Restriction map for
pFN21AB9603
(Circular) (Six-base) MAPSORT of: pFN21AB9603.seq Check: 8239 from: 1 to: 5740
pFN21AB9603 5740 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3550 278
Size: 53 83 186 936 2014 2468
Fragments arranged by size:
2468 2014 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3083 1937
Size: 1146 4594
Acc65I G'GTAC_C
Cuts at: 3061 3061
Size: 5740
AclI AA'CG_TT
Cuts at: 1563 3867 4240 1563
Size: 2304 373 3063
Fragments arranged by size:
3063 2304 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2770 2817 3803 5187 5455 1632
Size: 1138 47 986 1384 268 1917
Fragments arranged by size:
1917 1384 1138 986 268 47
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5543
AflIII A'CryG_T
Cuts at: 1203 2397 4639 5616 1203
Size: 1194 2242 977 1327
Fragments arranged by size:
2242 1327 1194 977
AgeI A'CCGG_T
Cuts at: 4571 4571
Size: 5740
AhdI GACnn_n'nnGTC
Cuts at: 4469 4469
Size: 5740
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5740
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5740
AlwNI CAG_nnn'CTG
Cuts at: 2370 3483 5055 2370
Size: 1113 1572 3055
Fragments arranged by size:
3055 1572 1113
ApaI G_GGCC'C
Cuts at: 2450 2458 2546 2582 2871 2450
Size: 8 88 36 289 5319
Fragments arranged by size:
5319 289 88 36 8
ApaLI G'TGCA_C
Cuts at: 3797 4953 3797
Size: 1156 4584
ApoI r'AATT_y
Cuts at: 1493 3049 3275 3427 5536 1493
Size: 1556 226 152 2109 1697
Fragments arranged by size:
2109 1697 1556 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4294 160
Size: 872 3262 1606
Fragments arranged by size:
3262 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5740
AvaI C'yCGr_G
Cuts at: 1870 1943 2072 2125 2201 2450 2539 2722 2978
Size: 73 129 53 76 249 89 183 256
Cuts at: 2978 3065 1870
Size: 87 4545
Fragments arranged by size:
4545 256 249 183 129 89 87 76
73 53
AvrII C'CTAG_G
Cuts at: 2848 2848
Size: 5740
BamHI G'GATC_C
Cuts at: 2982 3070 3437 2982
Size: 88 367 5285
Fragments arranged by size:
5285 367 88
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2332 2421 2614 2855 2885
Size: 325 217 627 545 89 193 241 30
Cuts at: 2885 3061 4516 618
Size: 176 1455 1842
Fragments arranged by size:
1842 1455 627 545 325 241 217 193
176 89 30
BanII G_rGCy'C
Cuts at: 1391 2450 2458 2540 2546 2582 2768 2871 3059
Size: 1059 8 82 6 36 186 103 188
Cuts at: 3059 1391
Size: 4072
Fragments arranged by size:
4072 1059 188 186 103 82 36 8
6
BbeI G_GCGC'C
Cuts at: 2425 2618 2425
Size: 193 5547
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4769
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2520 2554 3931 3965 1903
Size: 34 583 34 1377 34 3678
Fragments arranged by size:
3678 1377 583 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3632 4842 3632
Size: 1210 4530
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5740
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2108 2462 4351
Size: 107 122 71 631 605 436 354 1889
Cuts at: 4351 136
Size: 1525
Fragments arranged by size:
1889 1525 631 605 436 354 122 107
71
BglII A'GATC_T
Cuts at: 5735 5735
Size: 5740
BlpI GC'TnA_GC
Cuts at: 3157 3157
Size: 5740
Bme1580I G_kGCm'C
Cuts at: 1790 2337 2450 2458 2546 2582 2871 3801 4957
Size: 547 113 8 88 36 289 930 1156
Cuts at: 4957 1790
Size: 2573
Fragments arranged by size:
2573 1156 930 547 289 113 88 36
8
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5740
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3471 4429 442
Size: 416 53 83 399 24 2054 958 1753
Fragments arranged by size:
2054 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5740
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2893 4400 1239
Size: 1654 1507 2579
Fragments arranged by size:
2579 1654 1507
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3865 4730 5028 3865
Size: 865 298 4577
Fragments arranged by size:
4577 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2071 2132 4403 882
Size: 617 572 61 2271 2219
Fragments arranged by size:
2271 2219 617 572 61
BsaAI yAC'GTr
Cuts at: 493 1204 3531 493
Size: 711 2327 2702
Fragments arranged by size:
2702 2327 711
BsaBI GATnn'nnATC
Cuts at: 5734 5734
Size: 5740
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2422 2615 3547 3929
Size: 53 83 186 936 889 193 932 382
Cuts at: 3929 4564 275
Size: 635 1451
Fragments arranged by size:
1451 936 932 889 635 382 193 186
83 53
BsaWI w'CCGG_w
Cuts at: 4173 4571 4845 4992 5403 4173
Size: 398 274 147 411 4510
Fragments arranged by size:
4510 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2070 2100 1178
Size: 30 165 30 667 30 4818
Fragments arranged by size:
4818 667 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2896 1186
Size: 1710 4030
BseYI C'CCAG_C
Cuts at: 4943 5439 4943
Size: 496 5244
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5167
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2354 2685 2694 2878 3190 3951
Size: 275 186 353 331 9 184 312 761
Cuts at: 3951 4979 1540
Size: 1028 2301
Fragments arranged by size:
2301 1028 761 353 331 312 275 186
184 9
BsiHKAI G_wGCw'C
Cuts at: 2768 3801 3886 4957 2768
Size: 1033 85 1071 3551
Fragments arranged by size:
3551 1071 1033 85
BsmI GAATG_Cn'
Cuts at: 3251 3344 3251
Size: 93 5647
BsmBI CGTCTCn'nnnn_
Cuts at: 2477 2477
Size: 5740
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2337 2450 2458 2540 2546 2582 2768
Size: 399 547 113 8 82 6 36 186
Cuts at: 2768 2871 3059 3801 3886 4957 1391
Size: 103 188 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 547 399 188 186 113
103 85 82 36 8 6
BspEI T'CCGG_A
Cuts at: 5403 5403
Size: 5740
BspHI T'CATG_A
Cuts at: 3629 5359 3629
Size: 1730 4010
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3001 3095 844
Size: 2157 94 3489
Fragments arranged by size:
3489 2157 94
BsrBI CCG'CTC
Cuts at: 1394 1823 2526 3627 1394
Size: 429 703 1101 3507
Fragments arranged by size:
3507 1101 703 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4235 4409 65
Size: 1183 2987 174 1396
Fragments arranged by size:
2987 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2271 2418 2611 4384 4571 1700
Size: 110 461 147 193 1773 187 2869
Fragments arranged by size:
2869 1773 461 193 187 147 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5740
BssHII G'CGCG_C
Cuts at: 1925 2705 1925
Size: 780 4960
BssSI C'ACGA_G
Cuts at: 3800 4812 3800
Size: 1012 4728
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5740
BstBI TT'CG_AA
Cuts at: 4544 4544
Size: 5740
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5740
BstYI r'GATC_y
Cuts at: 1972 2982 3070 3437 3822 3839 5291 5377 5389
Size: 1010 88 367 385 17 1452 86 12
Cuts at: 5389 5735 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1010 385 367 346 88 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2106 2181 2574 2685 513
Size: 552 1041 75 393 111 3568
Fragments arranged by size:
3568 1041 552 393 111 75
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2152 2161 487
Size: 683 56 926 9 4066
Fragments arranged by size:
4066 926 683 56 9
BtsI GCAGTG_nn'
Cuts at: 3256 4050 4070 3256
Size: 794 20 4926
Fragments arranged by size:
4926 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5740
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5705
DraI TTT'AAA
Cuts at: 3044 3044
Size: 5740
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2324 2954 4747 809
Size: 507 1008 630 1793 1802
Fragments arranged by size:
1802 1793 1008 630 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2040 2351 2627
Size: 54 1425 50 275 18 210 311 276
Cuts at: 2627 2682 2695 2875 2898 3187 4076 5705 8
Size: 55 13 180 23 289 889 1629 43
Fragments arranged by size:
1629 1425 889 311 289 276 275 210
180 55 54 50 43 23 18 13
EagI C'GGCC_G
Cuts at: 1537 1812 2351 2682 2875 3187 1537
Size: 275 539 331 193 312 4090
Fragments arranged by size:
4090 539 331 312 275 193
EarI CTCTTCn'nnn_
Cuts at: 1353 2021 2423 2756 3670 1353
Size: 668 402 333 914 3423
Fragments arranged by size:
3423 914 668 402 333
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2285 2598 2910 4327 4701 4847
Size: 1064 138 696 313 312 1417 374 146
Cuts at: 4847 387
Size: 1280
Fragments arranged by size:
1417 1280 1064 696 374 313 312 146
138
EcoICRI GAG'CTC
Cuts at: 2766 2766
Size: 5740
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2770 2817 2893 3803 4400 5187 5455
Size: 393 1138 47 76 910 597 787 268
Cuts at: 5455 1239
Size: 1524
Fragments arranged by size:
1524 1138 910 787 597 393 268 76
47
EcoO109I rG'GnC_Cy
Cuts at: 2447 2454 2455 2472 2543 2579 2727 2447
Size: 7 1 17 71 36 148 5460
Fragments arranged by size:
5460 148 71 36 17 7 1
EcoRI G'AATT_C
Cuts at: 3049 3427 3049
Size: 378 5362
FspI TGC'GCA
Cuts at: 4246 4246
Size: 5740
HaeII r_GCGC'y
Cuts at: 1678 2425 2573 2618 4887 1678
Size: 747 148 45 2269 2531
Fragments arranged by size:
2531 2269 747 148 45
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2070 2102 2588 2620 4381
Size: 32 135 32 498 32 486 32 1761
Cuts at: 4381 4413 4455 4487 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1761 498 486 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3084 3330 669
Size: 988 281 1146 246 3079
Fragments arranged by size:
3079 1146 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5740
HpaI GTT'AAC
Cuts at: 3330 3330
Size: 5740
KasI G'GCGC_C
Cuts at: 2421 2614 2421
Size: 193 5547
KpnI G_GTAC'C
Cuts at: 3065 3065
Size: 5740
MfeI C'AATT_G
Cuts at: 3339 3339
Size: 5740
MluI A'CGCG_T
Cuts at: 5616 5616
Size: 5740
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2793 4854 5038 1305
Size: 1488 2061 184 2007
Fragments arranged by size:
2061 2007 1488 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3918
Fragments arranged by size:
3918 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2180 3698 4057 4216 518
Size: 648 1014 1518 359 159 2042
Fragments arranged by size:
2042 1518 1014 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2108 2388 2576 2681 3156 3833 4981 5226
Size: 502 280 188 105 475 677 1148 245
Cuts at: 5226 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 502 475 280 245 188
105
NaeI GCC'GGC
Cuts at: 2613 2613
Size: 5740
NarI GG'CG_CC
Cuts at: 2422 2615 2422
Size: 193 5547
NcoI C'CATG_G
Cuts at: 513 1065 2181 513
Size: 552 1116 4072
Fragments arranged by size:
4072 1116 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5740
NgoMIV G'CCGG_C
Cuts at: 2611 2611
Size: 5740
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5740
NotI GC'GGCC_GC
Cuts at: 3187 3187
Size: 5740
NspI r_CATG'y
Cuts at: 1132 1229 2401 2957 3098 4643 1132
Size: 97 1172 556 141 1545 2229
Fragments arranged by size:
2229 1545 1172 556 141 97
PasI CC'CwG_GG
Cuts at: 1348 2671 1348
Size: 1323 4417
PciI A'CATG_T
Cuts at: 2397 4639 2397
Size: 2242 3498
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5740
PfoI T'CCnGG_A
Cuts at: 2190 2592 2190
Size: 402 5338
PmeI GTTT'AAAC
Cuts at: 3044 3044
Size: 5740
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3809 3841 5322 5354 3809
Size: 32 1481 32 4195
Fragments arranged by size:
4195 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2727 2727
Size: 5740
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5740
PsiI TTA'TAA
Cuts at: 3310 3310
Size: 5740
PspOMI G'GGCC_C
Cuts at: 2446 2454 2542 2578 2867 2446
Size: 8 88 36 289 5319
Fragments arranged by size:
5319 289 88 36 8
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2399 2431 2399
Size: 32 5708
PstI C_TGCA'G
Cuts at: 830 2565 3012 3092 830
Size: 1735 447 80 3478
Fragments arranged by size:
3478 1735 447 80
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5740
SacI G_AGCT'C
Cuts at: 2768 2768
Size: 5740
SacII CC_GC'GG
Cuts at: 2109 2577 2109
Size: 468 5272
SalI G'TCGA_C
Cuts at: 1936 3082 1936
Size: 1146 4594
SanDI GG'GwC_CC
Cuts at: 2727 2727
Size: 5740
SapI GCTCTTCn'nnn_
Cuts at: 2756 2756
Size: 5740
SbfI CC_TGCA'GG
Cuts at: 2565 3012 3092 2565
Size: 447 80 5213
Fragments arranged by size:
5213 447 80
ScaI AGT'ACT
Cuts at: 3988 5700 3988
Size: 1712 4028
SfcI C'TryA_G
Cuts at: 826 1045 2158 2561 3008 3088 4223 4904 5095
Size: 219 1113 403 447 80 1135 681 191
Cuts at: 5095 826
Size: 1471
Fragments arranged by size:
1471 1135 1113 681 447 403 219 191
80
SfiI GGCCn_nnn'nGGCC
Cuts at: 2462 2462
Size: 5740
SfoI GGC'GCC
Cuts at: 2423 2616 2423
Size: 193 5547
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5740
SgrAI Cr'CCGG_yG
Cuts at: 1700 2611 1700
Size: 911 4829
SmaI CCC'GGG
Cuts at: 1872 2452 2724 3067 1872
Size: 580 272 343 4545
Fragments arranged by size:
4545 580 343 272
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3844 4745 5007 820
Size: 197 926 1901 901 262 1553
Fragments arranged by size:
1901 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3531 493
Size: 3038 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5740
SphI G_CATG'C
Cuts at: 1132 3098 1132
Size: 1966 3774
SspI AAT'ATT
Cuts at: 5 52 3664 5
Size: 47 3612 2081
Fragments arranged by size:
3612 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5740
StyI C'CwwG_G
Cuts at: 513 1065 2181 2848 3179 513
Size: 552 1116 667 331 3074
Fragments arranged by size:
3074 1116 667 552 331
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2873 3767 3784 3937 4122 4567 325
Size: 1255 1293 894 17 153 185 445 1498
Fragments arranged by size:
1498 1293 1255 894 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3986 5698 96
Size: 274 80 33 51 1443 2009 1712 138
Fragments arranged by size:
2009 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5700 5732 5700
Size: 32 5708
Tth111I GACn'n_nGTC
Cuts at: 1316 2638 1316
Size: 1322 4418
XbaI T'CTAG_A
Cuts at: 3076 3076
Size: 5740
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5740
XmaI C'CCGG_G
Cuts at: 1870 2450 2722 3065 1870
Size: 580 272 343 4545
Fragments arranged by size:
4545 580 343 272
XmnI GAAnn'nnTTC
Cuts at: 3869 3869
Size: 5740
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3548 276
Size: 53 83 186 936 2014 2468
Fragments arranged by size:
2468 2014 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI
PvuI SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV
FalI FseI FspAI NruI NsiI PacI PmlI PspXI
PvuII RsrII SexAI SrfI SwaI XcmI