Restriction map for
pFN21AB9690
(Circular) (Six-base) MAPSORT of: pFN21AB9690.seq Check: 5834 from: 1 to: 5383
pFN21AB9690 5383 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2082 3193 278
Size: 53 83 186 936 546 1111 2468
Fragments arranged by size:
2468 1111 936 546 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2726 1937
Size: 789 4594
Acc65I G'GTAC_C
Cuts at: 2704 2704
Size: 5383
AclI AA'CG_TT
Cuts at: 1563 3510 3883 1563
Size: 1947 373 3063
Fragments arranged by size:
3063 1947 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2429 3446 4830 5098 1632
Size: 797 1017 1384 268 1917
Fragments arranged by size:
1917 1384 1017 797 268
AfeI AGC'GCT
Cuts at: 2315 2423 2315
Size: 108 5275
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5186
AflIII A'CryG_T
Cuts at: 1203 4282 5259 1203
Size: 3079 977 1327
Fragments arranged by size:
3079 1327 977
AgeI A'CCGG_T
Cuts at: 2300 4214 2300
Size: 1914 3469
AhdI GACnn_n'nnGTC
Cuts at: 4112 4112
Size: 5383
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5383
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5383
AlwNI CAG_nnn'CTG
Cuts at: 3126 4698 3126
Size: 1572 3811
ApaI G_GGCC'C
Cuts at: 2115 2115
Size: 5383
ApaLI G'TGCA_C
Cuts at: 3440 4596 3440
Size: 1156 4227
ApoI r'AATT_y
Cuts at: 1493 2692 2918 3070 5179 1493
Size: 1199 226 152 2109 1697
Fragments arranged by size:
2109 1697 1199 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3937 160
Size: 872 2905 1606
Fragments arranged by size:
2905 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5383
AvaI C'yCGr_G
Cuts at: 1870 1943 2108 2219 2307 2708 1870
Size: 73 165 111 88 401 4545
Fragments arranged by size:
4545 401 165 111 88 73
BamHI G'GATC_C
Cuts at: 2713 3080 2713
Size: 367 5016
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2089 2297 2704 4159 618
Size: 325 217 627 302 208 407 1455 1842
Fragments arranged by size:
1842 1455 627 407 325 302 217 208
BanII G_rGCy'C
Cuts at: 1391 2115 2602 2702 1391
Size: 724 487 100 4072
Fragments arranged by size:
4072 724 487 100
BbeI G_GCGC'C
Cuts at: 2093 2093
Size: 5383
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2181 928
Size: 971 282 4130
Fragments arranged by size:
4130 971 282
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2298 2332 3574 3608 1903
Size: 34 361 34 1242 34 3678
Fragments arranged by size:
3678 1242 361 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3275 4485 3275
Size: 1210 4173
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5383
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2110 2412 2501
Size: 107 122 71 631 605 438 302 89
Cuts at: 2501 3994 136
Size: 1493 1525
Fragments arranged by size:
1525 1493 631 605 438 302 122 107
89 71
BglII A'GATC_T
Cuts at: 5378 5378
Size: 5383
BlpI GC'TnA_GC
Cuts at: 2354 2800 2354
Size: 446 4937
Bme1580I G_kGCm'C
Cuts at: 1790 2115 3444 4600 1790
Size: 325 1329 1156 2573
Fragments arranged by size:
2573 1329 1156 325
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5383
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2050 2598 3114
Size: 416 53 83 399 24 633 548 516
Cuts at: 3114 4072 442
Size: 958 1753
Fragments arranged by size:
1753 958 633 548 516 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5383
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2084 4043 1239
Size: 845 1959 2579
Fragments arranged by size:
2579 1959 845
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3508 4373 4671 3508
Size: 865 298 4220
Fragments arranged by size:
4220 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4046 882
Size: 617 2547 2219
Fragments arranged by size:
2547 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3174 493
Size: 711 1970 2702
Fragments arranged by size:
2702 1970 711
BsaBI GATnn'nnATC
Cuts at: 5377 5377
Size: 5383
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2079 2090 3190 3572
Size: 53 83 186 936 546 11 1100 382
Cuts at: 3572 4207 275
Size: 635 1451
Fragments arranged by size:
1451 1100 936 635 546 382 186 83
53 11
BsaWI w'CCGG_w
Cuts at: 2300 2508 3816 4214 4488 4635 5046 2300
Size: 208 1308 398 274 147 411 2637
Fragments arranged by size:
2637 1308 411 398 274 208 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5158
Fragments arranged by size:
5158 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2216 1186
Size: 1030 4353
BseYI C'CCAG_C
Cuts at: 2114 4586 5082 2114
Size: 2472 496 2415
Fragments arranged by size:
2472 2415 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2302 1163
Size: 573 566 4244
Fragments arranged by size:
4244 573 566
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2034 2833 3594 4622 1540
Size: 275 186 33 799 761 1028 2301
Fragments arranged by size:
2301 1028 799 761 275 186 33
BsiHKAI G_wGCw'C
Cuts at: 3444 3529 4600 3444
Size: 85 1071 4227
Fragments arranged by size:
4227 1071 85
BsmI GAATG_Cn'
Cuts at: 2894 2987 2894
Size: 93 5290
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2115 2602 2702 3444 3529 4600 1391
Size: 399 325 487 100 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 487 399 325 100 85
BspEI T'CCGG_A
Cuts at: 2508 5046 2508
Size: 2538 2845
BspHI T'CATG_A
Cuts at: 3272 5002 3272
Size: 1730 3653
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2738 844
Size: 1894 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2272 2476 3270 1394
Size: 429 449 204 794 3507
Fragments arranged by size:
3507 794 449 429 204
BsrDI GCAATG_nn'
Cuts at: 65 1248 3878 4052 65
Size: 1183 2630 174 1396
Fragments arranged by size:
2630 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2125 2294 2300 4027 4214 1700
Size: 110 315 169 6 1727 187 2869
Fragments arranged by size:
2869 1727 315 187 169 110 6
BsrGI T'GTAC_A
Cuts at: 96 2664 96
Size: 2568 2815
BssHII G'CGCG_C
Cuts at: 1925 2373 2375 1925
Size: 448 2 4933
Fragments arranged by size:
4933 448 2
BssSI C'ACGA_G
Cuts at: 3443 4455 3443
Size: 1012 4371
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5383
BstBI TT'CG_AA
Cuts at: 4187 4187
Size: 5383
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5383
BstXI CCAn_nnnn'nTGG
Cuts at: 2611 2611
Size: 5383
BstYI r'GATC_y
Cuts at: 1972 2713 3080 3465 3482 4934 5020 5032 5378
Size: 741 367 385 17 1452 86 12 346
Cuts at: 5378 1972
Size: 1977
Fragments arranged by size:
1977 1452 741 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2280 2280
Size: 5383
BtgI C'CryG_G
Cuts at: 513 1065 2004 2028 2093 2325 2389 513
Size: 552 939 24 65 232 64 3507
Fragments arranged by size:
3507 939 552 232 65 64 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4644
Fragments arranged by size:
4644 683 56
BtsI GCAGTG_nn'
Cuts at: 2899 3693 3713 2899
Size: 794 20 4569
Fragments arranged by size:
4569 794 20
ClaI AT'CG_AT
Cuts at: 1554 2049 1554
Size: 495 4888
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5348
DraI TTT'AAA
Cuts at: 2687 2687
Size: 5383
DraIII CAC_nnn'GTG
Cuts at: 2395 2395
Size: 5383
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4390 809
Size: 507 3074 1802
Fragments arranged by size:
3074 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2031 2230
Size: 54 1425 50 275 18 177 24 199
Cuts at: 2230 2490 2613 2830 3719 5348 8
Size: 260 123 217 889 1629 43
Fragments arranged by size:
1629 1425 889 275 260 217 199 177
123 54 50 43 24 18
EagI C'GGCC_G
Cuts at: 1537 1812 2031 2830 1537
Size: 275 219 799 4090
Fragments arranged by size:
4090 799 275 219
EarI CTCTTCn'nnn_
Cuts at: 1353 2067 2181 3313 1353
Size: 714 114 1132 3423
Fragments arranged by size:
3423 1132 714 114
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2144 3970 4344 4490 387
Size: 1064 138 555 1826 374 146 1280
Fragments arranged by size:
1826 1280 1064 555 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2084 2429 3446 4043 4830 5098 1239
Size: 393 452 345 1017 597 787 268 1524
Fragments arranged by size:
1524 1017 787 597 452 393 345 268
EcoRI G'AATT_C
Cuts at: 2692 3070 2692
Size: 378 5005
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2654 2654
Size: 5383
FspI TGC'GCA
Cuts at: 3889 3889
Size: 5383
HaeII r_GCGC'y
Cuts at: 1678 2093 2317 2425 2507 4530 1678
Size: 415 224 108 82 2023 2531
Fragments arranged by size:
2531 2023 415 224 108 82
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4024 4056 4098 4130 1373
Size: 32 135 32 2452 32 42 32 2626
Fragments arranged by size:
2626 2452 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2727 2973 669
Size: 988 281 789 246 3079
Fragments arranged by size:
3079 988 789 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5383
HpaI GTT'AAC
Cuts at: 2973 2973
Size: 5383
KasI G'GCGC_C
Cuts at: 2089 2089
Size: 5383
KpnI G_GTAC'C
Cuts at: 2708 2708
Size: 5383
MfeI C'AATT_G
Cuts at: 2982 2982
Size: 5383
MluI A'CGCG_T
Cuts at: 5259 5259
Size: 5383
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4497 4681 1305
Size: 3192 184 2007
Fragments arranged by size:
3192 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2615 10
Size: 54 1425 343 783 2778
Fragments arranged by size:
2778 1425 783 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3341 3700 3859 518
Size: 648 2175 359 159 2042
Fragments arranged by size:
2175 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2030 2095 2245 2327 2799 3476 4624 4869
Size: 424 65 150 82 472 677 1148 245
Cuts at: 4869 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 472 424 245 150 82
65
NaeI GCC'GGC
Cuts at: 2127 2296 2127
Size: 169 5214
NarI GG'CG_CC
Cuts at: 2090 2090
Size: 5383
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3892
Fragments arranged by size:
3892 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5383
NgoMIV G'CCGG_C
Cuts at: 2125 2294 2125
Size: 169 5214
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5383
NotI GC'GGCC_GC
Cuts at: 2830 2830
Size: 5383
NspI r_CATG'y
Cuts at: 1132 1229 2741 4286 1132
Size: 97 1512 1545 2229
Fragments arranged by size:
2229 1545 1512 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5383
PciI A'CATG_T
Cuts at: 4282 4282
Size: 5383
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2395 1108
Size: 1287 4096
PfoI T'CCnGG_A
Cuts at: 2546 2546
Size: 5383
PmeI GTTT'AAAC
Cuts at: 2687 2687
Size: 5383
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3452 3484 4965 4997 3452
Size: 32 1481 32 3838
Fragments arranged by size:
3838 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5383
PsiI TTA'TAA
Cuts at: 2953 2953
Size: 5383
PspOMI G'GGCC_C
Cuts at: 2111 2111
Size: 5383
PstI C_TGCA'G
Cuts at: 830 2735 830
Size: 1905 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5383
SacII CC_GC'GG
Cuts at: 2031 2096 2328 2031
Size: 65 232 5086
Fragments arranged by size:
5086 232 65
SalI G'TCGA_C
Cuts at: 1936 2725 1936
Size: 789 4594
SbfI CC_TGCA'GG
Cuts at: 2735 2735
Size: 5383
ScaI AGT'ACT
Cuts at: 3631 5343 3631
Size: 1712 3671
SfcI C'TryA_G
Cuts at: 826 1045 2650 2731 3866 4547 4738 826
Size: 219 1605 81 1135 681 191 1471
Fragments arranged by size:
1605 1471 1135 681 219 191 81
SfoI GGC'GCC
Cuts at: 2091 2091
Size: 5383
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5383
SgrAI Cr'CCGG_yG
Cuts at: 1700 2125 1700
Size: 425 4958
SmaI CCC'GGG
Cuts at: 1872 2110 2221 2710 1872
Size: 238 111 489 4545
Fragments arranged by size:
4545 489 238 111
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3487 4388 4650 820
Size: 197 926 1544 901 262 1553
Fragments arranged by size:
1553 1544 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3174 493
Size: 2681 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5383
SphI G_CATG'C
Cuts at: 1132 2741 1132
Size: 1609 3774
SspI AAT'ATT
Cuts at: 5 52 3307 5
Size: 47 3255 2081
Fragments arranged by size:
3255 2081 47
StuI AGG'CCT
Cuts at: 1517 2285 1517
Size: 768 4615
StyI C'CwwG_G
Cuts at: 513 1065 2004 2638 2822 513
Size: 552 939 634 184 3074
Fragments arranged by size:
3074 939 634 552 184
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3410 3427 3580 3765 4210 325
Size: 1255 1830 17 153 185 445 1498
Fragments arranged by size:
1830 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2664 3629 5341
Size: 274 80 33 51 1443 687 965 1712
Cuts at: 5341 96
Size: 138
Fragments arranged by size:
1712 1443 965 687 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5343 5375 5343
Size: 32 5351
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5383
XbaI T'CTAG_A
Cuts at: 2719 2719
Size: 5383
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2461 2461
Size: 5383
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5383
XmaI C'CCGG_G
Cuts at: 1870 2108 2219 2708 1870
Size: 238 111 489 4545
Fragments arranged by size:
4545 489 238 111
XmnI GAAnn'nnTTC
Cuts at: 2561 3512 2561
Size: 951 4432
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2080 3191 276
Size: 53 83 186 936 546 1111 2468
Fragments arranged by size:
2468 1111 936 546 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstZ17I EcoICRI EcoNI EcoO109I EcoRV
FseI FspAI NruI NsiI PacI PmlI PpuMI PspXI
PsrI PvuII RsrII SacI SanDI SapI SexAI SfiI
SrfI SwaI