Restriction map for
pFN21AB9602
(Circular) (Six-base) MAPSORT of: pFN21AB9602.seq Check: 9416 from: 1 to: 6172
pFN21AB9602 6172 bp
With 182 enzymes: SgfI *
December 8, 2014 13:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3982 278
Size: 53 83 186 936 2446 2468
Fragments arranged by size:
2468 2446 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3515 1937
Size: 1578 4594
Acc65I G'GTAC_C
Cuts at: 3493 3493
Size: 6172
AclI AA'CG_TT
Cuts at: 1563 4299 4672 1563
Size: 2736 373 3063
Fragments arranged by size:
3063 2736 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4235 5619 5887 1632
Size: 2603 1384 268 1917
Fragments arranged by size:
2603 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5975
AflIII A'CryG_T
Cuts at: 1203 2793 3469 5071 6048 1203
Size: 1590 676 1602 977 1327
Fragments arranged by size:
1602 1590 1327 977 676
AgeI A'CCGG_T
Cuts at: 5003 5003
Size: 6172
AhdI GACnn_n'nnGTC
Cuts at: 4901 4901
Size: 6172
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6172
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6172
AlwNI CAG_nnn'CTG
Cuts at: 2308 2311 3915 5487 2308
Size: 3 1604 1572 2993
Fragments arranged by size:
2993 1604 1572 3
ApaI G_GGCC'C
Cuts at: 2300 2300
Size: 6172
ApaLI G'TGCA_C
Cuts at: 4229 5385 4229
Size: 1156 5016
ApoI r'AATT_y
Cuts at: 1493 3481 3707 3859 5968 1493
Size: 1988 226 152 2109 1697
Fragments arranged by size:
2109 1988 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4726 160
Size: 872 3694 1606
Fragments arranged by size:
3694 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6172
AvaI C'yCGr_G
Cuts at: 1870 1943 2078 2597 2749 3497 1870
Size: 73 135 519 152 748 4545
Fragments arranged by size:
4545 748 519 152 135 73
BamHI G'GATC_C
Cuts at: 3502 3869 3502
Size: 367 5805
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2074 2245 2270 2416 2447
Size: 325 217 627 287 171 25 146 31
Cuts at: 2447 2453 2728 2807 2826 2990 3166 3184 3493
Size: 6 275 79 19 164 176 18 309
Cuts at: 3493 4948 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 325 309 287 275 217
176 171 164 146 79 31 25 19
18 6
BanII G_rGCy'C
Cuts at: 1391 2300 2597 3491 1391
Size: 909 297 894 4072
Fragments arranged by size:
4072 909 894 297
BbeI G_GCGC'C
Cuts at: 2274 2420 2451 2457 2830 2274
Size: 146 31 6 373 5616
Fragments arranged by size:
5616 373 146 31 6
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3441 928
Size: 971 1542 3659
Fragments arranged by size:
3659 1542 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4363 4397 1903
Size: 34 50 34 2342 34 3678
Fragments arranged by size:
3678 2342 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4064 5274 4064
Size: 1210 4962
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6172
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2425 2855 4783
Size: 107 122 71 631 605 753 430 1928
Cuts at: 4783 136
Size: 1525
Fragments arranged by size:
1928 1525 753 631 605 430 122 107
71
BglII A'GATC_T
Cuts at: 6167 6167
Size: 6172
BlpI GC'TnA_GC
Cuts at: 3589 3589
Size: 6172
Bme1580I G_kGCm'C
Cuts at: 1790 2079 2300 3171 3189 4233 5389 1790
Size: 289 221 871 18 1044 1156 2573
Fragments arranged by size:
2573 1156 1044 871 289 221 18
BmgBI CAC'GTC
Cuts at: 1318 2794 2997 3265 3300 3333 1318
Size: 1476 203 268 35 33 4157
Fragments arranged by size:
4157 1476 268 203 35 33
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3903 4861 442
Size: 416 53 83 399 24 2486 958 1753
Fragments arranged by size:
2486 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3158 1056
Size: 2102 4070
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4832 1239
Size: 3593 2579
Bpu10I CC'TnA_GC
Cuts at: 3089 3089
Size: 6172
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4297 5162 5460 4297
Size: 865 298 5009
Fragments arranged by size:
5009 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4835 882
Size: 617 3336 2219
Fragments arranged by size:
3336 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3963 493
Size: 711 2759 2702
Fragments arranged by size:
2759 2702 711
BsaBI GATnn'nnATC
Cuts at: 2582 6166 2582
Size: 3584 2588
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2271 2417 2448 2454
Size: 53 83 186 936 738 146 31 6
Cuts at: 2454 2827 3979 4361 4996 275
Size: 373 1152 382 635 1451
Fragments arranged by size:
1451 1152 936 738 635 382 373 186
146 83 53 31 6
BsaWI w'CCGG_w
Cuts at: 4605 5003 5277 5424 5835 4605
Size: 398 274 147 411 4942
Fragments arranged by size:
4942 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3050 3056 3080 3086 3107
Size: 30 165 30 1647 6 24 6 21
Cuts at: 3107 3137 3203 3233 1178
Size: 30 66 30 4117
Fragments arranged by size:
4117 1647 165 66 30 30 30 30
24 21 6 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2480 3316 1186
Size: 1294 836 4042
Fragments arranged by size:
4042 1294 836
BseYI C'CCAG_C
Cuts at: 2139 2301 2374 3008 3052 5375 5871 2139
Size: 162 73 634 44 2323 496 2440
Fragments arranged by size:
2440 2323 634 496 162 73 44
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2936 1163
Size: 573 1200 4399
Fragments arranged by size:
4399 1200 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2280 2343 3147 3622 4383 5411
Size: 275 186 279 63 804 475 761 1028
Cuts at: 5411 1540
Size: 2301
Fragments arranged by size:
2301 1028 804 761 475 279 275 186
63
BsiHKAI G_wGCw'C
Cuts at: 4233 4318 5389 4233
Size: 85 1071 5016
Fragments arranged by size:
5016 1071 85
BsiWI C'GTAC_G
Cuts at: 3123 3123
Size: 6172
BsmI GAATG_Cn'
Cuts at: 3683 3776 3683
Size: 93 6079
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2079 2300 2597 3171 3189 3491 4233
Size: 399 289 221 297 574 18 302 742
Cuts at: 4233 4318 5389 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 574 399 302 297 289
221 85 18
BspEI T'CCGG_A
Cuts at: 5835 5835
Size: 6172
BspHI T'CATG_A
Cuts at: 2574 2637 4061 5791 2574
Size: 63 1424 1730 2955
Fragments arranged by size:
2955 1730 1424 63
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3237 3527 844
Size: 2393 290 3489
Fragments arranged by size:
3489 2393 290
BsrBI CCG'CTC
Cuts at: 1394 1823 2832 4059 1394
Size: 429 1009 1227 3507
Fragments arranged by size:
3507 1227 1009 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4667 4841 65
Size: 1183 3419 174 1396
Fragments arranged by size:
3419 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2338 2450 2888 2912 3099 4816 5003
Size: 110 528 112 438 24 187 1717 187
Cuts at: 5003 1700
Size: 2869
Fragments arranged by size:
2869 1717 528 438 187 187 112 110
24
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6172
BssHII G'CGCG_C
Cuts at: 1925 2873 1925
Size: 948 5224
BssSI C'ACGA_G
Cuts at: 4232 5244 4232
Size: 1012 5160
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2311 794
Size: 1517 4655
BstBI TT'CG_AA
Cuts at: 4976 4976
Size: 6172
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6172
BstYI r'GATC_y
Cuts at: 1972 3117 3502 3869 4254 4271 5723 5809 5821
Size: 1145 385 367 385 17 1452 86 12
Cuts at: 5821 6167 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1145 385 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2113 2811 513
Size: 552 1048 698 3874
Fragments arranged by size:
3874 1048 698 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5433
Fragments arranged by size:
5433 683 56
BtsI GCAGTG_nn'
Cuts at: 3688 4482 4502 3688
Size: 794 20 5358
Fragments arranged by size:
5358 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6172
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6137
DraI TTT'AAA
Cuts at: 3476 3476
Size: 6172
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3104 3403 5179 809
Size: 507 1788 299 1776 1802
Fragments arranged by size:
1802 1788 1776 507 299
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2116 2277 2340
Size: 54 1425 50 275 18 286 161 63
Cuts at: 2340 2580 3144 3619 4508 6137 8
Size: 240 564 475 889 1629 43
Fragments arranged by size:
1629 1425 889 564 475 286 275 240
161 63 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2277 2340 3144 3619 1537
Size: 275 465 63 804 475 4090
Fragments arranged by size:
4090 804 475 465 275 63
EarI CTCTTCn'nnn_
Cuts at: 1353 4102 1353
Size: 2749 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2681 4759 5133 5279 387
Size: 1064 138 1092 2078 374 146 1280
Fragments arranged by size:
2078 1280 1092 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 4235 4832 5619 5887 1239
Size: 393 2603 597 787 268 1524
Fragments arranged by size:
2603 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2296 2297 3198 3343 2296
Size: 1 901 145 5125
Fragments arranged by size:
5125 901 145 1
EcoRI G'AATT_C
Cuts at: 3481 3859 3481
Size: 378 5794
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2875 3396 2875
Size: 521 5651
FspI TGC'GCA
Cuts at: 4678 4678
Size: 6172
HaeII r_GCGC'y
Cuts at: 1678 2274 2420 2451 2457 2830 3312 5319 1678
Size: 596 146 31 6 373 482 2007 2531
Fragments arranged by size:
2531 2007 596 482 373 146 31 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4813 4845 4887 4919 1373
Size: 32 135 32 3241 32 42 32 2626
Fragments arranged by size:
3241 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3039 3109 3226 3516 3762 669
Size: 988 281 1101 70 117 290 246 3079
Fragments arranged by size:
3079 1101 988 290 281 246 117 70
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6172
HpaI GTT'AAC
Cuts at: 3762 3762
Size: 6172
KasI G'GCGC_C
Cuts at: 2270 2416 2447 2453 2826 2270
Size: 146 31 6 373 5616
Fragments arranged by size:
5616 373 146 31 6
KpnI G_GTAC'C
Cuts at: 3497 3497
Size: 6172
MfeI C'AATT_G
Cuts at: 3771 3771
Size: 6172
MluI A'CGCG_T
Cuts at: 6048 6048
Size: 6172
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3480 5286 5470 1305
Size: 2175 1806 184 2007
Fragments arranged by size:
2175 2007 1806 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2582 10
Size: 54 1425 343 750 3600
Fragments arranged by size:
3600 1425 750 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2576 2900 3302 4130 4489 4648 518
Size: 648 1410 324 402 828 359 159 2042
Fragments arranged by size:
2042 1410 828 648 402 359 324 159
MspA1I CmG'CkG
Cuts at: 1606 2308 3588 4265 5413 5658 1606
Size: 702 1280 677 1148 245 2120
Fragments arranged by size:
2120 1280 1148 702 677 245
NaeI GCC'GGC
Cuts at: 2452 2914 2452
Size: 462 5710
NarI GG'CG_CC
Cuts at: 2271 2417 2448 2454 2827 2271
Size: 146 31 6 373 5616
Fragments arranged by size:
5616 373 146 31 6
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5620
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6172
NgoMIV G'CCGG_C
Cuts at: 2450 2912 2450
Size: 462 5710
NheI G'CTAG_C
Cuts at: 1052 3154 1052
Size: 2102 4070
NotI GC'GGCC_GC
Cuts at: 3619 3619
Size: 6172
NspI r_CATG'y
Cuts at: 1132 1229 3473 3530 5075 1132
Size: 97 2244 57 1545 2229
Fragments arranged by size:
2244 2229 1545 97 57
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6172
PciI A'CATG_T
Cuts at: 3469 5071 3469
Size: 1602 4570
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6172
PmeI GTTT'AAAC
Cuts at: 3476 3476
Size: 6172
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4241 4273 5754 5786 4241
Size: 32 1481 32 4627
Fragments arranged by size:
4627 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3198 3198
Size: 6172
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6172
PsiI TTA'TAA
Cuts at: 3742 3742
Size: 6172
PspOMI G'GGCC_C
Cuts at: 2296 2296
Size: 6172
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2522 2554 2636 2668 2522
Size: 32 82 32 6026
Fragments arranged by size:
6026 82 32 32
PstI C_TGCA'G
Cuts at: 830 3524 830
Size: 2694 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6172
PvuII CAG'CTG
Cuts at: 2308 2308
Size: 6172
SalI G'TCGA_C
Cuts at: 1936 3514 1936
Size: 1578 4594
SbfI CC_TGCA'GG
Cuts at: 3524 3524
Size: 6172
ScaI AGT'ACT
Cuts at: 4420 6132 4420
Size: 1712 4460
SfcI C'TryA_G
Cuts at: 826 1045 3025 3520 4655 5336 5527 826
Size: 219 1980 495 1135 681 191 1471
Fragments arranged by size:
1980 1471 1135 681 495 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2855 2855
Size: 6172
SfoI GGC'GCC
Cuts at: 2272 2418 2449 2455 2828 2272
Size: 146 31 6 373 5616
Fragments arranged by size:
5616 373 146 31 6
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6172
SgrAI Cr'CCGG_yG
Cuts at: 1700 2450 1700
Size: 750 5422
SmaI CCC'GGG
Cuts at: 1872 2751 3499 1872
Size: 879 748 4545
Fragments arranged by size:
4545 879 748
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4276 5177 5439 820
Size: 197 926 2333 901 262 1553
Fragments arranged by size:
2333 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3963 493
Size: 3470 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6172
SphI G_CATG'C
Cuts at: 1132 3530 1132
Size: 2398 3774
SspI AAT'ATT
Cuts at: 5 52 4096 5
Size: 47 4044 2081
Fragments arranged by size:
4044 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6172
StyI C'CwwG_G
Cuts at: 513 1065 3611 513
Size: 552 2546 3074
Fragments arranged by size:
3074 2546 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4199 4216 4369 4554 4999 325
Size: 1255 2619 17 153 185 445 1498
Fragments arranged by size:
2619 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4418 6130 96
Size: 274 80 33 51 1443 2441 1712 138
Fragments arranged by size:
2441 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2687 2719 3056 3088 6132 6164 2687
Size: 32 337 32 3044 32 2695
Fragments arranged by size:
3044 2695 337 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6172
XbaI T'CTAG_A
Cuts at: 3508 3508
Size: 6172
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 6172
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6172
XmaI C'CCGG_G
Cuts at: 1870 2749 3497 1870
Size: 879 748 4545
Fragments arranged by size:
4545 879 748
XmnI GAAnn'nnTTC
Cuts at: 2645 4301 2645
Size: 1656 4516
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3980 276
Size: 53 83 186 936 2446 2468
Fragments arranged by size:
2468 2446 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI
PvuII SalI SbfI ScaI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FseI FspAI NruI NsiI PacI PfoI PmlI
PspXI RsrII SacI SacII SanDI SapI SexAI SrfI
SwaI