Restriction map for
pFN21AB9668
(Circular) (Six-base) MAPSORT of: pFN21AB9668.seq Check: 1307 from: 1 to: 6199
pFN21AB9668 6199 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2023 2023
Size: 6199
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4009 278
Size: 53 83 186 936 2473 2468
Fragments arranged by size:
2473 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3542 1937
Size: 1605 4594
Acc65I G'GTAC_C
Cuts at: 3520 3520
Size: 6199
AclI AA'CG_TT
Cuts at: 1563 4326 4699 1563
Size: 2763 373 3063
Fragments arranged by size:
3063 2763 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2585 2668 2752 2819 3088 4262 5646 5914
Size: 953 83 84 67 269 1174 1384 268
Cuts at: 5914 1632
Size: 1917
Fragments arranged by size:
1917 1384 1174 953 269 268 84 83
67
AfeI AGC'GCT
Cuts at: 3113 3113
Size: 6199
AflII C'TTAA_G
Cuts at: 820 1017 3376 820
Size: 197 2359 3643
Fragments arranged by size:
3643 2359 197
AflIII A'CryG_T
Cuts at: 1203 2406 5098 6075 1203
Size: 1203 2692 977 1327
Fragments arranged by size:
2692 1327 1203 977
AgeI A'CCGG_T
Cuts at: 5030 5030
Size: 6199
AhdI GACnn_n'nnGTC
Cuts at: 4928 4928
Size: 6199
AleI CACnn'nnGTG
Cuts at: 1166 2618 2979 1166
Size: 1452 361 4386
Fragments arranged by size:
4386 1452 361
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6199
AlwNI CAG_nnn'CTG
Cuts at: 2956 3307 3942 5514 2956
Size: 351 635 1572 3641
Fragments arranged by size:
3641 1572 635 351
ApaI G_GGCC'C
Cuts at: 2231 2262 2231
Size: 31 6168
ApaLI G'TGCA_C
Cuts at: 2384 2720 3056 4256 5412 2384
Size: 336 336 1200 1156 3171
Fragments arranged by size:
3171 1200 1156 336 336
ApoI r'AATT_y
Cuts at: 1493 3508 3734 3886 5995 1493
Size: 2015 226 152 2109 1697
Fragments arranged by size:
2109 2015 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4753 160
Size: 872 3721 1606
Fragments arranged by size:
3721 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6199
AvaI C'yCGr_G
Cuts at: 1870 1943 2414 3145 3524 1870
Size: 73 471 731 379 4545
Fragments arranged by size:
4545 731 471 379 73
BamHI G'GATC_C
Cuts at: 3529 3896 3529
Size: 367 5832
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3149 3520 4975 618
Size: 325 217 627 1362 371 1455 1842
Fragments arranged by size:
1842 1455 1362 627 371 325 217
BanII G_rGCy'C
Cuts at: 1391 2231 2262 2360 2444 2780 2948 3032 3375
Size: 840 31 98 84 336 168 84 343
Cuts at: 3375 3518 1391
Size: 143 4072
Fragments arranged by size:
4072 840 343 336 168 143 98 84
84 31
BbeI G_GCGC'C
Cuts at: 3153 3153
Size: 6199
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2078 928
Size: 971 179 5049
Fragments arranged by size:
5049 971 179
BbvCI CC'TCA_GC
Cuts at: 2450 2450
Size: 6199
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2413 2447 2581 2615 2833 2867 3337
Size: 34 476 34 134 34 218 34 470
Cuts at: 3337 3371 4390 4424 1903
Size: 34 1019 34 3678
Fragments arranged by size:
3678 1019 476 470 218 134 34 34
34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4091 5301 4091
Size: 1210 4989
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6199
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4810 136
Size: 107 122 71 631 605 3138 1525
Fragments arranged by size:
3138 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6194 6194
Size: 6199
BlpI GC'TnA_GC
Cuts at: 3616 3616
Size: 6199
Bme1580I G_kGCm'C
Cuts at: 1790 2231 2262 2388 2414 2666 2724 3060 4260
Size: 441 31 126 26 252 58 336 1200
Cuts at: 4260 5416 1790
Size: 1156 2573
Fragments arranged by size:
2573 1200 1156 441 336 252 126 58
31 26
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6199
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2195 3082 3930
Size: 416 53 83 399 24 778 887 848
Cuts at: 3930 4888 442
Size: 958 1753
Fragments arranged by size:
1753 958 887 848 778 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6199
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2130 2458 2726 4859 1239
Size: 891 328 268 2133 2579
Fragments arranged by size:
2579 2133 891 328 268
Bpu10I CC'TnA_GC
Cuts at: 2450 2450
Size: 6199
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4324 5189 5487 4324
Size: 865 298 5036
Fragments arranged by size:
5036 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4862 882
Size: 617 3363 2219
Fragments arranged by size:
3363 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2409 3990 493
Size: 711 1205 1581 2702
Fragments arranged by size:
2702 1581 1205 711
BsaBI GATnn'nnATC
Cuts at: 6193 6193
Size: 6199
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3150 4006 4388 5023
Size: 53 83 186 936 1617 856 382 635
Cuts at: 5023 275
Size: 1451
Fragments arranged by size:
1617 1451 936 856 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4632 5030 5304 5451 5862 4632
Size: 398 274 147 411 4969
Fragments arranged by size:
4969 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5974
Fragments arranged by size:
5974 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2324 1186
Size: 1138 5061
BseYI C'CCAG_C
Cuts at: 2115 2224 2255 5402 5898 2115
Size: 109 31 3147 496 2416
Fragments arranged by size:
3147 2416 496 109 31
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2642 3293 1163
Size: 573 906 651 4069
Fragments arranged by size:
4069 906 651 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2605 2738 3649 4410 5438 1540
Size: 275 186 604 133 911 761 1028 2301
Fragments arranged by size:
2301 1028 911 761 604 275 186 133
BsiHKAI G_wGCw'C
Cuts at: 2388 2444 2712 2724 2780 2796 2948 3048 3060
Size: 56 268 12 56 16 152 100 12
Cuts at: 3060 3375 3468 4260 4345 5416 2388
Size: 315 93 792 85 1071 3171
Fragments arranged by size:
3171 1071 792 315 268 152 100 93
85 56 56 16 12 12
BsmI GAATG_Cn'
Cuts at: 3263 3710 3803 3263
Size: 447 93 5659
Fragments arranged by size:
5659 447 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2231 2262 2360 2388 2414 2444 2666
Size: 399 441 31 98 28 26 30 222
Cuts at: 2666 2712 2724 2780 2796 2948 3032 3048 3060
Size: 46 12 56 16 152 84 16 12
Cuts at: 3060 3375 3468 3518 4260 4345 5416 1391
Size: 315 93 50 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 441 399 315 222 152
98 93 85 84 56 50 46 31
30 28 26 16 16 12 12
BspEI T'CCGG_A
Cuts at: 5862 5862
Size: 6199
BspHI T'CATG_A
Cuts at: 4088 5818 4088
Size: 1730 4469
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2023 3554 844
Size: 1179 1531 3489
Fragments arranged by size:
3489 1531 1179
BsrBI CCG'CTC
Cuts at: 1394 1823 2986 4086 1394
Size: 429 1163 1100 3507
Fragments arranged by size:
3507 1163 1100 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2071 4694 4868 65
Size: 1183 823 2623 174 1396
Fragments arranged by size:
2623 1396 1183 823 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2322 2712 2880 3048 3132 3216 3384
Size: 110 512 390 168 168 84 84 168
Cuts at: 3384 4843 5030 1700
Size: 1459 187 2869
Fragments arranged by size:
2869 1459 512 390 187 168 168 168
110 84 84
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6199
BssHII G'CGCG_C
Cuts at: 1925 2520 2730 1925
Size: 595 210 5394
Fragments arranged by size:
5394 595 210
BssSI C'ACGA_G
Cuts at: 2080 4259 5271 2080
Size: 2179 1012 3008
Fragments arranged by size:
3008 2179 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2502 2956 794
Size: 1708 454 4037
Fragments arranged by size:
4037 1708 454
BstBI TT'CG_AA
Cuts at: 5003 5003
Size: 6199
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6199
BstXI CCAn_nnnn'nTGG
Cuts at: 2022 2704 2022
Size: 682 5517
BstYI r'GATC_y
Cuts at: 1972 2802 3529 3896 4281 4298 5750 5836 5848
Size: 830 727 367 385 17 1452 86 12
Cuts at: 5848 6194 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 830 727 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2136 3190 3274 513
Size: 552 939 132 1054 84 3438
Fragments arranged by size:
3438 1054 939 552 132 84
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5460
Fragments arranged by size:
5460 683 56
BtsI GCAGTG_nn'
Cuts at: 3340 3715 4509 4529 3340
Size: 375 794 20 5010
Fragments arranged by size:
5010 794 375 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6199
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6164
DraI TTT'AAA
Cuts at: 3503 3503
Size: 6199
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5206 809
Size: 507 3890 1802
Fragments arranged by size:
3890 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2164 2602 2735
Size: 54 1425 50 275 18 334 438 133
Cuts at: 2735 2787 2819 3010 3646 4535 6164 8
Size: 52 32 191 636 889 1629 43
Fragments arranged by size:
1629 1425 889 636 438 334 275 191
133 54 52 50 43 32 18
EagI C'GGCC_G
Cuts at: 1537 1812 2602 2735 3646 1537
Size: 275 790 133 911 4090
Fragments arranged by size:
4090 911 790 275 133
EarI CTCTTCn'nnn_
Cuts at: 1353 4129 1353
Size: 2776 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2210 3056 4786 5160 5306 387
Size: 1064 138 621 846 1730 374 146 1280
Fragments arranged by size:
1730 1280 1064 846 621 374 146 138
EcoICRI GAG'CTC
Cuts at: 2442 2778 2946 3373 2442
Size: 336 168 427 5268
Fragments arranged by size:
5268 427 336 168
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2130 2458 2585 2668 2726 2752 2819
Size: 393 498 328 127 83 58 26 67
Cuts at: 2819 3088 4262 4859 5646 5914 1239
Size: 269 1174 597 787 268 1524
Fragments arranged by size:
1524 1174 787 597 498 393 328 269
268 127 83 67 58 26
EcoNI CCTnn'n_nnAGG
Cuts at: 2093 2093
Size: 6199
EcoO109I rG'GnC_Cy
Cuts at: 2056 2086 2121 2210 2228 3408 2056
Size: 30 35 89 18 1180 4847
Fragments arranged by size:
4847 1180 89 35 30 18
EcoRI G'AATT_C
Cuts at: 3508 3886 3508
Size: 378 5821
FspI TGC'GCA
Cuts at: 3253 3362 4705 3253
Size: 109 1343 4747
Fragments arranged by size:
4747 1343 109
HaeII r_GCGC'y
Cuts at: 1678 3115 3153 3307 5346 1678
Size: 1437 38 154 2039 2531
Fragments arranged by size:
2531 2039 1437 154 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4840 4872 4914 4946 1373
Size: 32 135 32 3268 32 42 32 2626
Fragments arranged by size:
3268 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3543 3789 669
Size: 988 281 1605 246 3079
Fragments arranged by size:
3079 1605 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6199
HpaI GTT'AAC
Cuts at: 3789 3789
Size: 6199
KasI G'GCGC_C
Cuts at: 3149 3149
Size: 6199
KpnI G_GTAC'C
Cuts at: 3524 3524
Size: 6199
MfeI C'AATT_G
Cuts at: 3798 3798
Size: 6199
MluI A'CGCG_T
Cuts at: 6075 6075
Size: 6199
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2469 2805 3141 3348 5313 5497 1305
Size: 1164 336 336 207 1965 184 2007
Fragments arranged by size:
2007 1965 1164 336 336 207 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4377
Fragments arranged by size:
4377 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2618 2979 4157 4516 4675 518
Size: 648 1452 361 1178 359 159 2042
Fragments arranged by size:
2042 1452 1178 648 361 359 159
MspA1I CmG'CkG
Cuts at: 1606 2170 2475 2559 2577 2688 3192 3276 3615
Size: 564 305 84 18 111 504 84 339
Cuts at: 3615 4292 5440 5685 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 564 504 339 305 245
111 84 84 18
NarI GG'CG_CC
Cuts at: 3150 3150
Size: 6199
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4708
Fragments arranged by size:
4708 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6199
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6199
NotI GC'GGCC_GC
Cuts at: 2735 3646 2735
Size: 911 5288
NspI r_CATG'y
Cuts at: 1132 1229 3557 5102 1132
Size: 97 2328 1545 2229
Fragments arranged by size:
2328 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6199
PciI A'CATG_T
Cuts at: 5098 5098
Size: 6199
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6199
PfoI T'CCnGG_A
Cuts at: 2703 2703
Size: 6199
PmeI GTTT'AAAC
Cuts at: 3503 3503
Size: 6199
PmlI CAC'GTG
Cuts at: 2409 2409
Size: 6199
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3311 3343 4268 4300 5781 5813 3311
Size: 32 925 32 1481 32 3697
Fragments arranged by size:
3697 1481 925 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2056 2056
Size: 6199
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6199
PsiI TTA'TAA
Cuts at: 3769 3769
Size: 6199
PspOMI G'GGCC_C
Cuts at: 2227 2258 2227
Size: 31 6168
PstI C_TGCA'G
Cuts at: 830 2034 2278 2373 2961 3347 3551 830
Size: 1204 244 95 588 386 204 3478
Fragments arranged by size:
3478 1204 588 386 244 204 95
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6199
PvuII CAG'CTG
Cuts at: 2688 2688
Size: 6199
SacI G_AGCT'C
Cuts at: 2444 2780 2948 3375 2444
Size: 336 168 427 5268
Fragments arranged by size:
5268 427 336 168
SacII CC_GC'GG
Cuts at: 3193 3277 3193
Size: 84 6115
SalI G'TCGA_C
Cuts at: 1936 3541 1936
Size: 1605 4594
SbfI CC_TGCA'GG
Cuts at: 2034 3551 2034
Size: 1517 4682
ScaI AGT'ACT
Cuts at: 4447 6159 4447
Size: 1712 4487
SfcI C'TryA_G
Cuts at: 826 1045 2030 2274 2369 2957 3343 3547 4682
Size: 219 985 244 95 588 386 204 1135
Cuts at: 4682 5363 5554 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 985 681 588 386 244 219
204 191 95
SfoI GGC'GCC
Cuts at: 3151 3151
Size: 6199
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6199
SgrAI Cr'CCGG_yG
Cuts at: 1700 2712 2880 3048 3132 3384 1700
Size: 1012 168 168 84 252 4515
Fragments arranged by size:
4515 1012 252 168 168 84
SmaI CCC'GGG
Cuts at: 1872 3526 1872
Size: 1654 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3376 4303 5204 5466 820
Size: 197 926 1433 927 901 262 1553
Fragments arranged by size:
1553 1433 927 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3990 493
Size: 3497 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6199
SphI G_CATG'C
Cuts at: 1132 3557 1132
Size: 2425 3774
SspI AAT'ATT
Cuts at: 5 52 4123 5
Size: 47 4071 2081
Fragments arranged by size:
4071 2081 47
StuI AGG'CCT
Cuts at: 1517 2429 2513 2681 2765 2849 3101 3185 3269
Size: 912 84 168 84 84 252 84 84
Cuts at: 3269 3353 1517
Size: 84 4363
Fragments arranged by size:
4363 912 252 168 84 84 84 84
84 84
StyI C'CwwG_G
Cuts at: 513 1065 2004 3638 513
Size: 552 939 1634 3074
Fragments arranged by size:
3074 1634 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4226 4243 4396 4581 5026 325
Size: 1255 2646 17 153 185 445 1498
Fragments arranged by size:
2646 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4445 6157 96
Size: 274 80 33 51 1443 2468 1712 138
Fragments arranged by size:
2468 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2128 2160 6159 6191 2128
Size: 32 3999 32 2136
Fragments arranged by size:
3999 2136 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6199
XbaI T'CTAG_A
Cuts at: 3535 3535
Size: 6199
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6199
XmaI C'CCGG_G
Cuts at: 1870 3524 1870
Size: 1654 4545
XmnI GAAnn'nnTTC
Cuts at: 2570 2906 3437 4328 2570
Size: 336 531 891 4441
Fragments arranged by size:
4441 891 531 336
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4007 276
Size: 53 83 186 936 2473 2468
Fragments arranged by size:
2473 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SacII SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI
BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI NaeI
NgoMIV NruI NsiI PacI PspXI PsrI RsrII SanDI
SapI SexAI SfiI SrfI SwaI XcmI