Restriction map for
pFN21AB9625
(Circular) (Six-base) MAPSORT of: pFN21AB9625.seq Check: 6942 from: 1 to: 5104
pFN21AB9625 5104 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2403 2914 278
Size: 53 83 186 936 867 511 2468
Fragments arranged by size:
2468 936 867 511 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2447 1937
Size: 510 4594
Acc65I G'GTAC_C
Cuts at: 2425 2425
Size: 5104
AclI AA'CG_TT
Cuts at: 1563 3231 3604 1563
Size: 1668 373 3063
Fragments arranged by size:
3063 1668 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2056 3167 4551 4819 1632
Size: 424 1111 1384 268 1917
Fragments arranged by size:
1917 1384 1111 424 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4907
AflIII A'CryG_T
Cuts at: 1203 4003 4980 1203
Size: 2800 977 1327
Fragments arranged by size:
2800 1327 977
AgeI A'CCGG_T
Cuts at: 3935 3935
Size: 5104
AhdI GACnn_n'nnGTC
Cuts at: 3833 3833
Size: 5104
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5104
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2324 1184
Size: 1140 3964
AlwNI CAG_nnn'CTG
Cuts at: 2060 2079 2185 2847 4419 2060
Size: 19 106 662 1572 2745
Fragments arranged by size:
2745 1572 662 106 19
ApaI G_GGCC'C
Cuts at: 2099 2099
Size: 5104
ApaLI G'TGCA_C
Cuts at: 3161 4317 3161
Size: 1156 3948
ApoI r'AATT_y
Cuts at: 1493 2413 2639 2791 4900 1493
Size: 920 226 152 2109 1697
Fragments arranged by size:
2109 1697 920 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3658 160
Size: 872 2626 1606
Fragments arranged by size:
2626 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5104
AvaI C'yCGr_G
Cuts at: 1870 1943 2139 2429 1870
Size: 73 196 290 4545
Fragments arranged by size:
4545 290 196 73
BamHI G'GATC_C
Cuts at: 2434 2801 2434
Size: 367 4737
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2105 2205 2211 2425 3880
Size: 325 217 627 318 100 6 214 1455
Cuts at: 3880 618
Size: 1842
Fragments arranged by size:
1842 1455 627 325 318 217 214 100
6
BanII G_rGCy'C
Cuts at: 1391 2099 2423 1391
Size: 708 324 4072
Fragments arranged by size:
4072 708 324
BbeI G_GCGC'C
Cuts at: 2209 2215 2209
Size: 6 5098
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4133
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3295 3329 1903
Size: 34 1358 34 3678
Fragments arranged by size:
3678 1358 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2996 4206 2996
Size: 1210 3894
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5104
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3715 136
Size: 107 122 71 631 605 2043 1525
Fragments arranged by size:
2043 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5099 5099
Size: 5104
BlpI GC'TnA_GC
Cuts at: 2521 2521
Size: 5104
Bme1580I G_kGCm'C
Cuts at: 1790 2099 3165 4321 1790
Size: 309 1066 1156 2573
Fragments arranged by size:
2573 1156 1066 309
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5104
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2835 3793 442
Size: 416 53 83 399 24 1418 958 1753
Fragments arranged by size:
1753 1418 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5104
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3764 1239
Size: 2525 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3229 4094 4392 3229
Size: 865 298 3941
Fragments arranged by size:
3941 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3767 882
Size: 617 2268 2219
Fragments arranged by size:
2268 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2392 2895 493
Size: 711 1188 503 2702
Fragments arranged by size:
2702 1188 711 503
BsaBI GATnn'nnATC
Cuts at: 5098 5098
Size: 5104
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2206 2212 2400 2911
Size: 53 83 186 936 673 6 188 511
Cuts at: 2911 3293 3928 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 673 635 511 382 188 186
83 53 6
BsaWI w'CCGG_w
Cuts at: 3537 3935 4209 4356 4767 3537
Size: 398 274 147 411 3874
Fragments arranged by size:
3874 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4879
Fragments arranged by size:
4879 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2210 1186
Size: 1024 4080
BseYI C'CCAG_C
Cuts at: 4307 4803 4307
Size: 496 4608
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4531
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2554 3315 4343 1540
Size: 275 186 553 761 1028 2301
Fragments arranged by size:
2301 1028 761 553 275 186
BsiHKAI G_wGCw'C
Cuts at: 3165 3250 4321 3165
Size: 85 1071 3948
Fragments arranged by size:
3948 1071 85
BsmI GAATG_Cn'
Cuts at: 2615 2708 2615
Size: 93 5011
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2099 2423 3165 3250 4321 1391
Size: 399 309 324 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 324 309 85
BspEI T'CCGG_A
Cuts at: 4767 4767
Size: 5104
BspHI T'CATG_A
Cuts at: 2993 4723 2993
Size: 1730 3374
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2046 2184 2459 844
Size: 1202 138 275 3489
Fragments arranged by size:
3489 1202 275 138
BsrBI CCG'CTC
Cuts at: 1394 1823 2991 1394
Size: 429 1168 3507
Fragments arranged by size:
3507 1168 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3599 3773 65
Size: 1183 2351 174 1396
Fragments arranged by size:
2351 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2202 2208 3748 3935 1700
Size: 110 392 6 1540 187 2869
Fragments arranged by size:
2869 1540 392 187 110 6
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5104
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5104
BssSI C'ACGA_G
Cuts at: 3164 4176 3164
Size: 1012 4092
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5104
BstBI TT'CG_AA
Cuts at: 3908 3908
Size: 5104
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5104
BstXI CCAn_nnnn'nTGG
Cuts at: 2032 2032
Size: 5104
BstYI r'GATC_y
Cuts at: 1972 2434 2801 3186 3203 4655 4741 4753 5099
Size: 462 367 385 17 1452 86 12 346
Cuts at: 5099 1972
Size: 1977
Fragments arranged by size:
1977 1452 462 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2025 513
Size: 552 960 3592
Fragments arranged by size:
3592 960 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4365
Fragments arranged by size:
4365 683 56
BtsI GCAGTG_nn'
Cuts at: 2620 3414 3434 2620
Size: 794 20 4290
Fragments arranged by size:
4290 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5104
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5069
DraI TTT'AAA
Cuts at: 2408 2408
Size: 5104
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4111 809
Size: 507 2795 1802
Fragments arranged by size:
2795 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2164 2361 2551
Size: 54 1425 50 275 18 334 197 190
Cuts at: 2551 3440 5069 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 334 275 197 190 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2551 1537
Size: 275 739 4090
Fragments arranged by size:
4090 739 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3034 1353
Size: 1681 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3691 4065 4211 387
Size: 1064 138 2102 374 146 1280
Fragments arranged by size:
2102 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2056 3167 3764 4551 4819 1239
Size: 393 424 1111 597 787 268 1524
Fragments arranged by size:
1524 1111 787 597 424 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2096 2096
Size: 5104
EcoRI G'AATT_C
Cuts at: 2413 2791 2413
Size: 378 4726
FspI TGC'GCA
Cuts at: 2300 3610 2300
Size: 1310 3794
HaeII r_GCGC'y
Cuts at: 1678 2203 2209 2215 4251 1678
Size: 525 6 6 2036 2531
Fragments arranged by size:
2531 2036 525 6 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2048 2080 2244 2276 3745
Size: 32 135 32 476 32 164 32 1469
Cuts at: 3745 3777 3819 3851 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1469 476 164 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2448 2694 669
Size: 988 281 510 246 3079
Fragments arranged by size:
3079 988 510 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5104
HpaI GTT'AAC
Cuts at: 2694 2694
Size: 5104
KasI G'GCGC_C
Cuts at: 2205 2211 2205
Size: 6 5098
KpnI G_GTAC'C
Cuts at: 2429 2429
Size: 5104
MfeI C'AATT_G
Cuts at: 2703 2703
Size: 5104
MluI A'CGCG_T
Cuts at: 4980 4980
Size: 5104
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4218 4402 1305
Size: 2913 184 2007
Fragments arranged by size:
2913 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2363 10
Size: 54 1425 343 531 2751
Fragments arranged by size:
2751 1425 531 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2264 3062 3421 3580 518
Size: 648 1098 798 359 159 2042
Fragments arranged by size:
2042 1098 798 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2520 3197 4345 4590 1606
Size: 914 677 1148 245 2120
Fragments arranged by size:
2120 1148 914 677 245
NaeI GCC'GGC
Cuts at: 2204 2210 2204
Size: 6 5098
NarI GG'CG_CC
Cuts at: 2206 2212 2206
Size: 6 5098
NcoI C'CATG_G
Cuts at: 513 1065 2025 513
Size: 552 960 3592
Fragments arranged by size:
3592 960 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5104
NgoMIV G'CCGG_C
Cuts at: 2202 2208 2202
Size: 6 5098
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5104
NotI GC'GGCC_GC
Cuts at: 2551 2551
Size: 5104
NspI r_CATG'y
Cuts at: 1132 1229 2462 4007 1132
Size: 97 1233 1545 2229
Fragments arranged by size:
2229 1545 1233 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5104
PciI A'CATG_T
Cuts at: 4003 4003
Size: 5104
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2395 1108
Size: 1287 3817
PfoI T'CCnGG_A
Cuts at: 2122 2122
Size: 5104
PmeI GTTT'AAAC
Cuts at: 2408 2408
Size: 5104
PmlI CAC'GTG
Cuts at: 2392 2392
Size: 5104
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3173 3205 4686 4718 3173
Size: 32 1481 32 3559
Fragments arranged by size:
3559 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5104
PsiI TTA'TAA
Cuts at: 2674 2674
Size: 5104
PspOMI G'GGCC_C
Cuts at: 2095 2095
Size: 5104
PstI C_TGCA'G
Cuts at: 830 2181 2456 830
Size: 1351 275 3478
Fragments arranged by size:
3478 1351 275
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5104
SalI G'TCGA_C
Cuts at: 1936 2446 1936
Size: 510 4594
SbfI CC_TGCA'GG
Cuts at: 2456 2456
Size: 5104
ScaI AGT'ACT
Cuts at: 3352 5064 3352
Size: 1712 3392
SfcI C'TryA_G
Cuts at: 826 1045 2177 2452 3587 4268 4459 826
Size: 219 1132 275 1135 681 191 1471
Fragments arranged by size:
1471 1135 1132 681 275 219 191
SfoI GGC'GCC
Cuts at: 2207 2213 2207
Size: 6 5098
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5104
SgrAI Cr'CCGG_yG
Cuts at: 1700 2202 2208 1700
Size: 502 6 4596
Fragments arranged by size:
4596 502 6
SmaI CCC'GGG
Cuts at: 1872 2431 1872
Size: 559 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3208 4109 4371 820
Size: 197 926 1265 901 262 1553
Fragments arranged by size:
1553 1265 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2895 493
Size: 2402 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5104
SphI G_CATG'C
Cuts at: 1132 2462 1132
Size: 1330 3774
SspI AAT'ATT
Cuts at: 5 52 3028 5
Size: 47 2976 2081
Fragments arranged by size:
2976 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5104
StyI C'CwwG_G
Cuts at: 513 1065 2025 2543 513
Size: 552 960 518 3074
Fragments arranged by size:
3074 960 552 518
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2063 3131 3148 3301 3486 3931 325
Size: 1255 483 1068 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1068 483 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3350 5062 96
Size: 274 80 33 51 1443 1373 1712 138
Fragments arranged by size:
1712 1443 1373 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5064 5096 5064
Size: 32 5072
Tth111I GACn'n_nGTC
Cuts at: 1316 2062 1316
Size: 746 4358
XbaI T'CTAG_A
Cuts at: 2440 2440
Size: 5104
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5104
XmaI C'CCGG_G
Cuts at: 1870 2429 1870
Size: 559 4545
XmnI GAAnn'nnTTC
Cuts at: 3233 3233
Size: 5104
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2401 2912 276
Size: 53 83 186 936 867 511 2468
Fragments arranged by size:
2468 936 867 511 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI
PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SrfI SwaI XcmI