Restriction map for
pFN21AB9620
(Circular) (Six-base) MAPSORT of: pFN21AB9620.seq Check: 2724 from: 1 to: 5746
pFN21AB9620 5746 bp
With 182 enzymes: SgfI *
March 28, 2012 14:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2164 2953 2164
Size: 789 4957
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3556 278
Size: 53 83 186 936 2020 2468
Fragments arranged by size:
2468 2020 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2583 3089 1937
Size: 646 506 4594
Fragments arranged by size:
4594 646 506
Acc65I G'GTAC_C
Cuts at: 3067 3067
Size: 5746
AclI AA'CG_TT
Cuts at: 1563 3873 4246 1563
Size: 2310 373 3063
Fragments arranged by size:
3063 2310 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2312 3809 5193 5461 1632
Size: 680 1497 1384 268 1917
Fragments arranged by size:
1917 1497 1384 680 268
AfeI AGC'GCT
Cuts at: 2187 2187
Size: 5746
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5549
AflIII A'CryG_T
Cuts at: 1203 4645 5622 1203
Size: 3442 977 1327
Fragments arranged by size:
3442 1327 977
AgeI A'CCGG_T
Cuts at: 4577 4577
Size: 5746
AhdI GACnn_n'nnGTC
Cuts at: 4475 4475
Size: 5746
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5746
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2846 1184
Size: 1662 4084
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2393 2425 2393
Size: 32 5714
AlwNI CAG_nnn'CTG
Cuts at: 3009 3019 3489 5061 3009
Size: 10 470 1572 3694
Fragments arranged by size:
3694 1572 470 10
ApaI G_GGCC'C
Cuts at: 2511 2511
Size: 5746
ApaLI G'TGCA_C
Cuts at: 3803 4959 3803
Size: 1156 4590
ApoI r'AATT_y
Cuts at: 1493 3055 3281 3433 5542 1493
Size: 1562 226 152 2109 1697
Fragments arranged by size:
2109 1697 1562 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4300 160
Size: 872 3268 1606
Fragments arranged by size:
3268 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5746
AvaI C'yCGr_G
Cuts at: 1870 1943 2086 2102 2306 2312 2512 3071 1870
Size: 73 143 16 204 6 200 559 4545
Fragments arranged by size:
4545 559 204 200 143 73 16 6
BamHI G'GATC_C
Cuts at: 3076 3443 3076
Size: 367 5379
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2054 2107 2234 2248 2612
Size: 325 217 627 267 53 127 14 364
Cuts at: 2612 2630 2740 3034 3067 4522 618
Size: 18 110 294 33 1455 1842
Fragments arranged by size:
1842 1455 627 364 325 294 267 217
127 110 53 33 18 14
BanII G_rGCy'C
Cuts at: 1391 2313 2366 2511 2531 2771 3065 1391
Size: 922 53 145 20 240 294 4072
Fragments arranged by size:
4072 922 294 240 145 53 20
BbeI G_GCGC'C
Cuts at: 2058 2111 2238 2616 2634 2058
Size: 53 127 378 18 5170
Fragments arranged by size:
5170 378 127 53 18
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2912 928
Size: 971 1013 3762
Fragments arranged by size:
3762 1013 971
BbvCI CC'TCA_GC
Cuts at: 2990 2990
Size: 5746
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2824 2858 2935 2969 3937 3971 1903
Size: 34 887 34 77 34 968 34 3678
Fragments arranged by size:
3678 968 887 77 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3638 4848 3638
Size: 1210 4536
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5746
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2049 2558 4357
Size: 107 122 71 631 605 377 509 1799
Cuts at: 4357 136
Size: 1525
Fragments arranged by size:
1799 1525 631 605 509 377 122 107
71
BglII A'GATC_T
Cuts at: 5741 5741
Size: 5746
BlpI GC'TnA_GC
Cuts at: 2036 2159 3163 2036
Size: 123 1004 4619
Fragments arranged by size:
4619 1004 123
Bme1580I G_kGCm'C
Cuts at: 1790 2511 2519 2667 2745 3039 3807 4963 1790
Size: 721 8 148 78 294 768 1156 2573
Fragments arranged by size:
2573 1156 768 721 294 148 78 8
BmgBI CAC'GTC
Cuts at: 1318 2749 2998 1318
Size: 1431 249 4066
Fragments arranged by size:
4066 1431 249
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3477 4435 442
Size: 416 53 83 399 24 2060 958 1753
Fragments arranged by size:
2060 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5746
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2899 2899
Size: 5746
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2456 2894 4406 1239
Size: 1217 438 1512 2579
Fragments arranged by size:
2579 1512 1217 438
Bpu10I CC'TnA_GC
Cuts at: 2594 2990 2594
Size: 396 5350
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2174 2705 2897 2960 3871 4736 5034 2174
Size: 531 192 63 911 865 298 2886
Fragments arranged by size:
2886 911 865 531 298 192 63
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4409 882
Size: 617 2910 2219
Fragments arranged by size:
2910 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3537 493
Size: 711 2333 2702
Fragments arranged by size:
2702 2333 711
BsaBI GATnn'nnATC
Cuts at: 5740 5740
Size: 5746
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2055 2108 2235 2613
Size: 53 83 186 936 522 53 127 378
Cuts at: 2613 2631 2936 3553 3935 4570 275
Size: 18 305 617 382 635 1451
Fragments arranged by size:
1451 936 635 617 522 382 378 305
186 127 83 53 53 18
BsaWI w'CCGG_w
Cuts at: 4179 4577 4851 4998 5409 4179
Size: 398 274 147 411 4516
Fragments arranged by size:
4516 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2393 2423 2921 2951 1178
Size: 30 165 30 990 30 498 30 3973
Fragments arranged by size:
3973 990 498 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2414 1186
Size: 1228 4518
BseYI C'CCAG_C
Cuts at: 2240 3024 4949 5445 2240
Size: 784 1925 496 2541
Fragments arranged by size:
2541 1925 784 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5173
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2121 2127 2202 2478 2565 2643
Size: 275 186 120 6 75 276 87 78
Cuts at: 2643 3196 3957 4985 1540
Size: 553 761 1028 2301
Fragments arranged by size:
2301 1028 761 553 276 275 186 120
87 78 75 6
BsiHKAI G_wGCw'C
Cuts at: 2313 2366 3807 3892 4963 2313
Size: 53 1441 85 1071 3096
Fragments arranged by size:
3096 1441 1071 85 53
BsiWI C'GTAC_G
Cuts at: 3042 3042
Size: 5746
BsmI GAATG_Cn'
Cuts at: 3257 3350 3257
Size: 93 5653
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2313 2366 2511 2519 2531 2667 2745
Size: 399 523 53 145 8 12 136 78
Cuts at: 2745 2771 3039 3065 3807 3892 4963 1391
Size: 26 268 26 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 523 399 268 145 136
85 78 53 26 26 12 8
BspEI T'CCGG_A
Cuts at: 5409 5409
Size: 5746
BspHI T'CATG_A
Cuts at: 3635 5365 3635
Size: 1730 4016
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2164 2953 3101 844
Size: 1320 789 148 3489
Fragments arranged by size:
3489 1320 789 148
BsrBI CCG'CTC
Cuts at: 1394 1823 2040 3633 1394
Size: 429 217 1593 3507
Fragments arranged by size:
3507 1593 429 217
BsrDI GCAATG_nn'
Cuts at: 65 1248 4241 4415 65
Size: 1183 2993 174 1396
Fragments arranged by size:
2993 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2126 4390 4577 1700
Size: 110 316 2264 187 2869
Fragments arranged by size:
2869 2264 316 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5746
BssHII G'CGCG_C
Cuts at: 1925 2224 1925
Size: 299 5447
BssSI C'ACGA_G
Cuts at: 3806 4818 3806
Size: 1012 4734
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3034 794
Size: 2240 3506
BstBI TT'CG_AA
Cuts at: 4550 4550
Size: 5746
BstEII G'GTnAC_C
Cuts at: 1184 2344 1184
Size: 1160 4586
BstXI CCAn_nnnn'nTGG
Cuts at: 2341 2791 2341
Size: 450 5296
BstYI r'GATC_y
Cuts at: 1972 3076 3443 3828 3845 5297 5383 5395 5741
Size: 1104 367 385 17 1452 86 12 346
Cuts at: 5741 1972
Size: 1977
Fragments arranged by size:
1977 1452 1104 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2121 2523 2940 513
Size: 552 939 117 402 417 3319
Fragments arranged by size:
3319 939 552 417 402 117
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2040 2827 487
Size: 683 56 814 787 3406
Fragments arranged by size:
3406 814 787 683 56
BtsI GCAGTG_nn'
Cuts at: 3262 4056 4076 3262
Size: 794 20 4932
Fragments arranged by size:
4932 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5746
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5711
DraI TTT'AAA
Cuts at: 3050 3050
Size: 5746
DraIII CAC_nnn'GTG
Cuts at: 3034 3034
Size: 5746
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4753 809
Size: 507 3437 1802
Fragments arranged by size:
3437 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2118 2124 2199
Size: 54 1425 50 275 18 288 6 75
Cuts at: 2199 2391 2475 2520 2562 3193 4082 5711 8
Size: 192 84 45 42 631 889 1629 43
Fragments arranged by size:
1629 1425 889 631 288 275 192 84
75 54 50 45 43 42 18 6
EagI C'GGCC_G
Cuts at: 1537 1812 2118 2124 2199 2475 2562 3193 1537
Size: 275 306 6 75 276 87 631 4090
Fragments arranged by size:
4090 631 306 276 275 87 75 6
EarI CTCTTCn'nnn_
Cuts at: 1353 2889 3676 1353
Size: 1536 787 3423
Fragments arranged by size:
3423 1536 787
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2831 4333 4707 4853 387
Size: 1064 138 1242 1502 374 146 1280
Fragments arranged by size:
1502 1280 1242 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2311 2364 2311
Size: 53 5693
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2312 2456 2894 3809 4406 5193 5461
Size: 393 680 144 438 915 597 787 268
Cuts at: 5461 1239
Size: 1524
Fragments arranged by size:
1524 915 787 680 597 438 393 268
144
EcoO109I rG'GnC_Cy
Cuts at: 2431 2507 2508 2431
Size: 76 1 5669
Fragments arranged by size:
5669 76 1
EcoRI G'AATT_C
Cuts at: 3055 3433 3055
Size: 378 5368
FspI TGC'GCA
Cuts at: 2834 4252 2834
Size: 1418 4328
HaeII r_GCGC'y
Cuts at: 1678 2036 2058 2111 2189 2238 2320 2579 2616
Size: 358 22 53 78 49 82 259 37
Cuts at: 2616 2634 4893 1678
Size: 18 2259 2531
Fragments arranged by size:
2531 2259 358 259 82 78 53 49
37 22 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2921 2953 4387 4419 4461
Size: 32 135 32 1349 32 1434 32 42
Cuts at: 4461 4493 1373
Size: 32 2626
Fragments arranged by size:
2626 1434 1349 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3001 3090 3336 669
Size: 988 281 1063 89 246 3079
Fragments arranged by size:
3079 1063 988 281 246 89
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5746
HpaI GTT'AAC
Cuts at: 3336 3336
Size: 5746
KasI G'GCGC_C
Cuts at: 2054 2107 2234 2612 2630 2054
Size: 53 127 378 18 5170
Fragments arranged by size:
5170 378 127 53 18
KpnI G_GTAC'C
Cuts at: 3071 3071
Size: 5746
MfeI C'AATT_G
Cuts at: 3345 3345
Size: 5746
MluI A'CGCG_T
Cuts at: 5622 5622
Size: 5746
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2358 4860 5044 1305
Size: 1053 2502 184 2007
Fragments arranged by size:
2502 2007 1053 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3924
Fragments arranged by size:
3924 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2789 3704 4063 4222 518
Size: 648 1623 915 359 159 2042
Fragments arranged by size:
2042 1623 915 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2067 2123 2480 3006 3016 3162 3839 4987
Size: 461 56 357 526 10 146 677 1148
Cuts at: 4987 5232 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 526 461 357 245 146
56 10
NaeI GCC'GGC
Cuts at: 2128 2128
Size: 5746
NarI GG'CG_CC
Cuts at: 2055 2108 2235 2613 2631 2055
Size: 53 127 378 18 5170
Fragments arranged by size:
5170 378 127 53 18
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4255
Fragments arranged by size:
4255 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5746
NgoMIV G'CCGG_C
Cuts at: 2126 2126
Size: 5746
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5746
NotI GC'GGCC_GC
Cuts at: 2199 2475 2562 3193 2199
Size: 276 87 631 4752
Fragments arranged by size:
4752 631 276 87
NspI r_CATG'y
Cuts at: 1132 1229 3104 4649 1132
Size: 97 1875 1545 2229
Fragments arranged by size:
2229 1875 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5746
PciI A'CATG_T
Cuts at: 4645 4645
Size: 5746
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5746
PmeI GTTT'AAAC
Cuts at: 3050 3050
Size: 5746
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2393 2425 3815 3847 5328 5360 2393
Size: 32 1390 32 1481 32 2779
Fragments arranged by size:
2779 1481 1390 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5746
PsiI TTA'TAA
Cuts at: 3316 3316
Size: 5746
PspOMI G'GGCC_C
Cuts at: 2507 2507
Size: 5746
PspXI vC'TCGA_Gb
Cuts at: 2312 2312
Size: 5746
PstI C_TGCA'G
Cuts at: 830 3098 830
Size: 2268 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5746
PvuII CAG'CTG
Cuts at: 3016 3016
Size: 5746
SacI G_AGCT'C
Cuts at: 2313 2366 2313
Size: 53 5693
SacII CC_GC'GG
Cuts at: 2124 2124
Size: 5746
SalI G'TCGA_C
Cuts at: 1936 3088 1936
Size: 1152 4594
SbfI CC_TGCA'GG
Cuts at: 3098 3098
Size: 5746
ScaI AGT'ACT
Cuts at: 3994 5706 3994
Size: 1712 4034
SexAI A'CCwGG_T
Cuts at: 3030 3030
Size: 5746
SfcI C'TryA_G
Cuts at: 826 1045 3094 4229 4910 5101 826
Size: 219 2049 1135 681 191 1471
Fragments arranged by size:
2049 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2056 2109 2236 2614 2632 2056
Size: 53 127 378 18 5170
Fragments arranged by size:
5170 378 127 53 18
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5746
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5746
SmaI CCC'GGG
Cuts at: 1872 2088 2104 3073 1872
Size: 216 16 969 4545
Fragments arranged by size:
4545 969 216 16
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2189 2312 2720 2912 2975 3850
Size: 197 926 246 123 408 192 63 875
Cuts at: 3850 4751 5013 820
Size: 901 262 1553
Fragments arranged by size:
1553 926 901 875 408 262 246 197
192 123 63
SnaBI TAC'GTA
Cuts at: 493 3537 493
Size: 3044 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5746
SphI G_CATG'C
Cuts at: 1132 3104 1132
Size: 1972 3774
SspI AAT'ATT
Cuts at: 5 52 3670 5
Size: 47 3618 2081
Fragments arranged by size:
3618 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5746
StyI C'CwwG_G
Cuts at: 513 1065 2004 2382 3185 513
Size: 552 939 378 803 3074
Fragments arranged by size:
3074 939 803 552 378
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3773 3790 3943 4128 4573 325
Size: 1255 2193 17 153 185 445 1498
Fragments arranged by size:
2193 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3992 5704 96
Size: 274 80 33 51 1443 2015 1712 138
Fragments arranged by size:
2015 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5706 5738 5706
Size: 32 5714
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5746
XbaI T'CTAG_A
Cuts at: 3082 3082
Size: 5746
XhoI C'TCGA_G
Cuts at: 1943 2312 1943
Size: 369 5377
XmaI C'CCGG_G
Cuts at: 1870 2086 2102 3071 1870
Size: 216 16 969 4545
Fragments arranged by size:
4545 969 216 16
XmnI GAAnn'nnTTC
Cuts at: 3875 3875
Size: 5746
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3554 276
Size: 53 83 186 936 2020 2468
Fragments arranged by size:
2468 2020 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI
SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BfrBI BsmBI BstZ17I Bsu36I EcoNI
EcoRV FalI FseI FspAI NruI NsiI PacI PfoI
PmlI PpuMI PsrI RsrII SanDI SapI SfiI SrfI
SwaI XcmI