Restriction map for
pFN21AB9627
(Circular) (Six-base) MAPSORT of: pFN21AB9627.seq Check: 1107 from: 1 to: 5851
pFN21AB9627 5851 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3661 278
Size: 53 83 186 936 2125 2468
Fragments arranged by size:
2468 2125 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3194 1937
Size: 1257 4594
Acc65I G'GTAC_C
Cuts at: 3172 3172
Size: 5851
AclI AA'CG_TT
Cuts at: 1563 3150 3978 4351 1563
Size: 1587 828 373 3063
Fragments arranged by size:
3063 1587 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3914 5298 5566 1632
Size: 2282 1384 268 1917
Fragments arranged by size:
2282 1917 1384 268
AfeI AGC'GCT
Cuts at: 2357 2357
Size: 5851
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5654
AflIII A'CryG_T
Cuts at: 1203 4750 5727 1203
Size: 3547 977 1327
Fragments arranged by size:
3547 1327 977
AgeI A'CCGG_T
Cuts at: 4682 4682
Size: 5851
AhdI GACnn_n'nnGTC
Cuts at: 2560 4580 2560
Size: 2020 3831
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5851
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2420 2462 1184
Size: 1236 42 4573
Fragments arranged by size:
4573 1236 42
AlwNI CAG_nnn'CTG
Cuts at: 2473 2578 3594 5166 2473
Size: 105 1016 1572 3158
Fragments arranged by size:
3158 1572 1016 105
ApaI G_GGCC'C
Cuts at: 2322 2897 3116 2322
Size: 575 219 5057
Fragments arranged by size:
5057 575 219
ApaLI G'TGCA_C
Cuts at: 3908 5064 3908
Size: 1156 4695
ApoI r'AATT_y
Cuts at: 1493 3160 3386 3538 5647 1493
Size: 1667 226 152 2109 1697
Fragments arranged by size:
2109 1697 1667 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4405 160
Size: 872 3373 1606
Fragments arranged by size:
3373 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5851
AvaI C'yCGr_G
Cuts at: 1870 1943 2135 2160 2692 3176 1870
Size: 73 192 25 532 484 4545
Fragments arranged by size:
4545 532 484 192 73 25
BamHI G'GATC_C
Cuts at: 3181 3548 3181
Size: 367 5484
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2446 2663 2672 3172 4627
Size: 325 217 627 659 217 9 500 1455
Cuts at: 4627 618
Size: 1842
Fragments arranged by size:
1842 1455 659 627 500 325 217 217
9
BanII G_rGCy'C
Cuts at: 1391 2031 2149 2322 2867 2897 3116 3132 3170
Size: 640 118 173 545 30 219 16 38
Cuts at: 3170 1391
Size: 4072
Fragments arranged by size:
4072 640 545 219 173 118 38 30
16
BbeI G_GCGC'C
Cuts at: 2667 2667
Size: 5851
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2085 3025 928
Size: 971 186 940 3754
Fragments arranged by size:
3754 971 940 186
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4042 4076 1903
Size: 34 50 34 2021 34 3678
Fragments arranged by size:
3678 2021 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3743 4953 3743
Size: 1210 4641
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5851
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2165 2213 2745
Size: 107 122 71 631 605 493 48 532
Cuts at: 2745 2778 4462 136
Size: 33 1684 1525
Fragments arranged by size:
1684 1525 631 605 532 493 122 107
71 48 33
BglII A'GATC_T
Cuts at: 5846 5846
Size: 5851
BlpI GC'TnA_GC
Cuts at: 2900 3268 2900
Size: 368 5483
Bme1580I G_kGCm'C
Cuts at: 1790 2052 2322 2451 2897 3116 3912 5068 1790
Size: 262 270 129 446 219 796 1156 2573
Fragments arranged by size:
2573 1156 796 446 270 262 219 129
BmgBI CAC'GTC
Cuts at: 1318 2047 1318
Size: 729 5122
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3582 4540 442
Size: 416 53 83 399 24 2165 958 1753
Fragments arranged by size:
2165 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5851
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4511 1239
Size: 3272 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2921 3976 4841 5139 2921
Size: 1055 865 298 3633
Fragments arranged by size:
3633 1055 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4514 882
Size: 617 3015 2219
Fragments arranged by size:
3015 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2569 3642 493
Size: 711 1365 1073 2702
Fragments arranged by size:
2702 1365 1073 711
BsaBI GATnn'nnATC
Cuts at: 5845 5845
Size: 5851
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2664 3060 3658 4040
Size: 53 83 186 936 1131 396 598 382
Cuts at: 4040 4675 275
Size: 635 1451
Fragments arranged by size:
1451 1131 936 635 598 396 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4284 4682 4956 5103 5514 4284
Size: 398 274 147 411 4621
Fragments arranged by size:
4621 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2195 2225 2864 2894 1178
Size: 30 165 30 792 30 639 30 4135
Fragments arranged by size:
4135 792 639 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2107 2264 2267 2270 2279 2282 2938 3140
Size: 921 157 3 3 9 3 656 202
Cuts at: 3140 1186
Size: 3897
Fragments arranged by size:
3897 921 656 202 157 9 3 3
3
BseYI C'CCAG_C
Cuts at: 2064 2239 5054 5550 2064
Size: 175 2815 496 2365
Fragments arranged by size:
2815 2365 496 175
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2591 1163
Size: 573 855 4423
Fragments arranged by size:
4423 855 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2680 2766 3109 3301 4062 5090
Size: 275 186 679 86 343 192 761 1028
Cuts at: 5090 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 679 343 275 192 186
86
BsiHKAI G_wGCw'C
Cuts at: 2735 3132 3912 3997 5068 2735
Size: 397 780 85 1071 3518
Fragments arranged by size:
3518 1071 780 397 85
BsmI GAATG_Cn'
Cuts at: 3362 3455 3362
Size: 93 5758
BsmBI CGTCTCn'nnnn_
Cuts at: 2480 2822 2480
Size: 342 5509
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2031 2052 2149 2322 2451 2735 2867
Size: 399 241 21 97 173 129 284 132
Cuts at: 2867 2897 3116 3132 3170 3912 3997 5068 1391
Size: 30 219 16 38 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 284 241 219 173
132 129 97 85 38 30 21 16
BspEI T'CCGG_A
Cuts at: 5514 5514
Size: 5851
BspHI T'CATG_A
Cuts at: 3740 5470 3740
Size: 1730 4121
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2442 3206 844
Size: 1598 764 3489
Fragments arranged by size:
3489 1598 764
BsrBI CCG'CTC
Cuts at: 1394 1823 2302 2899 2911 3738 1394
Size: 429 479 597 12 827 3507
Fragments arranged by size:
3507 827 597 479 429 12
BsrDI GCAATG_nn'
Cuts at: 65 1248 4346 4520 65
Size: 1183 3098 174 1396
Fragments arranged by size:
3098 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2618 2675 2765 4495 4682 1700
Size: 110 808 57 90 1730 187 2869
Fragments arranged by size:
2869 1730 808 187 110 90 57
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5851
BssHII G'CGCG_C
Cuts at: 1925 2345 1925
Size: 420 5431
BssSI C'ACGA_G
Cuts at: 2960 3911 4923 2960
Size: 951 1012 3888
Fragments arranged by size:
3888 1012 951
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2797 794
Size: 2003 3848
BstBI TT'CG_AA
Cuts at: 4655 4655
Size: 5851
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5851
BstXI CCAn_nnnn'nTGG
Cuts at: 2611 2614 2611
Size: 3 5848
BstYI r'GATC_y
Cuts at: 1972 2529 3181 3548 3933 3950 5402 5488 5500
Size: 557 652 367 385 17 1452 86 12
Cuts at: 5500 5846 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 652 557 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2185 2686 2806 3015 3022 513
Size: 552 1120 501 120 209 7 3342
Fragments arranged by size:
3342 1120 552 501 209 120 7
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5112
Fragments arranged by size:
5112 683 56
BtsI GCAGTG_nn'
Cuts at: 3367 4161 4181 3367
Size: 794 20 5037
Fragments arranged by size:
5037 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5851
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5816
DraI TTT'AAA
Cuts at: 3155 3155
Size: 5851
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4858 809
Size: 507 3542 1802
Fragments arranged by size:
3542 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2166 2214 2549
Size: 54 1425 50 275 18 336 48 335
Cuts at: 2549 2616 2677 2763 3298 4187 5816 8
Size: 67 61 86 535 889 1629 43
Fragments arranged by size:
1629 1425 889 535 336 335 275 86
67 61 54 50 48 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2677 2763 3298 1537
Size: 275 865 86 535 4090
Fragments arranged by size:
4090 865 535 275 86
EarI CTCTTCn'nnn_
Cuts at: 1353 2187 2256 3781 1353
Size: 834 69 1525 3423
Fragments arranged by size:
3423 1525 834 69
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2306 4438 4812 4958 387
Size: 1064 138 717 2132 374 146 1280
Fragments arranged by size:
2132 1280 1064 717 374 146 138
EcoICRI GAG'CTC
Cuts at: 3130 3130
Size: 5851
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3914 4511 5298 5566 1239
Size: 393 2282 597 787 268 1524
Fragments arranged by size:
2282 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2438 2438
Size: 5851
EcoO109I rG'GnC_Cy
Cuts at: 2893 3112 3113 2893
Size: 219 1 5631
Fragments arranged by size:
5631 219 1
EcoRI G'AATT_C
Cuts at: 3160 3538 3160
Size: 378 5473
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2365 2365
Size: 5851
FspI TGC'GCA
Cuts at: 2438 4357 2438
Size: 1919 3932
HaeII r_GCGC'y
Cuts at: 1678 2359 2522 2667 2999 4998 1678
Size: 681 163 145 332 1999 2531
Fragments arranged by size:
2531 1999 681 332 163 145
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2546 2578 4492 4524 4566
Size: 32 135 32 974 32 1914 32 42
Cuts at: 4566 4598 1373
Size: 32 2626
Fragments arranged by size:
2626 1914 974 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3195 3441 669
Size: 988 281 1257 246 3079
Fragments arranged by size:
3079 1257 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2363 748
Size: 1615 4236
HpaI GTT'AAC
Cuts at: 3441 3441
Size: 5851
KasI G'GCGC_C
Cuts at: 2663 2663
Size: 5851
KpnI G_GTAC'C
Cuts at: 3176 3176
Size: 5851
MfeI C'AATT_G
Cuts at: 3450 3450
Size: 5851
MluI A'CGCG_T
Cuts at: 5727 5727
Size: 5851
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4965 5149 1305
Size: 3660 184 2007
Fragments arranged by size:
3660 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4029
Fragments arranged by size:
4029 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3809 4168 4327 518
Size: 648 2643 359 159 2042
Fragments arranged by size:
2643 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2064 3267 3944 5092 5337 1606
Size: 458 1203 677 1148 245 2120
Fragments arranged by size:
2120 1203 1148 677 458 245
NaeI GCC'GGC
Cuts at: 2620 2677 2767 2620
Size: 57 90 5704
Fragments arranged by size:
5704 90 57
NarI GG'CG_CC
Cuts at: 2664 2664
Size: 5851
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5299
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5851
NgoMIV G'CCGG_C
Cuts at: 2618 2675 2765 2618
Size: 57 90 5704
Fragments arranged by size:
5704 90 57
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5851
NotI GC'GGCC_GC
Cuts at: 3298 3298
Size: 5851
NspI r_CATG'y
Cuts at: 1132 1229 2407 3209 4754 1132
Size: 97 1178 802 1545 2229
Fragments arranged by size:
2229 1545 1178 802 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5851
PciI A'CATG_T
Cuts at: 4750 4750
Size: 5851
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5851
PmeI GTTT'AAAC
Cuts at: 3155 3155
Size: 5851
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3920 3952 5433 5465 3920
Size: 32 1481 32 4306
Fragments arranged by size:
4306 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5851
PsiI TTA'TAA
Cuts at: 3421 3421
Size: 5851
PspOMI G'GGCC_C
Cuts at: 2318 2893 3112 2318
Size: 575 219 5057
Fragments arranged by size:
5057 575 219
PstI C_TGCA'G
Cuts at: 830 2631 3203 830
Size: 1801 572 3478
Fragments arranged by size:
3478 1801 572
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5851
PvuII CAG'CTG
Cuts at: 2064 2064
Size: 5851
SacI G_AGCT'C
Cuts at: 3132 3132
Size: 5851
SalI G'TCGA_C
Cuts at: 1936 3193 1936
Size: 1257 4594
SbfI CC_TGCA'GG
Cuts at: 3203 3203
Size: 5851
ScaI AGT'ACT
Cuts at: 4099 5811 4099
Size: 1712 4139
SfcI C'TryA_G
Cuts at: 826 1045 2627 3199 4334 5015 5206 826
Size: 219 1582 572 1135 681 191 1471
Fragments arranged by size:
1582 1471 1135 681 572 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2213 2213
Size: 5851
SfoI GGC'GCC
Cuts at: 2665 2665
Size: 5851
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5851
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5851
SmaI CCC'GGG
Cuts at: 1872 2137 2162 3178 1872
Size: 265 25 1016 4545
Fragments arranged by size:
4545 1016 265 25
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2936 3955 4856 5118 820
Size: 197 926 993 1019 901 262 1553
Fragments arranged by size:
1553 1019 993 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3642 493
Size: 3149 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5851
SphI G_CATG'C
Cuts at: 1132 2407 3209 1132
Size: 1275 802 3774
Fragments arranged by size:
3774 1275 802
SrfI GCCC'GGGC
Cuts at: 2137 2162 2137
Size: 25 5826
SspI AAT'ATT
Cuts at: 5 52 3775 5
Size: 47 3723 2081
Fragments arranged by size:
3723 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5851
StyI C'CwwG_G
Cuts at: 513 1065 2340 3290 513
Size: 552 1275 950 3074
Fragments arranged by size:
3074 1275 950 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3878 3895 4048 4233 4678 325
Size: 1255 2298 17 153 185 445 1498
Fragments arranged by size:
2298 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4097 5809 96
Size: 274 80 33 51 1443 2120 1712 138
Fragments arranged by size:
2120 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5811 5843 5811
Size: 32 5819
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5851
XbaI T'CTAG_A
Cuts at: 3187 3187
Size: 5851
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2347 2608 2611 2347
Size: 261 3 5587
Fragments arranged by size:
5587 261 3
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5851
XmaI C'CCGG_G
Cuts at: 1870 2135 2160 3176 1870
Size: 265 25 1016 4545
Fragments arranged by size:
4545 1016 265 25
XmnI GAAnn'nnTTC
Cuts at: 3980 3980
Size: 5851
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3659 276
Size: 53 83 186 936 2125 2468
Fragments arranged by size:
2468 2125 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SalI SbfI ScaI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoRV FseI FspAI
NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI
RsrII SacII SanDI SapI SexAI SwaI