Restriction map for
pFN21AB9717
(Circular) (Six-base) MAPSORT of: pFN21AB9717.seq Check: 6495 from: 1 to: 5890
pFN21AB9717 5890 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3700 278
Size: 53 83 186 936 2164 2468
Fragments arranged by size:
2468 2164 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3233 1937
Size: 1296 4594
Acc65I G'GTAC_C
Cuts at: 3211 3211
Size: 5890
AclI AA'CG_TT
Cuts at: 1563 4017 4390 1563
Size: 2454 373 3063
Fragments arranged by size:
3063 2454 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3165 3953 5337 5605 1632
Size: 1533 788 1384 268 1917
Fragments arranged by size:
1917 1533 1384 788 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5693
AflIII A'CryG_T
Cuts at: 1203 2249 3070 4789 5766 1203
Size: 1046 821 1719 977 1327
Fragments arranged by size:
1719 1327 1046 977 821
AgeI A'CCGG_T
Cuts at: 4721 4721
Size: 5890
AhdI GACnn_n'nnGTC
Cuts at: 4619 4619
Size: 5890
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5890
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2477 2796 1184
Size: 1293 319 4278
Fragments arranged by size:
4278 1293 319
AlwNI CAG_nnn'CTG
Cuts at: 3633 5205 3633
Size: 1572 4318
ApaLI G'TGCA_C
Cuts at: 3947 5103 3947
Size: 1156 4734
ApoI r'AATT_y
Cuts at: 1493 3003 3199 3425 3577 5686 1493
Size: 1510 196 226 152 2109 1697
Fragments arranged by size:
2109 1697 1510 226 196 152
AseI AT'TA_AT
Cuts at: 160 1032 4444 160
Size: 872 3412 1606
Fragments arranged by size:
3412 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5890
AvaI C'yCGr_G
Cuts at: 1870 1943 3215 1870
Size: 73 1272 4545
Fragments arranged by size:
4545 1272 73
BamHI G'GATC_C
Cuts at: 3220 3587 3220
Size: 367 5523
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3211 4666 618
Size: 325 217 627 1424 1455 1842
Fragments arranged by size:
1842 1455 1424 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3209 1391
Size: 1818 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2115 2226 928
Size: 971 216 111 4592
Fragments arranged by size:
4592 971 216 111
BbvCI CC'TCA_GC
Cuts at: 2769 2769
Size: 5890
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4081 4115 1903
Size: 34 2144 34 3678
Fragments arranged by size:
3678 2144 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3782 4992 3782
Size: 1210 4680
BclI T'GATC_A
Cuts at: 1548 2293 1548
Size: 745 5145
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4501 136
Size: 107 122 71 631 605 2829 1525
Fragments arranged by size:
2829 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5885 5885
Size: 5890
BlpI GC'TnA_GC
Cuts at: 3307 3307
Size: 5890
Bme1580I G_kGCm'C
Cuts at: 1790 3951 5107 1790
Size: 2161 1156 2573
Fragments arranged by size:
2573 2161 1156
BmgBI CAC'GTC
Cuts at: 1318 2250 1318
Size: 932 4958
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3621 4579 442
Size: 416 53 83 399 24 2204 958 1753
Fragments arranged by size:
2204 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2730 1056
Size: 1674 4216
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4550 1239
Size: 3311 2579
Bpu10I CC'TnA_GC
Cuts at: 2450 2769 2450
Size: 319 5571
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2716 4015 4880 5178 2716
Size: 1299 865 298 3428
Fragments arranged by size:
3428 1299 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4553 882
Size: 617 3054 2219
Fragments arranged by size:
3054 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2658 3681 493
Size: 711 1454 1023 2702
Fragments arranged by size:
2702 1454 1023 711
BsaBI GATnn'nnATC
Cuts at: 2298 5884 2298
Size: 3586 2304
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3697 4079 4714 275
Size: 53 83 186 936 2164 382 635 1451
Fragments arranged by size:
2164 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4323 4721 4995 5142 5553 4323
Size: 398 274 147 411 4660
Fragments arranged by size:
4660 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5665
Fragments arranged by size:
5665 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2709 1186
Size: 1523 4367
BseYI C'CCAG_C
Cuts at: 5093 5589 5093
Size: 496 5394
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5317
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3340 4101 5129 1540
Size: 275 186 1339 761 1028 2301
Fragments arranged by size:
2301 1339 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2151 3951 4036 5107 2151
Size: 1800 85 1071 2934
Fragments arranged by size:
2934 1800 1071 85
BsmI GAATG_Cn'
Cuts at: 2747 3401 3494 2747
Size: 654 93 5143
Fragments arranged by size:
5143 654 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2151 3209 3951 4036 5107 1391
Size: 399 361 1058 742 85 1071 2174
Fragments arranged by size:
2174 1071 1058 742 399 361 85
BspEI T'CCGG_A
Cuts at: 5553 5553
Size: 5890
BspHI T'CATG_A
Cuts at: 3130 3779 5509 3130
Size: 649 1730 3511
Fragments arranged by size:
3511 1730 649
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2426 3245 844
Size: 1582 819 3489
Fragments arranged by size:
3489 1582 819
BsrBI CCG'CTC
Cuts at: 1394 1823 3777 1394
Size: 429 1954 3507
Fragments arranged by size:
3507 1954 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2778 4385 4559 65
Size: 1183 1530 1607 174 1396
Fragments arranged by size:
1607 1530 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4534 4721 1700
Size: 110 2724 187 2869
Fragments arranged by size:
2869 2724 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5890
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5890
BssSI C'ACGA_G
Cuts at: 2640 3950 4962 2640
Size: 1310 1012 3568
Fragments arranged by size:
3568 1310 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5890
BstBI TT'CG_AA
Cuts at: 4694 4694
Size: 5890
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5890
BstYI r'GATC_y
Cuts at: 1972 3220 3587 3972 3989 5441 5527 5539 5885
Size: 1248 367 385 17 1452 86 12 346
Cuts at: 5885 1972
Size: 1977
Fragments arranged by size:
1977 1452 1248 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2152 2422 513
Size: 552 939 148 270 3981
Fragments arranged by size:
3981 939 552 270 148
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2047 487
Size: 683 56 821 4330
Fragments arranged by size:
4330 821 683 56
BtsI GCAGTG_nn'
Cuts at: 3406 4200 4220 3406
Size: 794 20 5076
Fragments arranged by size:
5076 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5890
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5855
DraI TTT'AAA
Cuts at: 2232 2259 3194 2232
Size: 27 935 4928
Fragments arranged by size:
4928 935 27
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2493 4897 809
Size: 507 1177 2404 1802
Fragments arranged by size:
2404 1802 1177 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3337 4226 5855
Size: 54 1425 50 275 18 1507 889 1629
Cuts at: 5855 8
Size: 43
Fragments arranged by size:
1629 1507 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3337 1537
Size: 275 1525 4090
Fragments arranged by size:
4090 1525 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3820 1353
Size: 2467 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4477 4851 4997 387
Size: 1064 138 2888 374 146 1280
Fragments arranged by size:
2888 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3165 3953 4550 5337 5605 1239
Size: 393 1533 788 597 787 268 1524
Fragments arranged by size:
1533 1524 788 787 597 393 268
EcoRI G'AATT_C
Cuts at: 3199 3577 3199
Size: 378 5512
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2183 2183
Size: 5890
FspI TGC'GCA
Cuts at: 4396 4396
Size: 5890
HaeII r_GCGC'y
Cuts at: 1678 5037 1678
Size: 3359 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3160 3192 4531 4563 4605
Size: 32 135 32 1588 32 1339 32 42
Cuts at: 4605 4637 1373
Size: 32 2626
Fragments arranged by size:
2626 1588 1339 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3234 3480 669
Size: 988 281 1296 246 3079
Fragments arranged by size:
3079 1296 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5890
HpaI GTT'AAC
Cuts at: 3480 3480
Size: 5890
KpnI G_GTAC'C
Cuts at: 3215 3215
Size: 5890
MfeI C'AATT_G
Cuts at: 3489 3489
Size: 5890
MluI A'CGCG_T
Cuts at: 5766 5766
Size: 5890
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2247 5004 5188 1305
Size: 942 2757 184 2007
Fragments arranged by size:
2757 2007 942 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4068
Fragments arranged by size:
4068 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2187 2459 2832 3075 3848 4207 4366
Size: 648 1021 272 373 243 773 359 159
Cuts at: 4366 518
Size: 2042
Fragments arranged by size:
2042 1021 773 648 373 359 272 243
159
MspA1I CmG'CkG
Cuts at: 1606 3306 3983 5131 5376 1606
Size: 1700 677 1148 245 2120
Fragments arranged by size:
2120 1700 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2152 2422 513
Size: 552 939 148 270 3981
Fragments arranged by size:
3981 939 552 270 148
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5890
NheI G'CTAG_C
Cuts at: 1052 2726 1052
Size: 1674 4216
NotI GC'GGCC_GC
Cuts at: 3337 3337
Size: 5890
NspI r_CATG'y
Cuts at: 1132 1229 2482 3074 3248 4793 1132
Size: 97 1253 592 174 1545 2229
Fragments arranged by size:
2229 1545 1253 592 174 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5890
PciI A'CATG_T
Cuts at: 3070 4789 3070
Size: 1719 4171
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5890
PmeI GTTT'AAAC
Cuts at: 3194 3194
Size: 5890
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3959 3991 5472 5504 3959
Size: 32 1481 32 4345
Fragments arranged by size:
4345 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5890
PsiI TTA'TAA
Cuts at: 2442 3460 2442
Size: 1018 4872
PstI C_TGCA'G
Cuts at: 830 2614 3242 830
Size: 1784 628 3478
Fragments arranged by size:
3478 1784 628
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5890
SalI G'TCGA_C
Cuts at: 1936 3232 1936
Size: 1296 4594
SbfI CC_TGCA'GG
Cuts at: 3242 3242
Size: 5890
ScaI AGT'ACT
Cuts at: 2505 2897 4138 5850 2505
Size: 392 1241 1712 2545
Fragments arranged by size:
2545 1712 1241 392
SfcI C'TryA_G
Cuts at: 826 1045 2610 3238 4373 5054 5245 826
Size: 219 1565 628 1135 681 191 1471
Fragments arranged by size:
1565 1471 1135 681 628 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5890
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5890
SmaI CCC'GGG
Cuts at: 1872 3217 1872
Size: 1345 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2731 3994 4895 5157 820
Size: 197 926 788 1263 901 262 1553
Fragments arranged by size:
1553 1263 926 901 788 262 197
SnaBI TAC'GTA
Cuts at: 493 3681 493
Size: 3188 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5890
SphI G_CATG'C
Cuts at: 1132 3248 1132
Size: 2116 3774
SspI AAT'ATT
Cuts at: 5 52 2288 2548 3814 5
Size: 47 2236 260 1266 2081
Fragments arranged by size:
2236 2081 1266 260 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5890
StyI C'CwwG_G
Cuts at: 513 1065 2004 2152 2422 3329 513
Size: 552 939 148 270 907 3074
Fragments arranged by size:
3074 939 907 552 270 148
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3917 3934 4087 4272 4717 325
Size: 1255 2337 17 153 185 445 1498
Fragments arranged by size:
2337 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2252 2503 2632
Size: 274 80 33 51 1443 275 251 129
Cuts at: 2632 2895 4136 5848 96
Size: 263 1241 1712 138
Fragments arranged by size:
1712 1443 1241 275 274 263 251 138
129 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5850 5882 5850
Size: 32 5858
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5890
XbaI T'CTAG_A
Cuts at: 3226 3226
Size: 5890
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5890
XmaI C'CCGG_G
Cuts at: 1870 3215 1870
Size: 1345 4545
XmnI GAAnn'nnTTC
Cuts at: 2112 4019 2112
Size: 1907 3983
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3698 276
Size: 53 83 186 936 2164 2468
Fragments arranged by size:
2468 2164 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI