Restriction map for
pFN21AB9692
(Circular) (Six-base) MAPSORT of: pFN21AB9692.seq Check: 8538 from: 1 to: 5434
pFN21AB9692 5434 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3244 278
Size: 53 83 186 936 1708 2468
Fragments arranged by size:
2468 1708 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2777 1937
Size: 840 4594
Acc65I G'GTAC_C
Cuts at: 2755 2755
Size: 5434
AclI AA'CG_TT
Cuts at: 1563 3561 3934 1563
Size: 1998 373 3063
Fragments arranged by size:
3063 1998 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3497 4881 5149 1632
Size: 1865 1384 268 1917
Fragments arranged by size:
1917 1865 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5237
AflIII A'CryG_T
Cuts at: 1203 4333 5310 1203
Size: 3130 977 1327
Fragments arranged by size:
3130 1327 977
AgeI A'CCGG_T
Cuts at: 4265 4265
Size: 5434
AhdI GACnn_n'nnGTC
Cuts at: 4163 4163
Size: 5434
AleI CACnn'nnGTG
Cuts at: 1166 2117 1166
Size: 951 4483
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5434
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2480 2512 2480
Size: 32 5402
AlwNI CAG_nnn'CTG
Cuts at: 2098 2479 2677 3177 4749 2098
Size: 381 198 500 1572 2783
Fragments arranged by size:
2783 1572 500 381 198
ApaI G_GGCC'C
Cuts at: 2689 2689
Size: 5434
ApaLI G'TGCA_C
Cuts at: 3491 4647 3491
Size: 1156 4278
ApoI r'AATT_y
Cuts at: 1493 2743 2969 3121 5230 1493
Size: 1250 226 152 2109 1697
Fragments arranged by size:
2109 1697 1250 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3988 160
Size: 872 2956 1606
Fragments arranged by size:
2956 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5434
AvaI C'yCGr_G
Cuts at: 1870 1943 2759 1870
Size: 73 816 4545
Fragments arranged by size:
4545 816 73
BamHI G'GATC_C
Cuts at: 2305 2764 3131 2305
Size: 459 367 4608
Fragments arranged by size:
4608 459 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2755 4210 618
Size: 325 217 627 968 1455 1842
Fragments arranged by size:
1842 1455 968 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2012 2378 2390 2689 2753 1391
Size: 621 366 12 299 64 4072
Fragments arranged by size:
4072 621 366 299 64 12
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4463
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3625 3659 1903
Size: 34 1688 34 3678
Fragments arranged by size:
3678 1688 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3326 4536 3326
Size: 1210 4224
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5434
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2693 4045 136
Size: 107 122 71 631 605 1021 1352 1525
Fragments arranged by size:
1525 1352 1021 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5429 5429
Size: 5434
BlpI GC'TnA_GC
Cuts at: 2851 2851
Size: 5434
Bme1580I G_kGCm'C
Cuts at: 1790 2689 3495 4651 1790
Size: 899 806 1156 2573
Fragments arranged by size:
2573 1156 899 806
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5434
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3165 4123 442
Size: 416 53 83 399 24 1748 958 1753
Fragments arranged by size:
1753 1748 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5434
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2501 2507 2684 2699 4094 1239
Size: 1262 6 177 15 1395 2579
Fragments arranged by size:
2579 1395 1262 177 15 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3559 4424 4722 3559
Size: 865 298 4271
Fragments arranged by size:
4271 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4097 882
Size: 617 2598 2219
Fragments arranged by size:
2598 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3225 493
Size: 711 2021 2702
Fragments arranged by size:
2702 2021 711
BsaBI GATnn'nnATC
Cuts at: 5428 5428
Size: 5434
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3241 3623 4258 275
Size: 53 83 186 936 1708 382 635 1451
Fragments arranged by size:
1708 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2592 3867 4265 4539 4686 5097 2592
Size: 1275 398 274 147 411 2929
Fragments arranged by size:
2929 1275 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2480 2510 1178
Size: 30 165 30 1077 30 4102
Fragments arranged by size:
4102 1077 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2378 2672 1186
Size: 1192 294 3948
Fragments arranged by size:
3948 1192 294
BseYI C'CCAG_C
Cuts at: 2129 2636 4637 5133 2129
Size: 507 2001 496 2430
Fragments arranged by size:
2430 2001 507 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2339 1163
Size: 573 603 4258
Fragments arranged by size:
4258 603 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2107 2884 3645 4673 1540
Size: 275 186 106 777 761 1028 2301
Fragments arranged by size:
2301 1028 777 761 275 186 106
BsiHKAI G_wGCw'C
Cuts at: 2012 3495 3580 4651 2012
Size: 1483 85 1071 2795
Fragments arranged by size:
2795 1483 1071 85
BsmI GAATG_Cn'
Cuts at: 2945 3038 2945
Size: 93 5341
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2378 2390 2689 2753 3495 3580
Size: 399 222 366 12 299 64 742 85
Cuts at: 3580 4651 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 366 299 222 85
64 12
BspEI T'CCGG_A
Cuts at: 5097 5097
Size: 5434
BspHI T'CATG_A
Cuts at: 3323 5053 3323
Size: 1730 3704
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2789 844
Size: 1945 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2683 3321 1394
Size: 429 860 638 3507
Fragments arranged by size:
3507 860 638 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3929 4103 65
Size: 1183 2681 174 1396
Fragments arranged by size:
2681 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4078 4265 1700
Size: 110 2268 187 2869
Fragments arranged by size:
2869 2268 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5434
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5434
BssSI C'ACGA_G
Cuts at: 3494 4506 3494
Size: 1012 4422
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2646 794
Size: 1852 3582
BstBI TT'CG_AA
Cuts at: 4238 4238
Size: 5434
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5434
BstXI CCAn_nnnn'nTGG
Cuts at: 2356 2356
Size: 5434
BstYI r'GATC_y
Cuts at: 1972 2305 2764 3131 3516 3533 4985 5071 5083
Size: 333 459 367 385 17 1452 86 12
Cuts at: 5083 5429 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 459 385 367 346 333 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2115 2179 2199 2229 2239 513
Size: 552 1050 64 20 30 10 3708
Fragments arranged by size:
3708 1050 552 64 30 20 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4695
Fragments arranged by size:
4695 683 56
BtsI GCAGTG_nn'
Cuts at: 2950 3744 3764 2950
Size: 794 20 4620
Fragments arranged by size:
4620 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5434
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5399
DraI TTT'AAA
Cuts at: 2738 2738
Size: 5434
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4441 809
Size: 507 3125 1802
Fragments arranged by size:
3125 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2104 2881 3770
Size: 54 1425 50 275 18 274 777 889
Cuts at: 3770 5399 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 777 275 274 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2104 2881 1537
Size: 275 292 777 4090
Fragments arranged by size:
4090 777 292 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2411 3364 1353
Size: 1058 953 3423
Fragments arranged by size:
3423 1058 953
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2714 4021 4395 4541 387
Size: 1064 138 1125 1307 374 146 1280
Fragments arranged by size:
1307 1280 1125 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2010 2010
Size: 5434
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2501 2507 2684 2699 3497 4094 4881
Size: 393 869 6 177 15 798 597 787
Cuts at: 4881 5149 1239
Size: 268 1524
Fragments arranged by size:
1524 869 798 787 597 393 268 177
15 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2360 2360
Size: 5434
EcoO109I rG'GnC_Cy
Cuts at: 2095 2245 2686 2095
Size: 150 441 4843
Fragments arranged by size:
4843 441 150
EcoRI G'AATT_C
Cuts at: 2743 3121 2743
Size: 378 5056
FspI TGC'GCA
Cuts at: 2301 3940 2301
Size: 1639 3795
HaeII r_GCGC'y
Cuts at: 1678 4581 1678
Size: 2903 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4075 4107 4149 4181 1373
Size: 32 135 32 2503 32 42 32 2626
Fragments arranged by size:
2626 2503 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2023 2778 3024 669
Size: 988 281 85 755 246 3079
Fragments arranged by size:
3079 988 755 281 246 85
HindIII A'AGCT_T
Cuts at: 748 2336 748
Size: 1588 3846
HpaI GTT'AAC
Cuts at: 3024 3024
Size: 5434
KpnI G_GTAC'C
Cuts at: 2759 2759
Size: 5434
MfeI C'AATT_G
Cuts at: 3033 3033
Size: 5434
MluI A'CGCG_T
Cuts at: 5310 5310
Size: 5434
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4548 4732 1305
Size: 3243 184 2007
Fragments arranged by size:
3243 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3612
Fragments arranged by size:
3612 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2117 3392 3751 3910 518
Size: 648 951 1275 359 159 2042
Fragments arranged by size:
2042 1275 951 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2136 2275 2352 2850 3527 4675 4920 1606
Size: 530 139 77 498 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 530 498 245 139 77
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4882
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5434
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5434
NotI GC'GGCC_GC
Cuts at: 2881 2881
Size: 5434
NspI r_CATG'y
Cuts at: 1132 1229 2792 4337 1132
Size: 97 1563 1545 2229
Fragments arranged by size:
2229 1563 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2551 1348
Size: 1203 4231
PciI A'CATG_T
Cuts at: 4333 4333
Size: 5434
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2098 2479 1108
Size: 990 381 4063
Fragments arranged by size:
4063 990 381
PfoI T'CCnGG_A
Cuts at: 2166 2166
Size: 5434
PmeI GTTT'AAAC
Cuts at: 2738 2738
Size: 5434
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2480 2512 3503 3535 5016 5048 2480
Size: 32 991 32 1481 32 2866
Fragments arranged by size:
2866 1481 991 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2095 2245 2095
Size: 150 5284
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5434
PsiI TTA'TAA
Cuts at: 3004 3004
Size: 5434
PspOMI G'GGCC_C
Cuts at: 2685 2685
Size: 5434
PstI C_TGCA'G
Cuts at: 830 2786 830
Size: 1956 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5434
SacI G_AGCT'C
Cuts at: 2012 2012
Size: 5434
SalI G'TCGA_C
Cuts at: 1936 2776 1936
Size: 840 4594
SanDI GG'GwC_CC
Cuts at: 2245 2245
Size: 5434
SapI GCTCTTCn'nnn_
Cuts at: 2411 2411
Size: 5434
SbfI CC_TGCA'GG
Cuts at: 2786 2786
Size: 5434
ScaI AGT'ACT
Cuts at: 3682 5394 3682
Size: 1712 3722
SexAI A'CCwGG_T
Cuts at: 2313 2313
Size: 5434
SfcI C'TryA_G
Cuts at: 826 1045 2461 2782 3917 4598 4789 826
Size: 219 1416 321 1135 681 191 1471
Fragments arranged by size:
1471 1416 1135 681 321 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5434
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5434
SmaI CCC'GGG
Cuts at: 1872 2761 1872
Size: 889 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3538 4439 4701 820
Size: 197 926 1595 901 262 1553
Fragments arranged by size:
1595 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3225 493
Size: 2732 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5434
SphI G_CATG'C
Cuts at: 1132 2792 1132
Size: 1660 3774
SspI AAT'ATT
Cuts at: 5 52 3358 5
Size: 47 3306 2081
Fragments arranged by size:
3306 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5434
StyI C'CwwG_G
Cuts at: 513 1065 2873 513
Size: 552 1808 3074
Fragments arranged by size:
3074 1808 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3461 3478 3631 3816 4261 325
Size: 1255 1881 17 153 185 445 1498
Fragments arranged by size:
1881 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3680 5392 96
Size: 274 80 33 51 1443 1703 1712 138
Fragments arranged by size:
1712 1703 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2391 2423 5394 5426 2391
Size: 32 2971 32 2399
Fragments arranged by size:
2971 2399 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5434
XbaI T'CTAG_A
Cuts at: 2770 2770
Size: 5434
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5434
XmaI C'CCGG_G
Cuts at: 1870 2759 1870
Size: 889 4545
XmnI GAAnn'nnTTC
Cuts at: 2495 3563 2495
Size: 1068 4366
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3242 276
Size: 53 83 186 936 1708 2468
Fragments arranged by size:
2468 1708 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspXI PsrI PvuII RsrII SacII
SfiI SfoI SrfI SwaI XcmI