Restriction map for
pFN21AB9689
(Circular) (Six-base) MAPSORT of: pFN21AB9689.seq Check: 9613 from: 1 to: 5395
pFN21AB9689 5395 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3205 278
Size: 53 83 186 936 1669 2468
Fragments arranged by size:
2468 1669 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2738 1937
Size: 801 4594
Acc65I G'GTAC_C
Cuts at: 2716 2716
Size: 5395
AclI AA'CG_TT
Cuts at: 1563 3522 3895 1563
Size: 1959 373 3063
Fragments arranged by size:
3063 1959 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3458 4842 5110 1632
Size: 1826 1384 268 1917
Fragments arranged by size:
1917 1826 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5198
AflIII A'CryG_T
Cuts at: 1203 4294 5271 1203
Size: 3091 977 1327
Fragments arranged by size:
3091 1327 977
AgeI A'CCGG_T
Cuts at: 4226 4226
Size: 5395
AhdI GACnn_n'nnGTC
Cuts at: 4124 4124
Size: 5395
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5395
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5395
AlwNI CAG_nnn'CTG
Cuts at: 3138 4710 3138
Size: 1572 3823
ApaLI G'TGCA_C
Cuts at: 2510 3452 4608 2510
Size: 942 1156 3297
Fragments arranged by size:
3297 1156 942
ApoI r'AATT_y
Cuts at: 1493 2704 2930 3082 5191 1493
Size: 1211 226 152 2109 1697
Fragments arranged by size:
2109 1697 1211 226 152
AscI GG'CGCG_CC
Cuts at: 2583 2583
Size: 5395
AseI AT'TA_AT
Cuts at: 160 1032 3949 160
Size: 872 2917 1606
Fragments arranged by size:
2917 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5395
AvaI C'yCGr_G
Cuts at: 1870 1943 2013 2133 2720 1870
Size: 73 70 120 587 4545
Fragments arranged by size:
4545 587 120 73 70
BamHI G'GATC_C
Cuts at: 2725 3092 2725
Size: 367 5028
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2073 2590 2644 2716 4171
Size: 325 217 627 286 517 54 72 1455
Cuts at: 4171 618
Size: 1842
Fragments arranged by size:
1842 1455 627 517 325 286 217 72
54
BanII G_rGCy'C
Cuts at: 1391 2226 2714 1391
Size: 835 488 4072
Fragments arranged by size:
4072 835 488
BbeI G_GCGC'C
Cuts at: 2077 2594 2648 2077
Size: 517 54 4824
Fragments arranged by size:
4824 517 54
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4424
BbvCI CC'TCA_GC
Cuts at: 2108 2108
Size: 5395
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3586 3620 1903
Size: 34 1649 34 3678
Fragments arranged by size:
3678 1649 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3287 4497 3287
Size: 1210 4185
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5395
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2237 2354 2474
Size: 107 122 71 631 605 565 117 120
Cuts at: 2474 4006 136
Size: 1532 1525
Fragments arranged by size:
1532 1525 631 605 565 122 120 117
107 71
BglII A'GATC_T
Cuts at: 5390 5390
Size: 5395
BlpI GC'TnA_GC
Cuts at: 2812 2812
Size: 5395
Bme1580I G_kGCm'C
Cuts at: 1790 2514 3456 4612 1790
Size: 724 942 1156 2573
Fragments arranged by size:
2573 1156 942 724
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5395
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2255 2501 3126
Size: 416 53 83 399 24 838 246 625
Cuts at: 3126 4084 442
Size: 958 1753
Fragments arranged by size:
1753 958 838 625 416 399 246 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5395
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2144 2183 2226 2424 2701 4055 1239
Size: 905 39 43 198 277 1354 2579
Fragments arranged by size:
2579 1354 905 277 198 43 39
Bpu10I CC'TnA_GC
Cuts at: 2108 2108
Size: 5395
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2036 2221 3520 4385 4683 2036
Size: 185 1299 865 298 2748
Fragments arranged by size:
2748 1299 865 298 185
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2677 4058 882
Size: 617 1178 1381 2219
Fragments arranged by size:
2219 1381 1178 617
BsaAI yAC'GTr
Cuts at: 493 1204 3186 493
Size: 711 1982 2702
Fragments arranged by size:
2702 1982 711
BsaBI GATnn'nnATC
Cuts at: 5389 5389
Size: 5395
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2074 2271 2346 2591
Size: 53 83 186 936 541 197 75 245
Cuts at: 2591 2645 3202 3584 4219 275
Size: 54 557 382 635 1451
Fragments arranged by size:
1451 936 635 557 541 382 245 197
186 83 75 54 53
BsaWI w'CCGG_w
Cuts at: 2148 2300 2600 3828 4226 4500 4647 5058 2148
Size: 152 300 1228 398 274 147 411 2485
Fragments arranged by size:
2485 1228 411 398 300 274 152 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5170
Fragments arranged by size:
5170 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5395
BseYI C'CCAG_C
Cuts at: 2367 4598 5094 2367
Size: 2231 496 2668
Fragments arranged by size:
2668 2231 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2369 2477 1163
Size: 573 633 108 4081
Fragments arranged by size:
4081 633 573 108
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2232 2358 2845 3606 4634 1540
Size: 275 186 231 126 487 761 1028 2301
Fragments arranged by size:
2301 1028 761 487 275 231 186 126
BsiHKAI G_wGCw'C
Cuts at: 2514 3456 3541 4612 2514
Size: 942 85 1071 3297
Fragments arranged by size:
3297 1071 942 85
BsmI GAATG_Cn'
Cuts at: 2906 2999 2906
Size: 93 5302
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2226 2514 2714 3456 3541 4612 1391
Size: 399 436 288 200 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 436 399 288 200 85
BspEI T'CCGG_A
Cuts at: 2148 5058 2148
Size: 2910 2485
BspHI T'CATG_A
Cuts at: 3284 5014 3284
Size: 1730 3665
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2750 844
Size: 1906 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2128 3282 1394
Size: 429 305 1154 3507
Fragments arranged by size:
3507 1154 429 305
BsrDI GCAATG_nn'
Cuts at: 65 1248 3890 4064 65
Size: 1183 2642 174 1396
Fragments arranged by size:
2642 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2422 2473 2587 4039 4226 1700
Size: 110 612 51 114 1452 187 2869
Fragments arranged by size:
2869 1452 612 187 114 110 51
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5395
BssHII G'CGCG_C
Cuts at: 1925 2566 2583 1925
Size: 641 17 4737
Fragments arranged by size:
4737 641 17
BssSI C'ACGA_G
Cuts at: 3455 4467 3455
Size: 1012 4383
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5395
BstBI TT'CG_AA
Cuts at: 4199 4199
Size: 5395
BstEII G'GTnAC_C
Cuts at: 1184 2008 1184
Size: 824 4571
BstYI r'GATC_y
Cuts at: 1972 2428 2725 3092 3477 3494 4946 5032 5044
Size: 456 297 367 385 17 1452 86 12
Cuts at: 5044 5390 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 456 385 367 346 297 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2190 2235 513
Size: 552 939 186 45 3673
Fragments arranged by size:
3673 939 552 186 45
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4656
Fragments arranged by size:
4656 683 56
BtsI GCAGTG_nn'
Cuts at: 2911 3705 3725 2911
Size: 794 20 4581
Fragments arranged by size:
4581 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5395
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5360
DraI TTT'AAA
Cuts at: 2699 2699
Size: 5395
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4402 809
Size: 507 3086 1802
Fragments arranged by size:
3086 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2229 2355 2842
Size: 54 1425 50 275 18 399 126 487
Cuts at: 2842 3731 5360 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 487 399 275 126 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2229 2355 2842 1537
Size: 275 417 126 487 4090
Fragments arranged by size:
4090 487 417 275 126
EarI CTCTTCn'nnn_
Cuts at: 1353 2097 2378 3325 1353
Size: 744 281 947 3423
Fragments arranged by size:
3423 947 744 281
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3982 4356 4502 387
Size: 1064 138 2393 374 146 1280
Fragments arranged by size:
2393 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2144 2183 2226 2424 2701 3458 4055
Size: 393 512 39 43 198 277 757 597
Cuts at: 4055 4842 5110 1239
Size: 787 268 1524
Fragments arranged by size:
1524 787 757 597 512 393 277 268
198 43 39
EcoO109I rG'GnC_Cy
Cuts at: 2653 2653
Size: 5395
EcoRI G'AATT_C
Cuts at: 2704 3082 2704
Size: 378 5017
FspI TGC'GCA
Cuts at: 2539 3901 2539
Size: 1362 4033
HaeII r_GCGC'y
Cuts at: 1678 2077 2282 2447 2503 2594 2648 4542 1678
Size: 399 205 165 56 91 54 1894 2531
Fragments arranged by size:
2531 1894 399 205 165 91 56 54
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4036 4068 4110 4142 1373
Size: 32 135 32 2464 32 42 32 2626
Fragments arranged by size:
2626 2464 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2739 2985 669
Size: 988 281 801 246 3079
Fragments arranged by size:
3079 988 801 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5395
HpaI GTT'AAC
Cuts at: 2985 2985
Size: 5395
KasI G'GCGC_C
Cuts at: 2073 2590 2644 2073
Size: 517 54 4824
Fragments arranged by size:
4824 517 54
KpnI G_GTAC'C
Cuts at: 2720 2720
Size: 5395
MfeI C'AATT_G
Cuts at: 2994 2994
Size: 5395
MluI A'CGCG_T
Cuts at: 5271 5271
Size: 5395
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4509 4693 1305
Size: 3204 184 2007
Fragments arranged by size:
3204 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3573
Fragments arranged by size:
3573 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3353 3712 3871 518
Size: 648 2187 359 159 2042
Fragments arranged by size:
2187 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2237 2289 2659 2811 3488 4636 4881 1606
Size: 631 52 370 152 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 631 370 245 152 52
NaeI GCC'GGC
Cuts at: 2589 2589
Size: 5395
NarI GG'CG_CC
Cuts at: 2074 2591 2645 2074
Size: 517 54 4824
Fragments arranged by size:
4824 517 54
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3904
Fragments arranged by size:
3904 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5395
NgoMIV G'CCGG_C
Cuts at: 2587 2587
Size: 5395
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5395
NotI GC'GGCC_GC
Cuts at: 2842 2842
Size: 5395
NspI r_CATG'y
Cuts at: 1132 1229 2753 4298 1132
Size: 97 1524 1545 2229
Fragments arranged by size:
2229 1545 1524 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5395
PciI A'CATG_T
Cuts at: 4294 4294
Size: 5395
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5395
PfoI T'CCnGG_A
Cuts at: 2265 2265
Size: 5395
PmeI GTTT'AAAC
Cuts at: 2699 2699
Size: 5395
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3464 3496 4977 5009 3464
Size: 32 1481 32 3850
Fragments arranged by size:
3850 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5395
PsiI TTA'TAA
Cuts at: 2965 2965
Size: 5395
PstI C_TGCA'G
Cuts at: 830 2747 830
Size: 1917 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5395
PvuII CAG'CTG
Cuts at: 2289 2289
Size: 5395
SacII CC_GC'GG
Cuts at: 2238 2238
Size: 5395
SalI G'TCGA_C
Cuts at: 1936 2737 1936
Size: 801 4594
SapI GCTCTTCn'nnn_
Cuts at: 2097 2378 2097
Size: 281 5114
SbfI CC_TGCA'GG
Cuts at: 2747 2747
Size: 5395
ScaI AGT'ACT
Cuts at: 3643 5355 3643
Size: 1712 3683
SfcI C'TryA_G
Cuts at: 826 1045 2743 3878 4559 4750 826
Size: 219 1698 1135 681 191 1471
Fragments arranged by size:
1698 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2075 2592 2646 2075
Size: 517 54 4824
Fragments arranged by size:
4824 517 54
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5395
SgrAI Cr'CCGG_yG
Cuts at: 1700 2587 1700
Size: 887 4508
SmaI CCC'GGG
Cuts at: 1872 2015 2135 2722 1872
Size: 143 120 587 4545
Fragments arranged by size:
4545 587 143 120
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2051 2200 3499 4400 4662 820
Size: 197 926 108 149 1299 901 262 1553
Fragments arranged by size:
1553 1299 926 901 262 197 149 108
SnaBI TAC'GTA
Cuts at: 493 3186 493
Size: 2693 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5395
SphI G_CATG'C
Cuts at: 1132 2753 1132
Size: 1621 3774
SspI AAT'ATT
Cuts at: 5 52 3319 5
Size: 47 3267 2081
Fragments arranged by size:
3267 2081 47
StuI AGG'CCT
Cuts at: 1517 2437 1517
Size: 920 4475
StyI C'CwwG_G
Cuts at: 513 1065 2004 2479 2649 2687 2834 513
Size: 552 939 475 170 38 147 3074
Fragments arranged by size:
3074 939 552 475 170 147 38
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3422 3439 3592 3777 4222 325
Size: 1255 1842 17 153 185 445 1498
Fragments arranged by size:
1842 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3641 5353 96
Size: 274 80 33 51 1443 1664 1712 138
Fragments arranged by size:
1712 1664 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5355 5387 5355
Size: 32 5363
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5395
XbaI T'CTAG_A
Cuts at: 2731 2731
Size: 5395
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5395
XmaI C'CCGG_G
Cuts at: 1870 2013 2133 2720 1870
Size: 143 120 587 4545
Fragments arranged by size:
4545 587 143 120
XmnI GAAnn'nnTTC
Cuts at: 3524 3524
Size: 5395
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3203 276
Size: 53 83 186 936 1669 2468
Fragments arranged by size:
2468 1669 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AscI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SacII SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AvrII BaeI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI NruI NsiI PacI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacI SanDI SexAI
SfiI SrfI SwaI XcmI