Restriction map for
pFN21AB9691
(Circular) (Six-base) MAPSORT of: pFN21AB9691.seq Check: 9456 from: 1 to: 5395
pFN21AB9691 5395 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3205 278
Size: 53 83 186 936 1669 2468
Fragments arranged by size:
2468 1669 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2738 1937
Size: 801 4594
Acc65I G'GTAC_C
Cuts at: 2716 2716
Size: 5395
AclI AA'CG_TT
Cuts at: 1563 3522 3895 1563
Size: 1959 373 3063
Fragments arranged by size:
3063 1959 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2364 3458 4842 5110 1632
Size: 732 1094 1384 268 1917
Fragments arranged by size:
1917 1384 1094 732 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5198
AflIII A'CryG_T
Cuts at: 1203 4294 5271 1203
Size: 3091 977 1327
Fragments arranged by size:
3091 1327 977
AgeI A'CCGG_T
Cuts at: 4226 4226
Size: 5395
AhdI GACnn_n'nnGTC
Cuts at: 4124 4124
Size: 5395
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5395
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5395
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2432 2464 2432
Size: 32 5363
AlwNI CAG_nnn'CTG
Cuts at: 2387 2431 3138 4710 2387
Size: 44 707 1572 3072
Fragments arranged by size:
3072 1572 707 44
ApaLI G'TGCA_C
Cuts at: 3452 4608 3452
Size: 1156 4239
ApoI r'AATT_y
Cuts at: 1493 2704 2930 3082 5191 1493
Size: 1211 226 152 2109 1697
Fragments arranged by size:
2109 1697 1211 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3949 160
Size: 872 2917 1606
Fragments arranged by size:
2917 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5395
AvaI C'yCGr_G
Cuts at: 1870 1943 2273 2603 2720 1870
Size: 73 330 330 117 4545
Fragments arranged by size:
4545 330 330 117 73
BamHI G'GATC_C
Cuts at: 2725 3092 2725
Size: 367 5028
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2132 2138 2222 2300 2475
Size: 325 217 627 345 6 84 78 175
Cuts at: 2475 2609 2716 4171 618
Size: 134 107 1455 1842
Fragments arranged by size:
1842 1455 627 345 325 217 175 134
107 84 78 6
BanII G_rGCy'C
Cuts at: 1391 2198 2280 2714 1391
Size: 807 82 434 4072
Fragments arranged by size:
4072 807 434 82
BbeI G_GCGC'C
Cuts at: 2136 2142 2226 2304 2479 2613 2136
Size: 6 84 78 175 134 4918
Fragments arranged by size:
4918 175 134 84 78 6
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2370 2688 928
Size: 971 471 318 3635
Fragments arranged by size:
3635 971 471 318
BbvCI CC'TCA_GC
Cuts at: 2343 2343
Size: 5395
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2272 2306 2473 2507 3586 3620 1903
Size: 34 335 34 167 34 1079 34 3678
Fragments arranged by size:
3678 1079 335 167 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3287 4497 3287
Size: 1210 4185
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5395
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4006 136
Size: 107 122 71 631 605 2334 1525
Fragments arranged by size:
2334 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2529 5390 2529
Size: 2861 2534
BlpI GC'TnA_GC
Cuts at: 2812 2812
Size: 5395
Bme1580I G_kGCm'C
Cuts at: 1790 2619 3456 4612 1790
Size: 829 837 1156 2573
Fragments arranged by size:
2573 1156 837 829
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5395
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3126 4084 442
Size: 416 53 83 399 24 1709 958 1753
Fragments arranged by size:
1753 1709 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5395
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2453 2459 4055 1239
Size: 1214 6 1596 2579
Fragments arranged by size:
2579 1596 1214 6
Bpu10I CC'TnA_GC
Cuts at: 2343 2343
Size: 5395
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3520 4385 4683 3520
Size: 865 298 4232
Fragments arranged by size:
4232 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4058 882
Size: 617 2559 2219
Fragments arranged by size:
2559 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3186 493
Size: 711 1982 2702
Fragments arranged by size:
2702 1982 711
BsaBI GATnn'nnATC
Cuts at: 2528 5389 2528
Size: 2861 2534
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2133 2139 2223 2229
Size: 53 83 186 936 600 6 84 6
Cuts at: 2229 2301 2476 2610 3202 3584 4219 275
Size: 72 175 134 592 382 635 1451
Fragments arranged by size:
1451 936 635 600 592 382 186 175
134 84 83 72 53 6 6
BsaWI w'CCGG_w
Cuts at: 3828 4226 4500 4647 5058 3828
Size: 398 274 147 411 4165
Fragments arranged by size:
4165 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2432 2462 1178
Size: 30 165 30 1029 30 4111
Fragments arranged by size:
4111 1029 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2672 2702 1186
Size: 1486 30 3879
Fragments arranged by size:
3879 1486 30
BseYI C'CCAG_C
Cuts at: 4598 5094 4598
Size: 496 4899
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4822
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2154 2845 3606 4634 1540
Size: 275 186 153 691 761 1028 2301
Fragments arranged by size:
2301 1028 761 691 275 186 153
BsiHKAI G_wGCw'C
Cuts at: 3456 3541 4612 3456
Size: 85 1071 4239
Fragments arranged by size:
4239 1071 85
BsmI GAATG_Cn'
Cuts at: 2906 2999 2906
Size: 93 5302
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2198 2280 2619 2714 3456 3541 4612
Size: 399 408 82 339 95 742 85 1071
Cuts at: 4612 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 408 399 339 95 85
82
BspEI T'CCGG_A
Cuts at: 5058 5058
Size: 5395
BspHI T'CATG_A
Cuts at: 3284 5014 3284
Size: 1730 3665
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2250 2750 844
Size: 1406 500 3489
Fragments arranged by size:
3489 1406 500
BsrBI CCG'CTC
Cuts at: 1394 1823 3282 1394
Size: 429 1459 3507
Fragments arranged by size:
3507 1459 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3890 4064 65
Size: 1183 2642 174 1396
Fragments arranged by size:
2642 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2135 4039 4226 1700
Size: 110 325 1904 187 2869
Fragments arranged by size:
2869 1904 325 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5395
BssHII G'CGCG_C
Cuts at: 1925 2325 1925
Size: 400 4995
BssSI C'ACGA_G
Cuts at: 3455 4467 3455
Size: 1012 4383
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5395
BstBI TT'CG_AA
Cuts at: 4199 4199
Size: 5395
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5395
BstYI r'GATC_y
Cuts at: 1972 2529 2725 3092 3477 3494 4946 5032 5044
Size: 557 196 367 385 17 1452 86 12
Cuts at: 5044 5390 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 557 385 367 346 196 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2640 513
Size: 552 1575 3268
Fragments arranged by size:
3268 1575 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4656
Fragments arranged by size:
4656 683 56
BtsI GCAGTG_nn'
Cuts at: 2290 2631 2911 3705 3725 2290
Size: 341 280 794 20 3960
Fragments arranged by size:
3960 794 341 280 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5395
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5360
DraI TTT'AAA
Cuts at: 2699 2699
Size: 5395
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4402 809
Size: 507 3086 1802
Fragments arranged by size:
3086 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2842 3731 5360
Size: 54 1425 50 275 18 1012 889 1629
Cuts at: 5360 8
Size: 43
Fragments arranged by size:
1629 1425 1012 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2842 1537
Size: 275 1030 4090
Fragments arranged by size:
4090 1030 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2670 3325 1353
Size: 1317 655 3423
Fragments arranged by size:
3423 1317 655
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3982 4356 4502 387
Size: 1064 138 2393 374 146 1280
Fragments arranged by size:
2393 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2364 2453 2459 3458 4055 4842 5110
Size: 393 732 89 6 999 597 787 268
Cuts at: 5110 1239
Size: 1524
Fragments arranged by size:
1524 999 787 732 597 393 268 89
6
EcoO109I rG'GnC_Cy
Cuts at: 2384 2384
Size: 5395
EcoRI G'AATT_C
Cuts at: 2704 3082 2704
Size: 378 5017
FspI TGC'GCA
Cuts at: 3901 3901
Size: 5395
HaeII r_GCGC'y
Cuts at: 1678 2136 2142 2226 2304 2479 2521 2613 4542
Size: 458 6 84 78 175 42 92 1929
Cuts at: 4542 1678
Size: 2531
Fragments arranged by size:
2531 1929 458 175 92 84 78 42
6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4036 4068 4110 4142 1373
Size: 32 135 32 2464 32 42 32 2626
Fragments arranged by size:
2626 2464 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2176 2739 2985 669
Size: 988 281 238 563 246 3079
Fragments arranged by size:
3079 988 563 281 246 238
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5395
HpaI GTT'AAC
Cuts at: 2985 2985
Size: 5395
KasI G'GCGC_C
Cuts at: 2132 2138 2222 2300 2475 2609 2132
Size: 6 84 78 175 134 4918
Fragments arranged by size:
4918 175 134 84 78 6
KpnI G_GTAC'C
Cuts at: 2720 2720
Size: 5395
MfeI C'AATT_G
Cuts at: 2994 2994
Size: 5395
MluI A'CGCG_T
Cuts at: 5271 5271
Size: 5395
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2609 4509 4693 1305
Size: 1304 1900 184 2007
Fragments arranged by size:
2007 1900 1304 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3573
Fragments arranged by size:
3573 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3353 3712 3871 518
Size: 648 2187 359 159 2042
Fragments arranged by size:
2187 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2642 2811 3488 4636 4881 1606
Size: 1036 169 677 1148 245 2120
Fragments arranged by size:
2120 1148 1036 677 245 169
NaeI GCC'GGC
Cuts at: 2137 2137
Size: 5395
NarI GG'CG_CC
Cuts at: 2133 2139 2223 2301 2476 2610 2133
Size: 6 84 78 175 134 4918
Fragments arranged by size:
4918 175 134 84 78 6
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4843
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5395
NgoMIV G'CCGG_C
Cuts at: 2135 2135
Size: 5395
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5395
NotI GC'GGCC_GC
Cuts at: 2842 2842
Size: 5395
NspI r_CATG'y
Cuts at: 1132 1229 2753 4298 1132
Size: 97 1524 1545 2229
Fragments arranged by size:
2229 1545 1524 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5395
PciI A'CATG_T
Cuts at: 4294 4294
Size: 5395
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2431 1108
Size: 1323 4072
PmeI GTTT'AAAC
Cuts at: 2699 2699
Size: 5395
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2432 2464 3464 3496 4977 5009 2432
Size: 32 1000 32 1481 32 2818
Fragments arranged by size:
2818 1481 1000 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2384 2384
Size: 5395
PshAI GACnn'nnGTC
Cuts at: 1613 2580 1613
Size: 967 4428
PsiI TTA'TAA
Cuts at: 2965 2965
Size: 5395
PstI C_TGCA'G
Cuts at: 830 2285 2747 830
Size: 1455 462 3478
Fragments arranged by size:
3478 1455 462
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5395
SacII CC_GC'GG
Cuts at: 2643 2643
Size: 5395
SalI G'TCGA_C
Cuts at: 1936 2737 1936
Size: 801 4594
SbfI CC_TGCA'GG
Cuts at: 2747 2747
Size: 5395
ScaI AGT'ACT
Cuts at: 3643 5355 3643
Size: 1712 3683
SfcI C'TryA_G
Cuts at: 826 1045 2281 2743 3878 4559 4750 826
Size: 219 1236 462 1135 681 191 1471
Fragments arranged by size:
1471 1236 1135 681 462 219 191
SfoI GGC'GCC
Cuts at: 2134 2140 2224 2302 2477 2611 2134
Size: 6 84 78 175 134 4918
Fragments arranged by size:
4918 175 134 84 78 6
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5395
SgrAI Cr'CCGG_yG
Cuts at: 1700 2135 1700
Size: 435 4960
SmaI CCC'GGG
Cuts at: 1872 2275 2722 1872
Size: 403 447 4545
Fragments arranged by size:
4545 447 403
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3499 4400 4662 820
Size: 197 926 1556 901 262 1553
Fragments arranged by size:
1556 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3186 493
Size: 2693 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5395
SphI G_CATG'C
Cuts at: 1132 2753 1132
Size: 1621 3774
SspI AAT'ATT
Cuts at: 5 52 3319 5
Size: 47 3267 2081
Fragments arranged by size:
3267 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5395
StyI C'CwwG_G
Cuts at: 513 1065 2834 513
Size: 552 1769 3074
Fragments arranged by size:
3074 1769 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3422 3439 3592 3777 4222 325
Size: 1255 1842 17 153 185 445 1498
Fragments arranged by size:
1842 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3641 5353 96
Size: 274 80 33 51 1443 1664 1712 138
Fragments arranged by size:
1712 1664 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5355 5387 5355
Size: 32 5363
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5395
XbaI T'CTAG_A
Cuts at: 2731 2731
Size: 5395
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5395
XmaI C'CCGG_G
Cuts at: 1870 2273 2720 1870
Size: 403 447 4545
Fragments arranged by size:
4545 447 403
XmnI GAAnn'nnTTC
Cuts at: 2447 3524 2447
Size: 1077 4318
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3203 276
Size: 53 83 186 936 1669 2468
Fragments arranged by size:
2468 1669 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI SacII SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI NruI NsiI PacI PfoI
PmlI PspOMI PspXI PsrI PvuII RsrII SacI SanDI
SapI SexAI SfiI SrfI SwaI XcmI