Restriction map for
pFN21AB9640
(Circular) (Six-base) MAPSORT of: pFN21AB9640.seq Check: 5011 from: 1 to: 5788
pFN21AB9640 5788 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3598 278
Size: 53 83 186 936 2062 2468
Fragments arranged by size:
2468 2062 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3131 1937
Size: 1194 4594
Acc65I G'GTAC_C
Cuts at: 3109 3109
Size: 5788
AclI AA'CG_TT
Cuts at: 1563 3915 4288 1563
Size: 2352 373 3063
Fragments arranged by size:
3063 2352 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2768 3851 5235 5503 1632
Size: 1136 1083 1384 268 1917
Fragments arranged by size:
1917 1384 1136 1083 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5591
AflIII A'CryG_T
Cuts at: 1203 2302 2400 2679 4687 5664 1203
Size: 1099 98 279 2008 977 1327
Fragments arranged by size:
2008 1327 1099 977 279 98
AgeI A'CCGG_T
Cuts at: 4619 4619
Size: 5788
AhdI GACnn_n'nnGTC
Cuts at: 4517 4517
Size: 5788
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5788
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2292 1184
Size: 1108 4680
AlwNI CAG_nnn'CTG
Cuts at: 2488 3531 5103 2488
Size: 1043 1572 3173
Fragments arranged by size:
3173 1572 1043
ApaI G_GGCC'C
Cuts at: 2201 2201
Size: 5788
ApaLI G'TGCA_C
Cuts at: 3845 5001 3845
Size: 1156 4632
ApoI r'AATT_y
Cuts at: 1493 3097 3323 3475 5584 1493
Size: 1604 226 152 2109 1697
Fragments arranged by size:
2109 1697 1604 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4342 160
Size: 872 3310 1606
Fragments arranged by size:
3310 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5788
AvaI C'yCGr_G
Cuts at: 1870 1943 2162 2252 3113 1870
Size: 73 219 90 861 4545
Fragments arranged by size:
4545 861 219 90 73
BamHI G'GATC_C
Cuts at: 3118 3485 3118
Size: 367 5421
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2359 3109 4564 618
Size: 325 217 627 572 750 1455 1842
Fragments arranged by size:
1842 1455 750 627 572 325 217
BanII G_rGCy'C
Cuts at: 1391 2201 2511 2534 2801 3107 1391
Size: 810 310 23 267 306 4072
Fragments arranged by size:
4072 810 310 306 267 23
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2268 2998 928
Size: 971 369 730 3718
Fragments arranged by size:
3718 971 730 369
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3979 4013 1903
Size: 34 2042 34 3678
Fragments arranged by size:
3678 2042 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3680 4890 3680
Size: 1210 4578
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5788
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2348 2357 4399
Size: 107 122 71 631 605 676 9 2042
Cuts at: 4399 136
Size: 1525
Fragments arranged by size:
2042 1525 676 631 605 122 107 71
9
BglII A'GATC_T
Cuts at: 2925 5783 2925
Size: 2858 2930
BlpI GC'TnA_GC
Cuts at: 2723 3205 2723
Size: 482 5306
Bme1580I G_kGCm'C
Cuts at: 1790 2201 2666 3849 5005 1790
Size: 411 465 1183 1156 2573
Fragments arranged by size:
2573 1183 1156 465 411
BmgBI CAC'GTC
Cuts at: 1318 2245 2379 2647 1318
Size: 927 134 268 4459
Fragments arranged by size:
4459 927 268 134
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2182 2282 2537
Size: 416 53 83 399 24 765 100 255
Cuts at: 2537 3519 4477 442
Size: 982 958 1753
Fragments arranged by size:
1753 982 958 765 416 399 255 100
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5788
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2947 2947
Size: 5788
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2448 2792 2903 2933 4448 1239
Size: 1209 344 111 30 1515 2579
Fragments arranged by size:
2579 1515 1209 344 111 30
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2882 3913 4778 5076 2882
Size: 1031 865 298 3594
Fragments arranged by size:
3594 1031 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4451 882
Size: 617 2952 2219
Fragments arranged by size:
2952 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2305 3579 493
Size: 711 1101 1274 2702
Fragments arranged by size:
2702 1274 1101 711
BsaBI GATnn'nnATC
Cuts at: 5782 5782
Size: 5788
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2567 3595 3977 4612
Size: 53 83 186 936 1034 1028 382 635
Cuts at: 4612 275
Size: 1451
Fragments arranged by size:
1451 1034 1028 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4221 4619 4893 5040 5451 4221
Size: 398 274 147 411 4558
Fragments arranged by size:
4558 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2446 2476 1178
Size: 30 165 30 1043 30 4490
Fragments arranged by size:
4490 1043 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2576 2789 2920 1186
Size: 1390 213 131 4054
Fragments arranged by size:
4054 1390 213 131
BseYI C'CCAG_C
Cuts at: 2144 2659 4991 5487 2144
Size: 515 2332 496 2445
Fragments arranged by size:
2445 2332 515 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2463 2474 2808 1163
Size: 573 727 11 334 4143
Fragments arranged by size:
4143 727 573 334 11
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3238 3999 5027 1540
Size: 275 186 1237 761 1028 2301
Fragments arranged by size:
2301 1237 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2502 3849 3934 5005 2502
Size: 1347 85 1071 3285
Fragments arranged by size:
3285 1347 1071 85
BsmI GAATG_Cn'
Cuts at: 2034 3299 3392 2034
Size: 1265 93 4430
Fragments arranged by size:
4430 1265 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2558 2879 2558
Size: 321 5467
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2201 2502 2511 2534 2666 2801 3107
Size: 399 411 301 9 23 132 135 306
Cuts at: 3107 3849 3934 5005 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 411 399 306 301 135
132 85 23 9
BspEI T'CCGG_A
Cuts at: 5451 5451
Size: 5788
BspHI T'CATG_A
Cuts at: 3677 5407 3677
Size: 1730 4058
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2274 2694 3143 844
Size: 1430 420 449 3489
Fragments arranged by size:
3489 1430 449 420
BsrBI CCG'CTC
Cuts at: 1394 1823 2295 2430 3675 1394
Size: 429 472 135 1245 3507
Fragments arranged by size:
3507 1245 472 429 135
BsrDI GCAATG_nn'
Cuts at: 65 1248 2155 2539 4283 4457 65
Size: 1183 907 384 1744 174 1396
Fragments arranged by size:
1744 1396 1183 907 384 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4432 4619 1700
Size: 110 2622 187 2869
Fragments arranged by size:
2869 2622 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5788
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5788
BssSI C'ACGA_G
Cuts at: 3848 4860 3848
Size: 1012 4776
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2497 794
Size: 1703 4085
BstBI TT'CG_AA
Cuts at: 4592 4592
Size: 5788
BstEII G'GTnAC_C
Cuts at: 1184 2176 1184
Size: 992 4796
BstXI CCAn_nnnn'nTGG
Cuts at: 2230 2230
Size: 5788
BstYI r'GATC_y
Cuts at: 1972 2925 3118 3485 3870 3887 5339 5425 5437
Size: 953 193 367 385 17 1452 86 12
Cuts at: 5437 5783 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 953 385 367 346 193 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2201 513
Size: 552 939 197 4100
Fragments arranged by size:
4100 939 552 197
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5049
Fragments arranged by size:
5049 683 56
BtsI GCAGTG_nn'
Cuts at: 3304 4098 4118 3304
Size: 794 20 4974
Fragments arranged by size:
4974 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5788
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5753
DraI TTT'AAA
Cuts at: 3092 3092
Size: 5788
DraIII CAC_nnn'GTG
Cuts at: 2176 2497 3058 2176
Size: 321 561 4906
Fragments arranged by size:
4906 561 321
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4795 809
Size: 507 3479 1802
Fragments arranged by size:
3479 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2217 2386
Size: 54 1425 50 275 18 177 210 169
Cuts at: 2386 3235 4124 5753 8
Size: 849 889 1629 43
Fragments arranged by size:
1629 1425 889 849 275 210 177 169
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3235 1537
Size: 275 1423 4090
Fragments arranged by size:
4090 1423 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3718 1353
Size: 2365 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2795 3006 4375 4749 4895 387
Size: 1064 138 1206 211 1369 374 146 1280
Fragments arranged by size:
1369 1280 1206 1064 374 211 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2448 2768 2792 2903 2933 3851 4448
Size: 393 816 320 24 111 30 918 597
Cuts at: 4448 5235 5503 1239
Size: 787 268 1524
Fragments arranged by size:
1524 918 816 787 597 393 320 268
111 30 24
EcoO109I rG'GnC_Cy
Cuts at: 2197 2197
Size: 5788
EcoRI G'AATT_C
Cuts at: 3097 3475 3097
Size: 378 5410
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2647 2647
Size: 5788
FspI TGC'GCA
Cuts at: 4294 4294
Size: 5788
HaeII r_GCGC'y
Cuts at: 1678 2660 4935 1678
Size: 982 2275 2531
Fragments arranged by size:
2531 2275 982
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2272 2304 4429 4461 4503
Size: 32 135 32 700 32 2125 32 42
Cuts at: 4503 4535 1373
Size: 32 2626
Fragments arranged by size:
2626 2125 700 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3132 3378 669
Size: 988 281 1194 246 3079
Fragments arranged by size:
3079 1194 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5788
HpaI GTT'AAC
Cuts at: 3378 3378
Size: 5788
KpnI G_GTAC'C
Cuts at: 3113 3113
Size: 5788
MfeI C'AATT_G
Cuts at: 3387 3387
Size: 5788
MluI A'CGCG_T
Cuts at: 5664 5664
Size: 5788
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2565 2580 2934 4902 5086 1305
Size: 1260 15 354 1968 184 2007
Fragments arranged by size:
2007 1968 1260 354 184 15
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 2219 10
Size: 54 1425 343 177 210 3579
Fragments arranged by size:
3579 1425 343 210 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2861 3746 4105 4264 518
Size: 648 1695 885 359 159 2042
Fragments arranged by size:
2042 1695 885 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2015 2468 2722 3204 3881 5029 5274 1606
Size: 409 453 254 482 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 482 453 409 254 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2201 513
Size: 552 939 197 4100
Fragments arranged by size:
4100 939 552 197
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5788
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5788
NotI GC'GGCC_GC
Cuts at: 3235 3235
Size: 5788
NspI r_CATG'y
Cuts at: 1132 1229 2080 2437 3146 4691 1132
Size: 97 851 357 709 1545 2229
Fragments arranged by size:
2229 1545 851 709 357 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5788
PciI A'CATG_T
Cuts at: 4687 4687
Size: 5788
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2343 1108
Size: 1235 4553
PmeI GTTT'AAAC
Cuts at: 3092 3092
Size: 5788
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3857 3889 5370 5402 3857
Size: 32 1481 32 4243
Fragments arranged by size:
4243 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5788
PsiI TTA'TAA
Cuts at: 3358 3358
Size: 5788
PspOMI G'GGCC_C
Cuts at: 2197 2197
Size: 5788
PstI C_TGCA'G
Cuts at: 830 2705 3140 830
Size: 1875 435 3478
Fragments arranged by size:
3478 1875 435
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5788
PvuII CAG'CTG
Cuts at: 2015 2468 2722 2015
Size: 453 254 5081
Fragments arranged by size:
5081 453 254
SalI G'TCGA_C
Cuts at: 1936 3130 1936
Size: 1194 4594
SbfI CC_TGCA'GG
Cuts at: 3140 3140
Size: 5788
ScaI AGT'ACT
Cuts at: 4036 5748 4036
Size: 1712 4076
SfcI C'TryA_G
Cuts at: 826 1045 2029 2701 3136 4271 4952 5143 826
Size: 219 984 672 435 1135 681 191 1471
Fragments arranged by size:
1471 1135 984 681 672 435 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5788
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5788
SmaI CCC'GGG
Cuts at: 1872 2254 3115 1872
Size: 382 861 4545
Fragments arranged by size:
4545 861 382
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2897 3892 4793 5055 820
Size: 197 926 954 995 901 262 1553
Fragments arranged by size:
1553 995 954 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3579 493
Size: 3086 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5788
SphI G_CATG'C
Cuts at: 1132 2080 2437 3146 1132
Size: 948 357 709 3774
Fragments arranged by size:
3774 948 709 357
SrfI GCCC'GGGC
Cuts at: 2254 2254
Size: 5788
SspI AAT'ATT
Cuts at: 5 52 3712 5
Size: 47 3660 2081
Fragments arranged by size:
3660 2081 47
StuI AGG'CCT
Cuts at: 1517 2714 1517
Size: 1197 4591
StyI C'CwwG_G
Cuts at: 513 1065 2004 2201 3227 513
Size: 552 939 197 1026 3074
Fragments arranged by size:
3074 1026 939 552 197
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2164 2264 3815 3832 3985 4170 4615
Size: 1255 584 100 1551 17 153 185 445
Cuts at: 4615 325
Size: 1498
Fragments arranged by size:
1551 1498 1255 584 445 185 153 100
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2403 4034 5746
Size: 274 80 33 51 1443 426 1631 1712
Cuts at: 5746 96
Size: 138
Fragments arranged by size:
1712 1631 1443 426 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2446 2478 5748 5780 2446
Size: 32 3270 32 2454
Fragments arranged by size:
3270 2454 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5788
XbaI T'CTAG_A
Cuts at: 3124 3124
Size: 5788
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2227 2227
Size: 5788
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5788
XmaI C'CCGG_G
Cuts at: 1870 2252 3113 1870
Size: 382 861 4545
Fragments arranged by size:
4545 861 382
XmnI GAAnn'nnTTC
Cuts at: 3917 3917
Size: 5788
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3596 276
Size: 53 83 186 936 2062 2468
Fragments arranged by size:
2468 2062 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SwaI