Restriction map for
pFN21AB9643
(Circular) (Six-base) MAPSORT of: pFN21AB9643.seq Check: 4040 from: 1 to: 5926
pFN21AB9643 5926 bp
With 182 enzymes: SgfI *
October 20, 2009 10:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3736 278
Size: 53 83 186 936 2200 2468
Fragments arranged by size:
2468 2200 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3269 1937
Size: 1332 4594
Acc65I G'GTAC_C
Cuts at: 3247 3247
Size: 5926
AclI AA'CG_TT
Cuts at: 1563 4053 4426 1563
Size: 2490 373 3063
Fragments arranged by size:
3063 2490 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2172 2249 3049 3989 5373 5641 1632
Size: 540 77 800 940 1384 268 1917
Fragments arranged by size:
1917 1384 940 800 540 268 77
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5729
AflIII A'CryG_T
Cuts at: 1203 2380 4825 5802 1203
Size: 1177 2445 977 1327
Fragments arranged by size:
2445 1327 1177 977
AgeI A'CCGG_T
Cuts at: 4757 4757
Size: 5926
AhdI GACnn_n'nnGTC
Cuts at: 2460 4655 2460
Size: 2195 3731
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5926
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5926
AlwNI CAG_nnn'CTG
Cuts at: 2195 2527 3669 5241 2195
Size: 332 1142 1572 2880
Fragments arranged by size:
2880 1572 1142 332
ApaLI G'TGCA_C
Cuts at: 3983 5139 3983
Size: 1156 4770
ApoI r'AATT_y
Cuts at: 1493 2134 2555 2601 3166 3200 3235 3461 3613
Size: 641 421 46 565 34 35 226 152
Cuts at: 3613 5722 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 641 565 421 226 152 46
35 34
AseI AT'TA_AT
Cuts at: 160 1032 2202 4480 160
Size: 872 1170 2278 1606
Fragments arranged by size:
2278 1606 1170 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5926
AvaI C'yCGr_G
Cuts at: 1870 1943 3251 1870
Size: 73 1308 4545
Fragments arranged by size:
4545 1308 73
BamHI G'GATC_C
Cuts at: 3256 3623 3256
Size: 367 5559
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3247 4702 618
Size: 325 217 627 1460 1455 1842
Fragments arranged by size:
1842 1460 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3245 1391
Size: 1854 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2377 2871 928
Size: 971 478 494 3983
Fragments arranged by size:
3983 971 494 478
BbvCI CC'TCA_GC
Cuts at: 2712 2712
Size: 5926
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4117 4151 1903
Size: 34 2180 34 3678
Fragments arranged by size:
3678 2180 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2482 3818 5028 2482
Size: 1336 1210 3380
Fragments arranged by size:
3380 1336 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5926
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4537 136
Size: 107 122 71 631 605 2865 1525
Fragments arranged by size:
2865 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2023 2262 5921 2023
Size: 239 3659 2028
Fragments arranged by size:
3659 2028 239
BlpI GC'TnA_GC
Cuts at: 3343 3343
Size: 5926
Bme1580I G_kGCm'C
Cuts at: 1790 3987 5143 1790
Size: 2197 1156 2573
Fragments arranged by size:
2573 2197 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5926
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3127 3657 4615
Size: 416 53 83 399 24 1710 530 958
Cuts at: 4615 442
Size: 1753
Fragments arranged by size:
1753 1710 958 530 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5926
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2169 2169
Size: 5926
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2959 4586 1239
Size: 1720 1627 2579
Fragments arranged by size:
2579 1720 1627
Bpu10I CC'TnA_GC
Cuts at: 2412 2712 2412
Size: 300 5626
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2889 4051 4916 5214 2889
Size: 1162 865 298 3601
Fragments arranged by size:
3601 1162 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2140 2447 2612 4589 882
Size: 617 641 307 165 1977 2219
Fragments arranged by size:
2219 1977 641 617 307 165
BsaAI yAC'GTr
Cuts at: 493 1204 3717 493
Size: 711 2513 2702
Fragments arranged by size:
2702 2513 711
BsaBI GATnn'nnATC
Cuts at: 2261 5920 2261
Size: 3659 2267
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2461 3733 4115 4750
Size: 53 83 186 936 928 1272 382 635
Cuts at: 4750 275
Size: 1451
Fragments arranged by size:
1451 1272 936 928 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4359 4757 5031 5178 5589 4359
Size: 398 274 147 411 4696
Fragments arranged by size:
4696 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5701
Fragments arranged by size:
5701 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5926
BseYI C'CCAG_C
Cuts at: 5129 5625 5129
Size: 496 5430
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5353
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3376 4137 5165 1540
Size: 275 186 1375 761 1028 2301
Fragments arranged by size:
2301 1375 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2373 3987 4072 5143 2373
Size: 1614 85 1071 3156
Fragments arranged by size:
3156 1614 1071 85
BsmI GAATG_Cn'
Cuts at: 3437 3530 3437
Size: 93 5833
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2373 3245 3987 4072 5143 1391
Size: 399 583 872 742 85 1071 2174
Fragments arranged by size:
2174 1071 872 742 583 399 85
BspEI T'CCGG_A
Cuts at: 5589 5589
Size: 5926
BspHI T'CATG_A
Cuts at: 3815 5545 3815
Size: 1730 4196
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2203 3281 844
Size: 1359 1078 3489
Fragments arranged by size:
3489 1359 1078
BsrBI CCG'CTC
Cuts at: 1394 1823 3813 1394
Size: 429 1990 3507
Fragments arranged by size:
3507 1990 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2706 4421 4595 65
Size: 1183 1458 1715 174 1396
Fragments arranged by size:
1715 1458 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4570 4757 1700
Size: 110 2760 187 2869
Fragments arranged by size:
2869 2760 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5926
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5926
BssSI C'ACGA_G
Cuts at: 2361 3986 4998 2361
Size: 1625 1012 3289
Fragments arranged by size:
3289 1625 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2100 2758 794
Size: 1306 658 3962
Fragments arranged by size:
3962 1306 658
BstBI TT'CG_AA
Cuts at: 4730 4730
Size: 5926
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5926
BstYI r'GATC_y
Cuts at: 1972 2023 2262 2349 3256 3623 4008 4025 5477
Size: 51 239 87 907 367 385 17 1452
Cuts at: 5477 5563 5575 5921 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 907 385 367 346 239 87
86 51 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2063 513
Size: 552 939 59 4376
Fragments arranged by size:
4376 939 552 59
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5187
Fragments arranged by size:
5187 683 56
BtsI GCAGTG_nn'
Cuts at: 3442 4236 4256 3442
Size: 794 20 5112
Fragments arranged by size:
5112 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5926
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2052 2087 529
Size: 35 1488 35 4368
Fragments arranged by size:
4368 1488 35 35
DraI TTT'AAA
Cuts at: 3230 3230
Size: 5926
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4933 809
Size: 507 3617 1802
Fragments arranged by size:
3617 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3373 4262 5891
Size: 54 1425 50 275 18 1543 889 1629
Cuts at: 5891 8
Size: 43
Fragments arranged by size:
1629 1543 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3373 1537
Size: 275 1561 4090
Fragments arranged by size:
4090 1561 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2444 3856 1353
Size: 1091 1412 3423
Fragments arranged by size:
3423 1412 1091
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4513 4887 5033 387
Size: 1064 138 2924 374 146 1280
Fragments arranged by size:
2924 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2172 2249 2959 3049 3989 4586 5373
Size: 393 540 77 710 90 940 597 787
Cuts at: 5373 5641 1239
Size: 268 1524
Fragments arranged by size:
1524 940 787 710 597 540 393 268
90 77
EcoRI G'AATT_C
Cuts at: 2134 3235 3613 2134
Size: 1101 378 4447
Fragments arranged by size:
4447 1101 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3071 3071
Size: 5926
FspI TGC'GCA
Cuts at: 4432 4432
Size: 5926
HaeII r_GCGC'y
Cuts at: 1678 5073 1678
Size: 3395 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2446 2478 2763 2795 4567
Size: 32 135 32 874 32 285 32 1772
Cuts at: 4567 4599 4641 4673 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1772 874 285 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3270 3516 669
Size: 988 281 1332 246 3079
Fragments arranged by size:
3079 1332 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5926
HpaI GTT'AAC
Cuts at: 3516 3516
Size: 5926
KpnI G_GTAC'C
Cuts at: 3251 3251
Size: 5926
MfeI C'AATT_G
Cuts at: 3525 3525
Size: 5926
MluI A'CGCG_T
Cuts at: 5802 5802
Size: 5926
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5040 5224 1305
Size: 3735 184 2007
Fragments arranged by size:
3735 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4104
Fragments arranged by size:
4104 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2161 3884 4243 4402 518
Size: 648 995 1723 359 159 2042
Fragments arranged by size:
2042 1723 995 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2338 3342 4019 5167 5412 1606
Size: 732 1004 677 1148 245 2120
Fragments arranged by size:
2120 1148 1004 732 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4435
Fragments arranged by size:
4435 939 552
NdeI CA'TA_TG
Cuts at: 387 2647 387
Size: 2260 3666
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5926
NotI GC'GGCC_GC
Cuts at: 3373 3373
Size: 5926
NspI r_CATG'y
Cuts at: 1132 1229 2034 2175 2384 3284 4829 1132
Size: 97 805 141 209 900 1545 2229
Fragments arranged by size:
2229 1545 900 805 209 141 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5926
PciI A'CATG_T
Cuts at: 2380 4825 2380
Size: 2445 3481
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2859 1108
Size: 1751 4175
PmeI GTTT'AAAC
Cuts at: 3230 3230
Size: 5926
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3995 4027 5508 5540 3995
Size: 32 1481 32 4381
Fragments arranged by size:
4381 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5926
PsiI TTA'TAA
Cuts at: 2949 3496 2949
Size: 547 5379
PstI C_TGCA'G
Cuts at: 830 2214 3278 830
Size: 1384 1064 3478
Fragments arranged by size:
3478 1384 1064
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5926
PvuII CAG'CTG
Cuts at: 2338 2338
Size: 5926
SalI G'TCGA_C
Cuts at: 1936 3268 1936
Size: 1332 4594
SbfI CC_TGCA'GG
Cuts at: 2214 3278 2214
Size: 1064 4862
ScaI AGT'ACT
Cuts at: 4174 5886 4174
Size: 1712 4214
SfcI C'TryA_G
Cuts at: 826 1045 2210 2519 3274 4409 5090 5281 826
Size: 219 1165 309 755 1135 681 191 1471
Fragments arranged by size:
1471 1165 1135 755 681 309 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5926
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5926
SmaI CCC'GGG
Cuts at: 1872 3253 1872
Size: 1381 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2904 4030 4931 5193 820
Size: 197 926 961 1126 901 262 1553
Fragments arranged by size:
1553 1126 961 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3717 493
Size: 3224 2702
SpeI A'CTAG_T
Cuts at: 152 2501 2875 152
Size: 2349 374 3203
Fragments arranged by size:
3203 2349 374
SphI G_CATG'C
Cuts at: 1132 3284 1132
Size: 2152 3774
SspI AAT'ATT
Cuts at: 5 52 3850 5
Size: 47 3798 2081
Fragments arranged by size:
3798 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5926
StyI C'CwwG_G
Cuts at: 513 1065 2004 2493 3365 513
Size: 552 939 489 872 3074
Fragments arranged by size:
3074 939 872 552 489
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3953 3970 4123 4308 4753 325
Size: 1255 2373 17 153 185 445 1498
Fragments arranged by size:
2373 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4172 5884 96
Size: 274 80 33 51 1443 2195 1712 138
Fragments arranged by size:
2195 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5886 5918 5886
Size: 32 5894
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5926
XbaI T'CTAG_A
Cuts at: 3262 3262
Size: 5926
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5926
XmaI C'CCGG_G
Cuts at: 1870 3251 1870
Size: 1381 4545
XmnI GAAnn'nnTTC
Cuts at: 4055 4055
Size: 5926
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3734 276
Size: 53 83 186 936 2200 2468
Fragments arranged by size:
2468 2200 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI