382 186
138 83 53 40 29 12 11
BsaWI w'CCGG_w
Cuts at: 3052 4431 4829 5103 5250 5661 3052
Size: 1379 398 274 147 411 3389
Fragments arranged by size:
3389 1379 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2606 2636 1178
Size: 30 165 30 1203 30 4540
Fragments arranged by size:
4540 1203 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3226 3243 3246 1186
Size: 2040 17 3 3938
Fragments arranged by size:
3938 2040 17 3
BseYI C'CCAG_C
Cuts at: 2330 2855 3185 5201 5697 2330
Size: 525 330 2016 496 2631
Fragments arranged by size:
2631 2016 525 496 330
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5425
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2823 3448 4209 5237 1540
Size: 275 186 822 625 761 1028 2301
Fragments arranged by size:
2301 1028 822 761 625 275 186
BsiHKAI G_wGCw'C
Cuts at: 2211 4059 4144 5215 2211
Size: 1848 85 1071 2994
Fragments arranged by size:
2994 1848 1071 85
BsmI GAATG_Cn'
Cuts at: 2908 3509 3602 2908
Size: 601 93 5304
Fragments arranged by size:
5304 601 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2211 2250 2331 2549 2780 2870 3317
Size: 399 421 39 81 218 231 90 447
Cuts at: 3317 4059 4144 5215 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 447 421 399 231 218
90 85 81 39
BspEI T'CCGG_A
Cuts at: 5661 5661
Size: 5998
BspHI T'CATG_A
Cuts at: 2829 3887 5617 2829
Size: 1058 1730 3210
Fragments arranged by size:
3210 1730 1058
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2682 3353 844
Size: 1838 671 3489
Fragments arranged by size:
3489 1838 671
BsrBI CCG'CTC
Cuts at: 1394 1823 2692 3885 1394
Size: 429 869 1193 3507
Fragments arranged by size:
3507 1193 869 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2083 4493 4667 65
Size: 1183 835 2410 174 1396
Fragments arranged by size:
2410 1396 1183 835 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2646 2718 2789 2822 3101 4642 4829
Size: 110 836 72 71 33 279 1541 187
Cuts at: 4829 1700
Size: 2869
Fragments arranged by size:
2869 1541 836 279 187 110 72 71
33
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5998
BssHII G'CGCG_C
Cuts at: 1925 2595 1925
Size: 670 5328
BssSI C'ACGA_G
Cuts at: 2590 4058 5070 2590
Size: 1468 1012 3518
Fragments arranged by size:
3518 1468 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2809 794
Size: 2015 3983
BstBI TT'CG_AA
Cuts at: 4802 4802
Size: 5998
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5998
BstXI CCAn_nnnn'nTGG
Cuts at: 2131 2758 2131
Size: 627 5371
BstYI r'GATC_y
Cuts at: 1972 2703 3082 3328 3695 4080 4097 5549 5635
Size: 731 379 246 367 385 17 1452 86
Cuts at: 5635 5647 5993 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 731 385 379 367 346 246
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3258 3258
Size: 5998
BtgI C'CryG_G
Cuts at: 513 1065 2034 2817 3196 513
Size: 552 969 783 379 3315
Fragments arranged by size:
3315 969 783 552 379
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5259
Fragments arranged by size:
5259 683 56
BtsI GCAGTG_nn'
Cuts at: 2503 3514 4308 4328 2503
Size: 1011 794 20 4173
Fragments arranged by size:
4173 1011 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5998
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5963
DraI TTT'AAA
Cuts at: 3302 3302
Size: 5998
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5005 809
Size: 507 3689 1802
Fragments arranged by size:
3689 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2667 2820 3445
Size: 54 1425 50 275 18 837 153 625
Cuts at: 3445 4334 5963 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 837 625 275 153 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2820 3445 1537
Size: 275 1008 625 4090
Fragments arranged by size:
4090 1008 625 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2051 3928 1353
Size: 698 1877 3423
Fragments arranged by size:
3423 1877 698
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2505 4585 4959 5105 387
Size: 1064 138 916 2080 374 146 1280
Fragments arranged by size:
2080 1280 1064 916 374 146 138
EcoICRI GAG'CTC
Cuts at: 2209 2209
Size: 5998
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2153 2633 2769 4061 4658 5445 5713
Size: 393 521 480 136 1292 597 787 268
Cuts at: 5713 1239
Size: 1524
Fragments arranged by size:
1524 1292 787 597 521 480 393 268
136
EcoNI CCTnn'n_nnAGG
Cuts at: 2940 2940
Size: 5998
EcoO109I rG'GnC_Cy
Cuts at: 2776 2776
Size: 5998
EcoRI G'AATT_C
Cuts at: 3307 3685 3307
Size: 378 5620
FspI TGC'GCA
Cuts at: 4504 4504
Size: 5998
HaeII r_GCGC'y
Cuts at: 1678 2152 2955 2984 3024 3173 3185 3281 5145
Size: 474 803 29 40 149 12 96 1864
Cuts at: 5145 1678
Size: 2531
Fragments arranged by size:
2531 1864 803 474 149 96 40 29
12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2218 2237 2250 2269 2855
Size: 32 135 32 646 19 13 19 586
Cuts at: 2855 2887 3221 3253 4639 4671 4713 4745 1373
Size: 32 334 32 1386 32 42 32 2626
Fragments arranged by size:
2626 1386 646 586 334 135 42 32
32 32 32 32 32 19 19 13
HincII GTy'rAC
Cuts at: 669 1657 1938 3342 3588 669
Size: 988 281 1404 246 3079
Fragments arranged by size:
3079 1404 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5998
HpaI GTT'AAC
Cuts at: 3588 3588
Size: 5998
KasI G'GCGC_C
Cuts at: 2951 2980 3020 3169 3181 2951
Size: 29 40 149 12 5768
Fragments arranged by size:
5768 149 40 29 12
KpnI G_GTAC'C
Cuts at: 3323 3323
Size: 5998
MfeI C'AATT_G
Cuts at: 3597 3597
Size: 5998
MluI A'CGCG_T
Cuts at: 5874 5874
Size: 5998
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2946 2996 5112 5296 1305
Size: 1641 50 2116 184 2007
Fragments arranged by size:
2116 2007 1641 184 50
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2669 10
Size: 54 1425 343 837 3339
Fragments arranged by size:
3339 1425 837 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2901 3956 4315 4474 518
Size: 648 1735 1055 359 159 2042
Fragments arranged by size:
2042 1735 1055 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2036 2220 2508 2602 3088 3414 4091 5239
Size: 430 184 288 94 486 326 677 1148
Cuts at: 5239 5484 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 486 430 326 288 245
184 94
NaeI GCC'GGC
Cuts at: 2648 2791 2824 3103 2648
Size: 143 33 279 5543
Fragments arranged by size:
5543 279 143 33
NarI GG'CG_CC
Cuts at: 2952 2981 3021 3170 3182 2952
Size: 29 40 149 12 5768
Fragments arranged by size:
5768 149 40 29 12
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5446
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5998
NgoMIV G'CCGG_C
Cuts at: 2646 2789 2822 3101 2646
Size: 143 33 279 5543
Fragments arranged by size:
5543 279 143 33
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5998
NotI GC'GGCC_GC
Cuts at: 3445 3445
Size: 5998
NspI r_CATG'y
Cuts at: 1132 1229 2195 3356 4901 1132
Size: 97 966 1161 1545 2229
Fragments arranged by size:
2229 1545 1161 966 97
PasI CC'CwG_GG
Cuts at: 1348 2173 1348
Size: 825 5173
PciI A'CATG_T
Cuts at: 4897 4897
Size: 5998
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5998
PfoI T'CCnGG_A
Cuts at: 3146 3146
Size: 5998
PmeI GTTT'AAAC
Cuts at: 3302 3302
Size: 5998
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2606 2638 4067 4099 5580 5612 2606
Size: 32 1429 32 1481 32 2992
Fragments arranged by size:
2992 1481 1429 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5998
PsiI TTA'TAA
Cuts at: 3568 3568
Size: 5998
PspOMI G'GGCC_C
Cuts at: 2327 2776 2327
Size: 449 5549
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3143 3175 3143
Size: 32 5966
PstI C_TGCA'G
Cuts at: 830 2169 3350 830
Size: 1339 1181 3478
Fragments arranged by size:
3478 1339 1181
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5998
PvuII CAG'CTG
Cuts at: 2220 2602 2220
Size: 382 5616
SacI G_AGCT'C
Cuts at: 2211 2211
Size: 5998
SacII CC_GC'GG
Cuts at: 2037 2037
Size: 5998
SalI G'TCGA_C
Cuts at: 1936 3340 1936
Size: 1404 4594
SbfI CC_TGCA'GG
Cuts at: 3350 3350
Size: 5998
ScaI AGT'ACT
Cuts at: 4246 5958 4246
Size: 1712 4286
SexAI A'CCwGG_T
Cuts at: 3294 3294
Size: 5998
SfcI C'TryA_G
Cuts at: 826 1045 2165 3346 4481 5162 5353 826
Size: 219 1120 1181 1135 681 191 1471
Fragments arranged by size:
1471 1181 1135 1120 681 219 191
SfoI GGC'GCC
Cuts at: 2953 2982 3022 3171 3183 2953
Size: 29 40 149 12 5768
Fragments arranged by size:
5768 149 40 29 12
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5998
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5998
SmaI CCC'GGG
Cuts at: 1872 2553 3325 1872
Size: 681 772 4545
Fragments arranged by size:
4545 772 681
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4102 5003 5265 820
Size: 197 926 2159 901 262 1553
Fragments arranged by size:
2159 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3789 493
Size: 3296 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5998
SphI G_CATG'C
Cuts at: 1132 3356 1132
Size: 2224 3774
SspI AAT'ATT
Cuts at: 5 52 3922 5
Size: 47 3870 2081
Fragments arranged by size:
3870 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5998
StyI C'CwwG_G
Cuts at: 513 1065 2751 3437 513
Size: 552 1686 686 3074
Fragments arranged by size:
3074 1686 686 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4025 4042 4195 4380 4825 325
Size: 1255 2445 17 153 185 445 1498
Fragments arranged by size:
2445 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2730 4244 5956
Size: 274 80 33 51 1443 753 1514 1712
Cuts at: 5956 96
Size: 138
Fragments arranged by size:
1712 1514 1443 753 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5958 5990 5958
Size: 32 5966
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5998
XbaI T'CTAG_A
Cuts at: 3334 3334
Size: 5998
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5998
XmaI C'CCGG_G
Cuts at: 1870 2551 3323 1870
Size: 681 772 4545
Fragments arranged by size:
4545 772 681
XmnI GAAnn'nnTTC
Cuts at: 2621 4127 2621
Size: 1506 4492
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3806 276
Size: 53 83 186 936 2272 2468
Fragments arranged by size:
2468 2272 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PspOMI PsrI PstI PvuI PvuII SacI SacII
SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI
BstZ17I DraIII EcoRV FalI FseI FspAI NruI NsiI
PacI PmlI PpuMI PspXI RsrII SanDI SapI SfiI
SrfI SwaI XcmI