Restriction map for
pFN21AB9694
(Circular) (Six-base) MAPSORT of: pFN21AB9694.seq Check: 520 from: 1 to: 5461
pFN21AB9694 5461 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3271 278
Size: 53 83 186 936 1735 2468
Fragments arranged by size:
2468 1735 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2193 2804 1937
Size: 256 611 4594
Fragments arranged by size:
4594 611 256
Acc65I G'GTAC_C
Cuts at: 2782 2782
Size: 5461
AclI AA'CG_TT
Cuts at: 1563 3588 3961 1563
Size: 2025 373 3063
Fragments arranged by size:
3063 2025 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3524 4908 5176 1632
Size: 1892 1384 268 1917
Fragments arranged by size:
1917 1892 1384 268
AfeI AGC'GCT
Cuts at: 2027 2027
Size: 5461
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5264
AflIII A'CryG_T
Cuts at: 1203 4360 5337 1203
Size: 3157 977 1327
Fragments arranged by size:
3157 1327 977
AgeI A'CCGG_T
Cuts at: 2700 4292 2700
Size: 1592 3869
AhdI GACnn_n'nnGTC
Cuts at: 4190 4190
Size: 5461
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5461
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5461
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2198 2230 2198
Size: 32 5429
AlwNI CAG_nnn'CTG
Cuts at: 2644 3204 4776 2644
Size: 560 1572 3329
Fragments arranged by size:
3329 1572 560
ApaLI G'TGCA_C
Cuts at: 2258 3518 4674 2258
Size: 1260 1156 3045
Fragments arranged by size:
3045 1260 1156
ApoI r'AATT_y
Cuts at: 1493 2770 2996 3148 5257 1493
Size: 1277 226 152 2109 1697
Fragments arranged by size:
2109 1697 1277 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4015 160
Size: 872 2983 1606
Fragments arranged by size:
2983 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5461
AvaI C'yCGr_G
Cuts at: 1870 1943 2426 2786 1870
Size: 73 483 360 4545
Fragments arranged by size:
4545 483 360 73
BamHI G'GATC_C
Cuts at: 2347 2472 2791 3158 2347
Size: 125 319 367 4650
Fragments arranged by size:
4650 367 319 125
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2017 2266 2546 2782 4237
Size: 325 217 627 230 249 280 236 1455
Cuts at: 4237 618
Size: 1842
Fragments arranged by size:
1842 1455 627 325 280 249 236 230
217
BanII G_rGCy'C
Cuts at: 1391 2780 1391
Size: 1389 4072
BbeI G_GCGC'C
Cuts at: 2021 2550 2021
Size: 529 4932
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4490
BbvCI CC'TCA_GC
Cuts at: 2576 2576
Size: 5461
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3652 3686 1903
Size: 34 1715 34 3678
Fragments arranged by size:
3678 1715 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3353 4563 3353
Size: 1210 4251
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5461
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4072 136
Size: 107 122 71 631 605 2400 1525
Fragments arranged by size:
2400 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5456 5456
Size: 5461
BlpI GC'TnA_GC
Cuts at: 2878 2878
Size: 5461
Bme1580I G_kGCm'C
Cuts at: 1790 2262 3522 4678 1790
Size: 472 1260 1156 2573
Fragments arranged by size:
2573 1260 1156 472
BmgBI CAC'GTC
Cuts at: 1318 2746 1318
Size: 1428 4033
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3192 4150 442
Size: 416 53 83 399 24 1775 958 1753
Fragments arranged by size:
1775 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5461
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2245 2245
Size: 5461
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2110 2666 4121 1239
Size: 871 556 1455 2579
Fragments arranged by size:
2579 1455 871 556
Bpu10I CC'TnA_GC
Cuts at: 2576 2576
Size: 5461
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3586 4451 4749 3586
Size: 865 298 4298
Fragments arranged by size:
4298 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2233 4124 882
Size: 617 734 1891 2219
Fragments arranged by size:
2219 1891 734 617
BsaAI yAC'GTr
Cuts at: 493 1204 2539 3252 493
Size: 711 1335 713 2702
Fragments arranged by size:
2702 1335 713 711
BsaBI GATnn'nnATC
Cuts at: 5455 5455
Size: 5461
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2018 2190 2547 3268
Size: 53 83 186 936 485 172 357 721
Cuts at: 3268 3650 4285 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 721 635 485 382 357 186
172 83 53
BsaWI w'CCGG_w
Cuts at: 2700 2731 3894 4292 4566 4713 5124 2700
Size: 31 1163 398 274 147 411 3037
Fragments arranged by size:
3037 1163 411 398 274 147 31
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2108 2138 1178
Size: 30 165 30 705 30 4501
Fragments arranged by size:
4501 705 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2296 1186
Size: 1110 4351
BseYI C'CCAG_C
Cuts at: 2496 4664 5160 2496
Size: 2168 496 2797
Fragments arranged by size:
2797 2168 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4888
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2166 2911 3672 4700 1540
Size: 275 186 165 745 761 1028 2301
Fragments arranged by size:
2301 1028 761 745 275 186 165
BsiHKAI G_wGCw'C
Cuts at: 2262 3522 3607 4678 2262
Size: 1260 85 1071 3045
Fragments arranged by size:
3045 1260 1071 85
BsmI GAATG_Cn'
Cuts at: 2972 3065 2972
Size: 93 5368
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2262 2780 3522 3607 4678 1391
Size: 399 472 518 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 518 472 399 85
BspEI T'CCGG_A
Cuts at: 2731 5124 2731
Size: 2393 3068
BspHI T'CATG_A
Cuts at: 3350 5080 3350
Size: 1730 3731
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2816 844
Size: 1972 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3348 1394
Size: 429 1525 3507
Fragments arranged by size:
3507 1525 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3956 4130 65
Size: 1183 2708 174 1396
Fragments arranged by size:
2708 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2014 2180 2700 4105 4292 1700
Size: 110 204 166 520 1405 187 2869
Fragments arranged by size:
2869 1405 520 204 187 166 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5461
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5461
BssSI C'ACGA_G
Cuts at: 3521 4533 3521
Size: 1012 4449
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2266 794
Size: 1472 3989
BstBI TT'CG_AA
Cuts at: 4265 4265
Size: 5461
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5461
BstYI r'GATC_y
Cuts at: 1972 2347 2472 2791 3158 3543 3560 5012 5098
Size: 375 125 319 367 385 17 1452 86
Cuts at: 5098 5110 5456 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 385 375 367 346 319 125
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2103 513
Size: 552 1038 3871
Fragments arranged by size:
3871 1038 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2384 487
Size: 683 56 1158 3564
Fragments arranged by size:
3564 1158 683 56
BtsI GCAGTG_nn'
Cuts at: 2977 3771 3791 2977
Size: 794 20 4647
Fragments arranged by size:
4647 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5461
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5426
DraI TTT'AAA
Cuts at: 2765 2765
Size: 5461
DraIII CAC_nnn'GTG
Cuts at: 2266 2539 2266
Size: 273 5188
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4468 809
Size: 507 3152 1802
Fragments arranged by size:
3152 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2052 2163 2286
Size: 54 1425 50 275 18 222 111 123
Cuts at: 2286 2908 3797 5426 8
Size: 622 889 1629 43
Fragments arranged by size:
1629 1425 889 622 275 222 123 111
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2163 2908 1537
Size: 275 351 745 4090
Fragments arranged by size:
4090 745 351 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3391 1353
Size: 2038 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4048 4422 4568 387
Size: 1064 138 2459 374 146 1280
Fragments arranged by size:
2459 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2110 2666 3524 4121 4908 5176 1239
Size: 393 478 556 858 597 787 268 1524
Fragments arranged by size:
1524 858 787 597 556 478 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2089 2089
Size: 5461
EcoRI G'AATT_C
Cuts at: 2770 3148 2770
Size: 378 5083
EcoRV GAT'ATC
Cuts at: 2390 2390
Size: 5461
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2047 2047
Size: 5461
FspI TGC'GCA
Cuts at: 3967 3967
Size: 5461
HaeII r_GCGC'y
Cuts at: 1678 2021 2029 2550 4608 1678
Size: 343 8 521 2058 2531
Fragments arranged by size:
2531 2058 521 343 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2735 2767 4102 4134 4176
Size: 32 135 32 1163 32 1335 32 42
Cuts at: 4176 4208 1373
Size: 32 2626
Fragments arranged by size:
2626 1335 1163 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2805 3051 669
Size: 988 281 867 246 3079
Fragments arranged by size:
3079 988 867 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5461
HpaI GTT'AAC
Cuts at: 3051 3051
Size: 5461
KasI G'GCGC_C
Cuts at: 2017 2546 2017
Size: 529 4932
KpnI G_GTAC'C
Cuts at: 2786 2786
Size: 5461
MfeI C'AATT_G
Cuts at: 3060 3060
Size: 5461
MluI A'CGCG_T
Cuts at: 5337 5337
Size: 5461
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2223 4575 4759 1305
Size: 918 2352 184 2007
Fragments arranged by size:
2352 2007 918 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2054 10
Size: 54 1425 343 222 3417
Fragments arranged by size:
3417 1425 343 222 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3419 3778 3937 518
Size: 648 2253 359 159 2042
Fragments arranged by size:
2253 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2105 2344 2563 2877 3554 4702 4947 1606
Size: 499 239 219 314 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 499 314 245 239 219
NaeI GCC'GGC
Cuts at: 2016 2016
Size: 5461
NarI GG'CG_CC
Cuts at: 2018 2547 2018
Size: 529 4932
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4909
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5461
NgoMIV G'CCGG_C
Cuts at: 2014 2014
Size: 5461
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5461
NotI GC'GGCC_GC
Cuts at: 2908 2908
Size: 5461
NspI r_CATG'y
Cuts at: 1132 1229 2819 4364 1132
Size: 97 1590 1545 2229
Fragments arranged by size:
2229 1590 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5461
PciI A'CATG_T
Cuts at: 4360 4360
Size: 5461
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5461
PmeI GTTT'AAAC
Cuts at: 2765 2765
Size: 5461
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3530 3562 5043 5075 3530
Size: 32 1481 32 3916
Fragments arranged by size:
3916 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2089 2089
Size: 5461
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5461
PsiI TTA'TAA
Cuts at: 3031 3031
Size: 5461
PstI C_TGCA'G
Cuts at: 830 2813 830
Size: 1983 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5461
PvuII CAG'CTG
Cuts at: 2563 2563
Size: 5461
SacII CC_GC'GG
Cuts at: 2106 2106
Size: 5461
SalI G'TCGA_C
Cuts at: 1936 2803 1936
Size: 867 4594
SbfI CC_TGCA'GG
Cuts at: 2813 2813
Size: 5461
ScaI AGT'ACT
Cuts at: 3709 5421 3709
Size: 1712 3749
SfcI C'TryA_G
Cuts at: 826 1045 2809 3944 4625 4816 826
Size: 219 1764 1135 681 191 1471
Fragments arranged by size:
1764 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2019 2548 2019
Size: 529 4932
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5461
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5461
SmaI CCC'GGG
Cuts at: 1872 2788 1872
Size: 916 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3565 4466 4728 820
Size: 197 926 1622 901 262 1553
Fragments arranged by size:
1622 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3252 493
Size: 2759 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5461
SphI G_CATG'C
Cuts at: 1132 2819 1132
Size: 1687 3774
SspI AAT'ATT
Cuts at: 5 52 3385 5
Size: 47 3333 2081
Fragments arranged by size:
3333 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5461
StyI C'CwwG_G
Cuts at: 513 1065 2055 2407 2900 513
Size: 552 990 352 493 3074
Fragments arranged by size:
3074 990 552 493 352
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3488 3505 3658 3843 4288 325
Size: 1255 1908 17 153 185 445 1498
Fragments arranged by size:
1908 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3707 5419 96
Size: 274 80 33 51 1443 1730 1712 138
Fragments arranged by size:
1730 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5421 5453 5421
Size: 32 5429
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5461
XbaI T'CTAG_A
Cuts at: 2797 2797
Size: 5461
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2044 2206 2044
Size: 162 5299
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5461
XmaI C'CCGG_G
Cuts at: 1870 2786 1870
Size: 916 4545
XmnI GAAnn'nnTTC
Cuts at: 3590 3590
Size: 5461
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3269 276
Size: 53 83 186 936 1735 2468
Fragments arranged by size:
2468 1735 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SacII SalI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BaeI BfrBI BsiWI BsmBI
BstXI BstZ17I Bsu36I EcoICRI EcoNI FseI FspAI NruI
NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII
SacI SanDI SapI SexAI SfiI SrfI SwaI