Restriction map for
pFN21AB9652
(Circular) (Six-base) MAPSORT of: pFN21AB9652.seq Check: 6441 from: 1 to: 5656
pFN21AB9652 5656 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3466 278
Size: 53 83 186 936 1930 2468
Fragments arranged by size:
2468 1930 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2999 1937
Size: 1062 4594
Acc65I G'GTAC_C
Cuts at: 2977 2977
Size: 5656
AclI AA'CG_TT
Cuts at: 1563 3783 4156 1563
Size: 2220 373 3063
Fragments arranged by size:
3063 2220 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2051 3719 5103 5371 1632
Size: 419 1668 1384 268 1917
Fragments arranged by size:
1917 1668 1384 419 268
AfeI AGC'GCT
Cuts at: 2301 2301
Size: 5656
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5459
AflIII A'CryG_T
Cuts at: 1203 2883 4555 5532 1203
Size: 1680 1672 977 1327
Fragments arranged by size:
1680 1672 1327 977
AgeI A'CCGG_T
Cuts at: 4487 4487
Size: 5656
AhdI GACnn_n'nnGTC
Cuts at: 4385 4385
Size: 5656
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5656
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5656
AlwNI CAG_nnn'CTG
Cuts at: 3399 4971 3399
Size: 1572 4084
ApaI G_GGCC'C
Cuts at: 2045 2045
Size: 5656
ApaLI G'TGCA_C
Cuts at: 3713 4869 3713
Size: 1156 4500
ApoI r'AATT_y
Cuts at: 1493 2965 3191 3343 5452 1493
Size: 1472 226 152 2109 1697
Fragments arranged by size:
2109 1697 1472 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4210 160
Size: 872 3178 1606
Fragments arranged by size:
3178 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5656
AvaI C'yCGr_G
Cuts at: 1870 1943 2554 2981 1870
Size: 73 611 427 4545
Fragments arranged by size:
4545 611 427 73
BamHI G'GATC_C
Cuts at: 2986 3353 2986
Size: 367 5289
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2231 2441 2696 2791 2843
Size: 325 217 627 444 210 255 95 52
Cuts at: 2843 2851 2911 2977 4432 618
Size: 8 60 66 1455 1842
Fragments arranged by size:
1842 1455 627 444 325 255 217 210
95 66 60 52 8
BanII G_rGCy'C
Cuts at: 1391 2045 2975 1391
Size: 654 930 4072
Fragments arranged by size:
4072 930 654
BbeI G_GCGC'C
Cuts at: 2235 2445 2795 2847 2235
Size: 210 350 52 5044
Fragments arranged by size:
5044 350 210 52
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4685
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3847 3881 1903
Size: 34 1910 34 3678
Fragments arranged by size:
3678 1910 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3548 4758 3548
Size: 1210 4446
BclI T'GATC_A
Cuts at: 1548 2823 1548
Size: 1275 4381
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2392 4267 136
Size: 107 122 71 631 605 720 1875 1525
Fragments arranged by size:
1875 1525 720 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5651 5651
Size: 5656
BlpI GC'TnA_GC
Cuts at: 2286 3073 2286
Size: 787 4869
Bme1580I G_kGCm'C
Cuts at: 1790 2045 2699 2916 3717 4873 1790
Size: 255 654 217 801 1156 2573
Fragments arranged by size:
2573 1156 801 654 255 217
BmgBI CAC'GTC
Cuts at: 1318 2608 1318
Size: 1290 4366
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3387 4345 442
Size: 416 53 83 399 24 1970 958 1753
Fragments arranged by size:
1970 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5656
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2767 2767
Size: 5656
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2521 2737 2770 4316 1239
Size: 1282 216 33 1546 2579
Fragments arranged by size:
2579 1546 1282 216 33
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3781 4646 4944 3781
Size: 865 298 4493
Fragments arranged by size:
4493 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4319 882
Size: 617 2820 2219
Fragments arranged by size:
2820 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3447 493
Size: 711 2243 2702
Fragments arranged by size:
2702 2243 711
BsaBI GATnn'nnATC
Cuts at: 5650 5650
Size: 5656
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2232 2442 2792 2844
Size: 53 83 186 936 699 210 350 52
Cuts at: 2844 3463 3845 4480 275
Size: 619 382 635 1451
Fragments arranged by size:
1451 936 699 635 619 382 350 210
186 83 53 52
BsaWI w'CCGG_w
Cuts at: 4089 4487 4761 4908 5319 4089
Size: 398 274 147 411 4426
Fragments arranged by size:
4426 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5431
Fragments arranged by size:
5431 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5656
BseYI C'CCAG_C
Cuts at: 2536 2870 2889 4859 5355 2536
Size: 334 19 1970 496 2837
Fragments arranged by size:
2837 1970 496 334 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2288 1163
Size: 573 552 4531
Fragments arranged by size:
4531 573 552
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2094 2270 3106 3867 4895 1540
Size: 275 186 93 176 836 761 1028 2301
Fragments arranged by size:
2301 1028 836 761 275 186 176 93
BsiHKAI G_wGCw'C
Cuts at: 2319 3717 3802 4873 2319
Size: 1398 85 1071 3102
Fragments arranged by size:
3102 1398 1071 85
BsmI GAATG_Cn'
Cuts at: 2146 3167 3260 2146
Size: 1021 93 4542
Fragments arranged by size:
4542 1021 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2015 2249 2015
Size: 234 5422
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2045 2319 2699 2916 2975 3717 3802
Size: 399 255 274 380 217 59 742 85
Cuts at: 3802 4873 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 380 274 255 217
85 59
BspEI T'CCGG_A
Cuts at: 5319 5319
Size: 5656
BspHI T'CATG_A
Cuts at: 2361 3545 5275 2361
Size: 1184 1730 2742
Fragments arranged by size:
2742 1730 1184
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3011 844
Size: 2167 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2427 2904 3543 1394
Size: 429 604 477 639 3507
Fragments arranged by size:
3507 639 604 477 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4151 4325 65
Size: 1183 2903 174 1396
Fragments arranged by size:
2903 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2093 2334 2444 4300 4487 1700
Size: 110 283 241 110 1856 187 2869
Fragments arranged by size:
2869 1856 283 241 187 110 110
BsrGI T'GTAC_A
Cuts at: 96 2007 96
Size: 1911 3745
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5656
BssSI C'ACGA_G
Cuts at: 3716 4728 3716
Size: 1012 4644
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2593 794
Size: 1799 3857
BstBI TT'CG_AA
Cuts at: 4460 4460
Size: 5656
BstEII G'GTnAC_C
Cuts at: 1184 2767 1184
Size: 1583 4073
BstYI r'GATC_y
Cuts at: 1972 2986 3353 3738 3755 5207 5293 5305 5651
Size: 1014 367 385 17 1452 86 12 346
Cuts at: 5651 1972
Size: 1977
Fragments arranged by size:
1977 1452 1014 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2082 2161 2721 513
Size: 552 1017 79 560 3448
Fragments arranged by size:
3448 1017 560 552 79
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4917
Fragments arranged by size:
4917 683 56
BtsI GCAGTG_nn'
Cuts at: 2936 3172 3966 3986 2936
Size: 236 794 20 4606
Fragments arranged by size:
4606 794 236 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5656
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5621
DraI TTT'AAA
Cuts at: 2960 2960
Size: 5656
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4663 809
Size: 507 3347 1802
Fragments arranged by size:
3347 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2091 2267 2922
Size: 54 1425 50 275 18 261 176 655
Cuts at: 2922 3103 3992 5621 8
Size: 181 889 1629 43
Fragments arranged by size:
1629 1425 889 655 275 261 181 176
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2091 2267 3103 1537
Size: 275 279 176 836 4090
Fragments arranged by size:
4090 836 279 275 176
EarI CTCTTCn'nnn_
Cuts at: 1353 2779 3586 1353
Size: 1426 807 3423
Fragments arranged by size:
3423 1426 807
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2359 4243 4617 4763 387
Size: 1064 138 770 1884 374 146 1280
Fragments arranged by size:
1884 1280 1064 770 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2051 2521 2737 2770 3719 4316 5103
Size: 393 419 470 216 33 949 597 787
Cuts at: 5103 5371 1239
Size: 268 1524
Fragments arranged by size:
1524 949 787 597 470 419 393 268
216 33
EcoO109I rG'GnC_Cy
Cuts at: 2809 2809
Size: 5656
EcoRI G'AATT_C
Cuts at: 2965 3343 2965
Size: 378 5278
FspI TGC'GCA
Cuts at: 2568 4162 2568
Size: 1594 4062
FspAI rTGC'GCAy
Cuts at: 2568 2568
Size: 5656
HaeII r_GCGC'y
Cuts at: 1678 2227 2235 2303 2445 2616 2795 2847 4803
Size: 549 8 68 142 171 179 52 1956
Cuts at: 4803 1678
Size: 2531
Fragments arranged by size:
2531 1956 549 179 171 142 68 52
8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4297 4329 4371 4403 1373
Size: 32 135 32 2725 32 42 32 2626
Fragments arranged by size:
2725 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3000 3246 669
Size: 988 281 1062 246 3079
Fragments arranged by size:
3079 1062 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5656
HpaI GTT'AAC
Cuts at: 3246 3246
Size: 5656
KasI G'GCGC_C
Cuts at: 2231 2441 2791 2843 2231
Size: 210 350 52 5044
Fragments arranged by size:
5044 350 210 52
KpnI G_GTAC'C
Cuts at: 2981 2981
Size: 5656
MfeI C'AATT_G
Cuts at: 3255 3255
Size: 5656
MluI A'CGCG_T
Cuts at: 5532 5532
Size: 5656
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2232 4770 4954 1305
Size: 927 2538 184 2007
Fragments arranged by size:
2538 2007 927 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3834
Fragments arranged by size:
3834 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3614 3973 4132 518
Size: 648 2448 359 159 2042
Fragments arranged by size:
2448 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2084 2163 2170 2536 3072 3749 4897 5142
Size: 478 79 7 366 536 677 1148 245
Cuts at: 5142 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 536 478 366 245 79
7
NaeI GCC'GGC
Cuts at: 2095 2446 2095
Size: 351 5305
NarI GG'CG_CC
Cuts at: 2232 2442 2792 2844 2232
Size: 210 350 52 5044
Fragments arranged by size:
5044 350 210 52
NcoI C'CATG_G
Cuts at: 513 1065 2721 513
Size: 552 1656 3448
Fragments arranged by size:
3448 1656 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5656
NgoMIV G'CCGG_C
Cuts at: 2093 2444 2093
Size: 351 5305
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5656
NotI GC'GGCC_GC
Cuts at: 3103 3103
Size: 5656
NspI r_CATG'y
Cuts at: 1132 1229 2680 2833 2887 3014 4559 1132
Size: 97 1451 153 54 127 1545 2229
Fragments arranged by size:
2229 1545 1451 153 127 97 54
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5656
PciI A'CATG_T
Cuts at: 2883 4555 2883
Size: 1672 3984
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2473 1108
Size: 1365 4291
PfoI T'CCnGG_A
Cuts at: 2814 2814
Size: 5656
PmeI GTTT'AAAC
Cuts at: 2960 2960
Size: 5656
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3725 3757 5238 5270 3725
Size: 32 1481 32 4111
Fragments arranged by size:
4111 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2809 2809
Size: 5656
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5656
PsiI TTA'TAA
Cuts at: 2328 3226 2328
Size: 898 4758
PspOMI G'GGCC_C
Cuts at: 2041 2041
Size: 5656
PstI C_TGCA'G
Cuts at: 830 2622 3008 830
Size: 1792 386 3478
Fragments arranged by size:
3478 1792 386
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5656
PvuII CAG'CTG
Cuts at: 2536 2536
Size: 5656
RsrII CG'GwC_CG
Cuts at: 2119 2119
Size: 5656
SacII CC_GC'GG
Cuts at: 2085 2164 2085
Size: 79 5577
SalI G'TCGA_C
Cuts at: 1936 2998 1936
Size: 1062 4594
SbfI CC_TGCA'GG
Cuts at: 3008 3008
Size: 5656
ScaI AGT'ACT
Cuts at: 3904 5616 3904
Size: 1712 3944
SfcI C'TryA_G
Cuts at: 826 1045 2515 2618 2671 3004 4139 4820 5011
Size: 219 1470 103 53 333 1135 681 191
Cuts at: 5011 826
Size: 1471
Fragments arranged by size:
1471 1470 1135 681 333 219 191 103
53
SfoI GGC'GCC
Cuts at: 2233 2443 2793 2845 2233
Size: 210 350 52 5044
Fragments arranged by size:
5044 350 210 52
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5656
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5656
SmaI CCC'GGG
Cuts at: 1872 2556 2983 1872
Size: 684 427 4545
Fragments arranged by size:
4545 684 427
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3760 4661 4923 820
Size: 197 926 1817 901 262 1553
Fragments arranged by size:
1817 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3447 493
Size: 2954 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5656
SphI G_CATG'C
Cuts at: 1132 3014 1132
Size: 1882 3774
SspI AAT'ATT
Cuts at: 5 52 3580 5
Size: 47 3528 2081
Fragments arranged by size:
3528 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5656
StyI C'CwwG_G
Cuts at: 513 1065 2721 3095 513
Size: 552 1656 374 3074
Fragments arranged by size:
3074 1656 552 374
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3683 3700 3853 4038 4483 325
Size: 1255 2103 17 153 185 445 1498
Fragments arranged by size:
2103 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2007 2376 2622
Size: 274 80 33 51 1443 30 369 246
Cuts at: 2622 3902 5614 96
Size: 1280 1712 138
Fragments arranged by size:
1712 1443 1280 369 274 246 138 80
51 33 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5616 5648 5616
Size: 32 5624
Tth111I GACn'n_nGTC
Cuts at: 1316 2123 1316
Size: 807 4849
XbaI T'CTAG_A
Cuts at: 2992 2992
Size: 5656
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2728 2761 2728
Size: 33 5623
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5656
XmaI C'CCGG_G
Cuts at: 1870 2554 2981 1870
Size: 684 427 4545
Fragments arranged by size:
4545 684 427
XmnI GAAnn'nnTTC
Cuts at: 2144 3785 2144
Size: 1641 4015
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3464 276
Size: 53 83 186 936 1930 2468
Fragments arranged by size:
2468 1930 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI FspAI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII RsrII SacII SalI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I
BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FalI FseI NruI NsiI PacI PmlI PspXI PsrI
SacI SanDI SapI SexAI SfiI SrfI SwaI