Restriction map for
pFN21AB9611
(Circular) (Six-base) MAPSORT of: pFN21AB9611.seq Check: 6059 from: 1 to: 6472
pFN21AB9611 6472 bp
With 182 enzymes: SgfI *
October 20, 2009 10:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3771 3771
Size: 6472
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2148 4282 278
Size: 53 83 186 936 612 2134 2468
Fragments arranged by size:
2468 2134 936 612 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3815 1937
Size: 1878 4594
Acc65I G'GTAC_C
Cuts at: 3793 3793
Size: 6472
AclI AA'CG_TT
Cuts at: 1563 4599 4972 1563
Size: 3036 373 3063
Fragments arranged by size:
3063 3036 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3356 3672 4535 5919 6187 1632
Size: 1724 316 863 1384 268 1917
Fragments arranged by size:
1917 1724 1384 863 316 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6275
AflIII A'CryG_T
Cuts at: 1203 5371 6348 1203
Size: 4168 977 1327
Fragments arranged by size:
4168 1327 977
AgeI A'CCGG_T
Cuts at: 5303 5303
Size: 6472
AhdI GACnn_n'nnGTC
Cuts at: 3379 5201 3379
Size: 1822 4650
AleI CACnn'nnGTG
Cuts at: 1166 3713 1166
Size: 2547 3925
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2480 1184
Size: 1296 5176
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3050 3082 3050
Size: 32 6440
AlwNI CAG_nnn'CTG
Cuts at: 2862 3464 3753 4215 5787 2862
Size: 602 289 462 1572 3547
Fragments arranged by size:
3547 1572 602 462 289
ApaLI G'TGCA_C
Cuts at: 2525 4529 5685 2525
Size: 2004 1156 3312
Fragments arranged by size:
3312 2004 1156
ApoI r'AATT_y
Cuts at: 1493 3509 3781 4007 4159 6268 1493
Size: 2016 272 226 152 2109 1697
Fragments arranged by size:
2109 2016 1697 272 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5026 160
Size: 872 3994 1606
Fragments arranged by size:
3994 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6472
AvaI C'yCGr_G
Cuts at: 1870 1943 2596 2693 3797 1870
Size: 73 653 97 1104 4545
Fragments arranged by size:
4545 1104 653 97 73
BamHI G'GATC_C
Cuts at: 2829 3802 4169 2829
Size: 973 367 5132
Fragments arranged by size:
5132 973 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3118 3793 5248 618
Size: 325 217 627 1331 675 1455 1842
Fragments arranged by size:
1842 1455 1331 675 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2700 3053 3791 1391
Size: 1309 353 738 4072
Fragments arranged by size:
4072 1309 738 353
BbeI G_GCGC'C
Cuts at: 3122 3122
Size: 6472
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2626 3202 928
Size: 971 727 576 4198
Fragments arranged by size:
4198 971 727 576
BbvCI CC'TCA_GC
Cuts at: 3010 3010
Size: 6472
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4663 4697 1903
Size: 34 2726 34 3678
Fragments arranged by size:
3678 2726 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4364 5574 4364
Size: 1210 5262
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6472
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2195 3524 5083
Size: 107 122 71 631 605 523 1329 1559
Cuts at: 5083 136
Size: 1525
Fragments arranged by size:
1559 1525 1329 631 605 523 122 107
71
BglII A'GATC_T
Cuts at: 2931 3483 6467 2931
Size: 552 2984 2936
Fragments arranged by size:
2984 2936 552
BlpI GC'TnA_GC
Cuts at: 3151 3156 3889 3151
Size: 5 733 5734
Fragments arranged by size:
5734 733 5
Bme1580I G_kGCm'C
Cuts at: 1790 2529 2869 3440 3720 4533 5689 1790
Size: 739 340 571 280 813 1156 2573
Fragments arranged by size:
2573 1156 813 739 571 340 280
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6472
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4203 5161 442
Size: 416 53 83 399 24 2786 958 1753
Fragments arranged by size:
2786 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6472
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2770 2770
Size: 6472
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2318 2444 2630 2633 2690 2740 3182 5132
Size: 1079 126 186 3 57 50 442 1950
Cuts at: 5132 1239
Size: 2579
Fragments arranged by size:
2579 1950 1079 442 186 126 57 50
3
Bpu10I CC'TnA_GC
Cuts at: 3010 3010
Size: 6472
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4597 5462 5760 4597
Size: 865 298 5309
Fragments arranged by size:
5309 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2018 2606 3070 3539 5135 882
Size: 617 519 588 464 469 1596 2219
Fragments arranged by size:
2219 1596 617 588 519 469 464
BsaAI yAC'GTr
Cuts at: 493 1204 4263 493
Size: 711 3059 2702
Fragments arranged by size:
3059 2702 711
BsaBI GATnn'nnATC
Cuts at: 3488 6466 3488
Size: 2978 3494
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2145 2475 3119 3303
Size: 53 83 186 936 612 330 644 184
Cuts at: 3303 4279 4661 5296 275
Size: 976 382 635 1451
Fragments arranged by size:
1451 976 936 644 635 612 382 330
186 184 83 53
BsaWI w'CCGG_w
Cuts at: 4905 5303 5577 5724 6135 4905
Size: 398 274 147 411 5242
Fragments arranged by size:
5242 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2066 2096 3050 3080 3401
Size: 30 165 30 663 30 954 30 321
Cuts at: 3401 3431 1178
Size: 30 4219
Fragments arranged by size:
4219 954 663 321 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2314 2360 2690 2759 1186
Size: 1128 46 330 69 4899
Fragments arranged by size:
4899 1128 330 69 46
BseYI C'CCAG_C
Cuts at: 2984 3441 5675 6171 2984
Size: 457 2234 496 3285
Fragments arranged by size:
3285 2234 496 457
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2267 1163
Size: 573 531 5368
Fragments arranged by size:
5368 573 531
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2167 3018 3922 4683 5711 1540
Size: 275 186 166 851 904 761 1028 2301
Fragments arranged by size:
2301 1028 904 851 761 275 186 166
BsiHKAI G_wGCw'C
Cuts at: 2529 2700 4533 4618 5689 2529
Size: 171 1833 85 1071 3312
Fragments arranged by size:
3312 1833 1071 171 85
BsmI GAATG_Cn'
Cuts at: 3983 4076 3983
Size: 93 6379
BsmBI CGTCTCn'nnnn_
Cuts at: 2528 2758 2528
Size: 230 6242
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2529 2700 2869 3053 3440 3720 3791
Size: 399 739 171 169 184 387 280 71
Cuts at: 3791 4533 4618 5689 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 739 399 387 280 184
171 169 85 71
BspEI T'CCGG_A
Cuts at: 6135 6135
Size: 6472
BspHI T'CATG_A
Cuts at: 4361 6091 4361
Size: 1730 4742
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2289 2988 3771 3827 844
Size: 1445 699 783 56 3489
Fragments arranged by size:
3489 1445 783 699 56
BsrBI CCG'CTC
Cuts at: 1394 1823 2626 4359 1394
Size: 429 803 1733 3507
Fragments arranged by size:
3507 1733 803 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3316 4967 5141 65
Size: 1183 2068 1651 174 1396
Fragments arranged by size:
2068 1651 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2490 5116 5303 1700
Size: 110 680 2626 187 2869
Fragments arranged by size:
2869 2626 680 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6472
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6472
BssSI C'ACGA_G
Cuts at: 4532 5544 4532
Size: 1012 5460
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2130 3594 794
Size: 1336 1464 3672
Fragments arranged by size:
3672 1464 1336
BstBI TT'CG_AA
Cuts at: 5276 5276
Size: 6472
BstEII G'GTnAC_C
Cuts at: 1184 2851 1184
Size: 1667 4805
BstXI CCAn_nnnn'nTGG
Cuts at: 2452 2452
Size: 6472
BstYI r'GATC_y
Cuts at: 1972 2829 2931 3483 3802 4169 4554 4571 6023
Size: 857 102 552 319 367 385 17 1452
Cuts at: 6023 6109 6121 6467 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 857 552 385 367 346 319
102 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2233 2675 3145 3735 3768 513
Size: 552 1168 442 470 590 33 3217
Fragments arranged by size:
3217 1168 590 552 470 442 33
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2317 487
Size: 683 56 1091 4642
Fragments arranged by size:
4642 1091 683 56
BtsI GCAGTG_nn'
Cuts at: 2128 2579 3324 3988 4782 4802 2128
Size: 451 745 664 794 20 3798
Fragments arranged by size:
3798 794 745 664 451 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6472
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3724 3759 529
Size: 35 3160 35 3242
Fragments arranged by size:
3242 3160 35 35
DraI TTT'AAA
Cuts at: 3776 3776
Size: 6472
DraIII CAC_nnn'GTG
Cuts at: 2398 2398
Size: 6472
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2032 5479 809
Size: 507 716 3447 1802
Fragments arranged by size:
3447 1802 716 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2196 2870 3142
Size: 54 1425 50 275 18 366 674 272
Cuts at: 3142 3919 4808 6437 8
Size: 777 889 1629 43
Fragments arranged by size:
1629 1425 889 777 674 366 275 272
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3919 1537
Size: 275 2107 4090
Fragments arranged by size:
4090 2107 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2075 4402 1353
Size: 722 2327 3423
Fragments arranged by size:
3423 2327 722
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5059 5433 5579 387
Size: 1064 138 3470 374 146 1280
Fragments arranged by size:
3470 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2698 2698
Size: 6472
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2318 2444 2630 2633 2690 2740 3182
Size: 393 686 126 186 3 57 50 442
Cuts at: 3182 3356 3672 4535 5132 5919 6187 1239
Size: 174 316 863 597 787 268 1524
Fragments arranged by size:
1524 863 787 686 597 442 393 316
268 186 174 126 57 50 3
EcoNI CCTnn'n_nnAGG
Cuts at: 2570 2744 2570
Size: 174 6298
EcoO109I rG'GnC_Cy
Cuts at: 2764 2809 3648 2764
Size: 45 839 5588
Fragments arranged by size:
5588 839 45
EcoRI G'AATT_C
Cuts at: 3509 3781 4159 3509
Size: 272 378 5822
Fragments arranged by size:
5822 378 272
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2704 2704
Size: 6472
FspI TGC'GCA
Cuts at: 4978 4978
Size: 6472
HaeII r_GCGC'y
Cuts at: 1678 3009 3122 5619 1678
Size: 1331 113 2497 2531
Fragments arranged by size:
2531 2497 1331 113
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2579 2611 3092 3124 3365
Size: 32 135 32 1007 32 481 32 241
Cuts at: 3365 3397 5113 5145 5187 5219 1373
Size: 32 1716 32 42 32 2626
Fragments arranged by size:
2626 1716 1007 481 241 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2266 3816 4062 669
Size: 988 281 328 1550 246 3079
Fragments arranged by size:
3079 1550 988 328 281 246
HindIII A'AGCT_T
Cuts at: 748 2792 748
Size: 2044 4428
HpaI GTT'AAC
Cuts at: 4062 4062
Size: 6472
KasI G'GCGC_C
Cuts at: 3118 3118
Size: 6472
KpnI G_GTAC'C
Cuts at: 3797 3797
Size: 6472
MfeI C'AATT_G
Cuts at: 4071 4071
Size: 6472
MluI A'CGCG_T
Cuts at: 6348 6348
Size: 6472
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2346 2469 2757 5586 5770 1305
Size: 1041 123 288 2829 184 2007
Fragments arranged by size:
2829 2007 1041 288 184 123
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3144 10
Size: 54 1425 343 1312 3338
Fragments arranged by size:
3338 1425 1312 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3713 4430 4789 4948 518
Size: 648 2547 717 359 159 2042
Fragments arranged by size:
2547 2042 717 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3155 3464 3770 3888 4565 5713 5958 1606
Size: 1549 309 306 118 677 1148 245 2120
Fragments arranged by size:
2120 1549 1148 677 309 306 245 118
NaeI GCC'GGC
Cuts at: 2492 2492
Size: 6472
NarI GG'CG_CC
Cuts at: 3119 3119
Size: 6472
NcoI C'CATG_G
Cuts at: 513 1065 2233 3145 513
Size: 552 1168 912 3840
Fragments arranged by size:
3840 1168 912 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6472
NgoMIV G'CCGG_C
Cuts at: 2490 2490
Size: 6472
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6472
NotI GC'GGCC_GC
Cuts at: 3919 3919
Size: 6472
NspI r_CATG'y
Cuts at: 1132 1229 2137 2383 2518 3592 3830 5375 1132
Size: 97 908 246 135 1074 238 1545 2229
Fragments arranged by size:
2229 1545 1074 908 246 238 135 97
PasI CC'CwG_GG
Cuts at: 1348 3260 1348
Size: 1912 4560
PciI A'CATG_T
Cuts at: 5371 5371
Size: 6472
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3273 3555 3604 1108
Size: 2165 282 49 3976
Fragments arranged by size:
3976 2165 282 49
PfoI T'CCnGG_A
Cuts at: 2271 2421 2271
Size: 150 6322
PmeI GTTT'AAAC
Cuts at: 3776 3776
Size: 6472
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2210 2242 3050 3082 4541 4573 6054 6086 2210
Size: 32 808 32 1459 32 1481 32 2596
Fragments arranged by size:
2596 1481 1459 808 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3648 3648
Size: 6472
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6472
PsiI TTA'TAA
Cuts at: 4042 4042
Size: 6472
PstI C_TGCA'G
Cuts at: 830 2388 2745 2838 3824 830
Size: 1558 357 93 986 3478
Fragments arranged by size:
3478 1558 986 357 93
PvuI CG_AT'CG
Cuts at: 2001 3018 2001
Size: 1017 5455
PvuII CAG'CTG
Cuts at: 3155 3155
Size: 6472
SacI G_AGCT'C
Cuts at: 2700 2700
Size: 6472
SacII CC_GC'GG
Cuts at: 3771 3771
Size: 6472
SalI G'TCGA_C
Cuts at: 1936 3814 1936
Size: 1878 4594
SanDI GG'GwC_CC
Cuts at: 3648 3648
Size: 6472
SbfI CC_TGCA'GG
Cuts at: 2745 3824 2745
Size: 1079 5393
ScaI AGT'ACT
Cuts at: 4720 6432 4720
Size: 1712 4760
SexAI A'CCwGG_T
Cuts at: 2937 2937
Size: 6472
SfcI C'TryA_G
Cuts at: 826 1045 2384 2741 2834 3820 4955 5636 5827
Size: 219 1339 357 93 986 1135 681 191
Cuts at: 5827 826
Size: 1471
Fragments arranged by size:
1471 1339 1135 986 681 357 219 191
93
SfoI GGC'GCC
Cuts at: 3120 3120
Size: 6472
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6472
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6472
SmaI CCC'GGG
Cuts at: 1872 3799 1872
Size: 1927 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2596 4576 5477 5739 820
Size: 197 926 653 1980 901 262 1553
Fragments arranged by size:
1980 1553 926 901 653 262 197
SnaBI TAC'GTA
Cuts at: 493 4263 493
Size: 3770 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6472
SphI G_CATG'C
Cuts at: 1132 3830 1132
Size: 2698 3774
SspI AAT'ATT
Cuts at: 5 52 4396 5
Size: 47 4344 2081
Fragments arranged by size:
4344 2081 47
StuI AGG'CCT
Cuts at: 1517 2081 1517
Size: 564 5908
StyI C'CwwG_G
Cuts at: 513 1065 2233 2571 2955 2970 3069 3111 3145
Size: 552 1168 338 384 15 99 42 34
Cuts at: 3145 3384 3446 3911 513
Size: 239 62 465 3074
Fragments arranged by size:
3074 1168 552 465 384 338 239 99
62 42 34 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 1999 2630 2644 3070 4499 4516 4669
Size: 1255 419 631 14 426 1429 17 153
Cuts at: 4669 4854 5299 325
Size: 185 445 1498
Fragments arranged by size:
1498 1429 1255 631 445 426 419 185
153 17 14
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4718 6430 96
Size: 274 80 33 51 1443 2741 1712 138
Fragments arranged by size:
2741 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2066 2098 2667 2699 3401 3433 6432 6464 2066
Size: 32 569 32 702 32 2999 32 2074
Fragments arranged by size:
2999 2074 702 569 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2261 1316
Size: 945 5527
XbaI T'CTAG_A
Cuts at: 3808 3808
Size: 6472
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2797 2797
Size: 6472
XhoI C'TCGA_G
Cuts at: 1943 2596 1943
Size: 653 5819
XmaI C'CCGG_G
Cuts at: 1870 3797 1870
Size: 1927 4545
XmnI GAAnn'nnTTC
Cuts at: 2435 2999 4601 2435
Size: 564 1602 4306
Fragments arranged by size:
4306 1602 564
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2146 4280 276
Size: 53 83 186 936 612 2134 2468
Fragments arranged by size:
2468 2134 936 612 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SacII SalI SanDI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BfrBI BsiWI BstZ17I
Bsu36I EcoRV FseI FspAI NruI NsiI PacI PmlI
PspOMI PspXI PsrI RsrII SapI SfiI SrfI SwaI