Restriction map for
pFN21AB9599
(Circular) (Six-base) MAPSORT of: pFN21AB9599.seq Check: 3585 from: 1 to: 6022
pFN21AB9599 6022 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2161 2643 2161
Size: 482 5540
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3832 278
Size: 53 83 186 936 2296 2468
Fragments arranged by size:
2468 2296 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3365 1937
Size: 1428 4594
Acc65I G'GTAC_C
Cuts at: 3343 3343
Size: 6022
AclI AA'CG_TT
Cuts at: 1563 4149 4522 1563
Size: 2586 373 3063
Fragments arranged by size:
3063 2586 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2313 4085 5469 5737 1632
Size: 681 1772 1384 268 1917
Fragments arranged by size:
1917 1772 1384 681 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5825
AflIII A'CryG_T
Cuts at: 1203 2562 4921 5898 1203
Size: 1359 2359 977 1327
Fragments arranged by size:
2359 1359 1327 977
AgeI A'CCGG_T
Cuts at: 4853 4853
Size: 6022
AhdI GACnn_n'nnGTC
Cuts at: 4751 4751
Size: 6022
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6022
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6022
AlwNI CAG_nnn'CTG
Cuts at: 3013 3765 5337 3013
Size: 752 1572 3698
Fragments arranged by size:
3698 1572 752
ApaI G_GGCC'C
Cuts at: 2710 3318 2710
Size: 608 5414
ApaLI G'TGCA_C
Cuts at: 4079 5235 4079
Size: 1156 4866
ApoI r'AATT_y
Cuts at: 1493 3331 3557 3709 5818 1493
Size: 1838 226 152 2109 1697
Fragments arranged by size:
2109 1838 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4576 160
Size: 872 3544 1606
Fragments arranged by size:
3544 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6022
AvaI C'yCGr_G
Cuts at: 1870 1943 2202 2230 2623 2731 2756 3347 1870
Size: 73 259 28 393 108 25 591 4545
Fragments arranged by size:
4545 591 393 259 108 73 28 25
BamHI G'GATC_C
Cuts at: 3352 3719 3352
Size: 367 5655
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2252 2266 2833 2872 3165
Size: 325 217 627 465 14 567 39 293
Cuts at: 3165 3343 4798 618
Size: 178 1455 1842
Fragments arranged by size:
1842 1455 627 567 465 325 293 217
178 39 14
BanII G_rGCy'C
Cuts at: 1391 2632 2710 3218 3318 3341 1391
Size: 1241 78 508 100 23 4072
Fragments arranged by size:
4072 1241 508 100 78 23
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2079 2118 928
Size: 971 180 39 4832
Fragments arranged by size:
4832 971 180 39
BbvCI CC'TCA_GC
Cuts at: 2906 2906
Size: 6022
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2777 2811 4213 4247 1903
Size: 34 840 34 1402 34 3678
Fragments arranged by size:
3678 1402 840 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3914 5124 3914
Size: 1210 4812
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6022
BfrBI ATG'CAT
Cuts at: 2957 2957
Size: 6022
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2520 3182 4633
Size: 107 122 71 631 605 848 662 1451
Cuts at: 4633 136
Size: 1525
Fragments arranged by size:
1525 1451 848 662 631 605 122 107
71
BglII A'GATC_T
Cuts at: 6017 6017
Size: 6022
BlpI GC'TnA_GC
Cuts at: 2022 2384 3439 2022
Size: 362 1055 4605
Fragments arranged by size:
4605 1055 362
Bme1580I G_kGCm'C
Cuts at: 1790 2269 2710 2836 3168 3318 4083 5239 1790
Size: 479 441 126 332 150 765 1156 2573
Fragments arranged by size:
2573 1156 765 479 441 332 150 126
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6022
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3753 4711 442
Size: 416 53 83 399 24 2336 958 1753
Fragments arranged by size:
2336 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6022
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3277 3277
Size: 6022
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2141 2557 3043 4682 1239
Size: 902 416 486 1639 2579
Fragments arranged by size:
2579 1639 902 486 416
Bpu10I CC'TnA_GC
Cuts at: 2906 2906
Size: 6022
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4147 5012 5310 4147
Size: 865 298 4859
Fragments arranged by size:
4859 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4685 882
Size: 617 3186 2219
Fragments arranged by size:
3186 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3813 493
Size: 711 2609 2702
Fragments arranged by size:
2702 2609 711
BsaBI GATnn'nnATC
Cuts at: 6016 6016
Size: 6022
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2764 2935 3829 4211
Size: 53 83 186 936 1231 171 894 382
Cuts at: 4211 4846 275
Size: 635 1451
Fragments arranged by size:
1451 1231 936 894 635 382 186 171
83 53
BsaWI w'CCGG_w
Cuts at: 4455 4853 5127 5274 5685 4455
Size: 398 274 147 411 4792
Fragments arranged by size:
4792 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2537 2567 1178
Size: 30 165 30 1134 30 4633
Fragments arranged by size:
4633 1134 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2114 2132 2135 2314 2784 2932 3128 1186
Size: 928 18 3 179 470 148 196 4080
Fragments arranged by size:
4080 928 470 196 179 148 18 3
BseYI C'CCAG_C
Cuts at: 3157 5225 5721 3157
Size: 2068 496 3458
Fragments arranged by size:
3458 2068 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2646 2660 2765 1163
Size: 573 910 14 105 4420
Fragments arranged by size:
4420 910 573 105 14
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3035 3067 3177 3472 4233 5261
Size: 275 186 1034 32 110 295 761 1028
Cuts at: 5261 1540
Size: 2301
Fragments arranged by size:
2301 1034 1028 761 295 275 186 110
32
BsiHKAI G_wGCw'C
Cuts at: 2211 4083 4168 5239 2211
Size: 1872 85 1071 2994
Fragments arranged by size:
2994 1872 1071 85
BsmI GAATG_Cn'
Cuts at: 3533 3626 3533
Size: 93 5929
BsmBI CGTCTCn'nnnn_
Cuts at: 2755 2755
Size: 6022
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2211 2269 2632 2710 2836 3168 3218
Size: 399 421 58 363 78 126 332 50
Cuts at: 3218 3318 3341 4083 4168 5239 1391
Size: 100 23 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 421 399 363 332 126
100 85 78 58 50 23
BspEI T'CCGG_A
Cuts at: 5685 5685
Size: 6022
BspHI T'CATG_A
Cuts at: 3911 5641 3911
Size: 1730 4292
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2161 2643 2665 3377 844
Size: 1317 482 22 712 3489
Fragments arranged by size:
3489 1317 712 482 22
BsrBI CCG'CTC
Cuts at: 1394 1823 3001 3909 1394
Size: 429 1178 908 3507
Fragments arranged by size:
3507 1178 908 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4517 4691 65
Size: 1183 3269 174 1396
Fragments arranged by size:
3269 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2308 4666 4853 1700
Size: 110 498 2358 187 2869
Fragments arranged by size:
2869 2358 498 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6022
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6022
BssSI C'ACGA_G
Cuts at: 4082 5094 4082
Size: 1012 5010
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6022
BstBI TT'CG_AA
Cuts at: 4826 4826
Size: 6022
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6022
BstYI r'GATC_y
Cuts at: 1972 2496 3352 3719 4104 4121 5573 5659 5671
Size: 524 856 367 385 17 1452 86 12
Cuts at: 5671 6017 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 856 524 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2346 513
Size: 552 1281 4189
Fragments arranged by size:
4189 1281 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2317 3213 487
Size: 683 56 1091 896 3296
Fragments arranged by size:
3296 1091 896 683 56
BtsI GCAGTG_nn'
Cuts at: 3538 4332 4352 3538
Size: 794 20 5208
Fragments arranged by size:
5208 794 20
ClaI AT'CG_AT
Cuts at: 1554 2085 1554
Size: 531 5491
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5987
DraI TTT'AAA
Cuts at: 3326 3326
Size: 6022
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2489 5029 809
Size: 507 1173 2540 1802
Fragments arranged by size:
2540 1802 1173 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2349 2410 3174
Size: 54 1425 50 275 18 519 61 764
Cuts at: 3174 3234 3300 3469 4358 5987 8
Size: 60 66 169 889 1629 43
Fragments arranged by size:
1629 1425 889 764 519 275 169 66
61 60 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3174 3469 1537
Size: 275 1362 295 4090
Fragments arranged by size:
4090 1362 295 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3952 1353
Size: 2599 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2450 2966 3264 4609 4983 5129
Size: 1064 138 861 516 298 1345 374 146
Cuts at: 5129 387
Size: 1280
Fragments arranged by size:
1345 1280 1064 861 516 374 298 146
138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2141 2313 2557 3043 4085 4682 5469
Size: 393 509 172 244 486 1042 597 787
Cuts at: 5469 5737 1239
Size: 268 1524
Fragments arranged by size:
1524 1042 787 597 509 486 393 268
244 172
EcoNI CCTnn'n_nnAGG
Cuts at: 2096 2096
Size: 6022
EcoO109I rG'GnC_Cy
Cuts at: 2706 2707 2736 3258 3314 3315 2706
Size: 1 29 522 56 1 5413
Fragments arranged by size:
5413 522 56 29 1 1
EcoRI G'AATT_C
Cuts at: 3331 3709 3331
Size: 378 5644
FspI TGC'GCA
Cuts at: 3017 4528 3017
Size: 1511 4511
HaeII r_GCGC'y
Cuts at: 1678 2029 2377 2611 3188 5169 1678
Size: 351 348 234 577 1981 2531
Fragments arranged by size:
2531 1981 577 351 348 234
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2196 2228 2537 2569 4663
Size: 32 135 32 624 32 309 32 2094
Cuts at: 4663 4695 4737 4769 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2094 624 309 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3366 3612 669
Size: 988 281 1428 246 3079
Fragments arranged by size:
3079 1428 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6022
HpaI GTT'AAC
Cuts at: 3612 3612
Size: 6022
KpnI G_GTAC'C
Cuts at: 3347 3347
Size: 6022
MfeI C'AATT_G
Cuts at: 3621 3621
Size: 6022
MluI A'CGCG_T
Cuts at: 5898 5898
Size: 6022
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3059 5136 5320 1305
Size: 1754 2077 184 2007
Fragments arranged by size:
2077 2007 1754 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2351 10
Size: 54 1425 343 519 3681
Fragments arranged by size:
3681 1425 519 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2345 3980 4339 4498 518
Size: 648 1179 1635 359 159 2042
Fragments arranged by size:
2042 1635 1179 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2388 3103 3438 4115 5263 5508 1606
Size: 782 715 335 677 1148 245 2120
Fragments arranged by size:
2120 1148 782 715 677 335 245
NaeI GCC'GGC
Cuts at: 2310 2310
Size: 6022
NcoI C'CATG_G
Cuts at: 513 1065 2346 513
Size: 552 1281 4189
Fragments arranged by size:
4189 1281 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6022
NgoMIV G'CCGG_C
Cuts at: 2308 2308
Size: 6022
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6022
NotI GC'GGCC_GC
Cuts at: 3469 3469
Size: 6022
NsiI A_TGCA'T
Cuts at: 2959 2959
Size: 6022
NspI r_CATG'y
Cuts at: 1132 1229 2566 3380 4925 1132
Size: 97 1337 814 1545 2229
Fragments arranged by size:
2229 1545 1337 814 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6022
PciI A'CATG_T
Cuts at: 2562 4921 2562
Size: 2359 3663
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6022
PfoI T'CCnGG_A
Cuts at: 2055 2055
Size: 6022
PmeI GTTT'AAAC
Cuts at: 3326 3326
Size: 6022
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4091 4123 5604 5636 4091
Size: 32 1481 32 4477
Fragments arranged by size:
4477 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3258 3258
Size: 6022
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6022
PsiI TTA'TAA
Cuts at: 3592 3592
Size: 6022
PspOMI G'GGCC_C
Cuts at: 2706 3314 2706
Size: 608 5414
PstI C_TGCA'G
Cuts at: 830 2172 2891 3374 830
Size: 1342 719 483 3478
Fragments arranged by size:
3478 1342 719 483
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6022
SalI G'TCGA_C
Cuts at: 1936 3364 1936
Size: 1428 4594
SbfI CC_TGCA'GG
Cuts at: 3374 3374
Size: 6022
ScaI AGT'ACT
Cuts at: 4270 5982 4270
Size: 1712 4310
SfcI C'TryA_G
Cuts at: 826 1045 2168 2887 3370 4505 5186 5377 826
Size: 219 1123 719 483 1135 681 191 1471
Fragments arranged by size:
1471 1135 1123 719 681 483 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6022
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6022
SmaI CCC'GGG
Cuts at: 1872 2625 2733 2758 3349 1872
Size: 753 108 25 591 4545
Fragments arranged by size:
4545 753 591 108 25
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4126 5027 5289 820
Size: 197 926 2183 901 262 1553
Fragments arranged by size:
2183 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3813 493
Size: 3320 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6022
SphI G_CATG'C
Cuts at: 1132 3380 1132
Size: 2248 3774
SspI AAT'ATT
Cuts at: 5 52 3946 5
Size: 47 3894 2081
Fragments arranged by size:
3894 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6022
StyI C'CwwG_G
Cuts at: 513 1065 2273 2346 2911 3144 3461 513
Size: 552 1208 73 565 233 317 3074
Fragments arranged by size:
3074 1208 565 552 317 233 73
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4049 4066 4219 4404 4849 325
Size: 1255 2469 17 153 185 445 1498
Fragments arranged by size:
2469 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4268 5980 96
Size: 274 80 33 51 1443 2291 1712 138
Fragments arranged by size:
2291 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5982 6014 5982
Size: 32 5990
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6022
XbaI T'CTAG_A
Cuts at: 3358 3358
Size: 6022
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6022
XmaI C'CCGG_G
Cuts at: 1870 2623 2731 2756 3347 1870
Size: 753 108 25 591 4545
Fragments arranged by size:
4545 753 591 108 25
XmnI GAAnn'nnTTC
Cuts at: 2600 4151 2600
Size: 1551 4471
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3830 276
Size: 53 83 186 936 2296 2468
Fragments arranged by size:
2468 2296 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BsiWI BstXI
BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI
KasI NarI NruI PacI PmlI PspXI PsrI PvuII
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI