Restriction map for
pFN21AB9610
(Circular) (Six-base) MAPSORT of: pFN21AB9610.seq Check: 2925 from: 1 to: 6274
pFN21AB9610 6274 bp
With 182 enzymes: SgfI *
April 15, 2010 09:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2224 2224
Size: 6274
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4084 278
Size: 53 83 186 936 2548 2468
Fragments arranged by size:
2548 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3617 1937
Size: 1680 4594
Acc65I G'GTAC_C
Cuts at: 3058 3595 3058
Size: 537 5737
AclI AA'CG_TT
Cuts at: 1563 4401 4774 1563
Size: 2838 373 3063
Fragments arranged by size:
3063 2838 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4337 5721 5989 1632
Size: 2705 1384 268 1917
Fragments arranged by size:
2705 1917 1384 268
AfeI AGC'GCT
Cuts at: 3117 3117
Size: 6274
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6077
AflIII A'CryG_T
Cuts at: 1203 5173 6150 1203
Size: 3970 977 1327
Fragments arranged by size:
3970 1327 977
AgeI A'CCGG_T
Cuts at: 5105 5105
Size: 6274
AhdI GACnn_n'nnGTC
Cuts at: 5003 5003
Size: 6274
AleI CACnn'nnGTG
Cuts at: 1166 3203 1166
Size: 2037 4237
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6274
AlwNI CAG_nnn'CTG
Cuts at: 2491 2671 3382 3397 3467 4017 5589 2491
Size: 180 711 15 70 550 1572 3176
Fragments arranged by size:
3176 1572 711 550 180 70 15
ApaI G_GGCC'C
Cuts at: 3151 3461 3565 3151
Size: 310 104 5860
Fragments arranged by size:
5860 310 104
ApaLI G'TGCA_C
Cuts at: 2130 4331 5487 2130
Size: 2201 1156 2917
Fragments arranged by size:
2917 2201 1156
ApoI r'AATT_y
Cuts at: 1493 2353 3583 3809 3961 6070 1493
Size: 860 1230 226 152 2109 1697
Fragments arranged by size:
2109 1697 1230 860 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4828 160
Size: 872 3796 1606
Fragments arranged by size:
3796 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6274
AvaI C'yCGr_G
Cuts at: 1870 1943 2709 3258 3599 1870
Size: 73 766 549 341 4545
Fragments arranged by size:
4545 766 549 341 73
BamHI G'GATC_C
Cuts at: 3604 3971 3604
Size: 367 5907
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2469 2647 3058 3192 3595
Size: 325 217 627 682 178 411 134 403
Cuts at: 3595 5050 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 682 627 411 403 325 217
178 134
BanII G_rGCy'C
Cuts at: 1391 2631 2694 3054 3151 3391 3461 3474 3521
Size: 1240 63 360 97 240 70 13 47
Cuts at: 3521 3528 3565 3593 1391
Size: 7 37 28 4072
Fragments arranged by size:
4072 1240 360 240 97 70 63 47
37 28 13 7
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5303
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4465 4499 1903
Size: 34 2528 34 3678
Fragments arranged by size:
3678 2528 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4166 5376 4166
Size: 1210 5064
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6274
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4885 136
Size: 107 122 71 631 605 3213 1525
Fragments arranged by size:
3213 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6269 6269
Size: 6274
BlpI GC'TnA_GC
Cuts at: 3077 3691 3077
Size: 614 5660
Bme1580I G_kGCm'C
Cuts at: 1790 2038 2134 2472 2652 3151 3440 3461 3565
Size: 248 96 338 180 499 289 21 104
Cuts at: 3565 3573 4335 5491 1790
Size: 8 762 1156 2573
Fragments arranged by size:
2573 1156 762 499 338 289 248 180
104 96 21 8
BmgBI CAC'GTC
Cuts at: 1318 3356 1318
Size: 2038 4236
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2923 3154 4005
Size: 416 53 83 399 24 1506 231 851
Cuts at: 4005 4963 442
Size: 958 1753
Fragments arranged by size:
1753 1506 958 851 416 399 231 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6274
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2213 2829 3389 3396 3512 4934 1239
Size: 974 616 560 7 116 1422 2579
Fragments arranged by size:
2579 1422 974 616 560 116 7
Bpu10I CC'TnA_GC
Cuts at: 3390 3468 3390
Size: 78 6196
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2750 4399 5264 5562 2750
Size: 1649 865 298 3462
Fragments arranged by size:
3462 1649 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2180 4937 882
Size: 617 681 2757 2219
Fragments arranged by size:
2757 2219 681 617
BsaAI yAC'GTr
Cuts at: 493 1204 4065 493
Size: 711 2861 2702
Fragments arranged by size:
2861 2702 711
BsaBI GATnn'nnATC
Cuts at: 6268 6268
Size: 6274
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2526 4081 4463 5098
Size: 53 83 186 936 993 1555 382 635
Cuts at: 5098 275
Size: 1451
Fragments arranged by size:
1555 1451 993 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4707 5105 5379 5526 5937 4707
Size: 398 274 147 411 5044
Fragments arranged by size:
5044 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6049
Fragments arranged by size:
6049 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3363 1186
Size: 2177 4097
BseYI C'CCAG_C
Cuts at: 3118 3290 3558 5477 5973 3118
Size: 172 268 1919 496 3419
Fragments arranged by size:
3419 1919 496 268 172
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2600 3309 3574 1163
Size: 573 864 709 265 3863
Fragments arranged by size:
3863 864 709 573 265
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3724 4485 5513 1540
Size: 275 186 1723 761 1028 2301
Fragments arranged by size:
2301 1723 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2134 2694 3083 3212 3391 4335 4420 5491 2134
Size: 560 389 129 179 944 85 1071 2917
Fragments arranged by size:
2917 1071 944 560 389 179 129 85
BsmI GAATG_Cn'
Cuts at: 3154 3785 3878 3154
Size: 631 93 5550
Fragments arranged by size:
5550 631 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2038 2134 2472 2631 2652 2694 3054
Size: 399 248 96 338 159 21 42 360
Cuts at: 3054 3083 3151 3212 3391 3440 3461 3474 3521
Size: 29 68 61 179 49 21 13 47
Cuts at: 3521 3528 3565 3573 3593 4335 4420 5491 1391
Size: 7 37 8 20 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 360 338 248 179
159 96 85 68 61 49 47 42
37 29 21 21 20 13 8 7
BspEI T'CCGG_A
Cuts at: 5937 5937
Size: 6274
BspHI T'CATG_A
Cuts at: 4163 5893 4163
Size: 1730 4544
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2224 2499 2622 3629 844
Size: 1380 275 123 1007 3489
Fragments arranged by size:
3489 1380 1007 275 123
BsrBI CCG'CTC
Cuts at: 1394 1823 4161 1394
Size: 429 2338 3507
Fragments arranged by size:
3507 2338 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4769 4943 65
Size: 1183 3521 174 1396
Fragments arranged by size:
3521 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4918 5105 1700
Size: 110 3108 187 2869
Fragments arranged by size:
3108 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6274
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6274
BssSI C'ACGA_G
Cuts at: 4334 5346 4334
Size: 1012 5262
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3444 794
Size: 2650 3624
BstBI TT'CG_AA
Cuts at: 2358 5078 2358
Size: 2720 3554
BstEII G'GTnAC_C
Cuts at: 1184 2821 2953 1184
Size: 1637 132 4505
Fragments arranged by size:
4505 1637 132
BstXI CCAn_nnnn'nTGG
Cuts at: 2296 2296
Size: 6274
BstYI r'GATC_y
Cuts at: 1972 3604 3971 4356 4373 5825 5911 5923 6269
Size: 1632 367 385 17 1452 86 12 346
Cuts at: 6269 1972
Size: 1977
Fragments arranged by size:
1977 1632 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 3480 3480
Size: 6274
BtgI C'CryG_G
Cuts at: 513 1065 2004 2857 2964 513
Size: 552 939 853 107 3823
Fragments arranged by size:
3823 939 853 552 107
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2914 487
Size: 683 56 1688 3847
Fragments arranged by size:
3847 1688 683 56
BtsI GCAGTG_nn'
Cuts at: 3187 3243 3790 4584 4604 3187
Size: 56 547 794 20 4857
Fragments arranged by size:
4857 794 547 56 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6274
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6239
DraI TTT'AAA
Cuts at: 3578 3578
Size: 6274
DraIII CAC_nnn'GTG
Cuts at: 2740 2740
Size: 6274
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5281 809
Size: 507 3965 1802
Fragments arranged by size:
3965 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2662 2682 3721
Size: 54 1425 50 275 18 832 20 1039
Cuts at: 3721 4610 6239 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1039 889 832 275 54 50
43 20 18
EagI C'GGCC_G
Cuts at: 1537 1812 3721 1537
Size: 275 1909 4090
Fragments arranged by size:
4090 1909 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2076 2699 3217 4204 1353
Size: 723 623 518 987 3423
Fragments arranged by size:
3423 987 723 623 518
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4861 5235 5381 387
Size: 1064 138 3272 374 146 1280
Fragments arranged by size:
3272 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2692 3389 2692
Size: 697 5577
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2213 2829 3389 3396 3512 4337 4934
Size: 393 581 616 560 7 116 825 597
Cuts at: 4934 5721 5989 1239
Size: 787 268 1524
Fragments arranged by size:
1524 825 787 616 597 581 560 393
268 116 7
EcoNI CCTnn'n_nnAGG
Cuts at: 2231 3479 2231
Size: 1248 5026
EcoO109I rG'GnC_Cy
Cuts at: 2488 2721 3423 3562 2488
Size: 233 702 139 5200
Fragments arranged by size:
5200 702 233 139
EcoRI G'AATT_C
Cuts at: 3583 3961 3583
Size: 378 5896
FspI TGC'GCA
Cuts at: 2872 2981 4780 2872
Size: 109 1799 4366
Fragments arranged by size:
4366 1799 109
FspAI rTGC'GCAy
Cuts at: 2981 2981
Size: 6274
HaeII r_GCGC'y
Cuts at: 1678 2671 3119 5421 1678
Size: 993 448 2302 2531
Fragments arranged by size:
2531 2302 993 448
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2053 2085 4915 4947 4989
Size: 32 135 32 481 32 2830 32 42
Cuts at: 4989 5021 1373
Size: 32 2626
Fragments arranged by size:
2830 2626 481 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3618 3864 669
Size: 988 281 1680 246 3079
Fragments arranged by size:
3079 1680 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6274
HpaI GTT'AAC
Cuts at: 3864 3864
Size: 6274
KpnI G_GTAC'C
Cuts at: 3062 3599 3062
Size: 537 5737
MfeI C'AATT_G
Cuts at: 3873 3873
Size: 6274
MluI A'CGCG_T
Cuts at: 6150 6150
Size: 6274
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2096 5388 5572 1305
Size: 791 3292 184 2007
Fragments arranged by size:
3292 2007 791 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2664 2684 10
Size: 54 1425 343 832 20 3600
Fragments arranged by size:
3600 1425 832 343 54 20
MslI CAynn'nnrTG
Cuts at: 518 1166 2294 3203 4232 4591 4750 518
Size: 648 1128 909 1029 359 159 2042
Fragments arranged by size:
2042 1128 1029 909 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3558 3690 4367 5515 5760 1606
Size: 1952 132 677 1148 245 2120
Fragments arranged by size:
2120 1952 1148 677 245 132
NcoI C'CATG_G
Cuts at: 513 1065 2004 2857 2964 513
Size: 552 939 853 107 3823
Fragments arranged by size:
3823 939 853 552 107
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6274
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6274
NotI GC'GGCC_GC
Cuts at: 3721 3721
Size: 6274
NspI r_CATG'y
Cuts at: 1132 1229 2557 3447 3632 5177 1132
Size: 97 1328 890 185 1545 2229
Fragments arranged by size:
2229 1545 1328 890 185 97
PasI CC'CwG_GG
Cuts at: 1348 3054 3521 1348
Size: 1706 467 4101
Fragments arranged by size:
4101 1706 467
PciI A'CATG_T
Cuts at: 5173 5173
Size: 6274
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2172 2236 2637 2660 2671 1108
Size: 1064 64 401 23 11 4711
Fragments arranged by size:
4711 1064 401 64 23 11
PmeI GTTT'AAAC
Cuts at: 3578 3578
Size: 6274
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4343 4375 5856 5888 4343
Size: 32 1481 32 4729
Fragments arranged by size:
4729 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2488 2488
Size: 6274
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6274
PsiI TTA'TAA
Cuts at: 3844 3844
Size: 6274
PspOMI G'GGCC_C
Cuts at: 3147 3457 3561 3147
Size: 310 104 5860
Fragments arranged by size:
5860 310 104
PstI C_TGCA'G
Cuts at: 830 3235 3626 830
Size: 2405 391 3478
Fragments arranged by size:
3478 2405 391
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6274
PvuII CAG'CTG
Cuts at: 3558 3558
Size: 6274
SacI G_AGCT'C
Cuts at: 2694 3391 2694
Size: 697 5577
SalI G'TCGA_C
Cuts at: 1936 3616 1936
Size: 1680 4594
SapI GCTCTTCn'nnn_
Cuts at: 2699 3217 2699
Size: 518 5756
SbfI CC_TGCA'GG
Cuts at: 3626 3626
Size: 6274
ScaI AGT'ACT
Cuts at: 4522 6234 4522
Size: 1712 4562
SfcI C'TryA_G
Cuts at: 826 1045 2757 3231 3622 4757 5438 5629 826
Size: 219 1712 474 391 1135 681 191 1471
Fragments arranged by size:
1712 1471 1135 681 474 391 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6274
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6274
SmaI CCC'GGG
Cuts at: 1872 2711 3260 3601 1872
Size: 839 549 341 4545
Fragments arranged by size:
4545 839 549 341
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2765 4378 5279 5541 820
Size: 197 926 822 1613 901 262 1553
Fragments arranged by size:
1613 1553 926 901 822 262 197
SnaBI TAC'GTA
Cuts at: 493 4065 493
Size: 3572 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6274
SphI G_CATG'C
Cuts at: 1132 3632 1132
Size: 2500 3774
SspI AAT'ATT
Cuts at: 5 52 4198 5
Size: 47 4146 2081
Fragments arranged by size:
4146 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6274
StyI C'CwwG_G
Cuts at: 513 1065 2004 2125 2620 2857 2964 3161 3713
Size: 552 939 121 495 237 107 197 552
Cuts at: 3713 513
Size: 3074
Fragments arranged by size:
3074 939 552 552 495 237 197 121
107
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2179 3098 3540 4301 4318 4471 4656
Size: 1255 599 919 442 761 17 153 185
Cuts at: 4656 5101 325
Size: 445 1498
Fragments arranged by size:
1498 1255 919 761 599 445 442 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2544 4520 6232
Size: 274 80 33 51 1443 567 1976 1712
Cuts at: 6232 96
Size: 138
Fragments arranged by size:
1976 1712 1443 567 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3301 3318 3333 3350 6234 6266 3301
Size: 17 15 17 2884 32 3309
Fragments arranged by size:
3309 2884 32 17 17 15
Tth111I GACn'n_nGTC
Cuts at: 1316 3357 1316
Size: 2041 4233
XbaI T'CTAG_A
Cuts at: 3610 3610
Size: 6274
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2169 2936 3344 2169
Size: 767 408 5099
Fragments arranged by size:
5099 767 408
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6274
XmaI C'CCGG_G
Cuts at: 1870 2709 3258 3599 1870
Size: 839 549 341 4545
Fragments arranged by size:
4545 839 549 341
XmnI GAAnn'nnTTC
Cuts at: 4403 4403
Size: 6274
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4082 276
Size: 53 83 186 936 2548 2468
Fragments arranged by size:
2548 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI
BsiWI BsmBI BstZ17I EcoRV FalI FseI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI
PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI
SwaI