Restriction map for
pFN21AB9628
(Circular) (Six-base) MAPSORT of: pFN21AB9628.seq Check: 9187 from: 1 to: 5698
pFN21AB9628 5698 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3508 278
Size: 53 83 186 936 1972 2468
Fragments arranged by size:
2468 1972 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3041 1937
Size: 1104 4594
Acc65I G'GTAC_C
Cuts at: 2906 3019 2906
Size: 113 5585
AclI AA'CG_TT
Cuts at: 1563 3825 4198 1563
Size: 2262 373 3063
Fragments arranged by size:
3063 2262 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2169 2445 2472 3761 5145 5413 1632
Size: 537 276 27 1289 1384 268 1917
Fragments arranged by size:
1917 1384 1289 537 276 268 27
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5501
AflIII A'CryG_T
Cuts at: 1203 2934 4597 5574 1203
Size: 1731 1663 977 1327
Fragments arranged by size:
1731 1663 1327 977
AgeI A'CCGG_T
Cuts at: 4529 4529
Size: 5698
AhdI GACnn_n'nnGTC
Cuts at: 4427 4427
Size: 5698
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5698
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5698
AlwNI CAG_nnn'CTG
Cuts at: 3441 5013 3441
Size: 1572 4126
ApaI G_GGCC'C
Cuts at: 2537 2759 2537
Size: 222 5476
ApaLI G'TGCA_C
Cuts at: 3755 4911 3755
Size: 1156 4542
ApoI r'AATT_y
Cuts at: 1493 3007 3233 3385 5494 1493
Size: 1514 226 152 2109 1697
Fragments arranged by size:
2109 1697 1514 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4252 160
Size: 872 3220 1606
Fragments arranged by size:
3220 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5698
AvaI C'yCGr_G
Cuts at: 1870 1943 3023 1870
Size: 73 1080 4545
Fragments arranged by size:
4545 1080 73
BamHI G'GATC_C
Cuts at: 3028 3395 3028
Size: 367 5331
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2906 3019 4474 618
Size: 325 217 627 1119 113 1455 1842
Fragments arranged by size:
1842 1455 1119 627 325 217 113
BanII G_rGCy'C
Cuts at: 1391 2084 2266 2490 2537 2759 3017 1391
Size: 693 182 224 47 222 258 4072
Fragments arranged by size:
4072 693 258 224 222 182 47
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2980 928
Size: 971 1081 3646
Fragments arranged by size:
3646 1081 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3889 3923 1903
Size: 34 1952 34 3678
Fragments arranged by size:
3678 1952 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3590 4800 3590
Size: 1210 4488
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5698
BfrBI ATG'CAT
Cuts at: 2305 2305
Size: 5698
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4309 136
Size: 107 122 71 631 605 2637 1525
Fragments arranged by size:
2637 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5693 5693
Size: 5698
BlpI GC'TnA_GC
Cuts at: 2135 3115 2135
Size: 980 4718
Bme1580I G_kGCm'C
Cuts at: 1790 2537 2759 3759 4915 1790
Size: 747 222 1000 1156 2573
Fragments arranged by size:
2573 1156 1000 747 222
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5698
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2540 3429 4387
Size: 416 53 83 399 24 1123 889 958
Cuts at: 4387 442
Size: 1753
Fragments arranged by size:
1753 1123 958 889 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5698
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2453 2573 4358 1239
Size: 1214 120 1785 2579
Fragments arranged by size:
2579 1785 1214 120
Bpu10I CC'TnA_GC
Cuts at: 2774 2872 2774
Size: 98 5600
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2270 2660 3823 4688 4986 2270
Size: 390 1163 865 298 2982
Fragments arranged by size:
2982 1163 865 390 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2817 4361 882
Size: 617 1318 1544 2219
Fragments arranged by size:
2219 1544 1318 617
BsaAI yAC'GTr
Cuts at: 493 1204 3489 493
Size: 711 2285 2702
Fragments arranged by size:
2702 2285 711
BsaBI GATnn'nnATC
Cuts at: 5692 5692
Size: 5698
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2319 3505 3887 4522
Size: 53 83 186 936 786 1186 382 635
Cuts at: 4522 275
Size: 1451
Fragments arranged by size:
1451 1186 936 786 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4131 4529 4803 4950 5361 4131
Size: 398 274 147 411 4468
Fragments arranged by size:
4468 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2031 2061 2462 2492 1178
Size: 30 165 30 628 30 401 30 4384
Fragments arranged by size:
4384 628 401 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5698
BseYI C'CCAG_C
Cuts at: 4901 5397 4901
Size: 496 5202
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5125
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3148 3909 4937 1540
Size: 275 186 1147 761 1028 2301
Fragments arranged by size:
2301 1147 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3759 3844 4915 3759
Size: 85 1071 4542
Fragments arranged by size:
4542 1071 85
BsmI GAATG_Cn'
Cuts at: 2884 3209 3302 2884
Size: 325 93 5280
Fragments arranged by size:
5280 325 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2084 2266 2490 2537 2759 3017 3759
Size: 399 294 182 224 47 222 258 742
Cuts at: 3759 3844 4915 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 294 258 224 222
182 85 47
BspEI T'CCGG_A
Cuts at: 5361 5361
Size: 5698
BspHI T'CATG_A
Cuts at: 3587 5317 3587
Size: 1730 3968
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3053 844
Size: 2209 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3585 1394
Size: 429 1762 3507
Fragments arranged by size:
3507 1762 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4193 4367 65
Size: 1183 2945 174 1396
Fragments arranged by size:
2945 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4342 4529 1700
Size: 110 2532 187 2869
Fragments arranged by size:
2869 2532 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5698
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5698
BssSI C'ACGA_G
Cuts at: 2561 3758 4770 2561
Size: 1197 1012 3489
Fragments arranged by size:
3489 1197 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5698
BstBI TT'CG_AA
Cuts at: 4502 4502
Size: 5698
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5698
BstXI CCAn_nnnn'nTGG
Cuts at: 2035 2035
Size: 5698
BstYI r'GATC_y
Cuts at: 1972 3028 3395 3780 3797 5249 5335 5347 5693
Size: 1056 367 385 17 1452 86 12 346
Cuts at: 5693 1972
Size: 1977
Fragments arranged by size:
1977 1452 1056 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2333 2333
Size: 5698
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5146
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4959
Fragments arranged by size:
4959 683 56
BtsI GCAGTG_nn'
Cuts at: 3214 4008 4028 3214
Size: 794 20 4884
Fragments arranged by size:
4884 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5698
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5663
DraI TTT'AAA
Cuts at: 3002 3002
Size: 5698
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4705 809
Size: 507 3389 1802
Fragments arranged by size:
3389 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2397 2440 3145
Size: 54 1425 50 275 18 567 43 705
Cuts at: 3145 4034 5663 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 705 567 275 54 50
43 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3145 1537
Size: 275 1333 4090
Fragments arranged by size:
4090 1333 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2100 2145 2175 2263 3628 1353
Size: 747 45 30 88 1365 3423
Fragments arranged by size:
3423 1365 747 88 45 30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4285 4659 4805 387
Size: 1064 138 2696 374 146 1280
Fragments arranged by size:
2696 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2169 2445 2453 2472 2573 3761 4358
Size: 393 537 276 8 19 101 1188 597
Cuts at: 4358 5145 5413 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1188 787 597 537 393 276 268
101 19 8
EcoO109I rG'GnC_Cy
Cuts at: 2216 2534 2755 2970 2216
Size: 318 221 215 4944
Fragments arranged by size:
4944 318 221 215
EcoRI G'AATT_C
Cuts at: 3007 3385 3007
Size: 378 5320
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2264 2264
Size: 5698
FspI TGC'GCA
Cuts at: 4204 4204
Size: 5698
HaeII r_GCGC'y
Cuts at: 1678 4845 1678
Size: 3167 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2031 2063 2200 2227 2232
Size: 32 135 32 459 32 137 27 5
Cuts at: 2232 2259 2371 2403 2555 2587 4339 4371 4413
Size: 27 112 32 152 32 1752 32 42
Cuts at: 4413 4445 1373
Size: 32 2626
Fragments arranged by size:
2626 1752 459 152 137 135 112 42
32 32 32 32 32 32 32 27
27 5
HincII GTy'rAC
Cuts at: 669 1657 1938 2050 3042 3288 669
Size: 988 281 112 992 246 3079
Fragments arranged by size:
3079 992 988 281 246 112
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5698
HpaI GTT'AAC
Cuts at: 3288 3288
Size: 5698
KpnI G_GTAC'C
Cuts at: 2910 3023 2910
Size: 113 5585
MfeI C'AATT_G
Cuts at: 3297 3297
Size: 5698
MluI A'CGCG_T
Cuts at: 5574 5574
Size: 5698
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2551 4812 4996 1305
Size: 1246 2261 184 2007
Fragments arranged by size:
2261 2007 1246 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2399 2442 10
Size: 54 1425 343 567 43 3266
Fragments arranged by size:
3266 1425 567 343 54 43
MslI CAynn'nnrTG
Cuts at: 518 1166 3656 4015 4174 518
Size: 648 2490 359 159 2042
Fragments arranged by size:
2490 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3114 3791 4939 5184 1606
Size: 1508 677 1148 245 2120
Fragments arranged by size:
2120 1508 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5146
NdeI CA'TA_TG
Cuts at: 387 2652 387
Size: 2265 3433
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5698
NotI GC'GGCC_GC
Cuts at: 3145 3145
Size: 5698
NsiI A_TGCA'T
Cuts at: 2307 2307
Size: 5698
NspI r_CATG'y
Cuts at: 1132 1229 2882 2938 3056 4601 1132
Size: 97 1653 56 118 1545 2229
Fragments arranged by size:
2229 1653 1545 118 97 56
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5698
PciI A'CATG_T
Cuts at: 2934 4597 2934
Size: 1663 4035
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5698
PfoI T'CCnGG_A
Cuts at: 2060 2430 2550 2060
Size: 370 120 5208
Fragments arranged by size:
5208 370 120
PmeI GTTT'AAAC
Cuts at: 3002 3002
Size: 5698
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3767 3799 5280 5312 3767
Size: 32 1481 32 4153
Fragments arranged by size:
4153 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2216 2970 2216
Size: 754 4944
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5698
PsiI TTA'TAA
Cuts at: 3268 3268
Size: 5698
PspOMI G'GGCC_C
Cuts at: 2533 2755 2533
Size: 222 5476
PstI C_TGCA'G
Cuts at: 830 2738 3050 830
Size: 1908 312 3478
Fragments arranged by size:
3478 1908 312
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5698
SalI G'TCGA_C
Cuts at: 1936 3040 1936
Size: 1104 4594
SanDI GG'GwC_CC
Cuts at: 2216 2970 2216
Size: 754 4944
SapI GCTCTTCn'nnn_
Cuts at: 2145 2145
Size: 5698
SbfI CC_TGCA'GG
Cuts at: 3050 3050
Size: 5698
ScaI AGT'ACT
Cuts at: 3946 5658 3946
Size: 1712 3986
SexAI A'CCwGG_T
Cuts at: 2116 2508 2116
Size: 392 5306
SfcI C'TryA_G
Cuts at: 826 1045 2734 2833 2928 3046 4181 4862 5053
Size: 219 1689 99 95 118 1135 681 191
Cuts at: 5053 826
Size: 1471
Fragments arranged by size:
1689 1471 1135 681 219 191 118 99
95
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5698
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5698
SmaI CCC'GGG
Cuts at: 1872 3025 1872
Size: 1153 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2249 2675 3802 4703 4965 820
Size: 197 926 306 426 1127 901 262 1553
Fragments arranged by size:
1553 1127 926 901 426 306 262 197
SnaBI TAC'GTA
Cuts at: 493 3489 493
Size: 2996 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5698
SphI G_CATG'C
Cuts at: 1132 3056 1132
Size: 1924 3774
SspI AAT'ATT
Cuts at: 5 52 3622 5
Size: 47 3570 2081
Fragments arranged by size:
3570 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5698
StyI C'CwwG_G
Cuts at: 513 1065 2028 3137 513
Size: 552 963 1109 3074
Fragments arranged by size:
3074 1109 963 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3725 3742 3895 4080 4525 325
Size: 1255 2145 17 153 185 445 1498
Fragments arranged by size:
2145 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2738 3944 5656
Size: 274 80 33 51 1443 761 1206 1712
Cuts at: 5656 96
Size: 138
Fragments arranged by size:
1712 1443 1206 761 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2462 2494 5658 5690 2462
Size: 32 3164 32 2470
Fragments arranged by size:
3164 2470 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5698
XbaI T'CTAG_A
Cuts at: 3034 3034
Size: 5698
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2506 2963 2506
Size: 457 5241
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5698
XmaI C'CCGG_G
Cuts at: 1870 3023 1870
Size: 1153 4545
XmnI GAAnn'nnTTC
Cuts at: 3827 3827
Size: 5698
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3506 276
Size: 53 83 186 936 1972 2468
Fragments arranged by size:
2468 1972 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI SalI SanDI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PmlI PspXI PsrI PvuII RsrII SacI SacII SfiI
SfoI SrfI SwaI