Restriction map for
pFN21AB9697
(Circular) (Six-base) MAPSORT of: pFN21AB9697.seq Check: 4191 from: 1 to: 5515
pFN21AB9697 5515 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3325 278
Size: 53 83 186 936 1789 2468
Fragments arranged by size:
2468 1789 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2858 1937
Size: 921 4594
Acc65I G'GTAC_C
Cuts at: 2836 2836
Size: 5515
AclI AA'CG_TT
Cuts at: 1563 3642 4015 1563
Size: 2079 373 3063
Fragments arranged by size:
3063 2079 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3578 4962 5230 1632
Size: 1946 1384 268 1917
Fragments arranged by size:
1946 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5318
AflIII A'CryG_T
Cuts at: 1203 4414 5391 1203
Size: 3211 977 1327
Fragments arranged by size:
3211 1327 977
AgeI A'CCGG_T
Cuts at: 4346 4346
Size: 5515
AhdI GACnn_n'nnGTC
Cuts at: 4244 4244
Size: 5515
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5515
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5515
AlwNI CAG_nnn'CTG
Cuts at: 2530 2571 3258 4830 2530
Size: 41 687 1572 3215
Fragments arranged by size:
3215 1572 687 41
ApaLI G'TGCA_C
Cuts at: 2177 3572 4728 2177
Size: 1395 1156 2964
Fragments arranged by size:
2964 1395 1156
ApoI r'AATT_y
Cuts at: 1493 2824 3050 3202 5311 1493
Size: 1331 226 152 2109 1697
Fragments arranged by size:
2109 1697 1331 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4069 160
Size: 872 3037 1606
Fragments arranged by size:
3037 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5515
AvaI C'yCGr_G
Cuts at: 1870 1943 2468 2840 1870
Size: 73 525 372 4545
Fragments arranged by size:
4545 525 372 73
BamHI G'GATC_C
Cuts at: 2845 3212 2845
Size: 367 5148
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2065 2305 2594 2799 2836
Size: 325 217 627 278 240 289 205 37
Cuts at: 2836 4291 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 325 289 278 240 217
205 37
BanII G_rGCy'C
Cuts at: 1391 2223 2426 2469 2481 2834 1391
Size: 832 203 43 12 353 4072
Fragments arranged by size:
4072 832 353 203 43 12
BbeI G_GCGC'C
Cuts at: 2803 2803
Size: 5515
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2064 928
Size: 971 165 4379
Fragments arranged by size:
4379 971 165
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3706 3740 1903
Size: 34 1769 34 3678
Fragments arranged by size:
3678 1769 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3407 4617 3407
Size: 1210 4305
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5515
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2784 4126 136
Size: 107 122 71 631 605 1112 1342 1525
Fragments arranged by size:
1525 1342 1112 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5510 5510
Size: 5515
BlpI GC'TnA_GC
Cuts at: 2932 2932
Size: 5515
Bme1580I G_kGCm'C
Cuts at: 1790 2070 2181 2597 3576 4732 1790
Size: 280 111 416 979 1156 2573
Fragments arranged by size:
2573 1156 979 416 280 111
BmgBI CAC'GTC
Cuts at: 1318 2287 1318
Size: 969 4546
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2164 2313 3246
Size: 416 53 83 399 24 747 149 933
Cuts at: 3246 4204 442
Size: 958 1753
Fragments arranged by size:
1753 958 933 747 416 399 149 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5515
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2207 2294 2345 2453 2483 4175 1239
Size: 968 87 51 108 30 1692 2579
Fragments arranged by size:
2579 1692 968 108 87 51 30
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3640 4505 4803 3640
Size: 865 298 4352
Fragments arranged by size:
4352 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2341 4178 882
Size: 617 842 1837 2219
Fragments arranged by size:
2219 1837 842 617
BsaAI yAC'GTr
Cuts at: 493 1204 2176 3306 493
Size: 711 972 1130 2702
Fragments arranged by size:
2702 1130 972 711
BsaBI GATnn'nnATC
Cuts at: 5509 5509
Size: 5515
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2800 3322 3704 4339
Size: 53 83 186 936 1267 522 382 635
Cuts at: 4339 275
Size: 1451
Fragments arranged by size:
1451 1267 936 635 522 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3948 4346 4620 4767 5178 3948
Size: 398 274 147 411 4285
Fragments arranged by size:
4285 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2321 2351 1178
Size: 30 165 30 918 30 4342
Fragments arranged by size:
4342 918 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2417 2420 1186
Size: 1231 3 4281
Fragments arranged by size:
4281 1231 3
BseYI C'CCAG_C
Cuts at: 2380 2385 2390 2640 2773 4718 5214 2380
Size: 5 5 250 133 1945 496 2681
Fragments arranged by size:
2681 1945 496 250 133 5 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2252 1163
Size: 573 516 4426
Fragments arranged by size:
4426 573 516
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2965 3726 4754 1540
Size: 275 186 964 761 1028 2301
Fragments arranged by size:
2301 1028 964 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2181 2223 3576 3661 4732 2181
Size: 42 1353 85 1071 2964
Fragments arranged by size:
2964 1353 1071 85 42
BsmI GAATG_Cn'
Cuts at: 3026 3119 3026
Size: 93 5422
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2070 2181 2223 2426 2469 2481 2597
Size: 399 280 111 42 203 43 12 116
Cuts at: 2597 2834 3576 3661 4732 1391
Size: 237 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 280 237 203 116
111 85 43 42 12
BspEI T'CCGG_A
Cuts at: 5178 5178
Size: 5515
BspHI T'CATG_A
Cuts at: 3404 5134 3404
Size: 1730 3785
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2352 2547 2870 844
Size: 1508 195 323 3489
Fragments arranged by size:
3489 1508 323 195
BsrBI CCG'CTC
Cuts at: 1394 1823 2416 2797 3402 1394
Size: 429 593 381 605 3507
Fragments arranged by size:
3507 605 593 429 381
BsrDI GCAATG_nn'
Cuts at: 65 1248 4010 4184 65
Size: 1183 2762 174 1396
Fragments arranged by size:
2762 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4159 4346 1700
Size: 110 2349 187 2869
Fragments arranged by size:
2869 2349 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5515
BssHII G'CGCG_C
Cuts at: 1925 2690 2692 2694 1925
Size: 765 2 2 4746
Fragments arranged by size:
4746 765 2 2
BssSI C'ACGA_G
Cuts at: 2102 3575 4587 2102
Size: 1473 1012 3030
Fragments arranged by size:
3030 1473 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5515
BstBI TT'CG_AA
Cuts at: 4319 4319
Size: 5515
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5515
BstYI r'GATC_y
Cuts at: 1972 2080 2511 2845 3212 3597 3614 5066 5152
Size: 108 431 334 367 385 17 1452 86
Cuts at: 5152 5164 5510 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 431 385 367 346 334 108
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2301 2779 513
Size: 552 1236 478 3249
Fragments arranged by size:
3249 1236 552 478
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4776
Fragments arranged by size:
4776 683 56
BtsI GCAGTG_nn'
Cuts at: 2131 3031 3825 3845 2131
Size: 900 794 20 3801
Fragments arranged by size:
3801 900 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5515
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5480
DraI TTT'AAA
Cuts at: 2819 2819
Size: 5515
DraIII CAC_nnn'GTG
Cuts at: 2176 2176
Size: 5515
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4522 809
Size: 507 3206 1802
Fragments arranged by size:
3206 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2326 2790 2962
Size: 54 1425 50 275 18 496 464 172
Cuts at: 2962 3851 5480 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 496 464 275 172 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2962 1537
Size: 275 1150 4090
Fragments arranged by size:
4090 1150 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3445 1353
Size: 2092 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2468 2504 4102 4476 4622 387
Size: 1064 138 879 36 1598 374 146 1280
Fragments arranged by size:
1598 1280 1064 879 374 146 138 36
EcoICRI GAG'CTC
Cuts at: 2221 2221
Size: 5515
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2207 2294 2345 2453 2483 3578 4175
Size: 393 575 87 51 108 30 1095 597
Cuts at: 4175 4962 5230 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1095 787 597 575 393 268 108
87 51 30
EcoO109I rG'GnC_Cy
Cuts at: 2785 2785
Size: 5515
EcoRI G'AATT_C
Cuts at: 2824 3202 2824
Size: 378 5137
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2614 2614
Size: 5515
FspI TGC'GCA
Cuts at: 2702 4021 2702
Size: 1319 4196
HaeII r_GCGC'y
Cuts at: 1678 2630 2803 4662 1678
Size: 952 173 1859 2531
Fragments arranged by size:
2531 1859 952 173
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4156 4188 4230 4262 1373
Size: 32 135 32 2584 32 42 32 2626
Fragments arranged by size:
2626 2584 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2859 3105 669
Size: 988 281 921 246 3079
Fragments arranged by size:
3079 988 921 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5515
HpaI GTT'AAC
Cuts at: 3105 3105
Size: 5515
KasI G'GCGC_C
Cuts at: 2799 2799
Size: 5515
KpnI G_GTAC'C
Cuts at: 2840 2840
Size: 5515
MfeI C'AATT_G
Cuts at: 3114 3114
Size: 5515
MluI A'CGCG_T
Cuts at: 5391 5391
Size: 5515
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2514 4629 4813 1305
Size: 1209 2115 184 2007
Fragments arranged by size:
2115 2007 1209 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2328 10
Size: 54 1425 343 496 3197
Fragments arranged by size:
3197 1425 496 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3473 3832 3991 518
Size: 648 2307 359 159 2042
Fragments arranged by size:
2307 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2245 2550 2640 2763 2781 2931 3608 4756
Size: 639 305 90 123 18 150 677 1148
Cuts at: 4756 5001 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 639 305 245 150 123
90 18
NarI GG'CG_CC
Cuts at: 2800 2800
Size: 5515
NcoI C'CATG_G
Cuts at: 513 1065 2301 513
Size: 552 1236 3727
Fragments arranged by size:
3727 1236 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5515
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5515
NotI GC'GGCC_GC
Cuts at: 2962 2962
Size: 5515
NspI r_CATG'y
Cuts at: 1132 1229 2873 4418 1132
Size: 97 1644 1545 2229
Fragments arranged by size:
2229 1644 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5515
PciI A'CATG_T
Cuts at: 4414 4414
Size: 5515
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2686 1108
Size: 1578 3937
PmeI GTTT'AAAC
Cuts at: 2819 2819
Size: 5515
PmlI CAC'GTG
Cuts at: 2176 2176
Size: 5515
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3584 3616 5097 5129 3584
Size: 32 1481 32 3970
Fragments arranged by size:
3970 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5515
PsiI TTA'TAA
Cuts at: 3085 3085
Size: 5515
PstI C_TGCA'G
Cuts at: 830 2250 2867 830
Size: 1420 617 3478
Fragments arranged by size:
3478 1420 617
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5515
PvuII CAG'CTG
Cuts at: 2245 2245
Size: 5515
SacI G_AGCT'C
Cuts at: 2223 2223
Size: 5515
SacII CC_GC'GG
Cuts at: 2782 2782
Size: 5515
SalI G'TCGA_C
Cuts at: 1936 2857 1936
Size: 921 4594
SbfI CC_TGCA'GG
Cuts at: 2867 2867
Size: 5515
ScaI AGT'ACT
Cuts at: 3763 5475 3763
Size: 1712 3803
SexAI A'CCwGG_T
Cuts at: 2061 2331 2061
Size: 270 5245
SfcI C'TryA_G
Cuts at: 826 1045 2246 2863 3998 4679 4870 826
Size: 219 1201 617 1135 681 191 1471
Fragments arranged by size:
1471 1201 1135 681 617 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2784 2784
Size: 5515
SfoI GGC'GCC
Cuts at: 2801 2801
Size: 5515
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5515
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5515
SmaI CCC'GGG
Cuts at: 1872 2470 2842 1872
Size: 598 372 4545
Fragments arranged by size:
4545 598 372
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3619 4520 4782 820
Size: 197 926 1676 901 262 1553
Fragments arranged by size:
1676 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3306 493
Size: 2813 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5515
SphI G_CATG'C
Cuts at: 1132 2873 1132
Size: 1741 3774
SrfI GCCC'GGGC
Cuts at: 2470 2470
Size: 5515
SspI AAT'ATT
Cuts at: 5 52 3439 5
Size: 47 3387 2081
Fragments arranged by size:
3387 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5515
StyI C'CwwG_G
Cuts at: 513 1065 2301 2954 513
Size: 552 1236 653 3074
Fragments arranged by size:
3074 1236 653 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2566 3542 3559 3712 3897 4342 325
Size: 1255 986 976 17 153 185 445 1498
Fragments arranged by size:
1498 1255 986 976 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3761 5473 96
Size: 274 80 33 51 1443 1784 1712 138
Fragments arranged by size:
1784 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2321 2353 5475 5507 2321
Size: 32 3122 32 2329
Fragments arranged by size:
3122 2329 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5515
XbaI T'CTAG_A
Cuts at: 2851 2851
Size: 5515
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2321 2321
Size: 5515
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5515
XmaI C'CCGG_G
Cuts at: 1870 2468 2840 1870
Size: 598 372 4545
Fragments arranged by size:
4545 598 372
XmnI GAAnn'nnTTC
Cuts at: 2031 2210 3644 2031
Size: 179 1434 3902
Fragments arranged by size:
3902 1434 179
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3323 276
Size: 53 83 186 936 1789 2468
Fragments arranged by size:
2468 1789 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PstI PvuI PvuII SacI SacII SalI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI
PacI PfoI PpuMI PspOMI PspXI PsrI RsrII SanDI
SapI SwaI