Restriction map for
pFN21AB9693
(Circular) (Six-base) MAPSORT of: pFN21AB9693.seq Check: 2530 from: 1 to: 5461
pFN21AB9693 5461 bp
With 182 enzymes: SgfI *
December 28, 2011 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2244 2454 2244
Size: 210 5251
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3271 278
Size: 53 83 186 936 1735 2468
Fragments arranged by size:
2468 1735 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2804 1937
Size: 867 4594
Acc65I G'GTAC_C
Cuts at: 2782 2782
Size: 5461
AclI AA'CG_TT
Cuts at: 1563 3588 3961 1563
Size: 2025 373 3063
Fragments arranged by size:
3063 2025 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3524 4908 5176 1632
Size: 1892 1384 268 1917
Fragments arranged by size:
1917 1892 1384 268
AfeI AGC'GCT
Cuts at: 2561 2588 2561
Size: 27 5434
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5264
AflIII A'CryG_T
Cuts at: 1203 4360 5337 1203
Size: 3157 977 1327
Fragments arranged by size:
3157 1327 977
AgeI A'CCGG_T
Cuts at: 4292 4292
Size: 5461
AhdI GACnn_n'nnGTC
Cuts at: 4190 4190
Size: 5461
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5461
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5461
AlwNI CAG_nnn'CTG
Cuts at: 3204 4776 3204
Size: 1572 3889
ApaI G_GGCC'C
Cuts at: 2114 2213 2114
Size: 99 5362
ApaLI G'TGCA_C
Cuts at: 2078 3518 4674 2078
Size: 1440 1156 2865
Fragments arranged by size:
2865 1440 1156
ApoI r'AATT_y
Cuts at: 1493 2770 2996 3148 5257 1493
Size: 1277 226 152 2109 1697
Fragments arranged by size:
2109 1697 1277 226 152
AscI GG'CGCG_CC
Cuts at: 2141 2141
Size: 5461
AseI AT'TA_AT
Cuts at: 160 1032 4015 160
Size: 872 2983 1606
Fragments arranged by size:
2983 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5461
AvaI C'yCGr_G
Cuts at: 1870 1943 2099 2162 2170 2206 2287 2338 2786
Size: 73 156 63 8 36 81 51 448
Cuts at: 2786 1870
Size: 4545
Fragments arranged by size:
4545 448 156 81 73 63 51 36
8
BamHI G'GATC_C
Cuts at: 2791 3158 2791
Size: 367 5094
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2069 2216 2227 2294 2354
Size: 325 217 627 282 147 11 67 60
Cuts at: 2354 2658 2666 2782 4237 618
Size: 304 8 116 1455 1842
Fragments arranged by size:
1842 1455 627 325 304 282 217 147
116 67 60 11 8
BanII G_rGCy'C
Cuts at: 1391 2114 2213 2720 2780 1391
Size: 723 99 507 60 4072
Fragments arranged by size:
4072 723 507 99 60
BbeI G_GCGC'C
Cuts at: 2073 2220 2231 2298 2358 2662 2073
Size: 147 11 67 60 304 4872
Fragments arranged by size:
4872 304 147 67 60 11
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4490
BbvCI CC'TCA_GC
Cuts at: 2317 2317
Size: 5461
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3652 3686 1903
Size: 34 1715 34 3678
Fragments arranged by size:
3678 1715 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3353 4563 3353
Size: 1210 4251
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5461
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2103 2225
Size: 107 122 71 631 605 337 94 122
Cuts at: 2225 2634 4072 136
Size: 409 1438 1525
Fragments arranged by size:
1525 1438 631 605 409 337 122 122
107 94 71
BglII A'GATC_T
Cuts at: 5456 5456
Size: 5461
BlpI GC'TnA_GC
Cuts at: 2878 2878
Size: 5461
Bme1580I G_kGCm'C
Cuts at: 1790 2082 2114 2213 2671 3522 4678 1790
Size: 292 32 99 458 851 1156 2573
Fragments arranged by size:
2573 1156 851 458 292 99 32
BmgBI CAC'GTC
Cuts at: 1318 2047 1318
Size: 729 4732
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3192 4150 442
Size: 416 53 83 399 24 1775 958 1753
Fragments arranged by size:
1775 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5461
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2173 2173
Size: 5461
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4121 1239
Size: 2882 2579
Bpu10I CC'TnA_GC
Cuts at: 2317 2317
Size: 5461
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3586 4451 4749 3586
Size: 865 298 4298
Fragments arranged by size:
4298 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4124 882
Size: 617 2625 2219
Fragments arranged by size:
2625 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3252 493
Size: 711 2048 2702
Fragments arranged by size:
2702 2048 711
BsaBI GATnn'nnATC
Cuts at: 5455 5455
Size: 5461
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2070 2217 2228 2295
Size: 53 83 186 936 537 147 11 67
Cuts at: 2295 2355 2659 3268 3650 4285 275
Size: 60 304 609 382 635 1451
Fragments arranged by size:
1451 936 635 609 537 382 304 186
147 83 67 60 53 11
BsaWI w'CCGG_w
Cuts at: 2538 3894 4292 4566 4713 5124 2538
Size: 1356 398 274 147 411 2875
Fragments arranged by size:
2875 1356 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5236
Fragments arranged by size:
5236 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2299 2302 2305 1186
Size: 1113 3 3 4342
Fragments arranged by size:
4342 1113 3 3
BseYI C'CCAG_C
Cuts at: 2684 2726 2738 2743 4664 5160 2684
Size: 42 12 5 1921 496 2985
Fragments arranged by size:
2985 1921 496 42 12 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4888
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2016 2911 3672 4700 1540
Size: 275 186 15 895 761 1028 2301
Fragments arranged by size:
2301 1028 895 761 275 186 15
BsiHKAI G_wGCw'C
Cuts at: 2052 2082 2582 3522 3607 4678 2052
Size: 30 500 940 85 1071 2835
Fragments arranged by size:
2835 1071 940 500 85 30
BsmI GAATG_Cn'
Cuts at: 2972 3065 2972
Size: 93 5368
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2052 2082 2114 2213 2582 2671 2720
Size: 399 262 30 32 99 369 89 49
Cuts at: 2720 2780 3522 3607 4678 1391
Size: 60 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 369 262 99 89
85 60 49 32 30
BspEI T'CCGG_A
Cuts at: 5124 5124
Size: 5461
BspHI T'CATG_A
Cuts at: 3350 5080 3350
Size: 1730 3731
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2244 2271 2454 2658 2816 844
Size: 1400 27 183 204 158 3489
Fragments arranged by size:
3489 1400 204 183 158 27
BsrBI CCG'CTC
Cuts at: 1394 1823 2514 3348 1394
Size: 429 691 834 3507
Fragments arranged by size:
3507 834 691 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3956 4130 65
Size: 1183 2708 174 1396
Fragments arranged by size:
2708 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2120 4105 4292 1700
Size: 110 310 1985 187 2869
Fragments arranged by size:
2869 1985 310 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5461
BssHII G'CGCG_C
Cuts at: 1925 2134 2141 1925
Size: 209 7 5245
Fragments arranged by size:
5245 209 7
BssSI C'ACGA_G
Cuts at: 3521 4533 3521
Size: 1012 4449
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5461
BstBI TT'CG_AA
Cuts at: 4265 4265
Size: 5461
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5461
BstXI CCAn_nnnn'nTGG
Cuts at: 2254 2254
Size: 5461
BstYI r'GATC_y
Cuts at: 1972 2791 3158 3543 3560 5012 5098 5110 5456
Size: 819 367 385 17 1452 86 12 346
Cuts at: 5456 1972
Size: 1977
Fragments arranged by size:
1977 1452 819 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2016 2073 2083 2223 513
Size: 552 951 57 10 140 3751
Fragments arranged by size:
3751 951 552 140 57 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4722
Fragments arranged by size:
4722 683 56
BtsI GCAGTG_nn'
Cuts at: 2605 2977 3771 3791 2605
Size: 372 794 20 4275
Fragments arranged by size:
4275 794 372 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5461
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5426
DraI TTT'AAA
Cuts at: 2765 2765
Size: 5461
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4468 809
Size: 507 3152 1802
Fragments arranged by size:
3152 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2013 2055 2241
Size: 54 1425 50 275 18 183 42 186
Cuts at: 2241 2256 2610 2709 2908 3797 5426 8
Size: 15 354 99 199 889 1629 43
Fragments arranged by size:
1629 1425 889 354 275 199 186 183
99 54 50 43 42 18 15
EagI C'GGCC_G
Cuts at: 1537 1812 2013 2908 1537
Size: 275 201 895 4090
Fragments arranged by size:
4090 895 275 201
EarI CTCTTCn'nnn_
Cuts at: 1353 2068 3391 1353
Size: 715 1323 3423
Fragments arranged by size:
3423 1323 715
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2286 2356 4048 4422 4568 387
Size: 1064 138 697 70 1692 374 146 1280
Fragments arranged by size:
1692 1280 1064 697 374 146 138 70
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3524 4121 4908 5176 1239
Size: 393 1892 597 787 268 1524
Fragments arranged by size:
1892 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2111 2166 2210 2111
Size: 55 44 5362
Fragments arranged by size:
5362 55 44
EcoRI G'AATT_C
Cuts at: 2770 3148 2770
Size: 378 5083
FspI TGC'GCA
Cuts at: 3967 3967
Size: 5461
HaeII r_GCGC'y
Cuts at: 1678 2073 2220 2231 2298 2358 2563 2590 2662
Size: 395 147 11 67 60 205 27 72
Cuts at: 2662 4608 1678
Size: 1946 2531
Fragments arranged by size:
2531 1946 395 205 147 72 67 60
27 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4102 4134 4176 4208 1373
Size: 32 135 32 2530 32 42 32 2626
Fragments arranged by size:
2626 2530 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2805 3051 669
Size: 988 281 867 246 3079
Fragments arranged by size:
3079 988 867 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5461
HpaI GTT'AAC
Cuts at: 3051 3051
Size: 5461
KasI G'GCGC_C
Cuts at: 2069 2216 2227 2294 2354 2658 2069
Size: 147 11 67 60 304 4872
Fragments arranged by size:
4872 304 147 67 60 11
KpnI G_GTAC'C
Cuts at: 2786 2786
Size: 5461
MfeI C'AATT_G
Cuts at: 3060 3060
Size: 5461
MluI A'CGCG_T
Cuts at: 5337 5337
Size: 5461
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2541 4575 4759 1305
Size: 1236 2034 184 2007
Fragments arranged by size:
2034 2007 1236 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2057 2612 10
Size: 54 1425 343 225 555 2859
Fragments arranged by size:
2859 1425 555 343 225 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3419 3778 3937 518
Size: 648 2253 359 159 2042
Fragments arranged by size:
2253 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2085 2225 2388 2877 3554 4702 4947 1606
Size: 479 140 163 489 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 489 479 245 163 140
NaeI GCC'GGC
Cuts at: 2122 2122
Size: 5461
NarI GG'CG_CC
Cuts at: 2070 2217 2228 2295 2355 2659 2070
Size: 147 11 67 60 304 4872
Fragments arranged by size:
4872 304 147 67 60 11
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4909
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5461
NgoMIV G'CCGG_C
Cuts at: 2120 2120
Size: 5461
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5461
NotI GC'GGCC_GC
Cuts at: 2908 2908
Size: 5461
NspI r_CATG'y
Cuts at: 1132 1229 2819 4364 1132
Size: 97 1590 1545 2229
Fragments arranged by size:
2229 1590 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5461
PciI A'CATG_T
Cuts at: 4360 4360
Size: 5461
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2239 1108
Size: 1131 4330
PfoI T'CCnGG_A
Cuts at: 2397 2397
Size: 5461
PmeI GTTT'AAAC
Cuts at: 2765 2765
Size: 5461
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3530 3562 5043 5075 3530
Size: 32 1481 32 3916
Fragments arranged by size:
3916 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2166 2166
Size: 5461
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5461
PsiI TTA'TAA
Cuts at: 3031 3031
Size: 5461
PspOMI G'GGCC_C
Cuts at: 2110 2209 2110
Size: 99 5362
PstI C_TGCA'G
Cuts at: 830 2705 2813 830
Size: 1875 108 3478
Fragments arranged by size:
3478 1875 108
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5461
SacII CC_GC'GG
Cuts at: 2086 2226 2086
Size: 140 5321
SalI G'TCGA_C
Cuts at: 1936 2803 1936
Size: 867 4594
SanDI GG'GwC_CC
Cuts at: 2166 2166
Size: 5461
SbfI CC_TGCA'GG
Cuts at: 2813 2813
Size: 5461
ScaI AGT'ACT
Cuts at: 3709 5421 3709
Size: 1712 3749
SfcI C'TryA_G
Cuts at: 826 1045 2701 2809 3944 4625 4816 826
Size: 219 1656 108 1135 681 191 1471
Fragments arranged by size:
1656 1471 1135 681 219 191 108
SfoI GGC'GCC
Cuts at: 2071 2218 2229 2296 2356 2660 2071
Size: 147 11 67 60 304 4872
Fragments arranged by size:
4872 304 147 67 60 11
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5461
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5461
SmaI CCC'GGG
Cuts at: 1872 2164 2172 2208 2289 2788 1872
Size: 292 8 36 81 499 4545
Fragments arranged by size:
4545 499 292 81 36 8
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3565 4466 4728 820
Size: 197 926 1622 901 262 1553
Fragments arranged by size:
1622 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3252 493
Size: 2759 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5461
SphI G_CATG'C
Cuts at: 1132 2819 1132
Size: 1687 3774
SrfI GCCC'GGGC
Cuts at: 2208 2208
Size: 5461
SspI AAT'ATT
Cuts at: 5 52 3385 5
Size: 47 3333 2081
Fragments arranged by size:
3333 2081 47
StuI AGG'CCT
Cuts at: 1517 2757 1517
Size: 1240 4221
StyI C'CwwG_G
Cuts at: 513 1065 2900 513
Size: 552 1835 3074
Fragments arranged by size:
3074 1835 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3488 3505 3658 3843 4288 325
Size: 1255 1908 17 153 185 445 1498
Fragments arranged by size:
1908 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3707 5419 96
Size: 274 80 33 51 1443 1730 1712 138
Fragments arranged by size:
1730 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5421 5453 5421
Size: 32 5429
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5461
XbaI T'CTAG_A
Cuts at: 2797 2797
Size: 5461
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5461
XmaI C'CCGG_G
Cuts at: 1870 2162 2170 2206 2287 2786 1870
Size: 292 8 36 81 499 4545
Fragments arranged by size:
4545 499 292 81 36 8
XmnI GAAnn'nnTTC
Cuts at: 2561 3590 2561
Size: 1029 4432
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3269 276
Size: 53 83 186 936 1735 2468
Fragments arranged by size:
2468 1735 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI SacII SalI SanDI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NruI
NsiI PacI PmlI PspXI PsrI PvuII RsrII SacI
SapI SexAI SfiI SwaI XcmI