Restriction map for
pFN21AB9711
(Circular) (Six-base) MAPSORT of: pFN21AB9711.seq Check: 736 from: 1 to: 5806
pFN21AB9711 5806 bp
With 182 enzymes: SgfI *
January 30, 2014 17:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3096 3096
Size: 5806
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3616 278
Size: 53 83 186 936 2080 2468
Fragments arranged by size:
2468 2080 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3149 1937
Size: 1212 4594
Acc65I G'GTAC_C
Cuts at: 3127 3127
Size: 5806
AclI AA'CG_TT
Cuts at: 1563 3933 4306 1563
Size: 2370 373 3063
Fragments arranged by size:
3063 2370 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2075 2827 3869 5253 5521 1632
Size: 443 752 1042 1384 268 1917
Fragments arranged by size:
1917 1384 1042 752 443 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5609
AflIII A'CryG_T
Cuts at: 1203 4705 5682 1203
Size: 3502 977 1327
Fragments arranged by size:
3502 1327 977
AgeI A'CCGG_T
Cuts at: 4637 4637
Size: 5806
AhdI GACnn_n'nnGTC
Cuts at: 4535 4535
Size: 5806
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5806
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2792 2888 3008 1184
Size: 1608 96 120 3982
Fragments arranged by size:
3982 1608 120 96
AlwNI CAG_nnn'CTG
Cuts at: 2239 2527 2596 3549 5121 2239
Size: 288 69 953 1572 2924
Fragments arranged by size:
2924 1572 953 288 69
ApaI G_GGCC'C
Cuts at: 2351 2351
Size: 5806
ApaLI G'TGCA_C
Cuts at: 2502 3863 5019 2502
Size: 1361 1156 3289
Fragments arranged by size:
3289 1361 1156
ApoI r'AATT_y
Cuts at: 1493 3115 3341 3493 5602 1493
Size: 1622 226 152 2109 1697
Fragments arranged by size:
2109 1697 1622 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4360 160
Size: 872 3328 1606
Fragments arranged by size:
3328 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5806
AvaI C'yCGr_G
Cuts at: 1870 1943 2138 2651 3131 1870
Size: 73 195 513 480 4545
Fragments arranged by size:
4545 513 480 195 73
BamHI G'GATC_C
Cuts at: 2637 3136 3503 2637
Size: 499 367 4940
Fragments arranged by size:
4940 499 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2059 2546 2970 3127 4582
Size: 325 217 627 272 487 424 157 1455
Cuts at: 4582 618
Size: 1842
Fragments arranged by size:
1842 1455 627 487 424 325 272 217
157
BanII G_rGCy'C
Cuts at: 1391 2015 2285 2351 3125 1391
Size: 624 270 66 774 4072
Fragments arranged by size:
4072 774 624 270 66
BbeI G_GCGC'C
Cuts at: 2063 2063
Size: 5806
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4835
BbvCI CC'TCA_GC
Cuts at: 2028 2028
Size: 5806
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2119 2153 3997 4031 1903
Size: 34 182 34 1844 34 3678
Fragments arranged by size:
3678 1844 182 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3698 4908 3698
Size: 1210 4596
BclI T'GATC_A
Cuts at: 1548 2268 1548
Size: 720 5086
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2724 2754 4417
Size: 107 122 71 631 605 1052 30 1663
Cuts at: 4417 136
Size: 1525
Fragments arranged by size:
1663 1525 1052 631 605 122 107 71
30
BglII A'GATC_T
Cuts at: 5801 5801
Size: 5806
BlpI GC'TnA_GC
Cuts at: 3223 3223
Size: 5806
Bme1580I G_kGCm'C
Cuts at: 1790 2351 2506 2652 3867 5023 1790
Size: 561 155 146 1215 1156 2573
Fragments arranged by size:
2573 1215 1156 561 155 146
BmgBI CAC'GTC
Cuts at: 1318 2322 1318
Size: 1004 4802
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3537 4495 442
Size: 416 53 83 399 24 2120 958 1753
Fragments arranged by size:
2120 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5806
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2549 4466 1239
Size: 1310 1917 2579
Fragments arranged by size:
2579 1917 1310
Bpu10I CC'TnA_GC
Cuts at: 2028 2028
Size: 5806
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2027 3931 4796 5094 2027
Size: 1904 865 298 2739
Fragments arranged by size:
2739 1904 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4469 882
Size: 617 2970 2219
Fragments arranged by size:
2970 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2383 2507 2872 3597 493
Size: 711 1179 124 365 725 2702
Fragments arranged by size:
2702 1179 725 711 365 124
BsaBI GATnn'nnATC
Cuts at: 5800 5800
Size: 5806
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2060 3613 3995 4630
Size: 53 83 186 936 527 1553 382 635
Cuts at: 4630 275
Size: 1451
Fragments arranged by size:
1553 1451 936 635 527 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2475 4239 4637 4911 5058 5469 2475
Size: 1764 398 274 147 411 2812
Fragments arranged by size:
2812 1764 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5581
Fragments arranged by size:
5581 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2675 1186
Size: 1489 4317
BseYI C'CCAG_C
Cuts at: 2981 5009 5505 2981
Size: 2028 496 3282
Fragments arranged by size:
3282 2028 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2087 2252 2864 1163
Size: 573 351 165 612 4105
Fragments arranged by size:
4105 612 573 351 165
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2122 2362 2978 3256 4017 5045
Size: 275 186 121 240 616 278 761 1028
Cuts at: 5045 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 616 278 275 240 186
121
BsiHKAI G_wGCw'C
Cuts at: 2022 2043 2139 2285 2382 2506 3867 3952 5023
Size: 21 96 146 97 124 1361 85 1071
Cuts at: 5023 2022
Size: 2805
Fragments arranged by size:
2805 1361 1071 146 124 97 96 85
21
BsmI GAATG_Cn'
Cuts at: 3317 3410 3317
Size: 93 5713
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2015 2022 2043 2139 2285 2351 2382
Size: 399 225 7 21 96 146 66 31
Cuts at: 2382 2506 2652 3125 3867 3952 5023 1391
Size: 124 146 473 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 473 399 225 146 146
124 96 85 66 31 21 7
BspEI T'CCGG_A
Cuts at: 5469 5469
Size: 5806
BspHI T'CATG_A
Cuts at: 3695 5425 3695
Size: 1730 4076
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2784 3096 3161 844
Size: 1940 312 65 3489
Fragments arranged by size:
3489 1940 312 65
BsrBI CCG'CTC
Cuts at: 1394 1823 3693 1394
Size: 429 1870 3507
Fragments arranged by size:
3507 1870 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4301 4475 65
Size: 1183 3053 174 1396
Fragments arranged by size:
3053 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2357 2939 2973 3091 4450 4637 1700
Size: 110 547 582 34 118 1359 187 2869
Fragments arranged by size:
2869 1359 582 547 187 118 110 34
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5806
BssHII G'CGCG_C
Cuts at: 1925 2947 2964 1925
Size: 1022 17 4767
Fragments arranged by size:
4767 1022 17
BssSI C'ACGA_G
Cuts at: 3866 4878 3866
Size: 1012 4794
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2736 3088 794
Size: 1942 352 3512
Fragments arranged by size:
3512 1942 352
BstBI TT'CG_AA
Cuts at: 4610 4610
Size: 5806
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5806
BstXI CCAn_nnnn'nTGG
Cuts at: 3103 3103
Size: 5806
BstYI r'GATC_y
Cuts at: 1972 2637 3136 3503 3888 3905 5357 5443 5455
Size: 665 499 367 385 17 1452 86 12
Cuts at: 5455 5801 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 665 499 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2721 2752 3096 513
Size: 552 1656 31 344 3223
Fragments arranged by size:
3223 1656 552 344 31
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2257 2749 487
Size: 683 56 1031 492 3544
Fragments arranged by size:
3544 1031 683 492 56
BtsI GCAGTG_nn'
Cuts at: 3322 4116 4136 3322
Size: 794 20 4992
Fragments arranged by size:
4992 794 20
ClaI AT'CG_AT
Cuts at: 1554 2454 1554
Size: 900 4906
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5771
DraI TTT'AAA
Cuts at: 3110 3110
Size: 5806
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4813 809
Size: 507 3497 1802
Fragments arranged by size:
3497 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2076 2119 2359
Size: 54 1425 50 275 18 246 43 240
Cuts at: 2359 2937 2975 3093 3253 4142 5771 8
Size: 578 38 118 160 889 1629 43
Fragments arranged by size:
1629 1425 889 578 275 246 240 160
118 54 50 43 43 38 18
EagI C'GGCC_G
Cuts at: 1537 1812 2119 2359 2975 3253 1537
Size: 275 307 240 616 278 4090
Fragments arranged by size:
4090 616 307 278 275 240
EarI CTCTTCn'nnn_
Cuts at: 1353 2156 2954 3736 1353
Size: 803 798 782 3423
Fragments arranged by size:
3423 803 798 782
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2717 4393 4767 4913 387
Size: 1064 138 1128 1676 374 146 1280
Fragments arranged by size:
1676 1280 1128 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2283 2283
Size: 5806
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2075 2549 2827 3869 4466 5253 5521
Size: 393 443 474 278 1042 597 787 268
Cuts at: 5521 1239
Size: 1524
Fragments arranged by size:
1524 1042 787 597 474 443 393 278
268
EcoO109I rG'GnC_Cy
Cuts at: 2032 2299 2348 2032
Size: 267 49 5490
Fragments arranged by size:
5490 267 49
EcoRI G'AATT_C
Cuts at: 3115 3493 3115
Size: 378 5428
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2206 2206
Size: 5806
FseI GG_CCGG'CC
Cuts at: 2943 2943
Size: 5806
FspI TGC'GCA
Cuts at: 2792 2864 4312 2792
Size: 72 1448 4286
Fragments arranged by size:
4286 1448 72
FspAI rTGC'GCAy
Cuts at: 2792 2792
Size: 5806
HaeII r_GCGC'y
Cuts at: 1678 2063 2075 2896 2925 4953 1678
Size: 385 12 821 29 2028 2531
Fragments arranged by size:
2531 2028 821 385 29 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2260 2292 2727 2759 4447
Size: 32 135 32 688 32 435 32 1688
Cuts at: 4447 4479 4521 4553 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1688 688 435 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3150 3396 669
Size: 988 281 1212 246 3079
Fragments arranged by size:
3079 1212 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5806
HpaI GTT'AAC
Cuts at: 3396 3396
Size: 5806
KasI G'GCGC_C
Cuts at: 2059 2059
Size: 5806
KpnI G_GTAC'C
Cuts at: 3131 3131
Size: 5806
MfeI C'AATT_G
Cuts at: 3405 3405
Size: 5806
MluI A'CGCG_T
Cuts at: 5682 5682
Size: 5806
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2118 4920 5104 1305
Size: 813 2802 184 2007
Fragments arranged by size:
2802 2007 813 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3984
Fragments arranged by size:
3984 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2573 3764 4123 4282 518
Size: 648 1407 1191 359 159 2042
Fragments arranged by size:
2042 1407 1191 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2124 2723 2754 2935 3222 3899 5047 5292
Size: 518 599 31 181 287 677 1148 245
Cuts at: 5292 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 599 518 287 245 181
31
NaeI GCC'GGC
Cuts at: 2941 3093 2941
Size: 152 5654
NarI GG'CG_CC
Cuts at: 2060 2060
Size: 5806
NcoI C'CATG_G
Cuts at: 513 1065 3096 513
Size: 552 2031 3223
Fragments arranged by size:
3223 2031 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5806
NgoMIV G'CCGG_C
Cuts at: 2939 3091 2939
Size: 152 5654
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5806
NotI GC'GGCC_GC
Cuts at: 3253 3253
Size: 5806
NspI r_CATG'y
Cuts at: 1132 1229 2734 2791 3164 4709 1132
Size: 97 1505 57 373 1545 2229
Fragments arranged by size:
2229 1545 1505 373 97 57
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5806
PciI A'CATG_T
Cuts at: 4705 4705
Size: 5806
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2322 2803 1108
Size: 1214 481 4111
Fragments arranged by size:
4111 1214 481
PmeI GTTT'AAAC
Cuts at: 3110 3110
Size: 5806
PmlI CAC'GTG
Cuts at: 2383 2872 2383
Size: 489 5317
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3875 3907 5388 5420 3875
Size: 32 1481 32 4261
Fragments arranged by size:
4261 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5806
PsiI TTA'TAA
Cuts at: 3376 3376
Size: 5806
PspOMI G'GGCC_C
Cuts at: 2347 2347
Size: 5806
PstI C_TGCA'G
Cuts at: 830 2997 3158 830
Size: 2167 161 3478
Fragments arranged by size:
3478 2167 161
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5806
PvuII CAG'CTG
Cuts at: 2935 2935
Size: 5806
SacI G_AGCT'C
Cuts at: 2285 2285
Size: 5806
SacII CC_GC'GG
Cuts at: 2724 2755 2724
Size: 31 5775
SalI G'TCGA_C
Cuts at: 1936 3148 1936
Size: 1212 4594
SapI GCTCTTCn'nnn_
Cuts at: 2156 2954 2156
Size: 798 5008
SbfI CC_TGCA'GG
Cuts at: 3158 3158
Size: 5806
ScaI AGT'ACT
Cuts at: 4054 5766 4054
Size: 1712 4094
SfcI C'TryA_G
Cuts at: 826 1045 2993 3154 4289 4970 5161 826
Size: 219 1948 161 1135 681 191 1471
Fragments arranged by size:
1948 1471 1135 681 219 191 161
SfiI GGCCn_nnn'nGGCC
Cuts at: 2724 2724
Size: 5806
SfoI GGC'GCC
Cuts at: 2061 2061
Size: 5806
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5806
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5806
SmaI CCC'GGG
Cuts at: 1872 2653 3133 1872
Size: 781 480 4545
Fragments arranged by size:
4545 781 480
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2042 3910 4811 5073 820
Size: 197 926 99 1868 901 262 1553
Fragments arranged by size:
1868 1553 926 901 262 197 99
SnaBI TAC'GTA
Cuts at: 493 3597 493
Size: 3104 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5806
SphI G_CATG'C
Cuts at: 1132 2734 3164 1132
Size: 1602 430 3774
Fragments arranged by size:
3774 1602 430
SrfI GCCC'GGGC
Cuts at: 2653 2653
Size: 5806
SspI AAT'ATT
Cuts at: 5 52 3730 5
Size: 47 3678 2081
Fragments arranged by size:
3678 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5806
StyI C'CwwG_G
Cuts at: 513 1065 3096 3245 513
Size: 552 2031 149 3074
Fragments arranged by size:
3074 2031 552 149
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3833 3850 4003 4188 4633 325
Size: 1255 2253 17 153 185 445 1498
Fragments arranged by size:
2253 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4052 5764 96
Size: 274 80 33 51 1443 2075 1712 138
Fragments arranged by size:
2075 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2629 2661 5766 5798 2629
Size: 32 3105 32 2637
Fragments arranged by size:
3105 2637 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5806
XbaI T'CTAG_A
Cuts at: 3142 3142
Size: 5806
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5806
XmaI C'CCGG_G
Cuts at: 1870 2651 3131 1870
Size: 781 480 4545
Fragments arranged by size:
4545 781 480
XmnI GAAnn'nnTTC
Cuts at: 3935 3935
Size: 5806
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3614 276
Size: 53 83 186 936 2080 2468
Fragments arranged by size:
2468 2080 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FseI FspI FspAI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SacII SalI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV NruI NsiI
PacI PfoI PpuMI PspXI PsrI RsrII SanDI SexAI
SwaI XcmI