Restriction map for
pFN21AB9637
(Circular) (Six-base) MAPSORT of: pFN21AB9637.seq Check: 2740 from: 1 to: 5938
pFN21AB9637 5938 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3748 278
Size: 53 83 186 936 2212 2468
Fragments arranged by size:
2468 2212 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3281 1937
Size: 1344 4594
Acc65I G'GTAC_C
Cuts at: 3259 3259
Size: 5938
AclI AA'CG_TT
Cuts at: 1563 4065 4438 1563
Size: 2502 373 3063
Fragments arranged by size:
3063 2502 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2570 4001 5385 5653 1632
Size: 938 1431 1384 268 1917
Fragments arranged by size:
1917 1431 1384 938 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5741
AflIII A'CryG_T
Cuts at: 1203 4837 5814 1203
Size: 3634 977 1327
Fragments arranged by size:
3634 1327 977
AgeI A'CCGG_T
Cuts at: 4769 4769
Size: 5938
AhdI GACnn_n'nnGTC
Cuts at: 4667 4667
Size: 5938
AleI CACnn'nnGTG
Cuts at: 1166 2037 2246 1166
Size: 871 209 4858
Fragments arranged by size:
4858 871 209
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2264 1184
Size: 1080 4858
AlwNI CAG_nnn'CTG
Cuts at: 2479 2680 2954 3681 5253 2479
Size: 201 274 727 1572 3164
Fragments arranged by size:
3164 1572 727 274 201
ApaLI G'TGCA_C
Cuts at: 3995 5151 3995
Size: 1156 4782
ApoI r'AATT_y
Cuts at: 1493 3247 3473 3625 5734 1493
Size: 1754 226 152 2109 1697
Fragments arranged by size:
2109 1754 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4492 160
Size: 872 3460 1606
Fragments arranged by size:
3460 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5938
AvaI C'yCGr_G
Cuts at: 1870 1943 3263 1870
Size: 73 1320 4545
Fragments arranged by size:
4545 1320 73
BamHI G'GATC_C
Cuts at: 3268 3635 3268
Size: 367 5571
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2172 3259 4714 618
Size: 325 217 627 385 1087 1455 1842
Fragments arranged by size:
1842 1455 1087 627 385 325 217
BanII G_rGCy'C
Cuts at: 1391 3257 1391
Size: 1866 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2060 2468 928
Size: 971 161 408 4398
Fragments arranged by size:
4398 971 408 161
BbvCI CC'TCA_GC
Cuts at: 2622 2622
Size: 5938
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4129 4163 1903
Size: 34 2192 34 3678
Fragments arranged by size:
3678 2192 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2725 3830 5040 2725
Size: 1105 1210 3623
Fragments arranged by size:
3623 1210 1105
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5938
BfrBI ATG'CAT
Cuts at: 3230 3230
Size: 5938
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4549 136
Size: 107 122 71 631 605 2877 1525
Fragments arranged by size:
2877 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5933 5933
Size: 5938
BlpI GC'TnA_GC
Cuts at: 3355 3355
Size: 5938
Bme1580I G_kGCm'C
Cuts at: 1790 2227 2419 3140 3999 5155 1790
Size: 437 192 721 859 1156 2573
Fragments arranged by size:
2573 1156 859 721 437 192
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5938
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3669 4627 442
Size: 416 53 83 399 24 2252 958 1753
Fragments arranged by size:
2252 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5938
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2075 2249 4598 1239
Size: 836 174 2349 2579
Fragments arranged by size:
2579 2349 836 174
Bpu10I CC'TnA_GC
Cuts at: 2622 2904 2622
Size: 282 5656
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4063 4928 5226 4063
Size: 865 298 4775
Fragments arranged by size:
4775 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2499 4601 882
Size: 617 1000 2102 2219
Fragments arranged by size:
2219 2102 1000 617
BsaAI yAC'GTr
Cuts at: 493 1204 3729 493
Size: 711 2525 2702
Fragments arranged by size:
2702 2525 711
BsaBI GATnn'nnATC
Cuts at: 5932 5932
Size: 5938
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2365 3745 4127 4762
Size: 53 83 186 936 832 1380 382 635
Cuts at: 4762 275
Size: 1451
Fragments arranged by size:
1451 1380 936 832 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2064 2501 4371 4769 5043 5190 5601 2064
Size: 437 1870 398 274 147 411 2401
Fragments arranged by size:
2401 1870 437 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2002 2032 1178
Size: 30 165 30 599 30 5084
Fragments arranged by size:
5084 599 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2144 2718 1186
Size: 958 574 4406
Fragments arranged by size:
4406 958 574
BseYI C'CCAG_C
Cuts at: 2158 2664 5141 5637 2158
Size: 506 2477 496 2459
Fragments arranged by size:
2477 2459 506 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5365
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3388 4149 5177 1540
Size: 275 186 1387 761 1028 2301
Fragments arranged by size:
2301 1387 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2044 2243 2519 3999 4084 5155 2044
Size: 199 276 1480 85 1071 2827
Fragments arranged by size:
2827 1480 1071 276 199 85
BsmI GAATG_Cn'
Cuts at: 3230 3449 3542 3230
Size: 219 93 5626
Fragments arranged by size:
5626 219 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2044 2227 2243 2419 2519 3140 3257
Size: 399 254 183 16 176 100 621 117
Cuts at: 3257 3999 4084 5155 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 621 399 254 183 176
117 100 85 16
BspEI T'CCGG_A
Cuts at: 5601 5601
Size: 5938
BspHI T'CATG_A
Cuts at: 3827 5557 3827
Size: 1730 4208
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2937 2980 3293 844
Size: 2093 43 313 3489
Fragments arranged by size:
3489 2093 313 43
BsrBI CCG'CTC
Cuts at: 1394 1823 3825 1394
Size: 429 2002 3507
Fragments arranged by size:
3507 2002 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4433 4607 65
Size: 1183 3185 174 1396
Fragments arranged by size:
3185 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4582 4769 1700
Size: 110 2772 187 2869
Fragments arranged by size:
2869 2772 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5938
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5938
BssSI C'ACGA_G
Cuts at: 3998 5010 3998
Size: 1012 4926
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2680 794
Size: 1886 4052
BstBI TT'CG_AA
Cuts at: 4742 4742
Size: 5938
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5938
BstXI CCAn_nnnn'nTGG
Cuts at: 2113 2426 2113
Size: 313 5625
BstYI r'GATC_y
Cuts at: 1972 3268 3635 4020 4037 5489 5575 5587 5933
Size: 1296 367 385 17 1452 86 12 346
Cuts at: 5933 1972
Size: 1977
Fragments arranged by size:
1977 1452 1296 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3132 513
Size: 552 939 1128 3319
Fragments arranged by size:
3319 1128 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5199
Fragments arranged by size:
5199 683 56
BtsI GCAGTG_nn'
Cuts at: 2203 3454 4248 4268 2203
Size: 1251 794 20 3873
Fragments arranged by size:
3873 1251 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5938
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5903
DraI TTT'AAA
Cuts at: 3242 3242
Size: 5938
DraIII CAC_nnn'GTG
Cuts at: 2374 2374
Size: 5938
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4945 809
Size: 507 3629 1802
Fragments arranged by size:
3629 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2190 3385 4274
Size: 54 1425 50 275 18 360 1195 889
Cuts at: 4274 5903 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1195 889 360 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3385 1537
Size: 275 1573 4090
Fragments arranged by size:
4090 1573 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2274 3868 1353
Size: 921 1594 3423
Fragments arranged by size:
3423 1594 921
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2721 4525 4899 5045 387
Size: 1064 138 1132 1804 374 146 1280
Fragments arranged by size:
1804 1280 1132 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2075 2249 2570 4001 4598 5385 5653
Size: 393 443 174 321 1431 597 787 268
Cuts at: 5653 1239
Size: 1524
Fragments arranged by size:
1524 1431 787 597 443 393 321 268
174
EcoNI CCTnn'n_nnAGG
Cuts at: 2933 2933
Size: 5938
EcoO109I rG'GnC_Cy
Cuts at: 2203 3065 2203
Size: 862 5076
EcoRI G'AATT_C
Cuts at: 3247 3625 3247
Size: 378 5560
FspI TGC'GCA
Cuts at: 4444 4444
Size: 5938
HaeII r_GCGC'y
Cuts at: 1678 5085 1678
Size: 3407 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2852 2884 4579 4611 4653
Size: 32 135 32 1280 32 1695 32 42
Cuts at: 4653 4685 1373
Size: 32 2626
Fragments arranged by size:
2626 1695 1280 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3282 3528 669
Size: 988 281 1344 246 3079
Fragments arranged by size:
3079 1344 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5938
HpaI GTT'AAC
Cuts at: 3528 3528
Size: 5938
KpnI G_GTAC'C
Cuts at: 3263 3263
Size: 5938
MfeI C'AATT_G
Cuts at: 3537 3537
Size: 5938
MluI A'CGCG_T
Cuts at: 5814 5814
Size: 5938
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2569 5052 5236 1305
Size: 1264 2483 184 2007
Fragments arranged by size:
2483 2007 1264 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4116
Fragments arranged by size:
4116 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2037 2246 3896 4255 4414 518
Size: 648 871 209 1650 359 159 2042
Fragments arranged by size:
2042 1650 871 648 359 209 159
MspA1I CmG'CkG
Cuts at: 1606 2621 2680 3013 3191 3354 4031 5179 5424
Size: 1015 59 333 178 163 677 1148 245
Cuts at: 5424 1606
Size: 2120
Fragments arranged by size:
2120 1148 1015 677 333 245 178 163
59
NcoI C'CATG_G
Cuts at: 513 1065 2004 3132 513
Size: 552 939 1128 3319
Fragments arranged by size:
3319 1128 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5938
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5938
NotI GC'GGCC_GC
Cuts at: 3385 3385
Size: 5938
NsiI A_TGCA'T
Cuts at: 3232 3232
Size: 5938
NspI r_CATG'y
Cuts at: 1132 1229 3296 4841 1132
Size: 97 2067 1545 2229
Fragments arranged by size:
2229 2067 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3061 1348
Size: 1713 4225
PciI A'CATG_T
Cuts at: 4837 4837
Size: 5938
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2374 1108
Size: 1266 4672
PmeI GTTT'AAAC
Cuts at: 3242 3242
Size: 5938
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2042 2074 4007 4039 5520 5552 2042
Size: 32 1933 32 1481 32 2428
Fragments arranged by size:
2428 1933 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2203 2203
Size: 5938
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5938
PsiI TTA'TAA
Cuts at: 3508 3508
Size: 5938
PstI C_TGCA'G
Cuts at: 830 2950 3290 830
Size: 2120 340 3478
Fragments arranged by size:
3478 2120 340
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5938
PvuII CAG'CTG
Cuts at: 2621 3013 3191 2621
Size: 392 178 5368
Fragments arranged by size:
5368 392 178
SalI G'TCGA_C
Cuts at: 1936 3280 1936
Size: 1344 4594
SbfI CC_TGCA'GG
Cuts at: 3290 3290
Size: 5938
ScaI AGT'ACT
Cuts at: 4186 5898 4186
Size: 1712 4226
SexAI A'CCwGG_T
Cuts at: 2370 2370
Size: 5938
SfcI C'TryA_G
Cuts at: 826 1045 2946 3286 4421 5102 5293 826
Size: 219 1901 340 1135 681 191 1471
Fragments arranged by size:
1901 1471 1135 681 340 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5938
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5938
SmaI CCC'GGG
Cuts at: 1872 3265 1872
Size: 1393 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4042 4943 5205 820
Size: 197 926 2099 901 262 1553
Fragments arranged by size:
2099 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3729 493
Size: 3236 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5938
SphI G_CATG'C
Cuts at: 1132 3296 1132
Size: 2164 3774
SspI AAT'ATT
Cuts at: 5 52 3862 5
Size: 47 3810 2081
Fragments arranged by size:
3810 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5938
StyI C'CwwG_G
Cuts at: 513 1065 2004 2301 2934 3132 3377 513
Size: 552 939 297 633 198 245 3074
Fragments arranged by size:
3074 939 633 552 297 245 198
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2112 3965 3982 4135 4320 4765 325
Size: 1255 532 1853 17 153 185 445 1498
Fragments arranged by size:
1853 1498 1255 532 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2145 2754 4184
Size: 274 80 33 51 1443 168 609 1430
Cuts at: 4184 5896 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1430 609 274 168 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2387 2419 2629 2661 5898 5930 2387
Size: 32 210 32 3237 32 2395
Fragments arranged by size:
3237 2395 210 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5938
XbaI T'CTAG_A
Cuts at: 3274 3274
Size: 5938
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3038 3038
Size: 5938
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5938
XmaI C'CCGG_G
Cuts at: 1870 3263 1870
Size: 1393 4545
XmnI GAAnn'nnTTC
Cuts at: 2057 4067 2057
Size: 2010 3928
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3746 276
Size: 53 83 186 936 2212 2468
Fragments arranged by size:
2468 2212 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SapI SfiI SfoI SrfI SwaI