Restriction map for
pFN21AB9636
(Circular) (Six-base) MAPSORT of: pFN21AB9636.seq Check: 8243 from: 1 to: 5521
pFN21AB9636 5521 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3331 278
Size: 53 83 186 936 1795 2468
Fragments arranged by size:
2468 1795 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2864 1937
Size: 927 4594
Acc65I G'GTAC_C
Cuts at: 2842 2842
Size: 5521
AclI AA'CG_TT
Cuts at: 1563 3648 4021 1563
Size: 2085 373 3063
Fragments arranged by size:
3063 2085 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2282 3584 4968 5236 1632
Size: 650 1302 1384 268 1917
Fragments arranged by size:
1917 1384 1302 650 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5324
AflIII A'CryG_T
Cuts at: 1203 4420 5397 1203
Size: 3217 977 1327
Fragments arranged by size:
3217 1327 977
AgeI A'CCGG_T
Cuts at: 4352 4352
Size: 5521
AhdI GACnn_n'nnGTC
Cuts at: 4250 4250
Size: 5521
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5521
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2306 1184
Size: 1122 4399
AlwNI CAG_nnn'CTG
Cuts at: 3264 4836 3264
Size: 1572 3949
ApaLI G'TGCA_C
Cuts at: 2537 2546 3578 4734 2537
Size: 9 1032 1156 3324
Fragments arranged by size:
3324 1156 1032 9
ApoI r'AATT_y
Cuts at: 1493 2008 2830 3056 3208 5317 1493
Size: 515 822 226 152 2109 1697
Fragments arranged by size:
2109 1697 822 515 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4075 160
Size: 872 3043 1606
Fragments arranged by size:
3043 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5521
AvaI C'yCGr_G
Cuts at: 1870 1943 2159 2846 1870
Size: 73 216 687 4545
Fragments arranged by size:
4545 687 216 73
BamHI G'GATC_C
Cuts at: 2851 3218 2851
Size: 367 5154
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2183 2624 2656 2842 4297
Size: 325 217 627 396 441 32 186 1455
Cuts at: 4297 618
Size: 1842
Fragments arranged by size:
1842 1455 627 441 396 325 217 186
32
BanII G_rGCy'C
Cuts at: 1391 2024 2840 1391
Size: 633 816 4072
Fragments arranged by size:
4072 816 633
BbeI G_GCGC'C
Cuts at: 2187 2660 2187
Size: 473 5048
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4550
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3712 3746 1903
Size: 34 1775 34 3678
Fragments arranged by size:
3678 1775 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3413 4623 3413
Size: 1210 4311
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5521
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2783 4132 136
Size: 107 122 71 631 605 1111 1349 1525
Fragments arranged by size:
1525 1349 1111 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2454 5516 2454
Size: 3062 2459
BlpI GC'TnA_GC
Cuts at: 2938 2938
Size: 5521
Bme1580I G_kGCm'C
Cuts at: 1790 2541 2550 3582 4738 1790
Size: 751 9 1032 1156 2573
Fragments arranged by size:
2573 1156 1032 751 9
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5521
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2713 3252 4210
Size: 416 53 83 399 24 1296 539 958
Cuts at: 4210 442
Size: 1753
Fragments arranged by size:
1753 1296 958 539 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5521
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2014 2447 4181 1239
Size: 775 433 1734 2579
Fragments arranged by size:
2579 1734 775 433
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2636 2801 3646 4511 4809 2636
Size: 165 845 865 298 3348
Fragments arranged by size:
3348 865 845 298 165
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2128 4184 882
Size: 617 629 2056 2219
Fragments arranged by size:
2219 2056 629 617
BsaAI yAC'GTr
Cuts at: 493 1204 3312 493
Size: 711 2108 2702
Fragments arranged by size:
2702 2108 711
BsaBI GATnn'nnATC
Cuts at: 5515 5515
Size: 5521
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2184 2657 3328 3710
Size: 53 83 186 936 651 473 671 382
Cuts at: 3710 4345 275
Size: 635 1451
Fragments arranged by size:
1451 936 671 651 635 473 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2047 3954 4352 4626 4773 5184 2047
Size: 1907 398 274 147 411 2384
Fragments arranged by size:
2384 1907 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5296
Fragments arranged by size:
5296 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2444 1186
Size: 1258 4263
BseYI C'CCAG_C
Cuts at: 2683 4724 5220 2683
Size: 2041 496 2984
Fragments arranged by size:
2984 2041 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2710 1163
Size: 573 974 3974
Fragments arranged by size:
3974 974 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2508 2799 2971 3732 4760 1540
Size: 275 186 507 291 172 761 1028 2301
Fragments arranged by size:
2301 1028 761 507 291 275 186 172
BsiHKAI G_wGCw'C
Cuts at: 2024 2541 2550 3582 3667 4738 2024
Size: 517 9 1032 85 1071 2807
Fragments arranged by size:
2807 1071 1032 517 85 9
BsmI GAATG_Cn'
Cuts at: 3032 3125 3032
Size: 93 5428
BsmBI CGTCTCn'nnnn_
Cuts at: 2117 2117
Size: 5521
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2024 2541 2550 2840 3582 3667 4738
Size: 399 234 517 9 290 742 85 1071
Cuts at: 4738 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 517 399 290 234 85
9
BspEI T'CCGG_A
Cuts at: 2047 5184 2047
Size: 3137 2384
BspHI T'CATG_A
Cuts at: 3410 5140 3410
Size: 1730 3791
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2876 844
Size: 2032 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2765 3408 1394
Size: 429 942 643 3507
Fragments arranged by size:
3507 942 643 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4016 4190 65
Size: 1183 2768 174 1396
Fragments arranged by size:
2768 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2507 4165 4352 1700
Size: 110 697 1658 187 2869
Fragments arranged by size:
2869 1658 697 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5521
BssHII G'CGCG_C
Cuts at: 1925 2565 1925
Size: 640 4881
BssSI C'ACGA_G
Cuts at: 3581 4593 3581
Size: 1012 4509
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2155 2545 2733 794
Size: 1361 390 188 3582
Fragments arranged by size:
3582 1361 390 188
BstBI TT'CG_AA
Cuts at: 4325 4325
Size: 5521
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5521
BstXI CCAn_nnnn'nTGG
Cuts at: 2425 2425
Size: 5521
BstYI r'GATC_y
Cuts at: 1972 2454 2851 3218 3603 3620 5072 5158 5170
Size: 482 397 367 385 17 1452 86 12
Cuts at: 5170 5516 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 482 397 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2069 2069
Size: 5521
BtgI C'CryG_G
Cuts at: 513 1065 2193 2310 2493 513
Size: 552 1128 117 183 3541
Fragments arranged by size:
3541 1128 552 183 117
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4782
Fragments arranged by size:
4782 683 56
BtsI GCAGTG_nn'
Cuts at: 2482 3037 3831 3851 2482
Size: 555 794 20 4152
Fragments arranged by size:
4152 794 555 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5521
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5486
DraI TTT'AAA
Cuts at: 2825 2825
Size: 5521
DraIII CAC_nnn'GTG
Cuts at: 2545 2545
Size: 5521
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4528 809
Size: 507 3212 1802
Fragments arranged by size:
3212 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2505 2509 2526
Size: 54 1425 50 275 18 675 4 17
Cuts at: 2526 2796 2968 3857 5486 8
Size: 270 172 889 1629 43
Fragments arranged by size:
1629 1425 889 675 275 270 172 54
50 43 18 17 4
EagI C'GGCC_G
Cuts at: 1537 1812 2505 2796 2968 1537
Size: 275 693 291 172 4090
Fragments arranged by size:
4090 693 291 275 172
EarI CTCTTCn'nnn_
Cuts at: 1353 2432 3451 1353
Size: 1079 1019 3423
Fragments arranged by size:
3423 1079 1019
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2590 4108 4482 4628 387
Size: 1064 138 1001 1518 374 146 1280
Fragments arranged by size:
1518 1280 1064 1001 374 146 138
EcoICRI GAG'CTC
Cuts at: 2022 2022
Size: 5521
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2014 2282 2447 3584 4181 4968 5236
Size: 393 382 268 165 1137 597 787 268
Cuts at: 5236 1239
Size: 1524
Fragments arranged by size:
1524 1137 787 597 393 382 268 268
165
EcoO109I rG'GnC_Cy
Cuts at: 2242 2242
Size: 5521
EcoRI G'AATT_C
Cuts at: 2008 2830 3208 2008
Size: 822 378 4321
Fragments arranged by size:
4321 822 378
FseI GG_CCGG'CC
Cuts at: 2511 2511
Size: 5521
FspI TGC'GCA
Cuts at: 2360 4027 2360
Size: 1667 3854
HaeII r_GCGC'y
Cuts at: 1678 2187 2660 4668 1678
Size: 509 473 2008 2531
Fragments arranged by size:
2531 2008 509 473
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2006 2038 4162 4194 4236
Size: 32 135 32 434 32 2124 32 42
Cuts at: 4236 4268 1373
Size: 32 2626
Fragments arranged by size:
2626 2124 434 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2865 3111 669
Size: 988 281 927 246 3079
Fragments arranged by size:
3079 988 927 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5521
HpaI GTT'AAC
Cuts at: 3111 3111
Size: 5521
KasI G'GCGC_C
Cuts at: 2183 2656 2183
Size: 473 5048
KpnI G_GTAC'C
Cuts at: 2846 2846
Size: 5521
MfeI C'AATT_G
Cuts at: 3120 3120
Size: 5521
MluI A'CGCG_T
Cuts at: 5397 5397
Size: 5521
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2214 4635 4819 1305
Size: 909 2421 184 2007
Fragments arranged by size:
2421 2007 909 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3699
Fragments arranged by size:
3699 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2408 3479 3838 3997 518
Size: 648 1242 1071 359 159 2042
Fragments arranged by size:
2042 1242 1071 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2195 2230 2596 2683 2691 2937 3614 4762
Size: 589 35 366 87 8 246 677 1148
Cuts at: 4762 5007 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 589 366 246 245 87
35 8
NaeI GCC'GGC
Cuts at: 2509 2509
Size: 5521
NarI GG'CG_CC
Cuts at: 2184 2657 2184
Size: 473 5048
NcoI C'CATG_G
Cuts at: 513 1065 2310 513
Size: 552 1245 3724
Fragments arranged by size:
3724 1245 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5521
NgoMIV G'CCGG_C
Cuts at: 2507 2507
Size: 5521
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5521
NotI GC'GGCC_GC
Cuts at: 2968 2968
Size: 5521
NspI r_CATG'y
Cuts at: 1132 1229 2879 4424 1132
Size: 97 1650 1545 2229
Fragments arranged by size:
2229 1650 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5521
PciI A'CATG_T
Cuts at: 4420 4420
Size: 5521
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5521
PmeI GTTT'AAAC
Cuts at: 2825 2825
Size: 5521
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3590 3622 5103 5135 3590
Size: 32 1481 32 3976
Fragments arranged by size:
3976 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2242 2242
Size: 5521
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5521
PsiI TTA'TAA
Cuts at: 3091 3091
Size: 5521
PstI C_TGCA'G
Cuts at: 830 2682 2873 830
Size: 1852 191 3478
Fragments arranged by size:
3478 1852 191
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5521
PvuII CAG'CTG
Cuts at: 2230 2683 2230
Size: 453 5068
SacI G_AGCT'C
Cuts at: 2024 2024
Size: 5521
SacII CC_GC'GG
Cuts at: 2196 2196
Size: 5521
SalI G'TCGA_C
Cuts at: 1936 2863 1936
Size: 927 4594
SbfI CC_TGCA'GG
Cuts at: 2873 2873
Size: 5521
ScaI AGT'ACT
Cuts at: 3769 5481 3769
Size: 1712 3809
SfcI C'TryA_G
Cuts at: 826 1045 2246 2678 2869 4004 4685 4876 826
Size: 219 1201 432 191 1135 681 191 1471
Fragments arranged by size:
1471 1201 1135 681 432 219 191 191
SfoI GGC'GCC
Cuts at: 2185 2658 2185
Size: 473 5048
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5521
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5521
SmaI CCC'GGG
Cuts at: 1872 2161 2848 1872
Size: 289 687 4545
Fragments arranged by size:
4545 687 289
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2651 2816 3625 4526 4788 820
Size: 197 926 708 165 809 901 262 1553
Fragments arranged by size:
1553 926 901 809 708 262 197 165
SnaBI TAC'GTA
Cuts at: 493 3312 493
Size: 2819 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5521
SphI G_CATG'C
Cuts at: 1132 2879 1132
Size: 1747 3774
SspI AAT'ATT
Cuts at: 5 52 3445 5
Size: 47 3393 2081
Fragments arranged by size:
3393 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5521
StyI C'CwwG_G
Cuts at: 513 1065 2187 2310 2805 2960 513
Size: 552 1122 123 495 155 3074
Fragments arranged by size:
3074 1122 552 495 155 123
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2110 3548 3565 3718 3903 4348 325
Size: 1255 530 1438 17 153 185 445 1498
Fragments arranged by size:
1498 1438 1255 530 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2211 3767 5479
Size: 274 80 33 51 1443 234 1556 1712
Cuts at: 5479 96
Size: 138
Fragments arranged by size:
1712 1556 1443 274 234 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5481 5513 5481
Size: 32 5489
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5521
XbaI T'CTAG_A
Cuts at: 2857 2857
Size: 5521
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2705 2779 2705
Size: 74 5447
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5521
XmaI C'CCGG_G
Cuts at: 1870 2159 2846 1870
Size: 289 687 4545
Fragments arranged by size:
4545 687 289
XmnI GAAnn'nnTTC
Cuts at: 3650 3650
Size: 5521
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3329 276
Size: 53 83 186 936 1795 2468
Fragments arranged by size:
2468 1795 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FseI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SacII SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsiWI BstZ17I EcoNI EcoRV FalI
FspAI NruI NsiI PacI PfoI PmlI PspOMI PspXI
PsrI RsrII SanDI SapI SexAI SfiI SrfI SwaI