Restriction map for
pFN21AB9705
(Circular) (Six-base) MAPSORT of: pFN21AB9705.seq Check: 8896 from: 1 to: 5710
pFN21AB9705 5710 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3520 278
Size: 53 83 186 936 1984 2468
Fragments arranged by size:
2468 1984 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2796 3053 1937
Size: 859 257 4594
Fragments arranged by size:
4594 859 257
Acc65I G'GTAC_C
Cuts at: 2664 3031 2664
Size: 367 5343
AclI AA'CG_TT
Cuts at: 1563 3837 4210 1563
Size: 2274 373 3063
Fragments arranged by size:
3063 2274 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3773 5157 5425 1632
Size: 2141 1384 268 1917
Fragments arranged by size:
2141 1917 1384 268
AfeI AGC'GCT
Cuts at: 2344 2352 2344
Size: 8 5702
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5513
AflIII A'CryG_T
Cuts at: 1203 2487 2787 4609 5586 1203
Size: 1284 300 1822 977 1327
Fragments arranged by size:
1822 1327 1284 977 300
AgeI A'CCGG_T
Cuts at: 4541 4541
Size: 5710
AhdI GACnn_n'nnGTC
Cuts at: 2608 4439 2608
Size: 1831 3879
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5710
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5710
AlwNI CAG_nnn'CTG
Cuts at: 3453 5025 3453
Size: 1572 4138
ApaLI G'TGCA_C
Cuts at: 3767 4923 3767
Size: 1156 4554
ApoI r'AATT_y
Cuts at: 1493 2876 3019 3245 3397 5506 1493
Size: 1383 143 226 152 2109 1697
Fragments arranged by size:
2109 1697 1383 226 152 143
AscI GG'CGCG_CC
Cuts at: 2270 2270
Size: 5710
AseI AT'TA_AT
Cuts at: 160 1032 4264 160
Size: 872 3232 1606
Fragments arranged by size:
3232 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5710
AvaI C'yCGr_G
Cuts at: 1870 1943 2210 2338 3035 1870
Size: 73 267 128 697 4545
Fragments arranged by size:
4545 697 267 128 73
BamHI G'GATC_C
Cuts at: 3040 3407 3040
Size: 367 5343
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2033 2042 2527 2664 3031
Size: 325 217 627 246 9 485 137 367
Cuts at: 3031 4486 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 485 367 325 246 217
137 9
BanII G_rGCy'C
Cuts at: 1391 2234 2576 3029 1391
Size: 843 342 453 4072
Fragments arranged by size:
4072 843 453 342
BbeI G_GCGC'C
Cuts at: 2037 2046 2037
Size: 9 5701
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4739
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3901 3935 1903
Size: 34 1964 34 3678
Fragments arranged by size:
3678 1964 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2560 3602 4812 2560
Size: 1042 1210 3458
Fragments arranged by size:
3458 1210 1042
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5710
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4321 136
Size: 107 122 71 631 605 2649 1525
Fragments arranged by size:
2649 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5705 5705
Size: 5710
BlpI GC'TnA_GC
Cuts at: 3127 3127
Size: 5710
Bme1580I G_kGCm'C
Cuts at: 1790 2303 2592 3771 4927 1790
Size: 513 289 1179 1156 2573
Fragments arranged by size:
2573 1179 1156 513 289
BmgBI CAC'GTC
Cuts at: 1318 2488 1318
Size: 1170 4540
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3441 4399 442
Size: 416 53 83 399 24 2024 958 1753
Fragments arranged by size:
2024 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5710
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2331 2873 4370 1239
Size: 1092 542 1497 2579
Fragments arranged by size:
2579 1497 1092 542
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2417 3835 4700 4998 2417
Size: 1418 865 298 3129
Fragments arranged by size:
3129 1418 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4373 882
Size: 617 2874 2219
Fragments arranged by size:
2874 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2587 3501 493
Size: 711 1383 914 2702
Fragments arranged by size:
2702 1383 914 711
BsaBI GATnn'nnATC
Cuts at: 5704 5704
Size: 5710
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2034 2043 2087 3517
Size: 53 83 186 936 501 9 44 1430
Cuts at: 3517 3899 4534 275
Size: 382 635 1451
Fragments arranged by size:
1451 1430 936 635 501 382 186 83
53 44 9
BsaWI w'CCGG_w
Cuts at: 4143 4541 4815 4962 5373 4143
Size: 398 274 147 411 4480
Fragments arranged by size:
4480 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2846 2876 1178
Size: 30 165 30 1443 30 4012
Fragments arranged by size:
4012 1443 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2050 2053 1186
Size: 864 3 4843
Fragments arranged by size:
4843 864 3
BseYI C'CCAG_C
Cuts at: 2287 2302 2353 2455 4913 5409 2287
Size: 15 51 102 2458 496 2588
Fragments arranged by size:
2588 2458 496 102 51 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2695 1163
Size: 573 959 4178
Fragments arranged by size:
4178 959 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2325 3160 3921 4949 1540
Size: 275 186 324 835 761 1028 2301
Fragments arranged by size:
2301 1028 835 761 324 275 186
BsiHKAI G_wGCw'C
Cuts at: 3771 3856 4927 3771
Size: 85 1071 4554
Fragments arranged by size:
4554 1071 85
BsmI GAATG_Cn'
Cuts at: 2137 2431 3221 3314 2137
Size: 294 790 93 4533
Fragments arranged by size:
4533 790 294 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2234 2303 2576 2592 3029 3771 3856
Size: 399 444 69 273 16 437 742 85
Cuts at: 3856 4927 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 444 437 399 273 85
69 16
BspEI T'CCGG_A
Cuts at: 5373 5373
Size: 5710
BspHI T'CATG_A
Cuts at: 3599 5329 3599
Size: 1730 3980
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3065 844
Size: 2221 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3597 1394
Size: 429 1774 3507
Fragments arranged by size:
3507 1774 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4205 4379 65
Size: 1183 2957 174 1396
Fragments arranged by size:
2957 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2036 2515 4354 4541 1700
Size: 110 226 479 1839 187 2869
Fragments arranged by size:
2869 1839 479 226 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5710
BssHII G'CGCG_C
Cuts at: 1925 2270 1925
Size: 345 5365
BssSI C'ACGA_G
Cuts at: 2639 3770 4782 2639
Size: 1131 1012 3567
Fragments arranged by size:
3567 1131 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5710
BstBI TT'CG_AA
Cuts at: 4514 4514
Size: 5710
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5710
BstYI r'GATC_y
Cuts at: 1972 3040 3407 3792 3809 5261 5347 5359 5705
Size: 1068 367 385 17 1452 86 12 346
Cuts at: 5705 1972
Size: 1977
Fragments arranged by size:
1977 1452 1068 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2101 2469 2655 513
Size: 552 939 97 368 186 3568
Fragments arranged by size:
3568 939 552 368 186 97
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2424 487
Size: 683 56 1198 3773
Fragments arranged by size:
3773 1198 683 56
BtsI GCAGTG_nn'
Cuts at: 3226 4020 4040 3226
Size: 794 20 4896
Fragments arranged by size:
4896 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5710
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5675
DraI TTT'AAA
Cuts at: 3014 3014
Size: 5710
DraIII CAC_nnn'GTG
Cuts at: 2410 2410
Size: 5710
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2792 4717 809
Size: 507 1476 1925 1802
Fragments arranged by size:
1925 1802 1476 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2322 2517 3157
Size: 54 1425 50 275 18 492 195 640
Cuts at: 3157 4046 5675 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 640 492 275 195 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2322 3157 1537
Size: 275 510 835 4090
Fragments arranged by size:
4090 835 510 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2059 2239 2257 2797 2810 2956 3640 1353
Size: 706 180 18 540 13 146 684 3423
Fragments arranged by size:
3423 706 684 540 180 146 18 13
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4297 4671 4817 387
Size: 1064 138 2708 374 146 1280
Fragments arranged by size:
2708 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2331 2873 3773 4370 5157 5425 1239
Size: 393 699 542 900 597 787 268 1524
Fragments arranged by size:
1524 900 787 699 597 542 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2214 2733 2214
Size: 519 5191
EcoRI G'AATT_C
Cuts at: 3019 3397 3019
Size: 378 5332
FspI TGC'GCA
Cuts at: 4216 4216
Size: 5710
HaeII r_GCGC'y
Cuts at: 1678 2037 2046 2336 2346 2354 4857 1678
Size: 359 9 290 10 8 2503 2531
Fragments arranged by size:
2531 2503 359 290 10 9 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2594 2626 2990 3022 4351
Size: 32 135 32 1022 32 364 32 1329
Cuts at: 4351 4383 4425 4457 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1329 1022 364 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3054 3300 669
Size: 988 281 1116 246 3079
Fragments arranged by size:
3079 1116 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5710
HpaI GTT'AAC
Cuts at: 3300 3300
Size: 5710
KasI G'GCGC_C
Cuts at: 2033 2042 2033
Size: 9 5701
KpnI G_GTAC'C
Cuts at: 2668 3035 2668
Size: 367 5343
MfeI C'AATT_G
Cuts at: 3309 3309
Size: 5710
MluI A'CGCG_T
Cuts at: 5586 5586
Size: 5710
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4824 5008 1305
Size: 3519 184 2007
Fragments arranged by size:
3519 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3888
Fragments arranged by size:
3888 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2486 2654 3668 4027 4186 518
Size: 648 1320 168 1014 359 159 2042
Fragments arranged by size:
2042 1320 1014 648 359 168 159
MspA1I CmG'CkG
Cuts at: 1606 2103 2165 2186 2321 2327 2391 3126 3803
Size: 497 62 21 135 6 64 735 677
Cuts at: 3803 4951 5196 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 735 677 497 245 135 64
62 21 6
NarI GG'CG_CC
Cuts at: 2034 2043 2034
Size: 9 5701
NcoI C'CATG_G
Cuts at: 513 1065 2004 2655 513
Size: 552 939 651 3568
Fragments arranged by size:
3568 939 651 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5710
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5710
NotI GC'GGCC_GC
Cuts at: 2322 3157 2322
Size: 835 4875
NspI r_CATG'y
Cuts at: 1132 1229 2791 3068 4613 1132
Size: 97 1562 277 1545 2229
Fragments arranged by size:
2229 1562 1545 277 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5710
PciI A'CATG_T
Cuts at: 2787 4609 2787
Size: 1822 3888
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5710
PfoI T'CCnGG_A
Cuts at: 2672 2672
Size: 5710
PmeI GTTT'AAAC
Cuts at: 3014 3014
Size: 5710
PmlI CAC'GTG
Cuts at: 2587 2587
Size: 5710
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3779 3811 5292 5324 3779
Size: 32 1481 32 4165
Fragments arranged by size:
4165 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2214 2733 2214
Size: 519 5191
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5710
PsiI TTA'TAA
Cuts at: 3280 3280
Size: 5710
PstI C_TGCA'G
Cuts at: 830 2320 2332 2841 3062 830
Size: 1490 12 509 221 3478
Fragments arranged by size:
3478 1490 509 221 12
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5710
PvuII CAG'CTG
Cuts at: 2391 2391
Size: 5710
SacII CC_GC'GG
Cuts at: 2104 2104
Size: 5710
SalI G'TCGA_C
Cuts at: 1936 3052 1936
Size: 1116 4594
SanDI GG'GwC_CC
Cuts at: 2733 2733
Size: 5710
SapI GCTCTTCn'nnn_
Cuts at: 2239 2239
Size: 5710
SbfI CC_TGCA'GG
Cuts at: 3062 3062
Size: 5710
ScaI AGT'ACT
Cuts at: 3958 5670 3958
Size: 1712 3998
SfcI C'TryA_G
Cuts at: 826 1045 2316 2328 2386 2837 3058 4193 4874
Size: 219 1271 12 58 451 221 1135 681
Cuts at: 4874 5065 826
Size: 191 1471
Fragments arranged by size:
1471 1271 1135 681 451 221 219 191
58 12
SfoI GGC'GCC
Cuts at: 2035 2044 2035
Size: 9 5701
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5710
SgrAI Cr'CCGG_yG
Cuts at: 1700 2036 1700
Size: 336 5374
SmaI CCC'GGG
Cuts at: 1872 2212 3037 1872
Size: 340 825 4545
Fragments arranged by size:
4545 825 340
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2432 3814 4715 4977 820
Size: 197 926 489 1382 901 262 1553
Fragments arranged by size:
1553 1382 926 901 489 262 197
SnaBI TAC'GTA
Cuts at: 493 3501 493
Size: 3008 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5710
SphI G_CATG'C
Cuts at: 1132 3068 1132
Size: 1936 3774
SspI AAT'ATT
Cuts at: 5 52 3634 5
Size: 47 3582 2081
Fragments arranged by size:
3582 2081 47
StuI AGG'CCT
Cuts at: 1517 2496 2645 1517
Size: 979 149 4582
Fragments arranged by size:
4582 979 149
StyI C'CwwG_G
Cuts at: 513 1065 2004 2541 2655 2715 3149 513
Size: 552 939 537 114 60 434 3074
Fragments arranged by size:
3074 939 552 537 434 114 60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2323 3737 3754 3907 4092 4537 325
Size: 1255 743 1414 17 153 185 445 1498
Fragments arranged by size:
1498 1414 1255 743 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2478 2706 3956
Size: 274 80 33 51 1443 501 228 1250
Cuts at: 3956 5668 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1250 501 274 228 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5670 5702 5670
Size: 32 5678
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5710
XbaI T'CTAG_A
Cuts at: 3046 3046
Size: 5710
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5710
XmaI C'CCGG_G
Cuts at: 1870 2210 3035 1870
Size: 340 825 4545
Fragments arranged by size:
4545 825 340
XmnI GAAnn'nnTTC
Cuts at: 3839 3839
Size: 5710
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3518 276
Size: 53 83 186 936 1984 2468
Fragments arranged by size:
2468 1984 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AscI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI ApaI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI
EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI
PacI PspOMI PspXI PsrI RsrII SacI SexAI SfiI
SrfI SwaI XcmI