Restriction map for
pFN21AB9704
(Circular) (Six-base) MAPSORT of: pFN21AB9704.seq Check: 8580 from: 1 to: 5701
pFN21AB9704 5701 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2266 2266
Size: 5701
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3511 278
Size: 53 83 186 936 1975 2468
Fragments arranged by size:
2468 1975 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3044 1937
Size: 1107 4594
Acc65I G'GTAC_C
Cuts at: 2676 3022 2676
Size: 346 5355
AclI AA'CG_TT
Cuts at: 1563 3828 4201 1563
Size: 2265 373 3063
Fragments arranged by size:
3063 2265 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3764 5148 5416 1632
Size: 2132 1384 268 1917
Fragments arranged by size:
2132 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5504
AflIII A'CryG_T
Cuts at: 1203 4600 5577 1203
Size: 3397 977 1327
Fragments arranged by size:
3397 1327 977
AgeI A'CCGG_T
Cuts at: 4532 4532
Size: 5701
AhdI GACnn_n'nnGTC
Cuts at: 4430 4430
Size: 5701
AleI CACnn'nnGTG
Cuts at: 1166 2921 1166
Size: 1755 3946
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5701
AlwNI CAG_nnn'CTG
Cuts at: 2289 3444 5016 2289
Size: 1155 1572 2974
Fragments arranged by size:
2974 1572 1155
ApaI G_GGCC'C
Cuts at: 2221 2221
Size: 5701
ApaLI G'TGCA_C
Cuts at: 3758 4914 3758
Size: 1156 4545
ApoI r'AATT_y
Cuts at: 1493 3010 3236 3388 5497 1493
Size: 1517 226 152 2109 1697
Fragments arranged by size:
2109 1697 1517 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4255 160
Size: 872 3223 1606
Fragments arranged by size:
3223 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5701
AvaI C'yCGr_G
Cuts at: 1870 1943 2360 2513 3026 1870
Size: 73 417 153 513 4545
Fragments arranged by size:
4545 513 417 153 73
BamHI G'GATC_C
Cuts at: 3031 3398 3031
Size: 367 5334
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2676 2954 3022 4477 618
Size: 325 217 627 889 278 68 1455 1842
Fragments arranged by size:
1842 1455 889 627 325 278 217 68
BanII G_rGCy'C
Cuts at: 1391 2221 2862 3020 1391
Size: 830 641 158 4072
Fragments arranged by size:
4072 830 641 158
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2636 928
Size: 971 737 3993
Fragments arranged by size:
3993 971 737
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2824 2858 3892 3926 1903
Size: 34 887 34 1034 34 3678
Fragments arranged by size:
3678 1034 887 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3593 4803 3593
Size: 1210 4491
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5701
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2363 2559 2798
Size: 107 122 71 631 605 691 196 239
Cuts at: 2798 4312 136
Size: 1514 1525
Fragments arranged by size:
1525 1514 691 631 605 239 196 122
107 71
BglII A'GATC_T
Cuts at: 5696 5696
Size: 5701
BlpI GC'TnA_GC
Cuts at: 3118 3118
Size: 5701
Bme1580I G_kGCm'C
Cuts at: 1790 2221 3762 4918 1790
Size: 431 1541 1156 2573
Fragments arranged by size:
2573 1541 1156 431
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5701
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3432 4390 442
Size: 416 53 83 399 24 2015 958 1753
Fragments arranged by size:
2015 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5701
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2197 2321 2798 2962 4361 1239
Size: 958 124 477 164 1399 2579
Fragments arranged by size:
2579 1399 958 477 164 124
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2480 3826 4691 4989 2480
Size: 1346 865 298 3192
Fragments arranged by size:
3192 1346 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4364 882
Size: 617 2865 2219
Fragments arranged by size:
2865 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3492 493
Size: 711 2288 2702
Fragments arranged by size:
2702 2288 711
BsaBI GATnn'nnATC
Cuts at: 5695 5695
Size: 5701
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3508 3890 4525 275
Size: 53 83 186 936 1975 382 635 1451
Fragments arranged by size:
1975 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4134 4532 4806 4953 5364 4134
Size: 398 274 147 411 4471
Fragments arranged by size:
4471 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2063 2093 1178
Size: 30 165 30 660 30 4786
Fragments arranged by size:
4786 660 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2318 1186
Size: 1132 4569
BseYI C'CCAG_C
Cuts at: 2326 2702 4904 5400 2326
Size: 376 2202 496 2627
Fragments arranged by size:
2627 2202 496 376
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5128
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2551 2788 3151 3912 4940 1540
Size: 275 186 550 237 363 761 1028 2301
Fragments arranged by size:
2301 1028 761 550 363 275 237 186
BsiHKAI G_wGCw'C
Cuts at: 3762 3847 4918 3762
Size: 85 1071 4545
Fragments arranged by size:
4545 1071 85
BsmI GAATG_Cn'
Cuts at: 3212 3305 3212
Size: 93 5608
BsmBI CGTCTCn'nnnn_
Cuts at: 2288 2300 2288
Size: 12 5689
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2221 2862 3020 3762 3847 4918 1391
Size: 399 431 641 158 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 641 431 399 158 85
BspEI T'CCGG_A
Cuts at: 5364 5364
Size: 5701
BspHI T'CATG_A
Cuts at: 3590 5320 3590
Size: 1730 3971
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2266 3056 844
Size: 1422 790 3489
Fragments arranged by size:
3489 1422 790
BsrBI CCG'CTC
Cuts at: 1394 1823 2016 2079 2323 2650 2838 3588 1394
Size: 429 193 63 244 327 188 750 3507
Fragments arranged by size:
3507 750 429 327 244 193 188 63
BsrDI GCAATG_nn'
Cuts at: 65 1248 2635 4196 4370 65
Size: 1183 1387 1561 174 1396
Fragments arranged by size:
1561 1396 1387 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2732 2772 2951 4345 4532 1700
Size: 110 922 40 179 1394 187 2869
Fragments arranged by size:
2869 1394 922 187 179 110 40
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5701
BssHII G'CGCG_C
Cuts at: 1925 2401 2403 2405 2407 2941 1925
Size: 476 2 2 2 534 4685
Fragments arranged by size:
4685 534 476 2 2 2
BssSI C'ACGA_G
Cuts at: 2387 3761 4773 2387
Size: 1374 1012 3315
Fragments arranged by size:
3315 1374 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2712 794
Size: 1918 3783
BstBI TT'CG_AA
Cuts at: 4505 4505
Size: 5701
BstEII G'GTnAC_C
Cuts at: 1184 2971 1184
Size: 1787 3914
BstXI CCAn_nnnn'nTGG
Cuts at: 2089 2828 2089
Size: 739 4962
BstYI r'GATC_y
Cuts at: 1972 3031 3398 3783 3800 5252 5338 5350 5696
Size: 1059 367 385 17 1452 86 12 346
Cuts at: 5696 1972
Size: 1977
Fragments arranged by size:
1977 1452 1059 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2352 2412 2793 2898 513
Size: 552 939 348 60 381 105 3316
Fragments arranged by size:
3316 939 552 381 348 105 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4962
Fragments arranged by size:
4962 683 56
BtsI GCAGTG_nn'
Cuts at: 3217 4011 4031 3217
Size: 794 20 4887
Fragments arranged by size:
4887 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5701
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5666
DraI TTT'AAA
Cuts at: 3005 3005
Size: 5701
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4708 809
Size: 507 3392 1802
Fragments arranged by size:
3392 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2280 2548 2799
Size: 54 1425 50 275 18 450 268 251
Cuts at: 2799 2853 2904 3148 4037 5666 8
Size: 54 51 244 889 1629 43
Fragments arranged by size:
1629 1425 889 450 275 268 251 244
54 54 51 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2548 3148 1537
Size: 275 736 600 4090
Fragments arranged by size:
4090 736 600 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2223 3631 1353
Size: 870 1408 3423
Fragments arranged by size:
3423 1408 870
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2199 4288 4662 4808 387
Size: 1064 138 610 2089 374 146 1280
Fragments arranged by size:
2089 1280 1064 610 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2197 2321 2798 2962 3764 4361 5148
Size: 393 565 124 477 164 802 597 787
Cuts at: 5148 5416 1239
Size: 268 1524
Fragments arranged by size:
1524 802 787 597 565 477 393 268
164 124
EcoO109I rG'GnC_Cy
Cuts at: 2217 2218 2686 2217
Size: 1 468 5232
Fragments arranged by size:
5232 468 1
EcoRI G'AATT_C
Cuts at: 3010 3388 3010
Size: 378 5323
FspI TGC'GCA
Cuts at: 2585 4207 2585
Size: 1622 4079
HaeII r_GCGC'y
Cuts at: 1678 4848 1678
Size: 3170 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2879 2911 4342 4374 4416
Size: 32 135 32 1307 32 1431 32 42
Cuts at: 4416 4448 1373
Size: 32 2626
Fragments arranged by size:
2626 1431 1307 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3045 3291 669
Size: 988 281 1107 246 3079
Fragments arranged by size:
3079 1107 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5701
HpaI GTT'AAC
Cuts at: 3291 3291
Size: 5701
KpnI G_GTAC'C
Cuts at: 2680 3026 2680
Size: 346 5355
MfeI C'AATT_G
Cuts at: 3300 3300
Size: 5701
MluI A'CGCG_T
Cuts at: 5577 5577
Size: 5701
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4815 4999 1305
Size: 3510 184 2007
Fragments arranged by size:
3510 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2282 10
Size: 54 1425 343 450 3429
Fragments arranged by size:
3429 1425 450 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2921 3659 4018 4177 518
Size: 648 1755 738 359 159 2042
Fragments arranged by size:
2042 1755 738 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2148 2248 2599 2795 3117 3794 4942 5187
Size: 542 100 351 196 322 677 1148 245
Cuts at: 5187 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 542 351 322 245 196
100
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4210
Fragments arranged by size:
4210 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5701
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5701
NotI GC'GGCC_GC
Cuts at: 2548 3148 2548
Size: 600 5101
NspI r_CATG'y
Cuts at: 1132 1229 2845 3059 4604 1132
Size: 97 1616 214 1545 2229
Fragments arranged by size:
2229 1616 1545 214 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5701
PciI A'CATG_T
Cuts at: 4600 4600
Size: 5701
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5701
PmeI GTTT'AAAC
Cuts at: 3005 3005
Size: 5701
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3770 3802 5283 5315 3770
Size: 32 1481 32 4156
Fragments arranged by size:
4156 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2686 2686
Size: 5701
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5701
PsiI TTA'TAA
Cuts at: 3271 3271
Size: 5701
PspOMI G'GGCC_C
Cuts at: 2217 2217
Size: 5701
PspXI vC'TCGA_Gb
Cuts at: 2513 2513
Size: 5701
PstI C_TGCA'G
Cuts at: 830 2502 3053 830
Size: 1672 551 3478
Fragments arranged by size:
3478 1672 551
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5701
PvuII CAG'CTG
Cuts at: 2248 2248
Size: 5701
SacII CC_GC'GG
Cuts at: 2796 2796
Size: 5701
SalI G'TCGA_C
Cuts at: 1936 3043 1936
Size: 1107 4594
SbfI CC_TGCA'GG
Cuts at: 3053 3053
Size: 5701
ScaI AGT'ACT
Cuts at: 3949 5661 3949
Size: 1712 3989
SfcI C'TryA_G
Cuts at: 826 1045 2498 2681 3049 4184 4865 5056 826
Size: 219 1453 183 368 1135 681 191 1471
Fragments arranged by size:
1471 1453 1135 681 368 219 191 183
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5701
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5701
SmaI CCC'GGG
Cuts at: 1872 2362 3028 1872
Size: 490 666 4545
Fragments arranged by size:
4545 666 490
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2459 2513 3805 4706 4968 820
Size: 197 926 516 54 1292 901 262 1553
Fragments arranged by size:
1553 1292 926 901 516 262 197 54
SnaBI TAC'GTA
Cuts at: 493 3492 493
Size: 2999 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5701
SphI G_CATG'C
Cuts at: 1132 2845 3059 1132
Size: 1713 214 3774
Fragments arranged by size:
3774 1713 214
SspI AAT'ATT
Cuts at: 5 52 3625 5
Size: 47 3573 2081
Fragments arranged by size:
3573 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5701
StyI C'CwwG_G
Cuts at: 513 1065 2004 2478 3140 513
Size: 552 939 474 662 3074
Fragments arranged by size:
3074 939 662 552 474
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2848 3728 3745 3898 4083 4528 325
Size: 1255 1268 880 17 153 185 445 1498
Fragments arranged by size:
1498 1268 1255 880 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3947 5659 96
Size: 274 80 33 51 1443 1970 1712 138
Fragments arranged by size:
1970 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2063 2095 5661 5693 2063
Size: 32 3566 32 2071
Fragments arranged by size:
3566 2071 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5701
XbaI T'CTAG_A
Cuts at: 3037 3037
Size: 5701
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2610 2610
Size: 5701
XhoI C'TCGA_G
Cuts at: 1943 2513 1943
Size: 570 5131
XmaI C'CCGG_G
Cuts at: 1870 2360 3026 1870
Size: 490 666 4545
Fragments arranged by size:
4545 666 490
XmnI GAAnn'nnTTC
Cuts at: 3830 3830
Size: 5701
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3509 276
Size: 53 83 186 936 1975 2468
Fragments arranged by size:
2468 1975 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII SacII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PsrI RsrII SacI
SanDI SapI SexAI SfiI SfoI SrfI SwaI