Restriction map for
pFN21AB9649
(Circular) (Six-base) MAPSORT of: pFN21AB9649.seq Check: 2962 from: 1 to: 6028
pFN21AB9649 6028 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3838 278
Size: 53 83 186 936 2302 2468
Fragments arranged by size:
2468 2302 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3371 1937
Size: 1434 4594
Acc65I G'GTAC_C
Cuts at: 3349 3349
Size: 6028
AclI AA'CG_TT
Cuts at: 1563 4155 4528 1563
Size: 2592 373 3063
Fragments arranged by size:
3063 2592 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2294 4091 5475 5743 1632
Size: 662 1797 1384 268 1917
Fragments arranged by size:
1917 1797 1384 662 268
AfeI AGC'GCT
Cuts at: 2261 2261
Size: 6028
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5831
AflIII A'CryG_T
Cuts at: 1203 2166 4927 5904 1203
Size: 963 2761 977 1327
Fragments arranged by size:
2761 1327 977 963
AgeI A'CCGG_T
Cuts at: 4859 4859
Size: 6028
AhdI GACnn_n'nnGTC
Cuts at: 4757 4757
Size: 6028
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6028
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6028
AlwNI CAG_nnn'CTG
Cuts at: 2770 2803 3145 3771 5343 2770
Size: 33 342 626 1572 3455
Fragments arranged by size:
3455 1572 626 342 33
ApaLI G'TGCA_C
Cuts at: 4085 5241 4085
Size: 1156 4872
ApoI r'AATT_y
Cuts at: 1493 2078 3106 3337 3563 3715 5824 1493
Size: 585 1028 231 226 152 2109 1697
Fragments arranged by size:
2109 1697 1028 585 231 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4582 160
Size: 872 3550 1606
Fragments arranged by size:
3550 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6028
AvaI C'yCGr_G
Cuts at: 1870 1943 2049 2626 2867 2911 3353 1870
Size: 73 106 577 241 44 442 4545
Fragments arranged by size:
4545 577 442 241 106 73 44
BamHI G'GATC_C
Cuts at: 3358 3725 3358
Size: 367 5661
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2471 2534 2915 3349 4804
Size: 325 217 627 684 63 381 434 1455
Cuts at: 4804 618
Size: 1842
Fragments arranged by size:
1842 1455 684 627 434 381 325 217
63
BanII G_rGCy'C
Cuts at: 1391 2417 2492 3230 3347 1391
Size: 1026 75 738 117 4072
Fragments arranged by size:
4072 1026 738 117 75
BbeI G_GCGC'C
Cuts at: 2538 2919 2538
Size: 381 5647
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5057
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4219 4253 1903
Size: 34 2282 34 3678
Fragments arranged by size:
3678 2282 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3920 5130 3920
Size: 1210 4818
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6028
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2525 2782 4639
Size: 107 122 71 631 605 853 257 1857
Cuts at: 4639 136
Size: 1525
Fragments arranged by size:
1857 1525 853 631 605 257 122 107
71
BglII A'GATC_T
Cuts at: 2238 6023 2238
Size: 3785 2243
BlpI GC'TnA_GC
Cuts at: 2846 3266 3445 2846
Size: 420 179 5429
Fragments arranged by size:
5429 420 179
Bme1580I G_kGCm'C
Cuts at: 1790 2832 4089 5245 1790
Size: 1042 1257 1156 2573
Fragments arranged by size:
2573 1257 1156 1042
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6028
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2557 3759 4717
Size: 416 53 83 399 24 1140 1202 958
Cuts at: 4717 442
Size: 1753
Fragments arranged by size:
1753 1202 1140 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6028
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2897 2897
Size: 6028
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2476 3314 4688 1239
Size: 1237 838 1374 2579
Fragments arranged by size:
2579 1374 1237 838
Bpu10I CC'TnA_GC
Cuts at: 3098 3098
Size: 6028
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2309 2498 4153 5018 5316 2309
Size: 189 1655 865 298 3021
Fragments arranged by size:
3021 1655 865 298 189
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4691 882
Size: 617 3192 2219
Fragments arranged by size:
3192 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2599 3819 493
Size: 711 1395 1220 2702
Fragments arranged by size:
2702 1395 1220 711
BsaBI GATnn'nnATC
Cuts at: 6022 6022
Size: 6028
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2138 2535 2916 3075
Size: 53 83 186 936 605 397 381 159
Cuts at: 3075 3835 4217 4852 275
Size: 760 382 635 1451
Fragments arranged by size:
1451 936 760 635 605 397 382 381
186 159 83 53
BsaWI w'CCGG_w
Cuts at: 2218 4461 4859 5133 5280 5691 2218
Size: 2243 398 274 147 411 2555
Fragments arranged by size:
2555 2243 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5803
Fragments arranged by size:
5803 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2084 2753 3019 1186
Size: 898 669 266 4195
Fragments arranged by size:
4195 898 669 266
BseYI C'CCAG_C
Cuts at: 2251 2399 2602 3145 5231 5727 2251
Size: 148 203 543 2086 496 2552
Fragments arranged by size:
2552 2086 543 496 203 148
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2030 3238 1163
Size: 573 294 1208 3953
Fragments arranged by size:
3953 1208 573 294
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2159 2433 2529 3165 3478 4239
Size: 275 186 158 274 96 636 313 761
Cuts at: 4239 5267 1540
Size: 1028 2301
Fragments arranged by size:
2301 1028 761 636 313 275 274 186
158 96
BsiHKAI G_wGCw'C
Cuts at: 4089 4174 5245 4089
Size: 85 1071 4872
Fragments arranged by size:
4872 1071 85
BsmI GAATG_Cn'
Cuts at: 3539 3632 3539
Size: 93 5935
BsmBI CGTCTCn'nnnn_
Cuts at: 2800 2800
Size: 6028
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2417 2492 2832 3230 3347 4089 4174
Size: 399 627 75 340 398 117 742 85
Cuts at: 4174 5245 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 627 399 398 340 117
85 75
BspEI T'CCGG_A
Cuts at: 2218 5691 2218
Size: 3473 2555
BspHI T'CATG_A
Cuts at: 3917 5647 3917
Size: 1730 4298
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2052 3383 844
Size: 1208 1331 3489
Fragments arranged by size:
3489 1331 1208
BsrBI CCG'CTC
Cuts at: 1394 1823 2314 2622 2980 3915 1394
Size: 429 491 308 358 935 3507
Fragments arranged by size:
3507 935 491 429 358 308
BsrDI GCAATG_nn'
Cuts at: 65 1248 4523 4697 65
Size: 1183 3275 174 1396
Fragments arranged by size:
3275 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2358 2432 2900 4672 4859 1700
Size: 110 548 74 468 1772 187 2869
Fragments arranged by size:
2869 1772 548 468 187 110 74
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6028
BssHII G'CGCG_C
Cuts at: 1925 3120 1925
Size: 1195 4833
BssSI C'ACGA_G
Cuts at: 4088 5100 4088
Size: 1012 5016
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2502 2770 794
Size: 1708 268 4052
Fragments arranged by size:
4052 1708 268
BstBI TT'CG_AA
Cuts at: 4832 4832
Size: 6028
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6028
BstXI CCAn_nnnn'nTGG
Cuts at: 2251 2251
Size: 6028
BstYI r'GATC_y
Cuts at: 1972 2238 2289 3358 3725 4110 4127 5579 5665
Size: 266 51 1069 367 385 17 1452 86
Cuts at: 5665 5677 6023 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1069 385 367 346 266 86
51 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2523 2529 513
Size: 552 939 519 6 4012
Fragments arranged by size:
4012 939 552 519 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5289
Fragments arranged by size:
5289 683 56
BtsI GCAGTG_nn'
Cuts at: 3544 4338 4358 3544
Size: 794 20 5214
Fragments arranged by size:
5214 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6028
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5993
DraI TTT'AAA
Cuts at: 3332 3332
Size: 6028
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5035 809
Size: 507 3719 1802
Fragments arranged by size:
3719 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2156 2334 2391
Size: 54 1425 50 275 18 326 178 57
Cuts at: 2391 2430 2526 3162 3475 4364 5993 8
Size: 39 96 636 313 889 1629 43
Fragments arranged by size:
1629 1425 889 636 326 313 275 178
96 57 54 50 43 39 18
EagI C'GGCC_G
Cuts at: 1537 1812 2156 2430 2526 3162 3475 1537
Size: 275 344 274 96 636 313 4090
Fragments arranged by size:
4090 636 344 313 275 274 96
EarI CTCTTCn'nnn_
Cuts at: 1353 2417 3958 1353
Size: 1064 1541 3423
Fragments arranged by size:
3423 1541 1064
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2033 2324 2472 2873 4615 4989
Size: 1064 138 444 291 148 401 1742 374
Cuts at: 4989 5135 387
Size: 146 1280
Fragments arranged by size:
1742 1280 1064 444 401 374 291 148
146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2294 2476 3314 4091 4688 5475 5743
Size: 393 662 182 838 777 597 787 268
Cuts at: 5743 1239
Size: 1524
Fragments arranged by size:
1524 838 787 777 662 597 393 268
182
EcoO109I rG'GnC_Cy
Cuts at: 2518 2518
Size: 6028
EcoRI G'AATT_C
Cuts at: 2078 3337 3715 2078
Size: 1259 378 4391
Fragments arranged by size:
4391 1259 378
FspI TGC'GCA
Cuts at: 4534 4534
Size: 6028
HaeII r_GCGC'y
Cuts at: 1678 2060 2263 2406 2538 2919 3132 5175 1678
Size: 382 203 143 132 381 213 2043 2531
Fragments arranged by size:
2531 2043 382 381 213 203 143 132
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4669 4701 4743 4775 1373
Size: 32 135 32 3097 32 42 32 2626
Fragments arranged by size:
3097 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3372 3618 669
Size: 988 281 1434 246 3079
Fragments arranged by size:
3079 1434 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6028
HpaI GTT'AAC
Cuts at: 3618 3618
Size: 6028
KasI G'GCGC_C
Cuts at: 2534 2915 2534
Size: 381 5647
KpnI G_GTAC'C
Cuts at: 3353 3353
Size: 6028
MfeI C'AATT_G
Cuts at: 3627 3627
Size: 6028
MluI A'CGCG_T
Cuts at: 2166 5904 2166
Size: 3738 2290
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3333 5142 5326 1305
Size: 2028 1809 184 2007
Fragments arranged by size:
2028 2007 1809 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4206
Fragments arranged by size:
4206 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3986 4345 4504 518
Size: 648 2820 359 159 2042
Fragments arranged by size:
2820 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2525 2531 2713 2800 3045 3057 3066 3145
Size: 919 6 182 87 245 12 9 79
Cuts at: 3145 3444 4121 5269 5514 1606
Size: 299 677 1148 245 2120
Fragments arranged by size:
2120 1148 919 677 299 245 245 182
87 79 12 9 6
NaeI GCC'GGC
Cuts at: 2434 2902 2434
Size: 468 5560
NarI GG'CG_CC
Cuts at: 2535 2916 2535
Size: 381 5647
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4537
Fragments arranged by size:
4537 939 552
NdeI CA'TA_TG
Cuts at: 387 3325 387
Size: 2938 3090
NgoMIV G'CCGG_C
Cuts at: 2432 2900 2432
Size: 468 5560
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6028
NotI GC'GGCC_GC
Cuts at: 2526 3475 2526
Size: 949 5079
NspI r_CATG'y
Cuts at: 1132 1229 2227 3386 4931 1132
Size: 97 998 1159 1545 2229
Fragments arranged by size:
2229 1545 1159 998 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6028
PciI A'CATG_T
Cuts at: 4927 4927
Size: 6028
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6028
PfoI T'CCnGG_A
Cuts at: 2301 2301
Size: 6028
PmeI GTTT'AAAC
Cuts at: 3332 3332
Size: 6028
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4097 4129 5610 5642 4097
Size: 32 1481 32 4483
Fragments arranged by size:
4483 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6028
PsiI TTA'TAA
Cuts at: 3598 3598
Size: 6028
PstI C_TGCA'G
Cuts at: 830 2712 2718 2775 3380 830
Size: 1882 6 57 605 3478
Fragments arranged by size:
3478 1882 605 57 6
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6028
PvuII CAG'CTG
Cuts at: 2713 2800 3145 2713
Size: 87 345 5596
Fragments arranged by size:
5596 345 87
SacII CC_GC'GG
Cuts at: 2526 2532 2526
Size: 6 6022
SalI G'TCGA_C
Cuts at: 1936 3370 1936
Size: 1434 4594
SapI GCTCTTCn'nnn_
Cuts at: 2417 2417
Size: 6028
SbfI CC_TGCA'GG
Cuts at: 3380 3380
Size: 6028
ScaI AGT'ACT
Cuts at: 4276 5988 4276
Size: 1712 4316
SexAI A'CCwGG_T
Cuts at: 3312 3312
Size: 6028
SfcI C'TryA_G
Cuts at: 826 1045 2708 2714 2771 2941 3376 4511 5192
Size: 219 1663 6 57 170 435 1135 681
Cuts at: 5192 5383 826
Size: 191 1471
Fragments arranged by size:
1663 1471 1135 681 435 219 191 170
57 6
SfiI GGCCn_nnn'nGGCC
Cuts at: 2525 2525
Size: 6028
SfoI GGC'GCC
Cuts at: 2536 2917 2536
Size: 381 5647
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6028
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6028
SmaI CCC'GGG
Cuts at: 1872 2051 3355 1872
Size: 179 1304 4545
Fragments arranged by size:
4545 1304 179
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2324 2513 4132 5033 5295 820
Size: 197 926 381 189 1619 901 262 1553
Fragments arranged by size:
1619 1553 926 901 381 262 197 189
SnaBI TAC'GTA
Cuts at: 493 3819 493
Size: 3326 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6028
SphI G_CATG'C
Cuts at: 1132 3386 1132
Size: 2254 3774
SspI AAT'ATT
Cuts at: 5 52 3952 5
Size: 47 3900 2081
Fragments arranged by size:
3900 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6028
StyI C'CwwG_G
Cuts at: 513 1065 2004 2484 2538 3467 513
Size: 552 939 480 54 929 3074
Fragments arranged by size:
3074 939 929 552 480 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4055 4072 4225 4410 4855 325
Size: 1255 2475 17 153 185 445 1498
Fragments arranged by size:
2475 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4274 5986 96
Size: 274 80 33 51 1443 2297 1712 138
Fragments arranged by size:
2297 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5988 6020 5988
Size: 32 5996
Tth111I GACn'n_nGTC
Cuts at: 1316 2137 1316
Size: 821 5207
XbaI T'CTAG_A
Cuts at: 3364 3364
Size: 6028
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6028
XmaI C'CCGG_G
Cuts at: 1870 2049 3353 1870
Size: 179 1304 4545
Fragments arranged by size:
4545 1304 179
XmnI GAAnn'nnTTC
Cuts at: 2168 4157 2168
Size: 1989 4039
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3836 276
Size: 53 83 186 936 2302 2468
Fragments arranged by size:
2468 2302 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SacII SalI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI
FseI FspAI NruI NsiI PacI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacI SanDI SrfI SwaI XcmI