Restriction map for
pFN21AB9672
(Circular) (Six-base) MAPSORT of: pFN21AB9672.seq Check: 5574 from: 1 to: 5977
pFN21AB9672 5977 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3787 278
Size: 53 83 186 936 2251 2468
Fragments arranged by size:
2468 2251 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2567 2621 2628 3271 3320 1937
Size: 630 54 7 643 49 4594
Fragments arranged by size:
4594 643 630 54 49 7
Acc65I G'GTAC_C
Cuts at: 3298 3298
Size: 5977
AclI AA'CG_TT
Cuts at: 1563 4104 4477 1563
Size: 2541 373 3063
Fragments arranged by size:
3063 2541 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2318 2820 2865 3095 4040 5424 5692 1632
Size: 686 502 45 230 945 1384 268 1917
Fragments arranged by size:
1917 1384 945 686 502 268 230 45
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5780
AflIII A'CryG_T
Cuts at: 1203 2209 4876 5853 1203
Size: 1006 2667 977 1327
Fragments arranged by size:
2667 1327 1006 977
AgeI A'CCGG_T
Cuts at: 4808 4808
Size: 5977
AhdI GACnn_n'nnGTC
Cuts at: 4706 4706
Size: 5977
AleI CACnn'nnGTG
Cuts at: 1166 3162 1166
Size: 1996 3981
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2576 3204 1184
Size: 1392 628 3957
Fragments arranged by size:
3957 1392 628
AlwNI CAG_nnn'CTG
Cuts at: 2881 3720 5292 2881
Size: 839 1572 3566
Fragments arranged by size:
3566 1572 839
ApaLI G'TGCA_C
Cuts at: 2359 4034 5190 2359
Size: 1675 1156 3146
Fragments arranged by size:
3146 1675 1156
ApoI r'AATT_y
Cuts at: 1493 2092 2470 2499 2652 3286 3512 3664 5773
Size: 599 378 29 153 634 226 152 2109
Cuts at: 5773 1493
Size: 1697
Fragments arranged by size:
2109 1697 634 599 378 226 153 152
29
AseI AT'TA_AT
Cuts at: 160 1032 2121 4531 160
Size: 872 1089 2410 1606
Fragments arranged by size:
2410 1606 1089 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5977
AvaI C'yCGr_G
Cuts at: 1870 1943 2925 3302 1870
Size: 73 982 377 4545
Fragments arranged by size:
4545 982 377 73
BamHI G'GATC_C
Cuts at: 3307 3674 3307
Size: 367 5610
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2904 3298 4753 618
Size: 325 217 627 1117 394 1455 1842
Fragments arranged by size:
1842 1455 1117 627 394 325 217
BanII G_rGCy'C
Cuts at: 1391 3296 1391
Size: 1905 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2430 3092 928
Size: 971 531 662 3813
Fragments arranged by size:
3813 971 662 531
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4168 4202 1903
Size: 34 2231 34 3678
Fragments arranged by size:
3678 2231 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2669 3869 5079 2669
Size: 1200 1210 3567
Fragments arranged by size:
3567 1210 1200
BclI T'GATC_A
Cuts at: 1548 2769 1548
Size: 1221 4756
BfrBI ATG'CAT
Cuts at: 2971 2971
Size: 5977
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4588 136
Size: 107 122 71 631 605 2916 1525
Fragments arranged by size:
2916 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5972 5972
Size: 5977
BlpI GC'TnA_GC
Cuts at: 3394 3394
Size: 5977
Bme1580I G_kGCm'C
Cuts at: 1790 2145 2363 2915 4038 5194 1790
Size: 355 218 552 1123 1156 2573
Fragments arranged by size:
2573 1156 1123 552 355 218
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5977
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3708 4666 442
Size: 416 53 83 399 24 2291 958 1753
Fragments arranged by size:
2291 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5977
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2295 2903 4637 1239
Size: 1056 608 1734 2579
Fragments arranged by size:
2579 1734 1056 608
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4102 4967 5265 4102
Size: 865 298 4814
Fragments arranged by size:
4814 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4640 882
Size: 617 3141 2219
Fragments arranged by size:
3141 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3768 493
Size: 711 2564 2702
Fragments arranged by size:
2702 2564 711
BsaBI GATnn'nnATC
Cuts at: 5971 5971
Size: 5977
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3784 4166 4801 275
Size: 53 83 186 936 2251 382 635 1451
Fragments arranged by size:
2251 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4410 4808 5082 5229 5640 4410
Size: 398 274 147 411 4747
Fragments arranged by size:
4747 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2043 2073 1178
Size: 30 165 30 640 30 5082
Fragments arranged by size:
5082 640 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5977
BseYI C'CCAG_C
Cuts at: 5180 5676 5180
Size: 496 5481
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2256 1163
Size: 573 520 4884
Fragments arranged by size:
4884 573 520
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3427 4188 5216 1540
Size: 275 186 1426 761 1028 2301
Fragments arranged by size:
2301 1426 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2281 2363 4038 4123 5194 2281
Size: 82 1675 85 1071 3064
Fragments arranged by size:
3064 1675 1071 85 82
BsmI GAATG_Cn'
Cuts at: 2761 3488 3581 2761
Size: 727 93 5157
Fragments arranged by size:
5157 727 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2145 2281 2363 2915 3296 4038 4123
Size: 399 355 136 82 552 381 742 85
Cuts at: 4123 5194 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 552 399 381 355 136
85 82
BspEI T'CCGG_A
Cuts at: 5640 5640
Size: 5977
BspHI T'CATG_A
Cuts at: 3866 5596 3866
Size: 1730 4247
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3332 844
Size: 2488 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3864 1394
Size: 429 2041 3507
Fragments arranged by size:
3507 2041 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2560 4472 4646 65
Size: 1183 1312 1912 174 1396
Fragments arranged by size:
1912 1396 1312 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4621 4808 1700
Size: 110 2811 187 2869
Fragments arranged by size:
2869 2811 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5977
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5977
BssSI C'ACGA_G
Cuts at: 4037 5049 4037
Size: 1012 4965
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5977
BstBI TT'CG_AA
Cuts at: 4781 4781
Size: 5977
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5977
BstYI r'GATC_y
Cuts at: 1972 3307 3674 4059 4076 5528 5614 5626 5972
Size: 1335 367 385 17 1452 86 12 346
Cuts at: 5972 1972
Size: 1977
Fragments arranged by size:
1977 1452 1335 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3217 513
Size: 552 939 1213 3273
Fragments arranged by size:
3273 1213 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5238
Fragments arranged by size:
5238 683 56
BtsI GCAGTG_nn'
Cuts at: 3235 3493 4287 4307 3235
Size: 258 794 20 4905
Fragments arranged by size:
4905 794 258 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5977
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5942
DraI TTT'AAA
Cuts at: 2641 3281 2641
Size: 640 5337
DraIII CAC_nnn'GTG
Cuts at: 2149 2350 2149
Size: 201 5776
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4984 809
Size: 507 3668 1802
Fragments arranged by size:
3668 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3143 3424 4313
Size: 54 1425 50 275 18 1313 281 889
Cuts at: 4313 5942 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1313 889 281 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3424 1537
Size: 275 1612 4090
Fragments arranged by size:
4090 1612 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2538 2731 3907 1353
Size: 1185 193 1176 3423
Fragments arranged by size:
3423 1185 1176 193
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4564 4938 5084 387
Size: 1064 138 2975 374 146 1280
Fragments arranged by size:
2975 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2295 2318 2820 2865 2903 3095 4040
Size: 393 663 23 502 45 38 192 945
Cuts at: 4040 4637 5424 5692 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 945 787 663 597 502 393 268
192 45 38 23
EcoRI G'AATT_C
Cuts at: 3286 3664 3286
Size: 378 5599
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2574 2574
Size: 5977
FspI TGC'GCA
Cuts at: 4483 4483
Size: 5977
HaeII r_GCGC'y
Cuts at: 1678 5124 1678
Size: 3446 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2043 2075 2863 2895 4618
Size: 32 135 32 471 32 788 32 1723
Cuts at: 4618 4650 4692 4724 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1723 788 471 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3321 3567 669
Size: 988 281 1383 246 3079
Fragments arranged by size:
3079 1383 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2367 748
Size: 1619 4358
HpaI GTT'AAC
Cuts at: 3567 3567
Size: 5977
KpnI G_GTAC'C
Cuts at: 3302 3302
Size: 5977
MfeI C'AATT_G
Cuts at: 3576 3576
Size: 5977
MluI A'CGCG_T
Cuts at: 5853 5853
Size: 5977
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5091 5275 1305
Size: 3786 184 2007
Fragments arranged by size:
3786 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4155
Fragments arranged by size:
4155 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2174 3151 3162 3935 4294 4453 518
Size: 648 1008 977 11 773 359 159 2042
Fragments arranged by size:
2042 1008 977 773 648 359 159 11
MspA1I CmG'CkG
Cuts at: 1606 2687 3393 4070 5218 5463 1606
Size: 1081 706 677 1148 245 2120
Fragments arranged by size:
2120 1148 1081 706 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3217 513
Size: 552 939 1213 3273
Fragments arranged by size:
3273 1213 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5977
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5977
NotI GC'GGCC_GC
Cuts at: 3424 3424
Size: 5977
NsiI A_TGCA'T
Cuts at: 2973 2973
Size: 5977
NspI r_CATG'y
Cuts at: 1132 1229 2213 2743 3335 4880 1132
Size: 97 984 530 592 1545 2229
Fragments arranged by size:
2229 1545 984 592 530 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5977
PciI A'CATG_T
Cuts at: 2209 4876 2209
Size: 2667 3310
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5977
PmeI GTTT'AAAC
Cuts at: 3281 3281
Size: 5977
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4046 4078 5559 5591 4046
Size: 32 1481 32 4432
Fragments arranged by size:
4432 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2430 1613
Size: 817 5160
PsiI TTA'TAA
Cuts at: 3547 3547
Size: 5977
PspXI vC'TCGA_Gb
Cuts at: 2925 2925
Size: 5977
PstI C_TGCA'G
Cuts at: 830 3329 830
Size: 2499 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5977
PvuII CAG'CTG
Cuts at: 2687 2687
Size: 5977
SalI G'TCGA_C
Cuts at: 1936 3319 1936
Size: 1383 4594
SbfI CC_TGCA'GG
Cuts at: 3329 3329
Size: 5977
ScaI AGT'ACT
Cuts at: 4225 5937 4225
Size: 1712 4265
SfcI C'TryA_G
Cuts at: 826 1045 2568 2622 2778 3325 4460 5141 5332
Size: 219 1523 54 156 547 1135 681 191
Cuts at: 5332 826
Size: 1471
Fragments arranged by size:
1523 1471 1135 681 547 219 191 156
54
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5977
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5977
SmaI CCC'GGG
Cuts at: 1872 3304 1872
Size: 1432 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2925 4081 4982 5244 820
Size: 197 926 982 1156 901 262 1553
Fragments arranged by size:
1553 1156 982 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3768 493
Size: 3275 2702
SpeI A'CTAG_T
Cuts at: 152 3274 152
Size: 3122 2855
SphI G_CATG'C
Cuts at: 1132 3335 1132
Size: 2203 3774
SspI AAT'ATT
Cuts at: 5 52 3901 5
Size: 47 3849 2081
Fragments arranged by size:
3849 2081 47
StuI AGG'CCT
Cuts at: 1517 3250 1517
Size: 1733 4244
StyI C'CwwG_G
Cuts at: 513 1065 2004 3217 3416 513
Size: 552 939 1213 199 3074
Fragments arranged by size:
3074 1213 939 552 199
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4004 4021 4174 4359 4804 325
Size: 1255 2424 17 153 185 445 1498
Fragments arranged by size:
2424 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2681 3071 4223
Size: 274 80 33 51 1443 704 390 1152
Cuts at: 4223 5935 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1152 704 390 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5937 5969 5937
Size: 32 5945
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5977
XbaI T'CTAG_A
Cuts at: 3313 3313
Size: 5977
XhoI C'TCGA_G
Cuts at: 1943 2925 1943
Size: 982 4995
XmaI C'CCGG_G
Cuts at: 1870 3302 1870
Size: 1432 4545
XmnI GAAnn'nnTTC
Cuts at: 4106 4106
Size: 5977
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3785 276
Size: 53 83 186 936 2251 2468
Fragments arranged by size:
2468 2251 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PspXI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI