Restriction map for
pFN21AB9621
(Circular) (Six-base) MAPSORT of: pFN21AB9621.seq Check: 6787 from: 1 to: 6757
pFN21AB9621 6757 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2025 2506 4567 278
Size: 53 83 186 936 489 481 2061 2468
Fragments arranged by size:
2468 2061 936 489 481 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2382 3612 4100 1937
Size: 445 1230 488 4594
Fragments arranged by size:
4594 1230 488 445
Acc65I G'GTAC_C
Cuts at: 3800 4078 3800
Size: 278 6479
AclI AA'CG_TT
Cuts at: 1563 4884 5257 1563
Size: 3321 373 3063
Fragments arranged by size:
3321 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2838 4820 6204 6472 1632
Size: 1206 1982 1384 268 1917
Fragments arranged by size:
1982 1917 1384 1206 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6560
AflIII A'CryG_T
Cuts at: 1203 5656 6633 1203
Size: 4453 977 1327
Fragments arranged by size:
4453 1327 977
AgeI A'CCGG_T
Cuts at: 5588 5588
Size: 6757
AhdI GACnn_n'nnGTC
Cuts at: 2362 3967 5486 2362
Size: 1605 1519 3633
Fragments arranged by size:
3633 1605 1519
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6757
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3273 1184
Size: 2089 4668
AlwNI CAG_nnn'CTG
Cuts at: 2434 2671 2743 3016 4500 6072 2434
Size: 237 72 273 1484 1572 3119
Fragments arranged by size:
3119 1572 1484 273 237 72
ApaI G_GGCC'C
Cuts at: 2899 2899
Size: 6757
ApaLI G'TGCA_C
Cuts at: 4814 5970 4814
Size: 1156 5601
ApoI r'AATT_y
Cuts at: 1493 4066 4292 4444 6553 1493
Size: 2573 226 152 2109 1697
Fragments arranged by size:
2573 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3792 5311 160
Size: 872 2760 1519 1606
Fragments arranged by size:
2760 1606 1519 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6757
AvaI C'yCGr_G
Cuts at: 1870 1943 2013 3091 4082 1870
Size: 73 70 1078 991 4545
Fragments arranged by size:
4545 1078 991 73 70
BamHI G'GATC_C
Cuts at: 4087 4454 4087
Size: 367 6390
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2938 3800 4078 5533 618
Size: 325 217 627 1151 862 278 1455 1842
Fragments arranged by size:
1842 1455 1151 862 627 325 278 217
BanII G_rGCy'C
Cuts at: 1391 2899 4076 1391
Size: 1508 1177 4072
Fragments arranged by size:
4072 1508 1177
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2025 2779 3556 928
Size: 971 126 754 777 4129
Fragments arranged by size:
4129 971 777 754 126
BbvCI CC'TCA_GC
Cuts at: 3157 4023 3157
Size: 866 5891
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4948 4982 1903
Size: 34 50 34 2927 34 3678
Fragments arranged by size:
3678 2927 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4649 5859 4649
Size: 1210 5547
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6757
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2153 5368 136
Size: 107 122 71 631 605 481 3215 1525
Fragments arranged by size:
3215 1525 631 605 481 122 107 71
BglII A'GATC_T
Cuts at: 2650 6752 2650
Size: 4102 2655
BlpI GC'TnA_GC
Cuts at: 2612 3201 4174 2612
Size: 589 973 5195
Fragments arranged by size:
5195 973 589
Bme1580I G_kGCm'C
Cuts at: 1790 2899 4818 5974 1790
Size: 1109 1919 1156 2573
Fragments arranged by size:
2573 1919 1156 1109
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6757
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2575 2766 3026
Size: 416 53 83 399 24 1158 191 260
Cuts at: 3026 3593 4488 5446 442
Size: 567 895 958 1753
Fragments arranged by size:
1753 1158 958 895 567 416 399 260
191 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2273 1056
Size: 1217 5540
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3979 3979
Size: 6757
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2765 3366 5417 1239
Size: 1526 601 2051 2579
Fragments arranged by size:
2579 2051 1526 601
Bpu10I CC'TnA_GC
Cuts at: 3157 4023 3157
Size: 866 5891
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2147 2519 4882 5747 6045 2147
Size: 372 2363 865 298 2859
Fragments arranged by size:
2859 2363 865 372 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3327 3662 5420 882
Size: 617 1828 335 1758 2219
Fragments arranged by size:
2219 1828 1758 617 335
BsaAI yAC'GTr
Cuts at: 493 1204 4548 493
Size: 711 3344 2702
Fragments arranged by size:
3344 2702 711
BsaBI GATnn'nnATC
Cuts at: 3041 6751 3041
Size: 3710 3047
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2022 2216 2358 2503
Size: 53 83 186 936 489 194 142 145
Cuts at: 2503 2547 4564 4946 5581 275
Size: 44 2017 382 635 1451
Fragments arranged by size:
2017 1451 936 635 489 382 194 186
145 142 83 53 44
BsaWI w'CCGG_w
Cuts at: 5190 5588 5862 6009 6420 5190
Size: 398 274 147 411 5527
Fragments arranged by size:
5527 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2429 2459 1178
Size: 30 165 30 1026 30 5476
Fragments arranged by size:
5476 1026 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2132 2651 3145 1186
Size: 946 519 494 4798
Fragments arranged by size:
4798 946 519 494
BseYI C'CCAG_C
Cuts at: 2664 3619 5960 6456 2664
Size: 955 2341 496 2965
Fragments arranged by size:
2965 2341 955 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2447 2506 2756 1163
Size: 573 711 59 250 5164
Fragments arranged by size:
5164 711 573 250 59
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4207 4968 5996 1540
Size: 275 186 2206 761 1028 2301
Fragments arranged by size:
2301 2206 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3853 4818 4903 5974 3853
Size: 965 85 1071 4636
Fragments arranged by size:
4636 1071 965 85
BsmI GAATG_Cn'
Cuts at: 3374 4268 4361 3374
Size: 894 93 5770
Fragments arranged by size:
5770 894 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2899 3853 4076 4818 4903 5974 1391
Size: 399 1109 954 223 742 85 1071 2174
Fragments arranged by size:
2174 1109 1071 954 742 399 223 85
BspEI T'CCGG_A
Cuts at: 6420 6420
Size: 6757
BspHI T'CATG_A
Cuts at: 3603 3694 4646 6376 3603
Size: 91 952 1730 3984
Fragments arranged by size:
3984 1730 952 91
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4112 844
Size: 3268 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2713 2764 4644 1394
Size: 429 890 51 1880 3507
Fragments arranged by size:
3507 1880 890 429 51
BsrDI GCAATG_nn'
Cuts at: 65 1248 5252 5426 65
Size: 1183 4004 174 1396
Fragments arranged by size:
4004 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5401 5588 1700
Size: 110 3591 187 2869
Fragments arranged by size:
3591 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2925 96
Size: 2829 3928
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6757
BssSI C'ACGA_G
Cuts at: 3852 4817 5829 3852
Size: 965 1012 4780
Fragments arranged by size:
4780 1012 965
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3489 794
Size: 2695 4062
BstBI TT'CG_AA
Cuts at: 5561 5561
Size: 6757
BstEII G'GTnAC_C
Cuts at: 1184 2915 1184
Size: 1731 5026
BstXI CCAn_nnnn'nTGG
Cuts at: 2209 3037 2209
Size: 828 5929
BstYI r'GATC_y
Cuts at: 1972 2650 3064 4087 4454 4839 4856 6308 6394
Size: 678 414 1023 367 385 17 1452 86
Cuts at: 6394 6406 6752 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1023 678 414 385 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2053 513
Size: 552 988 5217
Fragments arranged by size:
5217 988 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6018
Fragments arranged by size:
6018 683 56
BtsI GCAGTG_nn'
Cuts at: 2744 4273 5067 5087 2744
Size: 1529 794 20 4414
Fragments arranged by size:
4414 1529 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6757
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6722
DraI TTT'AAA
Cuts at: 4061 4061
Size: 6757
DraIII CAC_nnn'GTG
Cuts at: 2353 3917 2353
Size: 1564 5193
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3599 5764 809
Size: 507 2283 2165 1802
Fragments arranged by size:
2283 2165 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4204 5093 6722
Size: 54 1425 50 275 18 2374 889 1629
Cuts at: 6722 8
Size: 43
Fragments arranged by size:
2374 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4204 1537
Size: 275 2392 4090
Fragments arranged by size:
4090 2392 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4687 1353
Size: 3334 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3655 5344 5718 5864 387
Size: 1064 138 2066 1689 374 146 1280
Fragments arranged by size:
2066 1689 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2765 2838 3366 4820 5417 6204 6472
Size: 393 1133 73 528 1454 597 787 268
Cuts at: 6472 1239
Size: 1524
Fragments arranged by size:
1524 1454 1133 787 597 528 393 268
73
EcoO109I rG'GnC_Cy
Cuts at: 2768 2895 2896 2768
Size: 127 1 6629
Fragments arranged by size:
6629 127 1
EcoRI G'AATT_C
Cuts at: 4066 4444 4066
Size: 378 6379
FspI TGC'GCA
Cuts at: 5263 5263
Size: 6757
HaeII r_GCGC'y
Cuts at: 1678 2434 5904 1678
Size: 756 3470 2531
Fragments arranged by size:
3470 2531 756
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2348 2380 3074 3106 3676
Size: 32 135 32 776 32 694 32 570
Cuts at: 3676 3708 3953 3985 5398 5430 5472 5504 1373
Size: 32 245 32 1413 32 42 32 2626
Fragments arranged by size:
2626 1413 776 694 570 245 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2383 3472 4101 4347 669
Size: 988 281 445 1089 629 246 3079
Fragments arranged by size:
3079 1089 988 629 445 281 246
HindIII A'AGCT_T
Cuts at: 748 2564 748
Size: 1816 4941
HpaI GTT'AAC
Cuts at: 4347 4347
Size: 6757
KpnI G_GTAC'C
Cuts at: 3804 4082 3804
Size: 278 6479
MfeI C'AATT_G
Cuts at: 4356 4356
Size: 6757
MluI A'CGCG_T
Cuts at: 6633 6633
Size: 6757
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2472 5871 6055 1305
Size: 1167 3399 184 2007
Fragments arranged by size:
3399 2007 1167 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4935
Fragments arranged by size:
4935 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2207 3290 4715 5074 5233 518
Size: 648 1041 1083 1425 359 159 2042
Fragments arranged by size:
2042 1425 1083 1041 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2226 2276 2461 2668 3161 4173 4850 5998
Size: 620 50 185 207 493 1012 677 1148
Cuts at: 5998 6243 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 1012 677 620 493 245 207
185 50
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6205
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6757
NheI G'CTAG_C
Cuts at: 1052 2269 1052
Size: 1217 5540
NotI GC'GGCC_GC
Cuts at: 4204 4204
Size: 6757
NspI r_CATG'y
Cuts at: 1132 1229 2302 2317 2455 3487 3561 4115 5660
Size: 97 1073 15 138 1032 74 554 1545
Cuts at: 5660 1132
Size: 2229
Fragments arranged by size:
2229 1545 1073 1032 554 138 97 74
15
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6757
PciI A'CATG_T
Cuts at: 5656 5656
Size: 6757
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2671 3016 1108
Size: 1563 345 4849
Fragments arranged by size:
4849 1563 345
PmeI GTTT'AAAC
Cuts at: 4061 4061
Size: 6757
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4826 4858 6339 6371 4826
Size: 32 1481 32 5212
Fragments arranged by size:
5212 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2768 2768
Size: 6757
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6757
PsiI TTA'TAA
Cuts at: 4327 4327
Size: 6757
PspOMI G'GGCC_C
Cuts at: 2895 2895
Size: 6757
PstI C_TGCA'G
Cuts at: 830 2146 2782 2904 2995 4109 830
Size: 1316 636 122 91 1114 3478
Fragments arranged by size:
3478 1316 1114 636 122 91
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6757
PvuII CAG'CTG
Cuts at: 2276 2461 2668 3161 2276
Size: 185 207 493 5872
Fragments arranged by size:
5872 493 207 185
SalI G'TCGA_C
Cuts at: 1936 2381 4099 1936
Size: 445 1718 4594
Fragments arranged by size:
4594 1718 445
SanDI GG'GwC_CC
Cuts at: 2768 2768
Size: 6757
SbfI CC_TGCA'GG
Cuts at: 4109 4109
Size: 6757
ScaI AGT'ACT
Cuts at: 2873 3928 5005 6717 2873
Size: 1055 1077 1712 2913
Fragments arranged by size:
2913 1712 1077 1055
SexAI A'CCwGG_T
Cuts at: 3877 3877
Size: 6757
SfcI C'TryA_G
Cuts at: 826 1045 2142 2456 2778 2900 2991 3373 4105
Size: 219 1097 314 322 122 91 382 732
Cuts at: 4105 5240 5921 6112 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1097 732 681 382 322 314
219 191 122 91
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6757
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6757
SmaI CCC'GGG
Cuts at: 1872 2015 4084 1872
Size: 143 2069 4545
Fragments arranged by size:
4545 2069 143
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2162 2534 4861 5762 6024 820
Size: 197 926 219 372 2327 901 262 1553
Fragments arranged by size:
2327 1553 926 901 372 262 219 197
SnaBI TAC'GTA
Cuts at: 493 4548 493
Size: 4055 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6757
SphI G_CATG'C
Cuts at: 1132 3487 4115 1132
Size: 2355 628 3774
Fragments arranged by size:
3774 2355 628
SspI AAT'ATT
Cuts at: 5 52 3652 4681 5
Size: 47 3600 1029 2081
Fragments arranged by size:
3600 2081 1029 47
StuI AGG'CCT
Cuts at: 1517 3813 1517
Size: 2296 4461
StyI C'CwwG_G
Cuts at: 513 1065 3030 4196 513
Size: 552 1965 1166 3074
Fragments arranged by size:
3074 1965 1166 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3211 3997 4784 4801 4954 5139 5584
Size: 1255 1631 786 787 17 153 185 445
Cuts at: 5584 325
Size: 1498
Fragments arranged by size:
1631 1498 1255 787 786 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2871 2925 3926
Size: 274 80 33 51 1443 894 54 1001
Cuts at: 3926 5003 6715 96
Size: 1077 1712 138
Fragments arranged by size:
1712 1443 1077 1001 894 274 138 80
54 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3770 3802 6717 6749 3770
Size: 32 2915 32 3778
Fragments arranged by size:
3778 2915 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6757
XbaI T'CTAG_A
Cuts at: 4093 4093
Size: 6757
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2350 2350
Size: 6757
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6757
XmaI C'CCGG_G
Cuts at: 1870 2013 4082 1870
Size: 143 2069 4545
Fragments arranged by size:
4545 2069 143
XmnI GAAnn'nnTTC
Cuts at: 2623 4886 2623
Size: 2263 4494
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2023 2504 4565 276
Size: 53 83 186 936 489 481 2061 2468
Fragments arranged by size:
2468 2061 936 489 481 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PspXI PsrI RsrII SacI SacII
SapI SfiI SfoI SrfI SwaI