Restriction map for
pFN21AB9671
(Circular) (Six-base) MAPSORT of: pFN21AB9671.seq Check: 5296 from: 1 to: 6253
pFN21AB9671 6253 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4063 278
Size: 53 83 186 936 2527 2468
Fragments arranged by size:
2527 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3596 1937
Size: 1659 4594
Acc65I G'GTAC_C
Cuts at: 3574 3574
Size: 6253
AclI AA'CG_TT
Cuts at: 1563 4380 4753 1563
Size: 2817 373 3063
Fragments arranged by size:
3063 2817 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2124 2881 2965 3049 3217 4316 5700 5968
Size: 492 757 84 84 168 1099 1384 268
Cuts at: 5968 1632
Size: 1917
Fragments arranged by size:
1917 1384 1099 757 492 268 168 84
84
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6056
AflIII A'CryG_T
Cuts at: 1203 3545 5152 6129 1203
Size: 2342 1607 977 1327
Fragments arranged by size:
2342 1607 1327 977
AgeI A'CCGG_T
Cuts at: 5084 5084
Size: 6253
AhdI GACnn_n'nnGTC
Cuts at: 4982 4982
Size: 6253
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6253
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6253
AlwNI CAG_nnn'CTG
Cuts at: 3996 5568 3996
Size: 1572 4681
ApaLI G'TGCA_C
Cuts at: 4310 5466 4310
Size: 1156 5097
ApoI r'AATT_y
Cuts at: 1493 2427 2568 2595 2652 2931 3157 3241 3267
Size: 934 141 27 57 279 226 84 26
Cuts at: 3267 3351 3562 3788 3940 6049 1493
Size: 84 211 226 152 2109 1697
Fragments arranged by size:
2109 1697 934 279 226 226 211 152
141 84 84 57 27 26
AseI AT'TA_AT
Cuts at: 160 1032 4807 160
Size: 872 3775 1606
Fragments arranged by size:
3775 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6253
AvaI C'yCGr_G
Cuts at: 1870 1943 3578 1870
Size: 73 1635 4545
Fragments arranged by size:
4545 1635 73
BamHI G'GATC_C
Cuts at: 3583 3950 3583
Size: 367 5886
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3574 5029 618
Size: 325 217 627 1787 1455 1842
Fragments arranged by size:
1842 1787 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3572 1391
Size: 2181 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2467 2635 2803 2887 2971 3055 3223
Size: 971 568 168 168 84 84 84 168
Cuts at: 3223 3307 928
Size: 84 3874
Fragments arranged by size:
3874 971 568 168 168 168 84 84
84 84
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4444 4478 1903
Size: 34 50 34 2423 34 3678
Fragments arranged by size:
3678 2423 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4145 5355 4145
Size: 1210 5043
BclI T'GATC_A
Cuts at: 1548 2125 2760 1548
Size: 577 635 5041
Fragments arranged by size:
5041 635 577
BfrBI ATG'CAT
Cuts at: 2008 2008
Size: 6253
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4864 136
Size: 107 122 71 631 605 3192 1525
Fragments arranged by size:
3192 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2388 6248 2388
Size: 3860 2393
BlpI GC'TnA_GC
Cuts at: 3670 3670
Size: 6253
Bme1580I G_kGCm'C
Cuts at: 1790 4314 5470 1790
Size: 2524 1156 2573
Fragments arranged by size:
2573 2524 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6253
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3984 4942 442
Size: 416 53 83 399 24 2567 958 1753
Fragments arranged by size:
2567 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6253
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2457 2541 2709 2793 2877 2947 2961 3031
Size: 1218 84 168 84 84 70 14 70
Cuts at: 3031 3045 3213 3297 3381 3465 4913 1239
Size: 14 168 84 84 84 1448 2579
Fragments arranged by size:
2579 1448 1218 168 168 84 84 84
84 84 84 70 70 14 14
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4378 5243 5541 4378
Size: 865 298 5090
Fragments arranged by size:
5090 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4916 882
Size: 617 3417 2219
Fragments arranged by size:
3417 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4044 493
Size: 711 2840 2702
Fragments arranged by size:
2840 2702 711
BsaBI GATnn'nnATC
Cuts at: 6247 6247
Size: 6253
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4060 4442 5077 275
Size: 53 83 186 936 2527 382 635 1451
Fragments arranged by size:
2527 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2603 4686 5084 5358 5505 5916 2603
Size: 2083 398 274 147 411 2940
Fragments arranged by size:
2940 2083 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6028
Fragments arranged by size:
6028 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6253
BseYI C'CCAG_C
Cuts at: 5456 5952 5456
Size: 496 5757
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5680
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3703 4464 5492 1540
Size: 275 186 1702 761 1028 2301
Fragments arranged by size:
2301 1702 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4314 4399 5470 4314
Size: 85 1071 5097
Fragments arranged by size:
5097 1071 85
BsmI GAATG_Cn'
Cuts at: 3146 3764 3857 3146
Size: 618 93 5542
Fragments arranged by size:
5542 618 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2351 2351
Size: 6253
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3572 4314 4399 5470 1391
Size: 399 1782 742 85 1071 2174
Fragments arranged by size:
2174 1782 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5916 5916
Size: 6253
BspHI T'CATG_A
Cuts at: 4142 5872 4142
Size: 1730 4523
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3608 844
Size: 2764 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4140 1394
Size: 429 2317 3507
Fragments arranged by size:
3507 2317 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4748 4922 65
Size: 1183 3500 174 1396
Fragments arranged by size:
3500 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3107 4897 5084 1700
Size: 110 1297 1790 187 2869
Fragments arranged by size:
2869 1790 1297 187 110
BsrGI T'GTAC_A
Cuts at: 96 2749 3169 96
Size: 2653 420 3180
Fragments arranged by size:
3180 2653 420
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6253
BssSI C'ACGA_G
Cuts at: 4313 5325 4313
Size: 1012 5241
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6253
BstBI TT'CG_AA
Cuts at: 5057 5057
Size: 6253
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6253
BstYI r'GATC_y
Cuts at: 1972 2388 3583 3950 4335 4352 5804 5890 5902
Size: 416 1195 367 385 17 1452 86 12
Cuts at: 5902 6248 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1195 416 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5701
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2489 487
Size: 683 56 1263 4251
Fragments arranged by size:
4251 1263 683 56
BtsI GCAGTG_nn'
Cuts at: 3769 4563 4583 3769
Size: 794 20 5439
Fragments arranged by size:
5439 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6253
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6218
DraI TTT'AAA
Cuts at: 2368 3557 2368
Size: 1189 5064
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5260 809
Size: 507 3944 1802
Fragments arranged by size:
3944 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3700 4589 6218
Size: 54 1425 50 275 18 1870 889 1629
Cuts at: 6218 8
Size: 43
Fragments arranged by size:
1870 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3700 1537
Size: 275 1888 4090
Fragments arranged by size:
4090 1888 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3488 4183 1353
Size: 2135 695 3423
Fragments arranged by size:
3423 2135 695
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4840 5214 5360 387
Size: 1064 138 3251 374 146 1280
Fragments arranged by size:
3251 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2124 2457 2541 2709 2793 2877 2881
Size: 393 492 333 84 168 84 84 4
Cuts at: 2881 2947 2961 2965 3031 3045 3049 3213 3217
Size: 66 14 4 66 14 4 164 4
Cuts at: 3217 3297 3381 3465 4316 4913 5700 5968 1239
Size: 80 84 84 851 597 787 268 1524
Fragments arranged by size:
1524 851 787 597 492 393 333 268
168 164 84 84 84 84 84 80
66 66 14 14 4 4 4 4
EcoRI G'AATT_C
Cuts at: 2427 2595 2931 3267 3562 3940 2427
Size: 168 336 336 295 378 4740
Fragments arranged by size:
4740 378 336 336 295 168
FspI TGC'GCA
Cuts at: 4759 4759
Size: 6253
HaeII r_GCGC'y
Cuts at: 1678 5400 1678
Size: 3722 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4894 4926 4968 5000 1373
Size: 32 135 32 3322 32 42 32 2626
Fragments arranged by size:
3322 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2146 3597 3843 669
Size: 988 281 208 1451 246 3079
Fragments arranged by size:
3079 1451 988 281 246 208
HindIII A'AGCT_T
Cuts at: 748 3285 3396 748
Size: 2537 111 3605
Fragments arranged by size:
3605 2537 111
HpaI GTT'AAC
Cuts at: 3843 3843
Size: 6253
KpnI G_GTAC'C
Cuts at: 3578 3578
Size: 6253
MfeI C'AATT_G
Cuts at: 3852 3852
Size: 6253
MluI A'CGCG_T
Cuts at: 6129 6129
Size: 6253
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5367 5551 1305
Size: 4062 184 2007
Fragments arranged by size:
4062 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4431
Fragments arranged by size:
4431 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2214 2218 2452 2536 3108 4211 4570
Size: 648 1048 4 234 84 572 1103 359
Cuts at: 4570 4729 518
Size: 159 2042
Fragments arranged by size:
2042 1103 1048 648 572 359 234 159
84 4
MspA1I CmG'CkG
Cuts at: 1606 3669 4346 5494 5739 1606
Size: 2063 677 1148 245 2120
Fragments arranged by size:
2120 2063 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5701
NdeI CA'TA_TG
Cuts at: 387 2215 387
Size: 1828 4425
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6253
NotI GC'GGCC_GC
Cuts at: 3700 3700
Size: 6253
NsiI A_TGCA'T
Cuts at: 2010 2010
Size: 6253
NspI r_CATG'y
Cuts at: 1132 1229 3549 3611 5156 1132
Size: 97 2320 62 1545 2229
Fragments arranged by size:
2320 2229 1545 97 62
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6253
PciI A'CATG_T
Cuts at: 3545 5152 3545
Size: 1607 4646
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6253
PmeI GTTT'AAAC
Cuts at: 3557 3557
Size: 6253
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4322 4354 5835 5867 4322
Size: 32 1481 32 4708
Fragments arranged by size:
4708 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6253
PsiI TTA'TAA
Cuts at: 2619 3123 3291 3823 2619
Size: 504 168 532 5049
Fragments arranged by size:
5049 532 504 168
PstI C_TGCA'G
Cuts at: 830 2091 3605 830
Size: 1261 1514 3478
Fragments arranged by size:
3478 1514 1261
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6253
SalI G'TCGA_C
Cuts at: 1936 3595 1936
Size: 1659 4594
SbfI CC_TGCA'GG
Cuts at: 3605 3605
Size: 6253
ScaI AGT'ACT
Cuts at: 4501 6213 4501
Size: 1712 4541
SfcI C'TryA_G
Cuts at: 826 1045 2087 3601 4736 5417 5608 826
Size: 219 1042 1514 1135 681 191 1471
Fragments arranged by size:
1514 1471 1135 1042 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6253
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6253
SmaI CCC'GGG
Cuts at: 1872 3580 1872
Size: 1708 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4357 5258 5520 820
Size: 197 926 2414 901 262 1553
Fragments arranged by size:
2414 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4044 493
Size: 3551 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6253
SphI G_CATG'C
Cuts at: 1132 3611 1132
Size: 2479 3774
SspI AAT'ATT
Cuts at: 5 52 2266 4177 5
Size: 47 2214 1911 2081
Fragments arranged by size:
2214 2081 1911 47
StuI AGG'CCT
Cuts at: 1517 2306 1517
Size: 789 5464
StyI C'CwwG_G
Cuts at: 513 1065 3692 513
Size: 552 2627 3074
Fragments arranged by size:
3074 2627 552
SwaI ATTT'AAAT
Cuts at: 2368 2368
Size: 6253
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3308 4280 4297 4450 4635 5080 325
Size: 1255 1728 972 17 153 185 445 1498
Fragments arranged by size:
1728 1498 1255 972 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2749 3169 4499
Size: 274 80 33 51 1443 772 420 1330
Cuts at: 4499 6211 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1330 772 420 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2894 2926 2978 3010 6213 6245 2894
Size: 32 52 32 3203 32 2902
Fragments arranged by size:
3203 2902 52 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6253
XbaI T'CTAG_A
Cuts at: 3589 3589
Size: 6253
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6253
XmaI C'CCGG_G
Cuts at: 1870 3578 1870
Size: 1708 4545
XmnI GAAnn'nnTTC
Cuts at: 3314 3371 3455 4382 3314
Size: 57 84 927 5185
Fragments arranged by size:
5185 927 84 57
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4061 276
Size: 53 83 186 936 2527 2468
Fragments arranged by size:
2527 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI SwaI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI XcmI