Restriction map for
pFN21AB9667
(Circular) (Six-base) MAPSORT of: pFN21AB9667.seq Check: 2453 from: 1 to: 5635
pFN21AB9667 5635 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3445 278
Size: 53 83 186 936 1909 2468
Fragments arranged by size:
2468 1909 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2978 1937
Size: 1041 4594
Acc65I G'GTAC_C
Cuts at: 2956 2956
Size: 5635
AclI AA'CG_TT
Cuts at: 1563 2824 3762 4135 1563
Size: 1261 938 373 3063
Fragments arranged by size:
3063 1261 938 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2039 3698 5082 5350 1632
Size: 407 1659 1384 268 1917
Fragments arranged by size:
1917 1659 1384 407 268
AfeI AGC'GCT
Cuts at: 2768 2768
Size: 5635
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5438
AflIII A'CryG_T
Cuts at: 1203 4534 5511 1203
Size: 3331 977 1327
Fragments arranged by size:
3331 1327 977
AgeI A'CCGG_T
Cuts at: 4466 4466
Size: 5635
AhdI GACnn_n'nnGTC
Cuts at: 4364 4364
Size: 5635
AleI CACnn'nnGTG
Cuts at: 1166 2658 1166
Size: 1492 4143
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5635
AlwNI CAG_nnn'CTG
Cuts at: 2017 3378 4950 2017
Size: 1361 1572 2702
Fragments arranged by size:
2702 1572 1361
ApaLI G'TGCA_C
Cuts at: 2743 3692 4848 2743
Size: 949 1156 3530
Fragments arranged by size:
3530 1156 949
ApoI r'AATT_y
Cuts at: 1493 2419 2513 2944 3170 3322 5431 1493
Size: 926 94 431 226 152 2109 1697
Fragments arranged by size:
2109 1697 926 431 226 152 94
AseI AT'TA_AT
Cuts at: 160 1032 4189 160
Size: 872 3157 1606
Fragments arranged by size:
3157 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5635
AvaI C'yCGr_G
Cuts at: 1870 1943 2646 2960 1870
Size: 73 703 314 4545
Fragments arranged by size:
4545 703 314 73
BamHI G'GATC_C
Cuts at: 2965 3332 2965
Size: 367 5268
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2956 4411 618
Size: 325 217 627 1169 1455 1842
Fragments arranged by size:
1842 1455 1169 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2068 2693 2954 1391
Size: 677 625 261 4072
Fragments arranged by size:
4072 677 625 261
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4664
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3826 3860 1903
Size: 34 1889 34 3678
Fragments arranged by size:
3678 1889 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3527 4737 3527
Size: 1210 4425
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5635
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4246 136
Size: 107 122 71 631 605 2574 1525
Fragments arranged by size:
2574 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5630 5630
Size: 5635
BlpI GC'TnA_GC
Cuts at: 2079 3052 2079
Size: 973 4662
Bme1580I G_kGCm'C
Cuts at: 1790 2747 3696 4852 1790
Size: 957 949 1156 2573
Fragments arranged by size:
2573 1156 957 949
BmgBI CAC'GTC
Cuts at: 1318 2656 1318
Size: 1338 4297
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2064 3366 4324
Size: 416 53 83 399 24 647 1302 958
Cuts at: 4324 442
Size: 1753
Fragments arranged by size:
1753 1302 958 647 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5635
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2487 2745 4295 1239
Size: 1248 258 1550 2579
Fragments arranged by size:
2579 1550 1248 258
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2447 3760 4625 4923 2447
Size: 1313 865 298 3159
Fragments arranged by size:
3159 1313 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2643 4298 882
Size: 617 1144 1655 2219
Fragments arranged by size:
2219 1655 1144 617
BsaAI yAC'GTr
Cuts at: 493 1204 2800 3426 493
Size: 711 1596 626 2702
Fragments arranged by size:
2702 1596 711 626
BsaBI GATnn'nnATC
Cuts at: 5629 5629
Size: 5635
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3442 3824 4459 275
Size: 53 83 186 936 1909 382 635 1451
Fragments arranged by size:
1909 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4068 4466 4740 4887 5298 4068
Size: 398 274 147 411 4405
Fragments arranged by size:
4405 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5410
Fragments arranged by size:
5410 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2621 1186
Size: 1435 4200
BseYI C'CCAG_C
Cuts at: 4838 5334 4838
Size: 496 5139
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5062
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3085 3846 4874 1540
Size: 275 186 1084 761 1028 2301
Fragments arranged by size:
2301 1084 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2693 2747 3696 3781 4852 2693
Size: 54 949 85 1071 3476
Fragments arranged by size:
3476 1071 949 85 54
BsiWI C'GTAC_G
Cuts at: 2792 2792
Size: 5635
BsmI GAATG_Cn'
Cuts at: 2231 3146 3239 2231
Size: 915 93 4627
Fragments arranged by size:
4627 915 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2068 2693 2747 2954 3696 3781 4852
Size: 399 278 625 54 207 742 85 1071
Cuts at: 4852 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 625 399 278 207 85
54
BspEI T'CCGG_A
Cuts at: 5298 5298
Size: 5635
BspHI T'CATG_A
Cuts at: 3524 5254 3524
Size: 1730 3905
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2118 2990 844
Size: 1274 872 3489
Fragments arranged by size:
3489 1274 872
BsrBI CCG'CTC
Cuts at: 1394 1823 3522 1394
Size: 429 1699 3507
Fragments arranged by size:
3507 1699 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4130 4304 65
Size: 1183 2882 174 1396
Fragments arranged by size:
2882 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4279 4466 1700
Size: 110 2469 187 2869
Fragments arranged by size:
2869 2469 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5635
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5635
BssSI C'ACGA_G
Cuts at: 3695 4707 3695
Size: 1012 4623
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2516 794
Size: 1722 3913
BstBI TT'CG_AA
Cuts at: 2337 4439 2337
Size: 2102 3533
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5635
BstYI r'GATC_y
Cuts at: 1972 2494 2965 3332 3717 3734 5186 5272 5284
Size: 522 471 367 385 17 1452 86 12
Cuts at: 5284 5630 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 522 471 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2184 2184
Size: 5635
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5083
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4896
Fragments arranged by size:
4896 683 56
BtsI GCAGTG_nn'
Cuts at: 3151 3945 3965 3151
Size: 794 20 4821
Fragments arranged by size:
4821 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5635
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5600
DraI TTT'AAA
Cuts at: 2939 2939
Size: 5635
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4642 809
Size: 507 3326 1802
Fragments arranged by size:
3326 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3082 3971 5600
Size: 54 1425 50 275 18 1252 889 1629
Cuts at: 5600 8
Size: 43
Fragments arranged by size:
1629 1425 1252 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3082 1537
Size: 275 1270 4090
Fragments arranged by size:
4090 1270 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2056 2259 3565 1353
Size: 703 203 1306 3423
Fragments arranged by size:
3423 1306 703 203
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2132 2451 4222 4596 4742 387
Size: 1064 138 543 319 1771 374 146 1280
Fragments arranged by size:
1771 1280 1064 543 374 319 146 138
EcoICRI GAG'CTC
Cuts at: 2691 2691
Size: 5635
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2039 2487 2745 3698 4295 5082 5350
Size: 393 407 448 258 953 597 787 268
Cuts at: 5350 1239
Size: 1524
Fragments arranged by size:
1524 953 787 597 448 407 393 268
258
EcoNI CCTnn'n_nnAGG
Cuts at: 2264 2264
Size: 5635
EcoRI G'AATT_C
Cuts at: 2419 2944 3322 2419
Size: 525 378 4732
Fragments arranged by size:
4732 525 378
FspI TGC'GCA
Cuts at: 2804 4141 2804
Size: 1337 4298
FspAI rTGC'GCAy
Cuts at: 2804 2804
Size: 5635
HaeII r_GCGC'y
Cuts at: 1678 2770 4782 1678
Size: 1092 2012 2531
Fragments arranged by size:
2531 2012 1092
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2192 2224 2611 2640 2643
Size: 32 135 32 620 32 387 29 3
Cuts at: 2643 2672 4276 4308 4350 4382 1373
Size: 29 1604 32 42 32 2626
Fragments arranged by size:
2626 1604 620 387 135 42 32 32
32 32 32 29 29 3
HincII GTy'rAC
Cuts at: 669 1657 1938 2979 3225 669
Size: 988 281 1041 246 3079
Fragments arranged by size:
3079 1041 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2456 748
Size: 1708 3927
HpaI GTT'AAC
Cuts at: 3225 3225
Size: 5635
KpnI G_GTAC'C
Cuts at: 2960 2960
Size: 5635
MfeI C'AATT_G
Cuts at: 3234 3234
Size: 5635
MluI A'CGCG_T
Cuts at: 5511 5511
Size: 5635
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2392 4749 4933 1305
Size: 1087 2357 184 2007
Fragments arranged by size:
2357 2007 1087 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3813
Fragments arranged by size:
3813 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2658 3593 3952 4111 518
Size: 648 1492 935 359 159 2042
Fragments arranged by size:
2042 1492 935 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3051 3728 4876 5121 1606
Size: 1445 677 1148 245 2120
Fragments arranged by size:
2120 1445 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5083
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5635
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5635
NotI GC'GGCC_GC
Cuts at: 3082 3082
Size: 5635
NspI r_CATG'y
Cuts at: 1132 1229 2903 2993 4538 1132
Size: 97 1674 90 1545 2229
Fragments arranged by size:
2229 1674 1545 97 90
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5635
PciI A'CATG_T
Cuts at: 4534 4534
Size: 5635
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5635
PfoI T'CCnGG_A
Cuts at: 2259 2259
Size: 5635
PmeI GTTT'AAAC
Cuts at: 2939 2939
Size: 5635
PmlI CAC'GTG
Cuts at: 2800 2800
Size: 5635
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3704 3736 5217 5249 3704
Size: 32 1481 32 4090
Fragments arranged by size:
4090 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5635
PsiI TTA'TAA
Cuts at: 3205 3205
Size: 5635
PstI C_TGCA'G
Cuts at: 830 2115 2987 830
Size: 1285 872 3478
Fragments arranged by size:
3478 1285 872
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5635
SacI G_AGCT'C
Cuts at: 2693 2693
Size: 5635
SalI G'TCGA_C
Cuts at: 1936 2977 1936
Size: 1041 4594
SapI GCTCTTCn'nnn_
Cuts at: 2056 2056
Size: 5635
SbfI CC_TGCA'GG
Cuts at: 2115 2987 2115
Size: 872 4763
ScaI AGT'ACT
Cuts at: 3883 5595 3883
Size: 1712 3923
SfcI C'TryA_G
Cuts at: 826 1045 2111 2983 4118 4799 4990 826
Size: 219 1066 872 1135 681 191 1471
Fragments arranged by size:
1471 1135 1066 872 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5635
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5635
SmaI CCC'GGG
Cuts at: 1872 2962 1872
Size: 1090 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2426 3739 4640 4902 820
Size: 197 926 483 1313 901 262 1553
Fragments arranged by size:
1553 1313 926 901 483 262 197
SnaBI TAC'GTA
Cuts at: 493 3426 493
Size: 2933 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5635
SphI G_CATG'C
Cuts at: 1132 2903 2993 1132
Size: 1771 90 3774
Fragments arranged by size:
3774 1771 90
SspI AAT'ATT
Cuts at: 5 52 3559 5
Size: 47 3507 2081
Fragments arranged by size:
3507 2081 47
StuI AGG'CCT
Cuts at: 1517 2052 1517
Size: 535 5100
StyI C'CwwG_G
Cuts at: 513 1065 2286 3074 513
Size: 552 1221 788 3074
Fragments arranged by size:
3074 1221 788 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2045 3662 3679 3832 4017 4462 325
Size: 1255 465 1617 17 153 185 445 1498
Fragments arranged by size:
1617 1498 1255 465 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2439 3881 5593
Size: 274 80 33 51 1443 462 1442 1712
Cuts at: 5593 96
Size: 138
Fragments arranged by size:
1712 1443 1442 462 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5595 5627 5595
Size: 32 5603
Tth111I GACn'n_nGTC
Cuts at: 1316 2654 1316
Size: 1338 4297
XbaI T'CTAG_A
Cuts at: 2971 2971
Size: 5635
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5635
XmaI C'CCGG_G
Cuts at: 1870 2960 1870
Size: 1090 4545
XmnI GAAnn'nnTTC
Cuts at: 2678 2825 3764 2678
Size: 147 939 4549
Fragments arranged by size:
4549 939 147
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3443 276
Size: 53 83 186 936 1909 2468
Fragments arranged by size:
2468 1909 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoRI FspI FspAI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PstI PvuI SacI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsmBI BstXI BstZ17I DraIII EcoO109I
EcoRV FalI FseI KasI NaeI NarI NgoMIV NruI
NsiI PacI PpuMI PspOMI PspXI PsrI PvuII RsrII
SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI