Restriction map for
pFN21AB9666
(Circular) (Six-base) MAPSORT of: pFN21AB9666.seq Check: 8076 from: 1 to: 6148
pFN21AB9666 6148 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2511 2595 2763 2847 2511
Size: 84 168 84 5812
Fragments arranged by size:
5812 168 84 84
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3958 278
Size: 53 83 186 936 2422 2468
Fragments arranged by size:
2468 2422 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3491 1937
Size: 1554 4594
Acc65I G'GTAC_C
Cuts at: 3469 3469
Size: 6148
AclI AA'CG_TT
Cuts at: 1563 4275 4648 1563
Size: 2712 373 3063
Fragments arranged by size:
3063 2712 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2290 4211 5595 5863 1632
Size: 658 1921 1384 268 1917
Fragments arranged by size:
1921 1917 1384 658 268
AfeI AGC'GCT
Cuts at: 2826 2826
Size: 6148
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5951
AflIII A'CryG_T
Cuts at: 1203 5047 6024 1203
Size: 3844 977 1327
Fragments arranged by size:
3844 1327 977
AgeI A'CCGG_T
Cuts at: 4979 4979
Size: 6148
AhdI GACnn_n'nnGTC
Cuts at: 4877 4877
Size: 6148
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6148
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2955 1184
Size: 1771 4377
AlwNI CAG_nnn'CTG
Cuts at: 2503 2839 2948 3302 3891 5463 2503
Size: 336 109 354 589 1572 3188
Fragments arranged by size:
3188 1572 589 354 336 109
ApaLI G'TGCA_C
Cuts at: 4205 5361 4205
Size: 1156 4992
ApoI r'AATT_y
Cuts at: 1493 3457 3683 3835 5944 1493
Size: 1964 226 152 2109 1697
Fragments arranged by size:
2109 1964 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4702 160
Size: 872 3670 1606
Fragments arranged by size:
3670 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6148
AvaI C'yCGr_G
Cuts at: 1870 1943 3002 3473 1870
Size: 73 1059 471 4545
Fragments arranged by size:
4545 1059 471 73
BamHI G'GATC_C
Cuts at: 3160 3478 3845 3160
Size: 318 367 5463
Fragments arranged by size:
5463 367 318
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2315 3107 3469 4924 618
Size: 325 217 627 528 792 362 1455 1842
Fragments arranged by size:
1842 1455 792 627 528 362 325 217
BanII G_rGCy'C
Cuts at: 1391 2570 2654 2738 2822 3467 1391
Size: 1179 84 84 84 645 4072
Fragments arranged by size:
4072 1179 645 84 84 84
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2001 928
Size: 971 102 5075
Fragments arranged by size:
5075 971 102
BbvCI CC'TCA_GC
Cuts at: 2081 3098 2081
Size: 1017 5131
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2371 2405 2455 2489 2623 2657 2707
Size: 34 434 34 50 34 134 34 50
Cuts at: 2707 2741 4339 4373 1903
Size: 34 1598 34 3678
Fragments arranged by size:
3678 1598 434 134 50 50 34 34
34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4040 5250 4040
Size: 1210 4938
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6148
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2324 4759 136
Size: 107 122 71 631 605 652 2435 1525
Fragments arranged by size:
2435 1525 652 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6143 6143
Size: 6148
BlpI GC'TnA_GC
Cuts at: 2240 2328 2821 3565 2240
Size: 88 493 744 4823
Fragments arranged by size:
4823 744 493 88
Bme1580I G_kGCm'C
Cuts at: 1790 2481 2540 3152 3243 3267 3291 3315 3430
Size: 691 59 612 91 24 24 24 115
Cuts at: 3430 4209 5365 1790
Size: 779 1156 2573
Fragments arranged by size:
2573 1156 779 691 612 115 91 59
24 24 24
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6148
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2358 3879 4837
Size: 416 53 83 399 24 941 1521 958
Cuts at: 4837 442
Size: 1753
Fragments arranged by size:
1753 1521 958 941 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6148
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2816 2890 3172 4808 1239
Size: 1577 74 282 1636 2579
Fragments arranged by size:
2579 1636 1577 282 74
Bpu10I CC'TnA_GC
Cuts at: 2081 3014 3098 2081
Size: 933 84 5131
Fragments arranged by size:
5131 933 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4273 5138 5436 4273
Size: 865 298 4985
Fragments arranged by size:
4985 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3052 4811 882
Size: 617 1553 1759 2219
Fragments arranged by size:
2219 1759 1553 617
BsaAI yAC'GTr
Cuts at: 493 1204 2845 3939 493
Size: 711 1641 1094 2702
Fragments arranged by size:
2702 1641 1094 711
BsaBI GATnn'nnATC
Cuts at: 6142 6142
Size: 6148
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3955 4337 4972 275
Size: 53 83 186 936 2422 382 635 1451
Fragments arranged by size:
2422 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4581 4979 5253 5400 5811 4581
Size: 398 274 147 411 4918
Fragments arranged by size:
4918 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2096 2126 1178
Size: 30 165 30 693 30 5200
Fragments arranged by size:
5200 693 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2104 1186
Size: 918 5230
BseYI C'CCAG_C
Cuts at: 3164 3200 3236 3266 3290 3314 3379 3433 5351
Size: 36 36 30 24 24 65 54 1918
Cuts at: 5351 5847 3164
Size: 496 3465
Fragments arranged by size:
3465 1918 496 65 54 36 36 30
24 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2825 2961 1163
Size: 573 1089 136 4350
Fragments arranged by size:
4350 1089 573 136
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2360 2688 2967 2988 3598 4359
Size: 275 186 359 328 279 21 610 761
Cuts at: 4359 5387 1540
Size: 1028 2301
Fragments arranged by size:
2301 1028 761 610 359 328 279 275
186 21
BsiHKAI G_wGCw'C
Cuts at: 2166 2654 2738 2822 3081 4209 4294 5365 2166
Size: 488 84 84 259 1128 85 1071 2949
Fragments arranged by size:
2949 1128 1071 488 259 85 84 84
BsmI GAATG_Cn'
Cuts at: 2848 3659 3752 2848
Size: 811 93 5244
Fragments arranged by size:
5244 811 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2166 2481 2540 2570 2654 2738 2822
Size: 399 376 315 59 30 84 84 84
Cuts at: 2822 3081 3152 3243 3267 3291 3315 3430 3467
Size: 259 71 91 24 24 24 115 37
Cuts at: 3467 4209 4294 5365 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 376 315 259 115
91 85 84 84 84 71 59 37
30 24 24 24
BspEI T'CCGG_A
Cuts at: 5811 5811
Size: 6148
BspHI T'CATG_A
Cuts at: 4037 5767 4037
Size: 1730 4418
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2511 2595 2763 2847 3034 3503 844
Size: 1667 84 168 84 187 469 3489
Fragments arranged by size:
3489 1667 469 187 168 84 84
BsrBI CCG'CTC
Cuts at: 1394 1823 2608 2776 4035 1394
Size: 429 785 168 1259 3507
Fragments arranged by size:
3507 1259 785 429 168
BsrDI GCAATG_nn'
Cuts at: 65 1248 4643 4817 65
Size: 1183 3395 174 1396
Fragments arranged by size:
3395 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2990 3392 4792 4979 1700
Size: 110 1180 402 1400 187 2869
Fragments arranged by size:
2869 1400 1180 402 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6148
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6148
BssSI C'ACGA_G
Cuts at: 3363 4208 5220 3363
Size: 845 1012 4291
Fragments arranged by size:
4291 1012 845
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2948 794
Size: 2154 3994
BstBI TT'CG_AA
Cuts at: 4952 4952
Size: 6148
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6148
BstYI r'GATC_y
Cuts at: 1972 3160 3191 3478 3845 4230 4247 5699 5785
Size: 1188 31 287 367 385 17 1452 86
Cuts at: 5785 5797 6143 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1188 385 367 346 287 86
31 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2157 2311 2335 2522 2961 513
Size: 552 1092 154 24 187 439 3700
Fragments arranged by size:
3700 1092 552 439 187 154 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2234 2402 487
Size: 683 56 1008 168 4233
Fragments arranged by size:
4233 1008 683 168 56
BtsI GCAGTG_nn'
Cuts at: 2124 2449 2633 2710 2794 3428 3664 4458 4478
Size: 325 184 77 84 634 236 794 20
Cuts at: 4478 2124
Size: 3794
Fragments arranged by size:
3794 794 634 325 236 184 84 77
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6148
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6113
DraI TTT'AAA
Cuts at: 3452 3452
Size: 6148
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5155 809
Size: 507 3839 1802
Fragments arranged by size:
3839 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2145 2357 2560
Size: 54 1425 50 275 18 315 212 203
Cuts at: 2560 2685 2812 2896 2964 2985 3118 3595 4484
Size: 125 127 84 68 21 133 477 889
Cuts at: 4484 6113 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 477 315 275 212 203
133 127 125 84 68 54 50 43
21 18
EagI C'GGCC_G
Cuts at: 1537 1812 2357 2685 2964 2985 3595 1537
Size: 275 545 328 279 21 610 4090
Fragments arranged by size:
4090 610 545 328 279 275 21
EarI CTCTTCn'nnn_
Cuts at: 1353 4078 1353
Size: 2725 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2213 4735 5109 5255 387
Size: 1064 138 624 2522 374 146 1280
Fragments arranged by size:
2522 1280 1064 624 374 146 138
EcoICRI GAG'CTC
Cuts at: 2652 2736 2820 2652
Size: 84 84 5980
Fragments arranged by size:
5980 84 84
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2290 2816 2890 3172 4211 4808 5595
Size: 393 658 526 74 282 1039 597 787
Cuts at: 5595 5863 1239
Size: 268 1524
Fragments arranged by size:
1524 1039 787 658 597 526 393 282
268 74
EcoO109I rG'GnC_Cy
Cuts at: 3059 3437 3059
Size: 378 5770
EcoRI G'AATT_C
Cuts at: 3457 3835 3457
Size: 378 5770
FspI TGC'GCA
Cuts at: 2507 2591 2759 4654 2507
Size: 84 168 1895 4001
Fragments arranged by size:
4001 1895 168 84
HaeII r_GCGC'y
Cuts at: 1678 2401 2660 2695 2828 5295 1678
Size: 723 259 35 133 2467 2531
Fragments arranged by size:
2531 2467 723 259 133 35
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2047 2079 2096 2128 2233
Size: 32 135 32 475 32 17 32 105
Cuts at: 2233 2265 3122 3152 3154 3184 4789 4821 4863
Size: 32 857 30 2 30 1605 32 42
Cuts at: 4863 4895 1373
Size: 32 2626
Fragments arranged by size:
2626 1605 857 475 135 105 42 32
32 32 32 32 32 32 30 30
17 2
HincII GTy'rAC
Cuts at: 669 1657 1938 3492 3738 669
Size: 988 281 1554 246 3079
Fragments arranged by size:
3079 1554 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6148
HpaI GTT'AAC
Cuts at: 3738 3738
Size: 6148
KpnI G_GTAC'C
Cuts at: 3473 3473
Size: 6148
MfeI C'AATT_G
Cuts at: 3747 3747
Size: 6148
MluI A'CGCG_T
Cuts at: 6024 6024
Size: 6148
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2763 5262 5446 1305
Size: 1458 2499 184 2007
Fragments arranged by size:
2499 2007 1458 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2147 10
Size: 54 1425 343 315 4011
Fragments arranged by size:
4011 1425 343 315 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2937 4106 4465 4624 518
Size: 648 1771 1169 359 159 2042
Fragments arranged by size:
2042 1771 1169 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2159 2265 2283 2433 2524 2703 2948 2957
Size: 553 106 18 150 91 179 245 9
Cuts at: 2957 2963 2969 2975 2984 3564 4241 5389 5634
Size: 6 6 6 9 580 677 1148 245
Cuts at: 5634 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 580 553 245 245 179
150 106 91 18 9 9 6 6
6
NaeI GCC'GGC
Cuts at: 2992 2992
Size: 6148
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5596
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6148
NgoMIV G'CCGG_C
Cuts at: 2990 2990
Size: 6148
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6148
NotI GC'GGCC_GC
Cuts at: 2964 2985 3595 2964
Size: 21 610 5517
Fragments arranged by size:
5517 610 21
NspI r_CATG'y
Cuts at: 1132 1229 3506 5051 1132
Size: 97 2277 1545 2229
Fragments arranged by size:
2277 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6148
PciI A'CATG_T
Cuts at: 5047 5047
Size: 6148
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2143 3236 1108
Size: 1035 1093 4020
Fragments arranged by size:
4020 1093 1035
PmeI GTTT'AAAC
Cuts at: 3452 3452
Size: 6148
PmlI CAC'GTG
Cuts at: 2845 2845
Size: 6148
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4217 4249 5730 5762 4217
Size: 32 1481 32 4603
Fragments arranged by size:
4603 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3059 3059
Size: 6148
PshAI GACnn'nnGTC
Cuts at: 1613 3249 1613
Size: 1636 4512
PsiI TTA'TAA
Cuts at: 3718 3718
Size: 6148
PstI C_TGCA'G
Cuts at: 830 2667 2956 2974 2980 3500 830
Size: 1837 289 18 6 520 3478
Fragments arranged by size:
3478 1837 520 289 18 6
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6148
PvuII CAG'CTG
Cuts at: 2957 2975 2957
Size: 18 6130
SacI G_AGCT'C
Cuts at: 2654 2738 2822 2654
Size: 84 84 5980
Fragments arranged by size:
5980 84 84
SacII CC_GC'GG
Cuts at: 2160 2525 2964 2160
Size: 365 439 5344
Fragments arranged by size:
5344 439 365
SalI G'TCGA_C
Cuts at: 1936 3490 1936
Size: 1554 4594
SbfI CC_TGCA'GG
Cuts at: 3500 3500
Size: 6148
ScaI AGT'ACT
Cuts at: 4396 6108 4396
Size: 1712 4436
SfcI C'TryA_G
Cuts at: 826 1045 2663 2952 2970 2976 3496 4631 5312
Size: 219 1618 289 18 6 520 1135 681
Cuts at: 5312 5503 826
Size: 191 1471
Fragments arranged by size:
1618 1471 1135 681 520 289 219 191
18 6
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6148
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6148
SmaI CCC'GGG
Cuts at: 1872 3475 1872
Size: 1603 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4252 5153 5415 820
Size: 197 926 2309 901 262 1553
Fragments arranged by size:
2309 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3939 493
Size: 3446 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6148
SphI G_CATG'C
Cuts at: 1132 3506 1132
Size: 2374 3774
SspI AAT'ATT
Cuts at: 5 52 4072 5
Size: 47 4020 2081
Fragments arranged by size:
4020 2081 47
StuI AGG'CCT
Cuts at: 1517 2387 2471 2639 2807 2891 2903 1517
Size: 870 84 168 168 84 12 4762
Fragments arranged by size:
4762 870 168 168 84 84 12
StyI C'CwwG_G
Cuts at: 513 1065 2550 2802 3587 513
Size: 552 1485 252 785 3074
Fragments arranged by size:
3074 1485 785 552 252
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4175 4192 4345 4530 4975 325
Size: 1255 2595 17 153 185 445 1498
Fragments arranged by size:
2595 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4394 6106 96
Size: 274 80 33 51 1443 2417 1712 138
Fragments arranged by size:
2417 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6108 6140 6108
Size: 32 6116
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6148
XbaI T'CTAG_A
Cuts at: 3484 3484
Size: 6148
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6148
XmaI C'CCGG_G
Cuts at: 1870 3473 1870
Size: 1603 4545
XmnI GAAnn'nnTTC
Cuts at: 2303 2891 4277 2303
Size: 588 1386 4174
Fragments arranged by size:
4174 1386 588
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3956 276
Size: 53 83 186 936 2422 2468
Fragments arranged by size:
2468 2422 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV
FalI FseI FspAI KasI NarI NruI NsiI PacI
PfoI PspOMI PspXI PsrI RsrII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI