Restriction map for
pFN21AB9661
(Circular) (Six-base) MAPSORT of: pFN21AB9661.seq Check: 4709 from: 1 to: 5734
pFN21AB9661 5734 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3544 278
Size: 53 83 186 936 2008 2468
Fragments arranged by size:
2468 2008 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3077 1937
Size: 1140 4594
Acc65I G'GTAC_C
Cuts at: 3055 3055
Size: 5734
AclI AA'CG_TT
Cuts at: 1563 3861 4234 1563
Size: 2298 373 3063
Fragments arranged by size:
3063 2298 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3797 5181 5449 1632
Size: 2165 1384 268 1917
Fragments arranged by size:
2165 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5537
AflIII A'CryG_T
Cuts at: 1203 4633 5610 1203
Size: 3430 977 1327
Fragments arranged by size:
3430 1327 977
AgeI A'CCGG_T
Cuts at: 4565 4565
Size: 5734
AhdI GACnn_n'nnGTC
Cuts at: 4463 4463
Size: 5734
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5734
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2589 1184
Size: 1405 4329
AlwNI CAG_nnn'CTG
Cuts at: 3477 5049 3477
Size: 1572 4162
ApaI G_GGCC'C
Cuts at: 2549 2549
Size: 5734
ApaLI G'TGCA_C
Cuts at: 2047 2859 3791 4947 2047
Size: 812 932 1156 2834
Fragments arranged by size:
2834 1156 932 812
ApoI r'AATT_y
Cuts at: 1493 2657 3043 3269 3421 5530 1493
Size: 1164 386 226 152 2109 1697
Fragments arranged by size:
2109 1697 1164 386 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4288 160
Size: 872 3256 1606
Fragments arranged by size:
3256 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5734
AvaI C'yCGr_G
Cuts at: 1870 1943 2282 2561 3059 1870
Size: 73 339 279 498 4545
Fragments arranged by size:
4545 498 339 279 73
BamHI G'GATC_C
Cuts at: 3064 3431 3064
Size: 367 5367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3055 4510 618
Size: 325 217 627 1268 1455 1842
Fragments arranged by size:
1842 1455 1268 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2124 2283 2549 3053 1391
Size: 733 159 266 504 4072
Fragments arranged by size:
4072 733 504 266 159
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2216 2914 3009 928
Size: 971 317 698 95 3653
Fragments arranged by size:
3653 971 698 317 95
BbvCI CC'TCA_GC
Cuts at: 2435 2435
Size: 5734
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2840 2874 2999 3033 3925 3959 1903
Size: 34 903 34 125 34 892 34 3678
Fragments arranged by size:
3678 903 892 125 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2497 3626 4836 2497
Size: 1129 1210 3395
Fragments arranged by size:
3395 1210 1129
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5734
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4345 136
Size: 107 122 71 631 605 2673 1525
Fragments arranged by size:
2673 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2457 5729 2457
Size: 3272 2462
BlpI GC'TnA_GC
Cuts at: 3151 3151
Size: 5734
Bme1580I G_kGCm'C
Cuts at: 1790 2051 2549 2863 3795 4951 1790
Size: 261 498 314 932 1156 2573
Fragments arranged by size:
2573 1156 932 498 314 261
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5734
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2813 3465 4423
Size: 416 53 83 399 24 1396 652 958
Cuts at: 4423 442
Size: 1753
Fragments arranged by size:
1753 1396 958 652 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5734
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2007 4394 1239
Size: 768 2387 2579
Fragments arranged by size:
2579 2387 768
Bpu10I CC'TnA_GC
Cuts at: 2435 2435
Size: 5734
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2272 3859 4724 5022 2272
Size: 1587 865 298 2984
Fragments arranged by size:
2984 1587 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4397 882
Size: 617 2898 2219
Fragments arranged by size:
2898 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2573 3525 493
Size: 711 1369 952 2702
Fragments arranged by size:
2702 1369 952 711
BsaBI GATnn'nnATC
Cuts at: 5728 5728
Size: 5734
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2919 3541 3923 4558
Size: 53 83 186 936 1386 622 382 635
Cuts at: 4558 275
Size: 1451
Fragments arranged by size:
1451 1386 936 635 622 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2170 4167 4565 4839 4986 5397 2170
Size: 1997 398 274 147 411 2507
Fragments arranged by size:
2507 1997 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5509
Fragments arranged by size:
5509 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2444 1186
Size: 1258 4476
BseYI C'CCAG_C
Cuts at: 2586 4937 5433 2586
Size: 2351 496 2887
Fragments arranged by size:
2887 2351 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2140 2854 1163
Size: 573 404 714 4043
Fragments arranged by size:
4043 714 573 404
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2171 3184 3945 4973 1540
Size: 275 186 170 1013 761 1028 2301
Fragments arranged by size:
2301 1028 1013 761 275 186 170
BsiHKAI G_wGCw'C
Cuts at: 2051 2863 3795 3880 4951 2051
Size: 812 932 85 1071 2834
Fragments arranged by size:
2834 1071 932 812 85
BsmI GAATG_Cn'
Cuts at: 3245 3338 3245
Size: 93 5641
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2051 2124 2283 2549 2863 3053 3795
Size: 399 261 73 159 266 314 190 742
Cuts at: 3795 3880 4951 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 314 266 261 190
159 85 73
BspEI T'CCGG_A
Cuts at: 5397 5397
Size: 5734
BspHI T'CATG_A
Cuts at: 3623 5353 3623
Size: 1730 4004
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3089 844
Size: 2245 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3621 1394
Size: 429 1798 3507
Fragments arranged by size:
3507 1798 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2524 4229 4403 65
Size: 1183 1276 1705 174 1396
Fragments arranged by size:
1705 1396 1276 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4378 4565 1700
Size: 110 2568 187 2869
Fragments arranged by size:
2869 2568 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5734
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5734
BssSI C'ACGA_G
Cuts at: 3794 4806 3794
Size: 1012 4722
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5734
BstBI TT'CG_AA
Cuts at: 4538 4538
Size: 5734
BstEII G'GTnAC_C
Cuts at: 1184 2185 1184
Size: 1001 4733
BstYI r'GATC_y
Cuts at: 1972 2421 2457 3064 3431 3816 3833 5285 5371
Size: 449 36 607 367 385 17 1452 86
Cuts at: 5371 5383 5729 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 607 449 385 367 346 86
36 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2328 513
Size: 552 939 324 3919
Fragments arranged by size:
3919 939 552 324
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2403 2778 487
Size: 683 56 1177 375 3443
Fragments arranged by size:
3443 1177 683 375 56
BtsI GCAGTG_nn'
Cuts at: 2861 3250 4044 4064 2861
Size: 389 794 20 4531
Fragments arranged by size:
4531 794 389 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5734
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5699
DraI TTT'AAA
Cuts at: 3038 3038
Size: 5734
DraIII CAC_nnn'GTG
Cuts at: 2832 2832
Size: 5734
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4741 809
Size: 507 3425 1802
Fragments arranged by size:
3425 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3181 4070 5699
Size: 54 1425 50 275 18 1351 889 1629
Cuts at: 5699 8
Size: 43
Fragments arranged by size:
1629 1425 1351 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3181 1537
Size: 275 1369 4090
Fragments arranged by size:
4090 1369 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2256 2262 2530 3664 1353
Size: 903 6 268 1134 3423
Fragments arranged by size:
3423 1134 903 268 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2089 4321 4695 4841 387
Size: 1064 138 500 2232 374 146 1280
Fragments arranged by size:
2232 1280 1064 500 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2007 3797 4394 5181 5449 1239
Size: 393 375 1790 597 787 268 1524
Fragments arranged by size:
1790 1524 787 597 393 375 268
EcoO109I rG'GnC_Cy
Cuts at: 2545 2546 2545
Size: 1 5733
EcoRI G'AATT_C
Cuts at: 3043 3421 3043
Size: 378 5356
FspI TGC'GCA
Cuts at: 4240 4240
Size: 5734
HaeII r_GCGC'y
Cuts at: 1678 2104 2561 4881 1678
Size: 426 457 2320 2531
Fragments arranged by size:
2531 2320 457 426
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2179 2211 4375 4407 4449
Size: 32 135 32 607 32 2164 32 42
Cuts at: 4449 4481 1373
Size: 32 2626
Fragments arranged by size:
2626 2164 607 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3078 3324 669
Size: 988 281 1140 246 3079
Fragments arranged by size:
3079 1140 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5734
HpaI GTT'AAC
Cuts at: 3324 3324
Size: 5734
KpnI G_GTAC'C
Cuts at: 3059 3059
Size: 5734
MfeI C'AATT_G
Cuts at: 3333 3333
Size: 5734
MluI A'CGCG_T
Cuts at: 5610 5610
Size: 5734
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3015 4848 5032 1305
Size: 1710 1833 184 2007
Fragments arranged by size:
2007 1833 1710 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3912
Fragments arranged by size:
3912 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2571 2785 3692 4051 4210 518
Size: 648 1405 214 907 359 159 2042
Fragments arranged by size:
2042 1405 907 648 359 214 159
MspA1I CmG'CkG
Cuts at: 1606 2357 2652 3150 3827 4975 5220 1606
Size: 751 295 498 677 1148 245 2120
Fragments arranged by size:
2120 1148 751 677 498 295 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4243
Fragments arranged by size:
4243 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5734
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5734
NotI GC'GGCC_GC
Cuts at: 3181 3181
Size: 5734
NspI r_CATG'y
Cuts at: 1132 1229 3092 4637 1132
Size: 97 1863 1545 2229
Fragments arranged by size:
2229 1863 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5734
PciI A'CATG_T
Cuts at: 4633 4633
Size: 5734
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5734
PfoI T'CCnGG_A
Cuts at: 2023 2424 2528 2023
Size: 401 104 5229
Fragments arranged by size:
5229 401 104
PmeI GTTT'AAAC
Cuts at: 3038 3038
Size: 5734
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3803 3835 5316 5348 3803
Size: 32 1481 32 4189
Fragments arranged by size:
4189 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2357 1613
Size: 744 4990
PsiI TTA'TAA
Cuts at: 2304 3304 2304
Size: 1000 4734
PspOMI G'GGCC_C
Cuts at: 2545 2545
Size: 5734
PstI C_TGCA'G
Cuts at: 830 2868 3086 830
Size: 2038 218 3478
Fragments arranged by size:
3478 2038 218
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5734
PvuII CAG'CTG
Cuts at: 2357 2652 2357
Size: 295 5439
SalI G'TCGA_C
Cuts at: 1936 3076 1936
Size: 1140 4594
SbfI CC_TGCA'GG
Cuts at: 3086 3086
Size: 5734
ScaI AGT'ACT
Cuts at: 3982 5694 3982
Size: 1712 4022
SfcI C'TryA_G
Cuts at: 826 1045 2864 2970 3082 4217 4898 5089 826
Size: 219 1819 106 112 1135 681 191 1471
Fragments arranged by size:
1819 1471 1135 681 219 191 112 106
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5734
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5734
SmaI CCC'GGG
Cuts at: 1872 3061 1872
Size: 1189 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2251 3838 4739 5001 820
Size: 197 926 308 1587 901 262 1553
Fragments arranged by size:
1587 1553 926 901 308 262 197
SnaBI TAC'GTA
Cuts at: 493 3525 493
Size: 3032 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5734
SphI G_CATG'C
Cuts at: 1132 3092 1132
Size: 1960 3774
SspI AAT'ATT
Cuts at: 5 52 3658 5
Size: 47 3606 2081
Fragments arranged by size:
3606 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5734
StyI C'CwwG_G
Cuts at: 513 1065 2004 2499 3173 513
Size: 552 939 495 674 3074
Fragments arranged by size:
3074 939 674 552 495
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2679 3761 3778 3931 4116 4561 325
Size: 1255 1099 1082 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1099 1082 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3980 5692 96
Size: 274 80 33 51 1443 2003 1712 138
Fragments arranged by size:
2003 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2019 2030 2051 2062 5694 5726 2019
Size: 11 21 11 3632 32 2027
Fragments arranged by size:
3632 2027 32 21 11 11
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5734
XbaI T'CTAG_A
Cuts at: 3070 3070
Size: 5734
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2912 2912
Size: 5734
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5734
XmaI C'CCGG_G
Cuts at: 1870 3059 1870
Size: 1189 4545
XmnI GAAnn'nnTTC
Cuts at: 3863 3863
Size: 5734
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3542 276
Size: 53 83 186 936 2008 2468
Fragments arranged by size:
2468 2008 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI