Restriction map for
pFN21AB9633
(Circular) (Six-base) MAPSORT of: pFN21AB9633.seq Check: 836 from: 1 to: 6091
pFN21AB9633 6091 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3901 278
Size: 53 83 186 936 2365 2468
Fragments arranged by size:
2468 2365 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2287 3434 1937
Size: 350 1147 4594
Fragments arranged by size:
4594 1147 350
Acc65I G'GTAC_C
Cuts at: 3412 3412
Size: 6091
AclI AA'CG_TT
Cuts at: 1563 4218 4591 1563
Size: 2655 373 3063
Fragments arranged by size:
3063 2655 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3363 4154 5538 5806 1632
Size: 1731 791 1384 268 1917
Fragments arranged by size:
1917 1731 1384 791 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5894
AflIII A'CryG_T
Cuts at: 1203 4990 5967 1203
Size: 3787 977 1327
Fragments arranged by size:
3787 1327 977
AgeI A'CCGG_T
Cuts at: 4922 4922
Size: 6091
AhdI GACnn_n'nnGTC
Cuts at: 4820 4820
Size: 6091
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6091
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2946 1184
Size: 1762 4329
AlwNI CAG_nnn'CTG
Cuts at: 3386 3834 5406 3386
Size: 448 1572 4071
Fragments arranged by size:
4071 1572 448
ApaI G_GGCC'C
Cuts at: 2897 2897
Size: 6091
ApaLI G'TGCA_C
Cuts at: 4148 5304 4148
Size: 1156 4935
ApoI r'AATT_y
Cuts at: 1493 2198 2752 2959 3035 3400 3626 3778 5887
Size: 705 554 207 76 365 226 152 2109
Cuts at: 5887 1493
Size: 1697
Fragments arranged by size:
2109 1697 705 554 365 226 207 152
76
AseI AT'TA_AT
Cuts at: 160 1032 4645 160
Size: 872 3613 1606
Fragments arranged by size:
3613 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6091
AvaI C'yCGr_G
Cuts at: 1870 1943 2818 3416 1870
Size: 73 875 598 4545
Fragments arranged by size:
4545 875 598 73
BamHI G'GATC_C
Cuts at: 3421 3788 3421
Size: 367 5724
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3412 4867 618
Size: 325 217 627 1625 1455 1842
Fragments arranged by size:
1842 1625 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2612 2897 3410 1391
Size: 1221 285 513 4072
Fragments arranged by size:
4072 1221 513 285
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2121 2877 928
Size: 971 222 756 4142
Fragments arranged by size:
4142 971 756 222
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4282 4316 1903
Size: 34 50 34 2261 34 3678
Fragments arranged by size:
3678 2261 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3983 5193 3983
Size: 1210 4881
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6091
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4702 136
Size: 107 122 71 631 605 3030 1525
Fragments arranged by size:
3030 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2270 6086 2270
Size: 3816 2275
BlpI GC'TnA_GC
Cuts at: 2355 3508 2355
Size: 1153 4938
Bme1580I G_kGCm'C
Cuts at: 1790 2897 3115 4152 5308 1790
Size: 1107 218 1037 1156 2573
Fragments arranged by size:
2573 1156 1107 1037 218
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6091
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2336 3822 4780
Size: 416 53 83 399 24 919 1486 958
Cuts at: 4780 442
Size: 1753
Fragments arranged by size:
1753 1486 958 919 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6091
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3355 3355
Size: 6091
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2791 2892 3264 4751 1239
Size: 1552 101 372 1487 2579
Fragments arranged by size:
2579 1552 1487 372 101
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2394 2903 3329 4216 5081 5379 2394
Size: 509 426 887 865 298 3106
Fragments arranged by size:
3106 887 865 509 426 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3183 4754 882
Size: 617 1684 1571 2219
Fragments arranged by size:
2219 1684 1571 617
BsaAI yAC'GTr
Cuts at: 493 1204 3882 493
Size: 711 2678 2702
Fragments arranged by size:
2702 2678 711
BsaBI GATnn'nnATC
Cuts at: 6085 6085
Size: 6091
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2045 3898 4280 4915
Size: 53 83 186 936 512 1853 382 635
Cuts at: 4915 275
Size: 1451
Fragments arranged by size:
1853 1451 936 635 512 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2985 4524 4922 5196 5343 5754 2985
Size: 1539 398 274 147 411 3322
Fragments arranged by size:
3322 1539 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2030 2060 2789 2819 2967
Size: 30 165 30 627 30 729 30 148
Cuts at: 2967 2997 1178
Size: 30 4272
Fragments arranged by size:
4272 729 627 165 148 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2147 1186
Size: 961 5130
BseYI C'CCAG_C
Cuts at: 5294 5790 5294
Size: 496 5595
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2636 1163
Size: 573 900 4618
Fragments arranged by size:
4618 900 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3541 4302 5330 1540
Size: 275 186 1540 761 1028 2301
Fragments arranged by size:
2301 1540 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2502 2612 3195 4152 4237 5308 2502
Size: 110 583 957 85 1071 3285
Fragments arranged by size:
3285 1071 957 583 110 85
BsmI GAATG_Cn'
Cuts at: 3602 3695 3602
Size: 93 5998
BsmBI CGTCTCn'nnnn_
Cuts at: 2052 2052
Size: 6091
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2502 2612 2897 3115 3195 3410 4152
Size: 399 712 110 285 218 80 215 742
Cuts at: 4152 4237 5308 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 712 399 285 218 215
110 85 80
BspEI T'CCGG_A
Cuts at: 2985 5754 2985
Size: 2769 3322
BspHI T'CATG_A
Cuts at: 3980 5710 3980
Size: 1730 4361
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3446 844
Size: 2602 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3978 1394
Size: 429 2155 3507
Fragments arranged by size:
3507 2155 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2071 4586 4760 65
Size: 1183 823 2515 174 1396
Fragments arranged by size:
2515 1396 1183 823 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4735 4922 1700
Size: 110 2925 187 2869
Fragments arranged by size:
2925 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6091
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6091
BssSI C'ACGA_G
Cuts at: 2274 4151 5163 2274
Size: 1877 1012 3202
Fragments arranged by size:
3202 1877 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6091
BstBI TT'CG_AA
Cuts at: 3093 4895 3093
Size: 1802 4289
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6091
BstYI r'GATC_y
Cuts at: 1972 2270 3421 3788 4173 4190 5642 5728 5740
Size: 298 1151 367 385 17 1452 86 12
Cuts at: 5740 6086 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1151 385 367 346 298 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5539
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5352
Fragments arranged by size:
5352 683 56
BtsI GCAGTG_nn'
Cuts at: 3607 4401 4421 3607
Size: 794 20 5277
Fragments arranged by size:
5277 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6091
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6056
DraI TTT'AAA
Cuts at: 2349 3395 2349
Size: 1046 5045
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5098 809
Size: 507 3782 1802
Fragments arranged by size:
3782 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3538 4427 6056
Size: 54 1425 50 275 18 1708 889 1629
Cuts at: 6056 8
Size: 43
Fragments arranged by size:
1708 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3538 1537
Size: 275 1726 4090
Fragments arranged by size:
4090 1726 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4021 1353
Size: 2668 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2260 4678 5052 5198 387
Size: 1064 138 671 2418 374 146 1280
Fragments arranged by size:
2418 1280 1064 671 374 146 138
EcoICRI GAG'CTC
Cuts at: 2610 2610
Size: 6091
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2791 2892 3264 3363 4154 4751 5538
Size: 393 1159 101 372 99 791 597 787
Cuts at: 5538 5806 1239
Size: 268 1524
Fragments arranged by size:
1524 1159 791 787 597 393 372 268
101 99
EcoO109I rG'GnC_Cy
Cuts at: 2884 2893 2894 2884
Size: 9 1 6081
Fragments arranged by size:
6081 9 1
EcoRI G'AATT_C
Cuts at: 2198 2752 2959 3400 3778 2198
Size: 554 207 441 378 4511
Fragments arranged by size:
4511 554 441 378 207
FspI TGC'GCA
Cuts at: 4597 4597
Size: 6091
HaeII r_GCGC'y
Cuts at: 1678 5238 1678
Size: 3560 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2030 2062 2699 2731 3062
Size: 32 135 32 458 32 637 32 331
Cuts at: 3062 3094 4732 4764 4806 4838 1373
Size: 32 1638 32 42 32 2626
Fragments arranged by size:
2626 1638 637 458 331 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2288 2797 3435 3681 669
Size: 988 281 350 509 638 246 3079
Fragments arranged by size:
3079 988 638 509 350 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6091
HpaI GTT'AAC
Cuts at: 3681 3681
Size: 6091
KpnI G_GTAC'C
Cuts at: 3416 3416
Size: 6091
MfeI C'AATT_G
Cuts at: 3690 3690
Size: 6091
MluI A'CGCG_T
Cuts at: 5967 5967
Size: 6091
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2165 2808 5205 5389 1305
Size: 860 643 2397 184 2007
Fragments arranged by size:
2397 2007 860 643 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4269
Fragments arranged by size:
4269 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2928 4049 4408 4567 518
Size: 648 1762 1121 359 159 2042
Fragments arranged by size:
2042 1762 1121 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2011 2441 3507 4184 5332 5577 1606
Size: 405 430 1066 677 1148 245 2120
Fragments arranged by size:
2120 1148 1066 677 430 405 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5539
NdeI CA'TA_TG
Cuts at: 387 2404 387
Size: 2017 4074
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6091
NotI GC'GGCC_GC
Cuts at: 3538 3538
Size: 6091
NspI r_CATG'y
Cuts at: 1132 1229 2184 2927 2933 3449 4994 1132
Size: 97 955 743 6 516 1545 2229
Fragments arranged by size:
2229 1545 955 743 516 97 6
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6091
PciI A'CATG_T
Cuts at: 4990 4990
Size: 6091
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6091
PmeI GTTT'AAAC
Cuts at: 3395 3395
Size: 6091
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4160 4192 5673 5705 4160
Size: 32 1481 32 4546
Fragments arranged by size:
4546 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2884 2884
Size: 6091
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6091
PsiI TTA'TAA
Cuts at: 3661 3661
Size: 6091
PspOMI G'GGCC_C
Cuts at: 2893 2893
Size: 6091
PstI C_TGCA'G
Cuts at: 830 2630 3331 3443 830
Size: 1800 701 112 3478
Fragments arranged by size:
3478 1800 701 112
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6091
PvuII CAG'CTG
Cuts at: 2011 2441 2011
Size: 430 5661
SacI G_AGCT'C
Cuts at: 2612 2612
Size: 6091
SalI G'TCGA_C
Cuts at: 1936 2286 3433 1936
Size: 350 1147 4594
Fragments arranged by size:
4594 1147 350
SbfI CC_TGCA'GG
Cuts at: 3443 3443
Size: 6091
ScaI AGT'ACT
Cuts at: 4339 6051 4339
Size: 1712 4379
SexAI A'CCwGG_T
Cuts at: 2298 2298
Size: 6091
SfcI C'TryA_G
Cuts at: 826 1045 2436 2626 3327 3439 4574 5255 5446
Size: 219 1391 190 701 112 1135 681 191
Cuts at: 5446 826
Size: 1471
Fragments arranged by size:
1471 1391 1135 701 681 219 191 190
112
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6091
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6091
SmaI CCC'GGG
Cuts at: 1872 3418 1872
Size: 1546 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2409 2918 3344 4195 5096 5358
Size: 197 926 466 509 426 851 901 262
Cuts at: 5358 820
Size: 1553
Fragments arranged by size:
1553 926 901 851 509 466 426 262
197
SnaBI TAC'GTA
Cuts at: 493 3882 493
Size: 3389 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6091
SphI G_CATG'C
Cuts at: 1132 2933 3449 1132
Size: 1801 516 3774
Fragments arranged by size:
3774 1801 516
SspI AAT'ATT
Cuts at: 5 52 4015 5
Size: 47 3963 2081
Fragments arranged by size:
3963 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6091
StyI C'CwwG_G
Cuts at: 513 1065 2889 3530 513
Size: 552 1824 641 3074
Fragments arranged by size:
3074 1824 641 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2724 4118 4135 4288 4473 4918 325
Size: 1255 1144 1394 17 153 185 445 1498
Fragments arranged by size:
1498 1394 1255 1144 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2445 4337 6049
Size: 274 80 33 51 1443 468 1892 1712
Cuts at: 6049 96
Size: 138
Fragments arranged by size:
1892 1712 1443 468 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2967 2999 6051 6083 2967
Size: 32 3052 32 2975
Fragments arranged by size:
3052 2975 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6091
XbaI T'CTAG_A
Cuts at: 3427 3427
Size: 6091
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2065 2065
Size: 6091
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6091
XmaI C'CCGG_G
Cuts at: 1870 3416 1870
Size: 1546 4545
XmnI GAAnn'nnTTC
Cuts at: 2202 2756 3233 4220 2202
Size: 554 477 987 4073
Fragments arranged by size:
4073 987 554 477
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3899 276
Size: 53 83 186 936 2365 2468
Fragments arranged by size:
2468 2365 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII
SacII SanDI SapI SfiI SfoI SrfI SwaI