Restriction map for
pFN21AB9707
(Circular) (Six-base) MAPSORT of: pFN21AB9707.seq Check: 235 from: 1 to: 5731
pFN21AB9707 5731 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3541 278
Size: 53 83 186 936 2005 2468
Fragments arranged by size:
2468 2005 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3074 1937
Size: 1137 4594
Acc65I G'GTAC_C
Cuts at: 2628 3052 2628
Size: 424 5307
AclI AA'CG_TT
Cuts at: 1563 3858 4231 1563
Size: 2295 373 3063
Fragments arranged by size:
3063 2295 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3794 5178 5446 1632
Size: 2162 1384 268 1917
Fragments arranged by size:
2162 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5534
AflIII A'CryG_T
Cuts at: 1203 2952 4630 5607 1203
Size: 1749 1678 977 1327
Fragments arranged by size:
1749 1678 1327 977
AgeI A'CCGG_T
Cuts at: 4562 4562
Size: 5731
AhdI GACnn_n'nnGTC
Cuts at: 4460 4460
Size: 5731
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5731
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2164 1184
Size: 980 4751
AlwNI CAG_nnn'CTG
Cuts at: 2329 3474 5046 2329
Size: 1145 1572 3014
Fragments arranged by size:
3014 1572 1145
ApaI G_GGCC'C
Cuts at: 2336 2357 2484 2559 2567 2336
Size: 21 127 75 8 5500
Fragments arranged by size:
5500 127 75 21 8
ApaLI G'TGCA_C
Cuts at: 2290 3788 4944 2290
Size: 1498 1156 3077
Fragments arranged by size:
3077 1498 1156
ApoI r'AATT_y
Cuts at: 1493 3040 3266 3418 5527 1493
Size: 1547 226 152 2109 1697
Fragments arranged by size:
2109 1697 1547 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4285 160
Size: 872 3253 1606
Fragments arranged by size:
3253 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5731
AvaI C'yCGr_G
Cuts at: 1870 1943 2350 2590 3056 1870
Size: 73 407 240 466 4545
Fragments arranged by size:
4545 466 407 240 73
BamHI G'GATC_C
Cuts at: 3061 3428 3061
Size: 367 5364
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2315 2381 2609 2628 3052
Size: 325 217 627 528 66 228 19 424
Cuts at: 3052 4507 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 528 424 325 228 217
66 19
BanII G_rGCy'C
Cuts at: 1391 2204 2244 2336 2357 2484 2559 2567 2639
Size: 813 40 92 21 127 75 8 72
Cuts at: 2639 2669 3050 1391
Size: 30 381 4072
Fragments arranged by size:
4072 813 381 127 92 75 72 40
30 21 8
BbeI G_GCGC'C
Cuts at: 2319 2385 2319
Size: 66 5665
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4760
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3922 3956 1903
Size: 34 1985 34 3678
Fragments arranged by size:
3678 1985 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3623 4833 3623
Size: 1210 4521
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5731
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2690 4342 136
Size: 107 122 71 631 605 1018 1652 1525
Fragments arranged by size:
1652 1525 1018 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5726 5726
Size: 5731
BlpI GC'TnA_GC
Cuts at: 2638 2888 3148 2638
Size: 250 260 5221
Fragments arranged by size:
5221 260 250
Bme1580I G_kGCm'C
Cuts at: 1790 2294 2336 2357 2484 2559 2567 2979 3792
Size: 504 42 21 127 75 8 412 813
Cuts at: 3792 4948 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 813 504 412 127 75 42
21 8
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5731
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2029 2206 2509
Size: 416 53 83 399 24 612 177 303
Cuts at: 2509 3462 4420 442
Size: 953 958 1753
Fragments arranged by size:
1753 958 953 612 416 399 303 177
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5731
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2263 2440 2658 2263
Size: 177 218 5336
Fragments arranged by size:
5336 218 177
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2034 2768 4391 1239
Size: 795 734 1623 2579
Fragments arranged by size:
2579 1623 795 734
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3856 4721 5019 3856
Size: 865 298 4568
Fragments arranged by size:
4568 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4394 882
Size: 617 2895 2219
Fragments arranged by size:
2895 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2955 3522 493
Size: 711 1751 567 2702
Fragments arranged by size:
2702 1751 711 567
BsaBI GATnn'nnATC
Cuts at: 5725 5725
Size: 5731
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2316 2382 3538 3920
Size: 53 83 186 936 783 66 1156 382
Cuts at: 3920 4555 275
Size: 635 1451
Fragments arranged by size:
1451 1156 936 783 635 382 186 83
66 53
BsaWI w'CCGG_w
Cuts at: 2992 4164 4562 4836 4983 5394 2992
Size: 1172 398 274 147 411 3329
Fragments arranged by size:
3329 1172 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2186 2216 1178
Size: 30 165 30 783 30 4693
Fragments arranged by size:
4693 783 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5731
BseYI C'CCAG_C
Cuts at: 2463 2978 4934 5430 2463
Size: 515 1956 496 2764
Fragments arranged by size:
2764 1956 515 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2198 1163
Size: 573 462 4696
Fragments arranged by size:
4696 573 462
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3181 3942 4970 1540
Size: 275 186 1180 761 1028 2301
Fragments arranged by size:
2301 1180 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2294 2639 3792 3877 4948 2294
Size: 345 1153 85 1071 3077
Fragments arranged by size:
3077 1153 1071 345 85
BsmI GAATG_Cn'
Cuts at: 3242 3335 3242
Size: 93 5638
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2204 2244 2294 2336 2357 2484 2559
Size: 399 414 40 50 42 21 127 75
Cuts at: 2559 2567 2639 2669 2979 3050 3792 3877 4948
Size: 8 72 30 310 71 742 85 1071
Cuts at: 4948 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 414 399 310 127 85
75 72 71 50 42 40 30 21
8
BspEI T'CCGG_A
Cuts at: 2992 5394 2992
Size: 2402 3329
BspHI T'CATG_A
Cuts at: 3620 5350 3620
Size: 1730 4001
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3086 844
Size: 2242 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2313 2937 3618 1394
Size: 429 490 624 681 3507
Fragments arranged by size:
3507 681 624 490 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4226 4400 65
Size: 1183 2978 174 1396
Fragments arranged by size:
2978 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2369 4375 4562 1700
Size: 110 559 2006 187 2869
Fragments arranged by size:
2869 2006 559 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5731
BssHII G'CGCG_C
Cuts at: 1925 2434 1925
Size: 509 5222
BssSI C'ACGA_G
Cuts at: 2425 3791 4803 2425
Size: 1366 1012 3353
Fragments arranged by size:
3353 1366 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5731
BstBI TT'CG_AA
Cuts at: 4535 4535
Size: 5731
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5731
BstXI CCAn_nnnn'nTGG
Cuts at: 2833 2833
Size: 5731
BstYI r'GATC_y
Cuts at: 1972 3061 3428 3813 3830 5282 5368 5380 5726
Size: 1089 367 385 17 1452 86 12 346
Cuts at: 5726 1972
Size: 1977
Fragments arranged by size:
1977 1452 1089 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2228 2261 2385 2406 2559 513
Size: 552 939 224 33 124 21 153 3685
Fragments arranged by size:
3685 939 552 224 153 124 33 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4992
Fragments arranged by size:
4992 683 56
BtsI GCAGTG_nn'
Cuts at: 2512 3247 4041 4061 2512
Size: 735 794 20 4182
Fragments arranged by size:
4182 794 735 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5731
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5696
DraI TTT'AAA
Cuts at: 3035 3035
Size: 5731
DraIII CAC_nnn'GTG
Cuts at: 2427 2427
Size: 5731
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4738 809
Size: 507 3422 1802
Fragments arranged by size:
3422 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3178 4067 5696
Size: 54 1425 50 275 18 1348 889 1629
Cuts at: 5696 8
Size: 43
Fragments arranged by size:
1629 1425 1348 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3178 1537
Size: 275 1366 4090
Fragments arranged by size:
4090 1366 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2262 3661 1353
Size: 909 1399 3423
Fragments arranged by size:
3423 1399 909
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2252 3024 4318 4692 4838 387
Size: 1064 138 663 772 1294 374 146 1280
Fragments arranged by size:
1294 1280 1064 772 663 374 146 138
EcoICRI GAG'CTC
Cuts at: 2637 2637
Size: 5731
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2034 2768 3794 4391 5178 5446 1239
Size: 393 402 734 1026 597 787 268 1524
Fragments arranged by size:
1524 1026 787 734 597 402 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2333 2354 2480 2720 2896 2333
Size: 21 126 240 176 5168
Fragments arranged by size:
5168 240 176 126 21
EcoRI G'AATT_C
Cuts at: 3040 3418 3040
Size: 378 5353
FspI TGC'GCA
Cuts at: 2930 4237 2930
Size: 1307 4424
HaeII r_GCGC'y
Cuts at: 1678 2319 2385 4878 1678
Size: 641 66 2493 2531
Fragments arranged by size:
2531 2493 641 66
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2015 2047 2186 2218 4372
Size: 32 135 32 443 32 139 32 2154
Cuts at: 4372 4404 4446 4478 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2154 443 139 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3075 3321 669
Size: 988 281 1137 246 3079
Fragments arranged by size:
3079 1137 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2179 748
Size: 1431 4300
HpaI GTT'AAC
Cuts at: 3321 3321
Size: 5731
KasI G'GCGC_C
Cuts at: 2315 2381 2315
Size: 66 5665
KpnI G_GTAC'C
Cuts at: 2632 3056 2632
Size: 424 5307
MfeI C'AATT_G
Cuts at: 3330 3330
Size: 5731
MluI A'CGCG_T
Cuts at: 5607 5607
Size: 5731
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2655 2768 4845 5029 1305
Size: 1350 113 2077 184 2007
Fragments arranged by size:
2077 2007 1350 184 113
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3909
Fragments arranged by size:
3909 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2604 3689 4048 4207 518
Size: 648 1438 1085 359 159 2042
Fragments arranged by size:
2042 1438 1085 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2191 2387 2463 2561 2731 2778 2815 3147
Size: 585 196 76 98 170 47 37 332
Cuts at: 3147 3824 4972 5217 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 585 332 245 196 170
98 76 47 37
NaeI GCC'GGC
Cuts at: 2371 2371
Size: 5731
NarI GG'CG_CC
Cuts at: 2316 2382 2316
Size: 66 5665
NcoI C'CATG_G
Cuts at: 513 1065 2004 2228 513
Size: 552 939 224 4016
Fragments arranged by size:
4016 939 552 224
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5731
NgoMIV G'CCGG_C
Cuts at: 2369 2369
Size: 5731
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5731
NotI GC'GGCC_GC
Cuts at: 3178 3178
Size: 5731
NspI r_CATG'y
Cuts at: 1132 1229 2151 2603 3089 4634 1132
Size: 97 922 452 486 1545 2229
Fragments arranged by size:
2229 1545 922 486 452 97
PasI CC'CwG_GG
Cuts at: 1348 2329 1348
Size: 981 4750
PciI A'CATG_T
Cuts at: 4630 4630
Size: 5731
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2427 2547 2583 1108
Size: 1319 120 36 4256
Fragments arranged by size:
4256 1319 120 36
PfoI T'CCnGG_A
Cuts at: 2582 2745 2582
Size: 163 5568
PmeI GTTT'AAAC
Cuts at: 3035 3035
Size: 5731
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3800 3832 5313 5345 3800
Size: 32 1481 32 4186
Fragments arranged by size:
4186 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2720 2720
Size: 5731
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5731
PsiI TTA'TAA
Cuts at: 3301 3301
Size: 5731
PspOMI G'GGCC_C
Cuts at: 2332 2353 2480 2555 2563 2332
Size: 21 127 75 8 5500
Fragments arranged by size:
5500 127 75 21 8
PstI C_TGCA'G
Cuts at: 830 3083 830
Size: 2253 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5731
PvuII CAG'CTG
Cuts at: 2191 2463 2731 2778 2815 2191
Size: 272 268 47 37 5107
Fragments arranged by size:
5107 272 268 47 37
RsrII CG'GwC_CG
Cuts at: 2677 2677
Size: 5731
SacI G_AGCT'C
Cuts at: 2639 2639
Size: 5731
SacII CC_GC'GG
Cuts at: 2388 2562 2388
Size: 174 5557
SalI G'TCGA_C
Cuts at: 1936 3073 1936
Size: 1137 4594
SapI GCTCTTCn'nnn_
Cuts at: 2262 2262
Size: 5731
SbfI CC_TGCA'GG
Cuts at: 3083 3083
Size: 5731
ScaI AGT'ACT
Cuts at: 3979 5691 3979
Size: 1712 4019
SfcI C'TryA_G
Cuts at: 826 1045 3079 4214 4895 5086 826
Size: 219 2034 1135 681 191 1471
Fragments arranged by size:
2034 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2317 2383 2317
Size: 66 5665
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5731
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5731
SmaI CCC'GGG
Cuts at: 1872 2352 2592 3058 1872
Size: 480 240 466 4545
Fragments arranged by size:
4545 480 466 240
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3835 4736 4998 820
Size: 197 926 1892 901 262 1553
Fragments arranged by size:
1892 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3522 493
Size: 3029 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5731
SphI G_CATG'C
Cuts at: 1132 2603 3089 1132
Size: 1471 486 3774
Fragments arranged by size:
3774 1471 486
SrfI GCCC'GGGC
Cuts at: 2352 2352
Size: 5731
SspI AAT'ATT
Cuts at: 5 52 3655 5
Size: 47 3603 2081
Fragments arranged by size:
3603 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5731
StyI C'CwwG_G
Cuts at: 513 1065 2004 2116 2228 3170 513
Size: 552 939 112 112 942 3074
Fragments arranged by size:
3074 942 939 552 112 112
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2232 3758 3775 3928 4113 4558 325
Size: 1255 652 1526 17 153 185 445 1498
Fragments arranged by size:
1526 1498 1255 652 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2949 3977 5689
Size: 274 80 33 51 1443 972 1028 1712
Cuts at: 5689 96
Size: 138
Fragments arranged by size:
1712 1443 1028 972 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5691 5723 5691
Size: 32 5699
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5731
XbaI T'CTAG_A
Cuts at: 3067 3067
Size: 5731
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2193 2226 2193
Size: 33 5698
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5731
XmaI C'CCGG_G
Cuts at: 1870 2350 2590 3056 1870
Size: 480 240 466 4545
Fragments arranged by size:
4545 480 466 240
XmnI GAAnn'nnTTC
Cuts at: 3860 3860
Size: 5731
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3539 276
Size: 53 83 186 936 2005 2468
Fragments arranged by size:
2468 2005 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FalI
FseI FspAI NruI NsiI PacI PmlI PspXI PsrI
SanDI SexAI SfiI SwaI