Restriction map for
pFN21AB9710
(Circular) (Six-base) MAPSORT of: pFN21AB9710.seq Check: 5058 from: 1 to: 5749
pFN21AB9710 5749 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2863 2863
Size: 5749
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3559 278
Size: 53 83 186 936 2023 2468
Fragments arranged by size:
2468 2023 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2124 2732 3092 1937
Size: 187 608 360 4594
Fragments arranged by size:
4594 608 360 187
Acc65I G'GTAC_C
Cuts at: 3070 3070
Size: 5749
AclI AA'CG_TT
Cuts at: 1563 3876 4249 1563
Size: 2313 373 3063
Fragments arranged by size:
3063 2313 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2053 3812 5196 5464 1632
Size: 421 1759 1384 268 1917
Fragments arranged by size:
1917 1759 1384 421 268
AfeI AGC'GCT
Cuts at: 2223 2834 2223
Size: 611 5138
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5552
AflIII A'CryG_T
Cuts at: 1203 4648 5625 1203
Size: 3445 977 1327
Fragments arranged by size:
3445 1327 977
AgeI A'CCGG_T
Cuts at: 4580 4580
Size: 5749
AhdI GACnn_n'nnGTC
Cuts at: 4478 4478
Size: 5749
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5749
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5749
AlwNI CAG_nnn'CTG
Cuts at: 2398 2782 3492 5064 2398
Size: 384 710 1572 3083
Fragments arranged by size:
3083 1572 710 384
ApaLI G'TGCA_C
Cuts at: 3806 4962 3806
Size: 1156 4593
ApoI r'AATT_y
Cuts at: 1493 3058 3284 3436 5545 1493
Size: 1565 226 152 2109 1697
Fragments arranged by size:
2109 1697 1565 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4303 160
Size: 872 3271 1606
Fragments arranged by size:
3271 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5749
AvaI C'yCGr_G
Cuts at: 1870 1943 2674 3074 1870
Size: 73 731 400 4545
Fragments arranged by size:
4545 731 400 73
BamHI G'GATC_C
Cuts at: 3079 3446 3079
Size: 367 5382
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2711 3070 4525 618
Size: 325 217 627 924 359 1455 1842
Fragments arranged by size:
1842 1455 924 627 359 325 217
BanII G_rGCy'C
Cuts at: 1391 2483 2955 3068 1391
Size: 1092 472 113 4072
Fragments arranged by size:
4072 1092 472 113
BbeI G_GCGC'C
Cuts at: 2715 2715
Size: 5749
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2577 2676 928
Size: 971 678 99 4001
Fragments arranged by size:
4001 971 678 99
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3940 3974 1903
Size: 34 2003 34 3678
Fragments arranged by size:
3678 2003 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3641 4851 3641
Size: 1210 4539
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5749
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2155 4360 136
Size: 107 122 71 631 605 483 2205 1525
Fragments arranged by size:
2205 1525 631 605 483 122 107 71
BglII A'GATC_T
Cuts at: 5744 5744
Size: 5749
BlpI GC'TnA_GC
Cuts at: 3166 3166
Size: 5749
Bme1580I G_kGCm'C
Cuts at: 1790 3810 4966 1790
Size: 2020 1156 2573
Fragments arranged by size:
2573 2020 1156
BmgBI CAC'GTC
Cuts at: 1318 2730 2995 1318
Size: 1412 265 4072
Fragments arranged by size:
4072 1412 265
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3480 4438 442
Size: 416 53 83 399 24 2063 958 1753
Fragments arranged by size:
2063 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5749
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2617 2617
Size: 5749
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2263 2627 2708 2804 4409 1239
Size: 1024 364 81 96 1605 2579
Fragments arranged by size:
2579 1605 1024 364 96 81
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3874 4739 5037 3874
Size: 865 298 4586
Fragments arranged by size:
4586 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2687 4412 882
Size: 617 1188 1725 2219
Fragments arranged by size:
2219 1725 1188 617
BsaAI yAC'GTr
Cuts at: 493 1204 3540 493
Size: 711 2336 2702
Fragments arranged by size:
2702 2336 711
BsaBI GATnn'nnATC
Cuts at: 2637 5743 2637
Size: 3106 2643
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2712 3556 3938 4573
Size: 53 83 186 936 1179 844 382 635
Cuts at: 4573 275
Size: 1451
Fragments arranged by size:
1451 1179 936 844 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4182 4580 4854 5001 5412 4182
Size: 398 274 147 411 4519
Fragments arranged by size:
4519 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5524
Fragments arranged by size:
5524 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2606 2705 1186
Size: 1420 99 4230
Fragments arranged by size:
4230 1420 99
BseYI C'CCAG_C
Cuts at: 2050 2105 2255 2352 4952 5448 2050
Size: 55 150 97 2600 496 2351
Fragments arranged by size:
2600 2351 496 150 97 55
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2534 2891 1163
Size: 573 798 357 4021
Fragments arranged by size:
4021 798 573 357
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2159 2505 3199 3960 4988 1540
Size: 275 186 158 346 694 761 1028 2301
Fragments arranged by size:
2301 1028 761 694 346 275 186 158
BsiHKAI G_wGCw'C
Cuts at: 3810 3895 4966 3810
Size: 85 1071 4593
Fragments arranged by size:
4593 1071 85
BsmI GAATG_Cn'
Cuts at: 3260 3353 3260
Size: 93 5656
BsmBI CGTCTCn'nnnn_
Cuts at: 2501 2501
Size: 5749
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2483 2955 3068 3810 3895 4966 1391
Size: 399 693 472 113 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 693 472 399 113 85
BspEI T'CCGG_A
Cuts at: 5412 5412
Size: 5749
BspHI T'CATG_A
Cuts at: 3638 5368 3638
Size: 1730 4019
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2863 2991 3104 844
Size: 2019 128 113 3489
Fragments arranged by size:
3489 2019 128 113
BsrBI CCG'CTC
Cuts at: 1394 1823 3636 1394
Size: 429 1813 3507
Fragments arranged by size:
3507 1813 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2935 4244 4418 65
Size: 1183 1687 1309 174 1396
Fragments arranged by size:
1687 1396 1309 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2129 2154 2158 2745 4393 4580 1700
Size: 110 319 25 4 587 1648 187 2869
Fragments arranged by size:
2869 1648 587 319 187 110 25 4
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5749
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5749
BssSI C'ACGA_G
Cuts at: 3809 4821 3809
Size: 1012 4737
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2398 2930 794
Size: 1604 532 3613
Fragments arranged by size:
3613 1604 532
BstBI TT'CG_AA
Cuts at: 4553 4553
Size: 5749
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5749
BstYI r'GATC_y
Cuts at: 1972 2250 3079 3446 3831 3848 5300 5386 5398
Size: 278 829 367 385 17 1452 86 12
Cuts at: 5398 5744 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 829 385 367 346 278 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2499 2715 513
Size: 552 1434 216 3547
Fragments arranged by size:
3547 1434 552 216
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2628 487
Size: 683 56 1402 3608
Fragments arranged by size:
3608 1402 683 56
BtsI GCAGTG_nn'
Cuts at: 2803 3265 4059 4079 2803
Size: 462 794 20 4473
Fragments arranged by size:
4473 794 462 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5749
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5714
DraI TTT'AAA
Cuts at: 3053 3053
Size: 5749
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4756 809
Size: 507 3440 1802
Fragments arranged by size:
3440 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2113 2156 2319
Size: 54 1425 50 275 18 283 43 163
Cuts at: 2319 2469 2502 2647 3196 4085 5714 8
Size: 150 33 145 549 889 1629 43
Fragments arranged by size:
1629 1425 889 549 283 275 163 150
145 54 50 43 43 33 18
EagI C'GGCC_G
Cuts at: 1537 1812 2156 2502 3196 1537
Size: 275 344 346 694 4090
Fragments arranged by size:
4090 694 346 344 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3679 1353
Size: 2326 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2441 2770 4336 4710 4856 387
Size: 1064 138 852 329 1566 374 146 1280
Fragments arranged by size:
1566 1280 1064 852 374 329 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2053 2263 2627 2708 2804 3812 4409
Size: 393 421 210 364 81 96 1008 597
Cuts at: 4409 5196 5464 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1008 787 597 421 393 364 268
210 96 81
EcoNI CCTnn'n_nnAGG
Cuts at: 2288 2288
Size: 5749
EcoRI G'AATT_C
Cuts at: 3058 3436 3058
Size: 378 5371
FspI TGC'GCA
Cuts at: 4255 4255
Size: 5749
HaeII r_GCGC'y
Cuts at: 1678 2047 2225 2303 2435 2715 2836 4896 1678
Size: 369 178 78 132 280 121 2060 2531
Fragments arranged by size:
2531 2060 369 280 178 132 121 78
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2612 2644 4390 4422 4464
Size: 32 135 32 1040 32 1746 32 42
Cuts at: 4464 4496 1373
Size: 32 2626
Fragments arranged by size:
2626 1746 1040 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3093 3339 669
Size: 988 281 1155 246 3079
Fragments arranged by size:
3079 1155 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5749
HpaI GTT'AAC
Cuts at: 3339 3339
Size: 5749
KasI G'GCGC_C
Cuts at: 2711 2711
Size: 5749
KpnI G_GTAC'C
Cuts at: 3074 3074
Size: 5749
MfeI C'AATT_G
Cuts at: 3348 3348
Size: 5749
MluI A'CGCG_T
Cuts at: 5625 5625
Size: 5749
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4863 5047 1305
Size: 3558 184 2007
Fragments arranged by size:
3558 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2321 2471 2649 10
Size: 54 1425 343 489 150 178 3110
Fragments arranged by size:
3110 1425 489 343 178 150 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2144 3707 4066 4225 518
Size: 648 978 1563 359 159 2042
Fragments arranged by size:
2042 1563 978 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2050 2372 2398 2501 2709 2717 2779 3165
Size: 444 322 26 103 208 8 62 386
Cuts at: 3165 3842 4990 5235 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 444 386 322 245 208
103 62 26 8
NarI GG'CG_CC
Cuts at: 2712 2712
Size: 5749
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5197
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5749
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5749
NotI GC'GGCC_GC
Cuts at: 3196 3196
Size: 5749
NspI r_CATG'y
Cuts at: 1132 1229 3107 4652 1132
Size: 97 1878 1545 2229
Fragments arranged by size:
2229 1878 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5749
PciI A'CATG_T
Cuts at: 4648 4648
Size: 5749
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2686 1108
Size: 1578 4171
PfoI T'CCnGG_A
Cuts at: 2957 2957
Size: 5749
PmeI GTTT'AAAC
Cuts at: 3053 3053
Size: 5749
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2783 2815 3818 3850 5331 5363 2783
Size: 32 1003 32 1481 32 3169
Fragments arranged by size:
3169 1481 1003 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5749
PsiI TTA'TAA
Cuts at: 3319 3319
Size: 5749
PstI C_TGCA'G
Cuts at: 830 2217 2439 3101 830
Size: 1387 222 662 3478
Fragments arranged by size:
3478 1387 662 222
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5749
PvuII CAG'CTG
Cuts at: 2779 2779
Size: 5749
SacII CC_GC'GG
Cuts at: 2502 2718 2502
Size: 216 5533
SalI G'TCGA_C
Cuts at: 1936 3091 1936
Size: 1155 4594
SbfI CC_TGCA'GG
Cuts at: 3101 3101
Size: 5749
ScaI AGT'ACT
Cuts at: 3997 5709 3997
Size: 1712 4037
SfcI C'TryA_G
Cuts at: 826 1045 2213 2435 2623 3097 4232 4913 5104
Size: 219 1168 222 188 474 1135 681 191
Cuts at: 5104 826
Size: 1471
Fragments arranged by size:
1471 1168 1135 681 474 222 219 191
188
SfoI GGC'GCC
Cuts at: 2713 2713
Size: 5749
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5749
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5749
SmaI CCC'GGG
Cuts at: 1872 2676 3076 1872
Size: 804 400 4545
Fragments arranged by size:
4545 804 400
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3853 4754 5016 820
Size: 197 926 1910 901 262 1553
Fragments arranged by size:
1910 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3540 493
Size: 3047 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5749
SphI G_CATG'C
Cuts at: 1132 3107 1132
Size: 1975 3774
SspI AAT'ATT
Cuts at: 5 52 3673 5
Size: 47 3621 2081
Fragments arranged by size:
3621 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5749
StyI C'CwwG_G
Cuts at: 513 1065 3188 513
Size: 552 2123 3074
Fragments arranged by size:
3074 2123 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2843 3776 3793 3946 4131 4576 325
Size: 1255 1263 933 17 153 185 445 1498
Fragments arranged by size:
1498 1263 1255 933 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3995 5707 96
Size: 274 80 33 51 1443 2018 1712 138
Fragments arranged by size:
2018 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5709 5741 5709
Size: 32 5717
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5749
XbaI T'CTAG_A
Cuts at: 3085 3085
Size: 5749
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5749
XmaI C'CCGG_G
Cuts at: 1870 2674 3074 1870
Size: 804 400 4545
Fragments arranged by size:
4545 804 400
XmnI GAAnn'nnTTC
Cuts at: 2798 3878 2798
Size: 1080 4669
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3557 276
Size: 53 83 186 936 2023 2468
Fragments arranged by size:
2468 2023 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PstI PvuI PvuII SacII SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbvCI BfrBI Bpu10I
BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV
FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI
PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SanDI
SapI SexAI SfiI SrfI SwaI XcmI