Restriction map for
pFN21AB9658
(Circular) (Six-base) MAPSORT of: pFN21AB9658.seq Check: 915 from: 1 to: 5674
pFN21AB9658 5674 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2694 2821 2694
Size: 127 5547
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3484 278
Size: 53 83 186 936 1948 2468
Fragments arranged by size:
2468 1948 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3017 1937
Size: 1080 4594
Acc65I G'GTAC_C
Cuts at: 2995 2995
Size: 5674
AclI AA'CG_TT
Cuts at: 1563 3801 4174 1563
Size: 2238 373 3063
Fragments arranged by size:
3063 2238 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2206 2741 2762 2905 3737 5121 5389 1632
Size: 574 535 21 143 832 1384 268 1917
Fragments arranged by size:
1917 1384 832 574 535 268 143 21
AfeI AGC'GCT
Cuts at: 2334 2334
Size: 5674
AflII C'TTAA_G
Cuts at: 820 1017 2843 820
Size: 197 1826 3651
Fragments arranged by size:
3651 1826 197
AflIII A'CryG_T
Cuts at: 1203 2433 2779 4573 5550 1203
Size: 1230 346 1794 977 1327
Fragments arranged by size:
1794 1327 1230 977 346
AgeI A'CCGG_T
Cuts at: 4505 4505
Size: 5674
AhdI GACnn_n'nnGTC
Cuts at: 4403 4403
Size: 5674
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5674
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2165 1184
Size: 981 4693
AlwNI CAG_nnn'CTG
Cuts at: 2139 2365 3417 4989 2139
Size: 226 1052 1572 2824
Fragments arranged by size:
2824 1572 1052 226
ApaI G_GGCC'C
Cuts at: 2474 2574 2474
Size: 100 5574
ApaLI G'TGCA_C
Cuts at: 3731 4887 3731
Size: 1156 4518
ApoI r'AATT_y
Cuts at: 1493 2983 3209 3361 5470 1493
Size: 1490 226 152 2109 1697
Fragments arranged by size:
2109 1697 1490 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4228 160
Size: 872 3196 1606
Fragments arranged by size:
3196 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5674
AvaI C'yCGr_G
Cuts at: 1870 1943 2249 2294 2315 2440 2567 2573 2960
Size: 73 306 45 21 125 127 6 387
Cuts at: 2960 2999 1870
Size: 39 4545
Fragments arranged by size:
4545 387 306 127 125 73 45 39
21 6
BamHI G'GATC_C
Cuts at: 3004 3371 3004
Size: 367 5307
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2444 2995 4450 618
Size: 325 217 627 657 551 1455 1842
Fragments arranged by size:
1842 1455 657 627 551 325 217
BanII G_rGCy'C
Cuts at: 1391 2474 2574 2936 2993 1391
Size: 1083 100 362 57 4072
Fragments arranged by size:
4072 1083 362 100 57
BbeI G_GCGC'C
Cuts at: 2448 2448
Size: 5674
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2246 2819 928
Size: 971 347 573 3783
Fragments arranged by size:
3783 971 573 347
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3865 3899 1903
Size: 34 1928 34 3678
Fragments arranged by size:
3678 1928 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3566 4776 3566
Size: 1210 4464
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5674
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2113 2453 4285
Size: 107 122 71 631 605 441 340 1832
Cuts at: 4285 136
Size: 1525
Fragments arranged by size:
1832 1525 631 605 441 340 122 107
71
BglII A'GATC_T
Cuts at: 5669 5669
Size: 5674
BlpI GC'TnA_GC
Cuts at: 2018 2183 3091 2018
Size: 165 908 4601
Fragments arranged by size:
4601 908 165
Bme1580I G_kGCm'C
Cuts at: 1790 2208 2474 2574 3735 4891 1790
Size: 418 266 100 1161 1156 2573
Fragments arranged by size:
2573 1161 1156 418 266 100
BmgBI CAC'GTC
Cuts at: 1318 2095 1318
Size: 777 4897
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2120 2345 2755
Size: 416 53 83 399 24 703 225 410
Cuts at: 2755 3405 4363 442
Size: 650 958 1753
Fragments arranged by size:
1753 958 703 650 416 410 399 225
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2538 1056
Size: 1482 4192
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2950 2950
Size: 5674
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2822 4334 1239
Size: 1583 1512 2579
Fragments arranged by size:
2579 1583 1512
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2069 3799 4664 4962 2069
Size: 1730 865 298 2781
Fragments arranged by size:
2781 1730 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4337 882
Size: 617 2838 2219
Fragments arranged by size:
2838 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3465 493
Size: 711 2261 2702
Fragments arranged by size:
2702 2261 711
BsaBI GATnn'nnATC
Cuts at: 5668 5668
Size: 5674
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2312 2445 2880 3481
Size: 53 83 186 936 779 133 435 601
Cuts at: 3481 3863 4498 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 779 635 601 435 382 186
133 83 53
BsaWI w'CCGG_w
Cuts at: 4107 4505 4779 4926 5337 4107
Size: 398 274 147 411 4444
Fragments arranged by size:
4444 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2621 2651 2936 2966 1178
Size: 30 165 30 1218 30 285 30 3886
Fragments arranged by size:
3886 1218 285 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2944 1186
Size: 1758 3916
BseYI C'CCAG_C
Cuts at: 2216 2467 4877 5373 2216
Size: 251 2410 496 2517
Fragments arranged by size:
2517 2410 496 251
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2125 1163
Size: 573 389 4712
Fragments arranged by size:
4712 573 389
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2232 3124 3885 4913 1540
Size: 275 186 231 892 761 1028 2301
Fragments arranged by size:
2301 1028 892 761 275 231 186
BsiHKAI G_wGCw'C
Cuts at: 3735 3820 4891 3735
Size: 85 1071 4518
Fragments arranged by size:
4518 1071 85
BsmI GAATG_Cn'
Cuts at: 3185 3278 3185
Size: 93 5581
BsmBI CGTCTCn'nnnn_
Cuts at: 2871 2871
Size: 5674
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2208 2474 2574 2936 2993 3735 3820
Size: 399 418 266 100 362 57 742 85
Cuts at: 3820 4891 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 418 399 362 266 100
85 57
BspEI T'CCGG_A
Cuts at: 5337 5337
Size: 5674
BspHI T'CATG_A
Cuts at: 3563 5293 3563
Size: 1730 3944
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2088 2694 2760 2821 3029 844
Size: 1244 606 66 61 208 3489
Fragments arranged by size:
3489 1244 606 208 66 61
BsrBI CCG'CTC
Cuts at: 1394 1823 2182 3561 1394
Size: 429 359 1379 3507
Fragments arranged by size:
3507 1379 429 359
BsrDI GCAATG_nn'
Cuts at: 65 1248 4169 4343 65
Size: 1183 2921 174 1396
Fragments arranged by size:
2921 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2159 2447 4318 4505 1700
Size: 110 349 288 1871 187 2869
Fragments arranged by size:
2869 1871 349 288 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5674
BssHII G'CGCG_C
Cuts at: 1925 2116 1925
Size: 191 5483
BssSI C'ACGA_G
Cuts at: 2651 3734 4746 2651
Size: 1083 1012 3579
Fragments arranged by size:
3579 1083 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2139 2353 2551 794
Size: 1345 214 198 3917
Fragments arranged by size:
3917 1345 214 198
BstBI TT'CG_AA
Cuts at: 4478 4478
Size: 5674
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5674
BstXI CCAn_nnnn'nTGG
Cuts at: 2050 2050
Size: 5674
BstYI r'GATC_y
Cuts at: 1972 2254 3004 3371 3756 3773 5225 5311 5323
Size: 282 750 367 385 17 1452 86 12
Cuts at: 5323 5669 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 750 385 367 346 282 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2010 2055 513
Size: 552 939 6 45 4132
Fragments arranged by size:
4132 939 552 45 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4935
Fragments arranged by size:
4935 683 56
BtsI GCAGTG_nn'
Cuts at: 3190 3984 4004 3190
Size: 794 20 4860
Fragments arranged by size:
4860 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5674
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5639
DraI TTT'AAA
Cuts at: 2978 2978
Size: 5674
DraIII CAC_nnn'GTG
Cuts at: 2193 2353 2193
Size: 160 5514
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4681 809
Size: 507 3365 1802
Fragments arranged by size:
3365 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2052 3121
Size: 54 1425 50 275 18 177 45 1069
Cuts at: 3121 4010 5639 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1069 889 275 177 54 50
45 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3121 1537
Size: 275 1309 4090
Fragments arranged by size:
4090 1309 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3604 1353
Size: 2251 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2705 2749 4261 4635 4781 387
Size: 1064 138 1116 44 1512 374 146 1280
Fragments arranged by size:
1512 1280 1116 1064 374 146 138 44
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2206 2741 2762 2822 2905 3737 4334
Size: 393 574 535 21 60 83 832 597
Cuts at: 4334 5121 5389 1239
Size: 787 268 1524
Fragments arranged by size:
1524 832 787 597 574 535 393 268
83 60 21
EcoO109I rG'GnC_Cy
Cuts at: 2136 2136
Size: 5674
EcoRI G'AATT_C
Cuts at: 2983 3361 2983
Size: 378 5296
FspI TGC'GCA
Cuts at: 4180 4180
Size: 5674
HaeII r_GCGC'y
Cuts at: 1678 2303 2336 2394 2448 2532 4821 1678
Size: 625 33 58 54 84 2289 2531
Fragments arranged by size:
2531 2289 625 84 58 54 33
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2621 2653 2936 2968 4315
Size: 32 135 32 1049 32 283 32 1347
Cuts at: 4315 4347 4389 4421 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1347 1049 283 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2068 3018 3264 669
Size: 988 281 130 950 246 3079
Fragments arranged by size:
3079 988 950 281 246 130
HindIII A'AGCT_T
Cuts at: 748 2371 748
Size: 1623 4051
HpaI GTT'AAC
Cuts at: 3264 3264
Size: 5674
KasI G'GCGC_C
Cuts at: 2444 2444
Size: 5674
KpnI G_GTAC'C
Cuts at: 2999 2999
Size: 5674
MfeI C'AATT_G
Cuts at: 3273 3273
Size: 5674
MluI A'CGCG_T
Cuts at: 2433 2779 5550 2433
Size: 346 2771 2557
Fragments arranged by size:
2771 2557 346
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2664 4788 4972 1305
Size: 1359 2124 184 2007
Fragments arranged by size:
2124 2007 1359 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2054 10
Size: 54 1425 343 222 3630
Fragments arranged by size:
3630 1425 343 222 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2663 3632 3991 4150 518
Size: 648 1497 969 359 159 2042
Fragments arranged by size:
2042 1497 969 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2012 2050 2365 2551 3090 3767 4915 5160
Size: 406 38 315 186 539 677 1148 245
Cuts at: 5160 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 539 406 315 245 186
38
NaeI GCC'GGC
Cuts at: 2161 2449 2161
Size: 288 5386
NarI GG'CG_CC
Cuts at: 2445 2445
Size: 5674
NcoI C'CATG_G
Cuts at: 513 1065 2004 2055 513
Size: 552 939 51 4132
Fragments arranged by size:
4132 939 552 51
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5674
NgoMIV G'CCGG_C
Cuts at: 2159 2447 2159
Size: 288 5386
NheI G'CTAG_C
Cuts at: 1052 2534 1052
Size: 1482 4192
NotI GC'GGCC_GC
Cuts at: 3121 3121
Size: 5674
NspI r_CATG'y
Cuts at: 1132 1229 3032 4577 1132
Size: 97 1803 1545 2229
Fragments arranged by size:
2229 1803 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5674
PciI A'CATG_T
Cuts at: 4573 4573
Size: 5674
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5674
PmeI GTTT'AAAC
Cuts at: 2978 2978
Size: 5674
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3743 3775 5256 5288 3743
Size: 32 1481 32 4129
Fragments arranged by size:
4129 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5674
PsiI TTA'TAA
Cuts at: 3244 3244
Size: 5674
PspOMI G'GGCC_C
Cuts at: 2470 2570 2470
Size: 100 5574
PspXI vC'TCGA_Gb
Cuts at: 2249 2249
Size: 5674
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2738 2770 2738
Size: 32 5642
PstI C_TGCA'G
Cuts at: 830 2154 3026 830
Size: 1324 872 3478
Fragments arranged by size:
3478 1324 872
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5674
PvuII CAG'CTG
Cuts at: 2551 2551
Size: 5674
SacII CC_GC'GG
Cuts at: 2013 2013
Size: 5674
SalI G'TCGA_C
Cuts at: 1936 3016 1936
Size: 1080 4594
SbfI CC_TGCA'GG
Cuts at: 3026 3026
Size: 5674
ScaI AGT'ACT
Cuts at: 3922 5634 3922
Size: 1712 3962
SfcI C'TryA_G
Cuts at: 826 1045 2150 3022 4157 4838 5029 826
Size: 219 1105 872 1135 681 191 1471
Fragments arranged by size:
1471 1135 1105 872 681 219 191
SfoI GGC'GCC
Cuts at: 2446 2446
Size: 5674
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5674
SgrAI Cr'CCGG_yG
Cuts at: 1700 2447 1700
Size: 747 4927
SmaI CCC'GGG
Cuts at: 1872 2442 2569 2575 3001 1872
Size: 570 127 6 426 4545
Fragments arranged by size:
4545 570 426 127 6
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2084 2249 2843 3778 4679 4941
Size: 197 926 141 165 594 935 901 262
Cuts at: 4941 820
Size: 1553
Fragments arranged by size:
1553 935 926 901 594 262 197 165
141
SnaBI TAC'GTA
Cuts at: 493 3465 493
Size: 2972 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5674
SphI G_CATG'C
Cuts at: 1132 3032 1132
Size: 1900 3774
SrfI GCCC'GGGC
Cuts at: 2575 2575
Size: 5674
SspI AAT'ATT
Cuts at: 5 52 3598 5
Size: 47 3546 2081
Fragments arranged by size:
3546 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5674
StyI C'CwwG_G
Cuts at: 513 1065 2004 2055 2700 3113 513
Size: 552 939 51 645 413 3074
Fragments arranged by size:
3074 939 645 552 413 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2246 2364 3701 3718 3871 4056 4501
Size: 1255 666 118 1337 17 153 185 445
Cuts at: 4501 325
Size: 1498
Fragments arranged by size:
1498 1337 1255 666 445 185 153 118
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2259 3920 5632
Size: 274 80 33 51 1443 282 1661 1712
Cuts at: 5632 96
Size: 138
Fragments arranged by size:
1712 1661 1443 282 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5634 5666 5634
Size: 32 5642
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5674
XbaI T'CTAG_A
Cuts at: 3010 3010
Size: 5674
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2047 2047
Size: 5674
XhoI C'TCGA_G
Cuts at: 1943 2249 1943
Size: 306 5368
XmaI C'CCGG_G
Cuts at: 1870 2440 2567 2573 2999 1870
Size: 570 127 6 426 4545
Fragments arranged by size:
4545 570 426 127 6
XmnI GAAnn'nnTTC
Cuts at: 3803 3803
Size: 5674
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3482 276
Size: 53 83 186 936 1948 2468
Fragments arranged by size:
2468 1948 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI PvuII SacII SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI
BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI
NruI NsiI PacI PfoI PmlI PpuMI RsrII SacI
SanDI SapI SexAI SfiI SwaI