Restriction map for
pFN21AB9720
(Circular) (Six-base) MAPSORT of: pFN21AB9720.seq Check: 4405 from: 1 to: 5917
pFN21AB9720 5917 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2152 2228 2285 2857 2989 2152
Size: 76 57 572 132 5080
Fragments arranged by size:
5080 572 132 76 57
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3727 278
Size: 53 83 186 936 2191 2468
Fragments arranged by size:
2468 2191 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2895 3260 1937
Size: 958 365 4594
Fragments arranged by size:
4594 958 365
Acc65I G'GTAC_C
Cuts at: 3238 3238
Size: 5917
AclI AA'CG_TT
Cuts at: 1563 4044 4417 1563
Size: 2481 373 3063
Fragments arranged by size:
3063 2481 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2620 3980 5364 5632 1632
Size: 988 1360 1384 268 1917
Fragments arranged by size:
1917 1384 1360 988 268
AfeI AGC'GCT
Cuts at: 3050 3050
Size: 5917
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5720
AflIII A'CryG_T
Cuts at: 1203 2607 4816 5793 1203
Size: 1404 2209 977 1327
Fragments arranged by size:
2209 1404 1327 977
AgeI A'CCGG_T
Cuts at: 4748 4748
Size: 5917
AhdI GACnn_n'nnGTC
Cuts at: 4646 4646
Size: 5917
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5917
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5917
AlwNI CAG_nnn'CTG
Cuts at: 2524 3660 5232 2524
Size: 1136 1572 3209
Fragments arranged by size:
3209 1572 1136
ApaLI G'TGCA_C
Cuts at: 3974 5130 3974
Size: 1156 4761
ApoI r'AATT_y
Cuts at: 1493 3226 3452 3604 5713 1493
Size: 1733 226 152 2109 1697
Fragments arranged by size:
2109 1733 1697 226 152
AscI GG'CGCG_CC
Cuts at: 2654 2654
Size: 5917
AseI AT'TA_AT
Cuts at: 160 1032 4471 160
Size: 872 3439 1606
Fragments arranged by size:
3439 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5917
AvaI C'yCGr_G
Cuts at: 1870 1943 2431 2443 2488 2675 3208 3242 1870
Size: 73 488 12 45 187 533 34 4545
Fragments arranged by size:
4545 533 488 187 73 45 34 12
BamHI G'GATC_C
Cuts at: 3247 3614 3247
Size: 367 5550
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2114 2414 2773 3057 3238
Size: 325 217 627 327 300 359 284 181
Cuts at: 3238 4693 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 359 327 325 300 284
217 181
BanII G_rGCy'C
Cuts at: 1391 2450 2538 2730 3236 1391
Size: 1059 88 192 506 4072
Fragments arranged by size:
4072 1059 506 192 88
BbeI G_GCGC'C
Cuts at: 2777 3061 2777
Size: 284 5633
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2206 3146 928
Size: 971 307 940 3699
Fragments arranged by size:
3699 971 940 307
BbvCI CC'TCA_GC
Cuts at: 2267 2267
Size: 5917
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4108 4142 1903
Size: 34 2171 34 3678
Fragments arranged by size:
3678 2171 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3809 5019 3809
Size: 1210 4707
BclI T'GATC_A
Cuts at: 1548 3159 1548
Size: 1611 4306
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2040 2445 4528
Size: 107 122 71 631 605 368 405 2083
Cuts at: 4528 136
Size: 1525
Fragments arranged by size:
2083 1525 631 605 405 368 122 107
71
BglII A'GATC_T
Cuts at: 5912 5912
Size: 5917
BlpI GC'TnA_GC
Cuts at: 2532 3334 2532
Size: 802 5115
Bme1580I G_kGCm'C
Cuts at: 1790 3978 5134 1790
Size: 2188 1156 2573
Fragments arranged by size:
2573 2188 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5917
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3648 4606 442
Size: 416 53 83 399 24 2231 958 1753
Fragments arranged by size:
2231 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5917
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2113 2113
Size: 5917
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2740 4577 1239
Size: 1501 1837 2579
Fragments arranged by size:
2579 1837 1501
Bpu10I CC'TnA_GC
Cuts at: 2267 2714 2267
Size: 447 5470
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2327 2903 4042 4907 5205 2327
Size: 576 1139 865 298 3039
Fragments arranged by size:
3039 1139 865 576 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2472 4580 882
Size: 617 973 2108 2219
Fragments arranged by size:
2219 2108 973 617
BsaAI yAC'GTr
Cuts at: 493 1204 3708 493
Size: 711 2504 2702
Fragments arranged by size:
2702 2504 711
BsaBI GATnn'nnATC
Cuts at: 5911 5911
Size: 5917
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2127 2774 2946 3058
Size: 53 83 186 936 594 647 172 112
Cuts at: 3058 3143 3724 4106 4741 275
Size: 85 581 382 635 1451
Fragments arranged by size:
1451 936 647 635 594 581 382 186
172 112 85 83 53
BsaWI w'CCGG_w
Cuts at: 2316 4350 4748 5022 5169 5580 2316
Size: 2034 398 274 147 411 2653
Fragments arranged by size:
2653 2034 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5692
Fragments arranged by size:
5692 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2159 2419 1186
Size: 973 260 4684
Fragments arranged by size:
4684 973 260
BseYI C'CCAG_C
Cuts at: 2025 2593 2878 3039 3045 3111 5120 5616 2025
Size: 568 285 161 6 66 2009 496 2326
Fragments arranged by size:
2326 2009 568 496 285 161 66 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2402 2750 1163
Size: 573 666 348 4330
Fragments arranged by size:
4330 666 573 348
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2088 2153 2392 2553 2904 3367
Size: 275 186 87 65 239 161 351 463
Cuts at: 3367 4128 5156 1540
Size: 761 1028 2301
Fragments arranged by size:
2301 1028 761 463 351 275 239 186
161 87 65
BsiHKAI G_wGCw'C
Cuts at: 2730 3978 4063 5134 2730
Size: 1248 85 1071 3513
Fragments arranged by size:
3513 1248 1071 85
BsmI GAATG_Cn'
Cuts at: 3428 3521 3428
Size: 93 5824
BsmBI CGTCTCn'nnnn_
Cuts at: 2345 2345
Size: 5917
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2450 2538 2730 3236 3978 4063 5134
Size: 399 660 88 192 506 742 85 1071
Cuts at: 5134 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 660 506 399 192 88
85
BspEI T'CCGG_A
Cuts at: 5580 5580
Size: 5917
BspHI T'CATG_A
Cuts at: 3806 5536 3806
Size: 1730 4187
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2152 2228 2285 2857 2881 2989 3272 844
Size: 1308 76 57 572 24 108 283 3489
Fragments arranged by size:
3489 1308 572 283 108 76 57 24
BsrBI CCG'CTC
Cuts at: 1394 1823 2149 2196 2472 3804 1394
Size: 429 326 47 276 1332 3507
Fragments arranged by size:
3507 1332 429 326 276 47
BsrDI GCAATG_nn'
Cuts at: 65 1248 4412 4586 65
Size: 1183 3164 174 1396
Fragments arranged by size:
3164 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2018 2280 2497 2683 2792 4561 4748
Size: 110 208 262 217 186 109 1769 187
Cuts at: 4748 1700
Size: 2869
Fragments arranged by size:
2869 1769 262 217 208 187 186 110
109
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5917
BssHII G'CGCG_C
Cuts at: 1925 2080 2527 2654 2824 2851 3085 1925
Size: 155 447 127 170 27 234 4757
Fragments arranged by size:
4757 447 234 170 155 127 27
BssSI C'ACGA_G
Cuts at: 3977 4989 3977
Size: 1012 4905
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2407 2524 794
Size: 1613 117 4187
Fragments arranged by size:
4187 1613 117
BstBI TT'CG_AA
Cuts at: 4721 4721
Size: 5917
BstEII G'GTnAC_C
Cuts at: 1184 2014 1184
Size: 830 5087
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 5917
BstYI r'GATC_y
Cuts at: 1972 3247 3614 3999 4016 5468 5554 5566 5912
Size: 1275 367 385 17 1452 86 12 346
Cuts at: 5912 1972
Size: 1977
Fragments arranged by size:
1977 1452 1275 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2010 2418 2643 2829 513
Size: 552 939 6 408 225 186 3601
Fragments arranged by size:
3601 939 552 408 225 186 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2911 487
Size: 683 56 1685 3493
Fragments arranged by size:
3493 1685 683 56
BtsI GCAGTG_nn'
Cuts at: 2469 3433 4227 4247 2469
Size: 964 794 20 4139
Fragments arranged by size:
4139 964 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5917
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5882
DraI TTT'AAA
Cuts at: 3221 3221
Size: 5917
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4924 809
Size: 507 3608 1802
Fragments arranged by size:
3608 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2085 2150
Size: 54 1425 50 275 18 177 78 65
Cuts at: 2150 2389 2421 2550 2688 2784 2814 2901 3364
Size: 239 32 129 138 96 30 87 463
Cuts at: 3364 4253 5882 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 463 275 239 177 138
129 96 87 78 65 54 50 43
32 30 18
EagI C'GGCC_G
Cuts at: 1537 1812 2085 2150 2389 2550 2901 3364 1537
Size: 275 273 65 239 161 351 463 4090
Fragments arranged by size:
4090 463 351 275 273 239 161 65
EarI CTCTTCn'nnn_
Cuts at: 1353 2489 2639 2735 2939 3847 1353
Size: 1136 150 96 204 908 3423
Fragments arranged by size:
3423 1136 908 204 150 96
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2246 2490 4504 4878 5024 387
Size: 1064 138 657 244 2014 374 146 1280
Fragments arranged by size:
2014 1280 1064 657 374 244 146 138
EcoICRI GAG'CTC
Cuts at: 2728 2728
Size: 5917
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2620 2740 3980 4577 5364 5632 1239
Size: 393 988 120 1240 597 787 268 1524
Fragments arranged by size:
1524 1240 988 787 597 393 268 120
EcoRI G'AATT_C
Cuts at: 3226 3604 3226
Size: 378 5539
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2368 2368
Size: 5917
FspI TGC'GCA
Cuts at: 4423 4423
Size: 5917
HaeII r_GCGC'y
Cuts at: 1678 2456 2705 2759 2777 3052 3061 5064 1678
Size: 778 249 54 18 275 9 2003 2531
Fragments arranged by size:
2531 2003 778 275 249 54 18 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2087 2119 4558 4590 4632
Size: 32 135 32 515 32 2439 32 42
Cuts at: 4632 4664 1373
Size: 32 2626
Fragments arranged by size:
2626 2439 515 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2896 3261 3507 669
Size: 988 281 958 365 246 3079
Fragments arranged by size:
3079 988 958 365 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5917
HpaI GTT'AAC
Cuts at: 3507 3507
Size: 5917
KasI G'GCGC_C
Cuts at: 2773 3057 2773
Size: 284 5633
KpnI G_GTAC'C
Cuts at: 3242 3242
Size: 5917
MfeI C'AATT_G
Cuts at: 3516 3516
Size: 5917
MluI A'CGCG_T
Cuts at: 5793 5793
Size: 5917
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2310 5031 5215 1305
Size: 1005 2721 184 2007
Fragments arranged by size:
2721 2007 1005 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 10
Size: 54 1425 343 177 3918
Fragments arranged by size:
3918 1425 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2208 3875 4234 4393 518
Size: 648 843 199 1667 359 159 2042
Fragments arranged by size:
2042 1667 843 648 359 199 159
MspA1I CmG'CkG
Cuts at: 1606 2521 3115 3333 4010 5158 5403 1606
Size: 915 594 218 677 1148 245 2120
Fragments arranged by size:
2120 1148 915 677 594 245 218
NaeI GCC'GGC
Cuts at: 2794 2794
Size: 5917
NarI GG'CG_CC
Cuts at: 2774 3058 2774
Size: 284 5633
NcoI C'CATG_G
Cuts at: 513 1065 2004 2010 513
Size: 552 939 6 4420
Fragments arranged by size:
4420 939 552 6
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5917
NgoMIV G'CCGG_C
Cuts at: 2792 2792
Size: 5917
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5917
NotI GC'GGCC_GC
Cuts at: 2389 3364 2389
Size: 975 4942
NspI r_CATG'y
Cuts at: 1132 1229 3172 3275 4820 1132
Size: 97 1943 103 1545 2229
Fragments arranged by size:
2229 1943 1545 103 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5917
PciI A'CATG_T
Cuts at: 4816 4816
Size: 5917
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5917
PfoI T'CCnGG_A
Cuts at: 2859 2859
Size: 5917
PmeI GTTT'AAAC
Cuts at: 3221 3221
Size: 5917
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3986 4018 5499 5531 3986
Size: 32 1481 32 4372
Fragments arranged by size:
4372 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5917
PsiI TTA'TAA
Cuts at: 3487 3487
Size: 5917
PstI C_TGCA'G
Cuts at: 830 2163 2892 2988 3269 830
Size: 1333 729 96 281 3478
Fragments arranged by size:
3478 1333 729 281 96
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5917
PvuII CAG'CTG
Cuts at: 2521 3115 2521
Size: 594 5323
SacI G_AGCT'C
Cuts at: 2730 2730
Size: 5917
SalI G'TCGA_C
Cuts at: 1936 2894 3259 1936
Size: 958 365 4594
Fragments arranged by size:
4594 958 365
SapI GCTCTTCn'nnn_
Cuts at: 2639 2735 2939 2639
Size: 96 204 5617
Fragments arranged by size:
5617 204 96
SbfI CC_TGCA'GG
Cuts at: 2892 2988 3269 2892
Size: 96 281 5540
Fragments arranged by size:
5540 281 96
ScaI AGT'ACT
Cuts at: 2333 4165 5877 2333
Size: 1832 1712 2373
Fragments arranged by size:
2373 1832 1712
SexAI A'CCwGG_T
Cuts at: 2694 2844 2694
Size: 150 5767
SfcI C'TryA_G
Cuts at: 826 1045 2159 2888 2984 3265 4400 5081 5272
Size: 219 1114 729 96 281 1135 681 191
Cuts at: 5272 826
Size: 1471
Fragments arranged by size:
1471 1135 1114 729 681 281 219 191
96
SfoI GGC'GCC
Cuts at: 2775 3059 2775
Size: 284 5633
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5917
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5917
SmaI CCC'GGG
Cuts at: 1872 2445 2490 3244 1872
Size: 573 45 754 4545
Fragments arranged by size:
4545 754 573 45
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2342 2918 4021 4922 5184 820
Size: 197 926 399 576 1103 901 262 1553
Fragments arranged by size:
1553 1103 926 901 576 399 262 197
SnaBI TAC'GTA
Cuts at: 493 3708 493
Size: 3215 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5917
SphI G_CATG'C
Cuts at: 1132 3275 1132
Size: 2143 3774
SspI AAT'ATT
Cuts at: 5 52 3841 5
Size: 47 3789 2081
Fragments arranged by size:
3789 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5917
StyI C'CwwG_G
Cuts at: 513 1065 2004 2010 3356 513
Size: 552 939 6 1346 3074
Fragments arranged by size:
3074 1346 939 552 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3944 3961 4114 4299 4744 325
Size: 1255 2364 17 153 185 445 1498
Fragments arranged by size:
2364 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2331 4163 5875
Size: 274 80 33 51 1443 354 1832 1712
Cuts at: 5875 96
Size: 138
Fragments arranged by size:
1832 1712 1443 354 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5877 5909 5877
Size: 32 5885
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5917
XbaI T'CTAG_A
Cuts at: 3253 3253
Size: 5917
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5917
XmaI C'CCGG_G
Cuts at: 1870 2443 2488 3242 1870
Size: 573 45 754 4545
Fragments arranged by size:
4545 754 573 45
XmnI GAAnn'nnTTC
Cuts at: 2351 4046 2351
Size: 1695 4222
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3725 276
Size: 53 83 186 936 2191 2468
Fragments arranged by size:
2468 2191 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AscI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AvrII BaeI BfrBI BsiWI BstZ17I Bsu36I
DraIII EcoNI EcoO109I EcoRV FseI FspAI NruI NsiI
PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII
SanDI SfiI SrfI SwaI XcmI