Restriction map for
pFN21AB9600
(Circular) (Six-base) MAPSORT of: pFN21AB9600.seq Check: 8552 from: 1 to: 5824
pFN21AB9600 5824 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3634 278
Size: 53 83 186 936 2098 2468
Fragments arranged by size:
2468 2098 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2637 3167 1937
Size: 700 530 4594
Fragments arranged by size:
4594 700 530
Acc65I G'GTAC_C
Cuts at: 3145 3145
Size: 5824
AclI AA'CG_TT
Cuts at: 1563 3951 4324 1563
Size: 2388 373 3063
Fragments arranged by size:
3063 2388 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3887 5271 5539 1632
Size: 2255 1384 268 1917
Fragments arranged by size:
2255 1917 1384 268
AfeI AGC'GCT
Cuts at: 2261 2447 2456 3009 2261
Size: 186 9 553 5076
Fragments arranged by size:
5076 553 186 9
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5627
AflIII A'CryG_T
Cuts at: 1203 2409 4723 5700 1203
Size: 1206 2314 977 1327
Fragments arranged by size:
2314 1327 1206 977
AgeI A'CCGG_T
Cuts at: 4655 4655
Size: 5824
AhdI GACnn_n'nnGTC
Cuts at: 4553 4553
Size: 5824
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5824
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2447 1184
Size: 1263 4561
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2396 2428 2396
Size: 32 5792
AlwNI CAG_nnn'CTG
Cuts at: 3567 5139 3567
Size: 1572 4252
ApaI G_GGCC'C
Cuts at: 2718 2733 2812 2855 2718
Size: 15 79 43 5687
Fragments arranged by size:
5687 79 43 15
ApaLI G'TGCA_C
Cuts at: 3881 5037 3881
Size: 1156 4668
ApoI r'AATT_y
Cuts at: 1493 3133 3359 3511 5620 1493
Size: 1640 226 152 2109 1697
Fragments arranged by size:
2109 1697 1640 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4378 160
Size: 872 3346 1606
Fragments arranged by size:
3346 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5824
AvaI C'yCGr_G
Cuts at: 1870 1943 2213 2365 2581 3149 1870
Size: 73 270 152 216 568 4545
Fragments arranged by size:
4545 568 270 216 152 73
BamHI G'GATC_C
Cuts at: 3154 3521 3154
Size: 367 5457
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2133 2557 2585 2762 2876
Size: 325 217 627 346 424 28 177 114
Cuts at: 2876 3059 3145 4600 618
Size: 183 86 1455 1842
Fragments arranged by size:
1842 1455 627 424 346 325 217 183
177 114 86 28
BanII G_rGCy'C
Cuts at: 1391 2718 2733 2812 2855 3143 1391
Size: 1327 15 79 43 288 4072
Fragments arranged by size:
4072 1327 288 79 43 15
BbeI G_GCGC'C
Cuts at: 2137 2589 2766 2880 3063 2137
Size: 452 177 114 183 4898
Fragments arranged by size:
4898 452 183 177 114
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2665 928
Size: 971 766 4087
Fragments arranged by size:
4087 971 766
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4015 4049 1903
Size: 34 2078 34 3678
Fragments arranged by size:
3678 2078 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3716 4926 3716
Size: 1210 4614
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5824
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2816 2837 2859
Size: 107 122 71 631 605 1144 21 22
Cuts at: 2859 4435 136
Size: 1576 1525
Fragments arranged by size:
1576 1525 1144 631 605 122 107 71
22 21
BglII A'GATC_T
Cuts at: 5819 5819
Size: 5824
BlpI GC'TnA_GC
Cuts at: 2699 3241 2699
Size: 542 5282
Bme1580I G_kGCm'C
Cuts at: 1790 2562 2718 2733 2812 2855 3885 5041 1790
Size: 772 156 15 79 43 1030 1156 2573
Fragments arranged by size:
2573 1156 1030 772 156 79 43 15
BmgBI CAC'GTC
Cuts at: 1318 2988 1318
Size: 1670 4154
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2392 3555 4513
Size: 416 53 83 399 24 975 1163 958
Cuts at: 4513 442
Size: 1753
Fragments arranged by size:
1753 1163 975 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5824
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2616 4484 1239
Size: 1377 1868 2579
Fragments arranged by size:
2579 1868 1377
Bpu10I CC'TnA_GC
Cuts at: 2688 2688
Size: 5824
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2324 3949 4814 5112 2324
Size: 1625 865 298 3036
Fragments arranged by size:
3036 1625 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2954 4487 882
Size: 617 1455 1533 2219
Fragments arranged by size:
2219 1533 1455 617
BsaAI yAC'GTr
Cuts at: 493 1204 3615 493
Size: 711 2411 2702
Fragments arranged by size:
2702 2411 711
BsaBI GATnn'nnATC
Cuts at: 5818 5818
Size: 5824
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2134 2586 2651 2763
Size: 53 83 186 936 601 452 65 112
Cuts at: 2763 2877 3060 3631 4013 4648 275
Size: 114 183 571 382 635 1451
Fragments arranged by size:
1451 936 635 601 571 452 382 186
183 114 112 83 65 53
BsaWI w'CCGG_w
Cuts at: 4257 4655 4929 5076 5487 4257
Size: 398 274 147 411 4594
Fragments arranged by size:
4594 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2396 2426 1178
Size: 30 165 30 993 30 4576
Fragments arranged by size:
4576 993 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5824
BseYI C'CCAG_C
Cuts at: 2230 2710 2932 3010 5027 5523 2230
Size: 480 222 78 2017 496 2531
Fragments arranged by size:
2531 2017 496 480 222 78
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2785 3075 1163
Size: 573 1049 290 3912
Fragments arranged by size:
3912 1049 573 290
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2362 2696 2816 3274 4035 5063
Size: 275 186 361 334 120 458 761 1028
Cuts at: 5063 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 458 361 334 275 186
120
BsiHKAI G_wGCw'C
Cuts at: 3885 3970 5041 3885
Size: 85 1071 4668
Fragments arranged by size:
4668 1071 85
BsmI GAATG_Cn'
Cuts at: 3335 3428 3335
Size: 93 5731
BsmBI CGTCTCn'nnnn_
Cuts at: 2069 2069
Size: 5824
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2562 2718 2733 2812 2855 3143 3885
Size: 399 772 156 15 79 43 288 742
Cuts at: 3885 3970 5041 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 772 742 399 288 156 85
79 43 15
BspEI T'CCGG_A
Cuts at: 5487 5487
Size: 5824
BspHI T'CATG_A
Cuts at: 3713 5443 3713
Size: 1730 4094
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3179 844
Size: 2335 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2019 2421 2692 2698 3711 1394
Size: 429 196 402 271 6 1013 3507
Fragments arranged by size:
3507 1013 429 402 271 196 6
BsrDI GCAATG_nn'
Cuts at: 65 1248 4319 4493 65
Size: 1183 3071 174 1396
Fragments arranged by size:
3071 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2357 2759 2780 2815 2879 4468 4655
Size: 110 547 402 21 35 64 1589 187
Cuts at: 4655 1700
Size: 2869
Fragments arranged by size:
2869 1589 547 402 187 110 64 35
21
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5824
BssHII G'CGCG_C
Cuts at: 1925 2567 1925
Size: 642 5182
BssSI C'ACGA_G
Cuts at: 3884 4896 3884
Size: 1012 4812
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5824
BstBI TT'CG_AA
Cuts at: 4628 4628
Size: 5824
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5824
BstYI r'GATC_y
Cuts at: 1972 2343 3154 3521 3906 3923 5375 5461 5473
Size: 371 811 367 385 17 1452 86 12
Cuts at: 5473 5819 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 811 385 371 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2109 2116 2193 2646 2964 3120 513
Size: 552 1044 7 77 453 318 156 3217
Fragments arranged by size:
3217 1044 552 453 318 156 77 7
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2164 2173 2185 487
Size: 683 56 938 9 12 4126
Fragments arranged by size:
4126 938 683 56 12 9
BtsI GCAGTG_nn'
Cuts at: 2890 2953 3340 4134 4154 2890
Size: 63 387 794 20 4560
Fragments arranged by size:
4560 794 387 63 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5824
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5789
DraI TTT'AAA
Cuts at: 3128 3128
Size: 5824
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4831 809
Size: 507 3515 1802
Fragments arranged by size:
3515 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2196 2359 2619
Size: 54 1425 50 275 18 366 163 260
Cuts at: 2619 2693 2813 2817 2905 2995 3271 4160 5789
Size: 74 120 4 88 90 276 889 1629
Cuts at: 5789 8
Size: 43
Fragments arranged by size:
1629 1425 889 366 276 275 260 163
120 90 88 74 54 50 43 18
4
EagI C'GGCC_G
Cuts at: 1537 1812 2359 2693 2813 3271 1537
Size: 275 547 334 120 458 4090
Fragments arranged by size:
4090 547 458 334 275 120
EarI CTCTTCn'nnn_
Cuts at: 1353 2058 3754 1353
Size: 705 1696 3423
Fragments arranged by size:
3423 1696 705
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2297 2746 2818 3106 4411 4785
Size: 1064 138 708 449 72 288 1305 374
Cuts at: 4785 4931 387
Size: 146 1280
Fragments arranged by size:
1305 1280 1064 708 449 374 288 146
138 72
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2616 3887 4484 5271 5539 1239
Size: 393 984 1271 597 787 268 1524
Fragments arranged by size:
1524 1271 984 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2095 2714 2729 2808 2852 2918 2095
Size: 619 15 79 44 66 5001
Fragments arranged by size:
5001 619 79 66 44 15
EcoRI G'AATT_C
Cuts at: 3133 3511 3133
Size: 378 5446
FseI GG_CCGG'CC
Cuts at: 2361 2819 2361
Size: 458 5366
FspI TGC'GCA
Cuts at: 4330 4330
Size: 5824
HaeII r_GCGC'y
Cuts at: 1678 2137 2146 2221 2263 2390 2449 2458 2589
Size: 459 9 75 42 127 59 9 131
Cuts at: 2589 2766 2880 3011 3063 4971 1678
Size: 177 114 131 52 1908 2531
Fragments arranged by size:
2531 1908 459 177 131 131 127 114
75 59 52 42 9 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4465 4497 4539 4571 1373
Size: 32 135 32 2893 32 42 32 2626
Fragments arranged by size:
2893 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3168 3414 669
Size: 988 281 1230 246 3079
Fragments arranged by size:
3079 1230 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5824
HpaI GTT'AAC
Cuts at: 3414 3414
Size: 5824
KasI G'GCGC_C
Cuts at: 2133 2585 2762 2876 3059 2133
Size: 452 177 114 183 4898
Fragments arranged by size:
4898 452 183 177 114
KpnI G_GTAC'C
Cuts at: 3149 3149
Size: 5824
MfeI C'AATT_G
Cuts at: 3423 3423
Size: 5824
MluI A'CGCG_T
Cuts at: 5700 5700
Size: 5824
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4938 5122 1305
Size: 3633 184 2007
Fragments arranged by size:
3633 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2198 10
Size: 54 1425 343 366 3636
Fragments arranged by size:
3636 1425 366 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2192 3782 4141 4300 518
Size: 648 1026 1590 359 159 2042
Fragments arranged by size:
2042 1590 1026 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2118 2648 2900 2936 3240 3917 5065 5310
Size: 512 530 252 36 304 677 1148 245
Cuts at: 5310 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 530 512 304 252 245
36
NaeI GCC'GGC
Cuts at: 2359 2761 2817 2881 2359
Size: 402 56 64 5302
Fragments arranged by size:
5302 402 64 56
NarI GG'CG_CC
Cuts at: 2134 2586 2763 2877 3060 2134
Size: 452 177 114 183 4898
Fragments arranged by size:
4898 452 183 177 114
NcoI C'CATG_G
Cuts at: 513 1065 2193 3120 513
Size: 552 1128 927 3217
Fragments arranged by size:
3217 1128 927 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5824
NgoMIV G'CCGG_C
Cuts at: 2357 2759 2815 2879 2357
Size: 402 56 64 5302
Fragments arranged by size:
5302 402 64 56
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5824
NotI GC'GGCC_GC
Cuts at: 2693 3271 2693
Size: 578 5246
NspI r_CATG'y
Cuts at: 1132 1229 2413 2488 3182 4727 1132
Size: 97 1184 75 694 1545 2229
Fragments arranged by size:
2229 1545 1184 694 97 75
PasI CC'CwG_GG
Cuts at: 1348 2099 1348
Size: 751 5073
PciI A'CATG_T
Cuts at: 2409 4723 2409
Size: 2314 3510
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2292 1108
Size: 1184 4640
PmeI GTTT'AAAC
Cuts at: 3128 3128
Size: 5824
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2396 2428 3893 3925 5406 5438 2396
Size: 32 1465 32 1481 32 2782
Fragments arranged by size:
2782 1481 1465 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2095 2918 2095
Size: 823 5001
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5824
PsiI TTA'TAA
Cuts at: 3394 3394
Size: 5824
PspOMI G'GGCC_C
Cuts at: 2714 2729 2808 2851 2714
Size: 15 79 43 5687
Fragments arranged by size:
5687 79 43 15
PstI C_TGCA'G
Cuts at: 830 2154 3176 830
Size: 1324 1022 3478
Fragments arranged by size:
3478 1324 1022
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5824
SacII CC_GC'GG
Cuts at: 2119 2649 2119
Size: 530 5294
SalI G'TCGA_C
Cuts at: 1936 3166 1936
Size: 1230 4594
SanDI GG'GwC_CC
Cuts at: 2095 2918 2095
Size: 823 5001
SapI GCTCTTCn'nnn_
Cuts at: 2058 2058
Size: 5824
SbfI CC_TGCA'GG
Cuts at: 3176 3176
Size: 5824
ScaI AGT'ACT
Cuts at: 4072 5784 4072
Size: 1712 4112
SfcI C'TryA_G
Cuts at: 826 1045 2150 2170 3172 4307 4988 5179 826
Size: 219 1105 20 1002 1135 681 191 1471
Fragments arranged by size:
1471 1135 1105 1002 681 219 191 20
SfiI GGCCn_nnn'nGGCC
Cuts at: 2816 2816
Size: 5824
SfoI GGC'GCC
Cuts at: 2135 2587 2764 2878 3061 2135
Size: 452 177 114 183 4898
Fragments arranged by size:
4898 452 183 177 114
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5824
SgrAI Cr'CCGG_yG
Cuts at: 1700 2759 1700
Size: 1059 4765
SmaI CCC'GGG
Cuts at: 1872 2367 2583 3151 1872
Size: 495 216 568 4545
Fragments arranged by size:
4545 568 495 216
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2339 3928 4829 5091 820
Size: 197 926 396 1589 901 262 1553
Fragments arranged by size:
1589 1553 926 901 396 262 197
SnaBI TAC'GTA
Cuts at: 493 3615 493
Size: 3122 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5824
SphI G_CATG'C
Cuts at: 1132 3182 1132
Size: 2050 3774
SspI AAT'ATT
Cuts at: 5 52 3748 5
Size: 47 3696 2081
Fragments arranged by size:
3696 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5824
StyI C'CwwG_G
Cuts at: 513 1065 2193 3120 3263 513
Size: 552 1128 927 143 3074
Fragments arranged by size:
3074 1128 927 552 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3851 3868 4021 4206 4651 325
Size: 1255 2271 17 153 185 445 1498
Fragments arranged by size:
2271 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4070 5782 96
Size: 274 80 33 51 1443 2093 1712 138
Fragments arranged by size:
2093 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5784 5816 5784
Size: 32 5792
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5824
XbaI T'CTAG_A
Cuts at: 3160 3160
Size: 5824
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5824
XmaI C'CCGG_G
Cuts at: 1870 2365 2581 3149 1870
Size: 495 216 568 4545
Fragments arranged by size:
4545 568 495 216
XmnI GAAnn'nnTTC
Cuts at: 3953 3953
Size: 5824
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3632 276
Size: 53 83 186 936 2098 2468
Fragments arranged by size:
2468 2098 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FseI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI SacII SalI SanDI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI
BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI
FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI
PvuII RsrII SacI SexAI SrfI SwaI XcmI