Restriction map for
pFN21AB9596
(Circular) (Six-base) MAPSORT of: pFN21AB9596.seq Check: 3484 from: 1 to: 6208
pFN21AB9596 6208 bp
With 182 enzymes: SgfI *
March 28, 2012 12:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4018 278
Size: 53 83 186 936 2482 2468
Fragments arranged by size:
2482 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3551 1937
Size: 1614 4594
Acc65I G'GTAC_C
Cuts at: 3529 3529
Size: 6208
AclI AA'CG_TT
Cuts at: 1563 4335 4708 1563
Size: 2772 373 3063
Fragments arranged by size:
3063 2772 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2797 4271 5655 5923 1632
Size: 1165 1474 1384 268 1917
Fragments arranged by size:
1917 1474 1384 1165 268
AfeI AGC'GCT
Cuts at: 3115 3115
Size: 6208
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6011
AflIII A'CryG_T
Cuts at: 1203 2466 2520 3045 3318 5107 6084 1203
Size: 1263 54 525 273 1789 977 1327
Fragments arranged by size:
1789 1327 1263 977 525 273 54
AgeI A'CCGG_T
Cuts at: 5039 5039
Size: 6208
AhdI GACnn_n'nnGTC
Cuts at: 4937 4937
Size: 6208
AleI CACnn'nnGTG
Cuts at: 1166 2684 1166
Size: 1518 4690
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2504 1184
Size: 1320 4888
AlwNI CAG_nnn'CTG
Cuts at: 2439 2965 3951 5523 2439
Size: 526 986 1572 3124
Fragments arranged by size:
3124 1572 986 526
ApaI G_GGCC'C
Cuts at: 2548 2548
Size: 6208
ApaLI G'TGCA_C
Cuts at: 4265 5421 4265
Size: 1156 5052
ApoI r'AATT_y
Cuts at: 1493 3517 3743 3895 6004 1493
Size: 2024 226 152 2109 1697
Fragments arranged by size:
2109 2024 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4762 160
Size: 872 3730 1606
Fragments arranged by size:
3730 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6208
AvaI C'yCGr_G
Cuts at: 1870 1943 2270 2702 2869 3088 3245 3401 3533
Size: 73 327 432 167 219 157 156 132
Cuts at: 3533 1870
Size: 4545
Fragments arranged by size:
4545 432 327 219 167 157 156 132
73
BamHI G'GATC_C
Cuts at: 3538 3905 3538
Size: 367 5841
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2050 2200 2401 2493 2582
Size: 325 217 627 263 150 201 92 89
Cuts at: 2582 2791 2896 3199 3529 4984 618
Size: 209 105 303 330 1455 1842
Fragments arranged by size:
1842 1455 627 330 325 303 263 217
209 201 150 105 92 89
BanII G_rGCy'C
Cuts at: 1391 2548 2702 2870 3527 1391
Size: 1157 154 168 657 4072
Fragments arranged by size:
4072 1157 657 168 154
BbeI G_GCGC'C
Cuts at: 2204 2497 2586 2795 3203 2204
Size: 293 89 209 408 5209
Fragments arranged by size:
5209 408 293 209 89
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5237
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4399 4433 1903
Size: 34 50 34 2378 34 3678
Fragments arranged by size:
3678 2378 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4100 5310 4100
Size: 1210 4998
BclI T'GATC_A
Cuts at: 1548 2640 1548
Size: 1092 5116
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2118 2800
Size: 107 122 71 631 605 337 109 682
Cuts at: 2800 3141 3257 4819 136
Size: 341 116 1562 1525
Fragments arranged by size:
1562 1525 682 631 605 341 337 122
116 109 107 71
BglII A'GATC_T
Cuts at: 6203 6203
Size: 6208
BlpI GC'TnA_GC
Cuts at: 2696 2864 3625 2696
Size: 168 761 5279
Fragments arranged by size:
5279 761 168
Bme1580I G_kGCm'C
Cuts at: 1790 2055 2406 2548 4269 5425 1790
Size: 265 351 142 1721 1156 2573
Fragments arranged by size:
2573 1721 1156 351 265 142
BmgBI CAC'GTC
Cuts at: 1318 2521 3102 1318
Size: 1203 581 4424
Fragments arranged by size:
4424 1203 581
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3939 4897 442
Size: 416 53 83 399 24 2522 958 1753
Fragments arranged by size:
2522 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6208
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3094 3094
Size: 6208
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2987 4868 1239
Size: 1748 1881 2579
Fragments arranged by size:
2579 1881 1748
Bpu10I CC'TnA_GC
Cuts at: 2060 2958 3110 2060
Size: 898 152 5158
Fragments arranged by size:
5158 898 152
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2537 4333 5198 5496 2537
Size: 1796 865 298 3249
Fragments arranged by size:
3249 1796 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4871 882
Size: 617 3372 2219
Fragments arranged by size:
3372 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3999 493
Size: 711 2795 2702
Fragments arranged by size:
2795 2702 711
BsaBI GATnn'nnATC
Cuts at: 6202 6202
Size: 6208
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2121 2201 2494 2573
Size: 53 83 186 936 588 80 293 79
Cuts at: 2573 2583 2610 2657 2792 3200 3236 4015 4397
Size: 10 27 47 135 408 36 779 382
Cuts at: 4397 5032 275
Size: 635 1451
Fragments arranged by size:
1451 936 779 635 588 408 382 293
186 135 83 80 79 53 47 36
27 10
BsaWI w'CCGG_w
Cuts at: 4641 5039 5313 5460 5871 4641
Size: 398 274 147 411 4978
Fragments arranged by size:
4978 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2441 2471 1178
Size: 30 165 30 1038 30 4915
Fragments arranged by size:
4915 1038 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2552 1186
Size: 1366 4842
BseYI C'CCAG_C
Cuts at: 3222 3228 3330 3379 5411 5907 3222
Size: 6 102 49 2032 496 3523
Fragments arranged by size:
3523 2032 496 102 49 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3016 3481 1163
Size: 573 1280 465 3890
Fragments arranged by size:
3890 1280 573 465
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3038 3658 4419 5447 1540
Size: 275 186 1037 620 761 1028 2301
Fragments arranged by size:
2301 1037 1028 761 620 275 186
BsiHKAI G_wGCw'C
Cuts at: 2781 4269 4354 5425 2781
Size: 1488 85 1071 3564
Fragments arranged by size:
3564 1488 1071 85
BsmI GAATG_Cn'
Cuts at: 3719 3812 3719
Size: 93 6115
BsmBI CGTCTCn'nnnn_
Cuts at: 2684 2684
Size: 6208
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2055 2406 2548 2702 2781 2870 3527
Size: 399 265 351 142 154 79 89 657
Cuts at: 3527 4269 4354 5425 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 657 399 351 265 154
142 89 85 79
BspEI T'CCGG_A
Cuts at: 5871 5871
Size: 6208
BspHI T'CATG_A
Cuts at: 2829 4097 5827 2829
Size: 1268 1730 3210
Fragments arranged by size:
3210 1730 1268
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3563 844
Size: 2719 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2166 2542 3034 3093 4095 1394
Size: 429 343 376 492 59 1002 3507
Fragments arranged by size:
3507 1002 492 429 376 343 59
BsrDI GCAATG_nn'
Cuts at: 65 1248 3430 4703 4877 65
Size: 1183 2182 1273 174 1396
Fragments arranged by size:
2182 1396 1273 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2496 2671 2750 3037 3131 3214 3375
Size: 110 686 175 79 287 94 83 161
Cuts at: 3375 4852 5039 1700
Size: 1477 187 2869
Fragments arranged by size:
2869 1477 686 287 187 175 161 110
94 83 79
BsrGI T'GTAC_A
Cuts at: 96 3018 96
Size: 2922 3286
BssHII G'CGCG_C
Cuts at: 1925 2012 2709 3144 1925
Size: 87 697 435 4989
Fragments arranged by size:
4989 697 435 87
BssSI C'ACGA_G
Cuts at: 4268 5280 4268
Size: 1012 5196
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6208
BstBI TT'CG_AA
Cuts at: 5012 5012
Size: 6208
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6208
BstXI CCAn_nnnn'nTGG
Cuts at: 2041 2116 3419 2041
Size: 75 1303 4830
Fragments arranged by size:
4830 1303 75
BstYI r'GATC_y
Cuts at: 1972 3449 3538 3905 4290 4307 5759 5845 5857
Size: 1477 89 367 385 17 1452 86 12
Cuts at: 5857 6203 1972
Size: 346 1977
Fragments arranged by size:
1977 1477 1452 385 367 346 89 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2205 2205
Size: 6208
BtgI C'CryG_G
Cuts at: 513 1065 2115 2196 2250 2787 3156 3178 3255
Size: 552 1050 81 54 537 369 22 77
Cuts at: 3255 513
Size: 3466
Fragments arranged by size:
3466 1050 552 537 369 81 77 54
22
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2221 3023 487
Size: 683 56 995 802 3672
Fragments arranged by size:
3672 995 802 683 56
BtsI GCAGTG_nn'
Cuts at: 3724 4518 4538 3724
Size: 794 20 5394
Fragments arranged by size:
5394 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6208
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6173
DraI TTT'AAA
Cuts at: 3512 3512
Size: 6208
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5215 809
Size: 507 3899 1802
Fragments arranged by size:
3899 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2106 2131 2253
Size: 54 1425 50 275 18 276 25 122
Cuts at: 2253 2589 2944 3035 3133 3181 3313 3655 4544
Size: 336 355 91 98 48 132 342 889
Cuts at: 4544 6173 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 355 342 336 276 275
132 122 98 91 54 50 48 43
25 18
EagI C'GGCC_G
Cuts at: 1537 1812 3035 3655 1537
Size: 275 1223 620 4090
Fragments arranged by size:
4090 1223 620 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4138 1353
Size: 2785 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2354 2871 4795 5169 5315 387
Size: 1064 138 765 517 1924 374 146 1280
Fragments arranged by size:
1924 1280 1064 765 517 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2797 2987 4271 4868 5655 5923 1239
Size: 393 1165 190 1284 597 787 268 1524
Fragments arranged by size:
1524 1284 1165 787 597 393 268 190
EcoNI CCTnn'n_nnAGG
Cuts at: 3068 3068
Size: 6208
EcoO109I rG'GnC_Cy
Cuts at: 2209 2962 3073 3295 2209
Size: 753 111 222 5122
Fragments arranged by size:
5122 753 222 111
EcoRI G'AATT_C
Cuts at: 3517 3895 3517
Size: 378 5830
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2302 2302
Size: 6208
FspI TGC'GCA
Cuts at: 4714 4714
Size: 6208
HaeII r_GCGC'y
Cuts at: 1678 2204 2497 2506 2586 2669 2795 2912 3117
Size: 526 293 9 80 83 126 117 205
Cuts at: 3117 3203 5355 1678
Size: 86 2152 2531
Fragments arranged by size:
2531 2152 526 293 205 126 117 86
83 80 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2441 2473 2642 2674 4849
Size: 32 135 32 869 32 169 32 2175
Cuts at: 4849 4881 4923 4955 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2175 869 169 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3552 3798 669
Size: 988 281 1614 246 3079
Fragments arranged by size:
3079 1614 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6208
HpaI GTT'AAC
Cuts at: 3798 3798
Size: 6208
KasI G'GCGC_C
Cuts at: 2200 2493 2582 2791 3199 2200
Size: 293 89 209 408 5209
Fragments arranged by size:
5209 408 293 209 89
KpnI G_GTAC'C
Cuts at: 3533 3533
Size: 6208
MfeI C'AATT_G
Cuts at: 3807 3807
Size: 6208
MluI A'CGCG_T
Cuts at: 6084 6084
Size: 6208
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2052 5322 5506 1305
Size: 747 3270 184 2007
Fragments arranged by size:
3270 2007 747 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2108 2255 10
Size: 54 1425 343 276 147 3963
Fragments arranged by size:
3963 1425 343 276 147 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2249 2684 4166 4525 4684 518
Size: 648 1083 435 1482 359 159 2042
Fragments arranged by size:
2042 1482 1083 648 435 359 159
MspA1I CmG'CkG
Cuts at: 1606 2198 2789 2905 3257 3291 3432 3439 3624
Size: 592 591 116 352 34 141 7 185
Cuts at: 3624 4301 5449 5694 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 592 591 352 245 185
141 116 34 7
NaeI GCC'GGC
Cuts at: 2498 2752 3039 3133 3216 2498
Size: 254 287 94 83 5490
Fragments arranged by size:
5490 287 254 94 83
NarI GG'CG_CC
Cuts at: 2201 2494 2583 2792 3200 2201
Size: 293 89 209 408 5209
Fragments arranged by size:
5209 408 293 209 89
NcoI C'CATG_G
Cuts at: 513 1065 2115 2250 513
Size: 552 1050 135 4471
Fragments arranged by size:
4471 1050 552 135
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6208
NgoMIV G'CCGG_C
Cuts at: 2496 2750 3037 3131 3214 2496
Size: 254 287 94 83 5490
Fragments arranged by size:
5490 287 254 94 83
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6208
NotI GC'GGCC_GC
Cuts at: 3655 3655
Size: 6208
NspI r_CATG'y
Cuts at: 1132 1229 2470 3004 3049 3566 5111 1132
Size: 97 1241 534 45 517 1545 2229
Fragments arranged by size:
2229 1545 1241 534 517 97 45
PasI CC'CwG_GG
Cuts at: 1348 2041 1348
Size: 693 5515
PciI A'CATG_T
Cuts at: 2466 3045 5107 2466
Size: 579 2062 3567
Fragments arranged by size:
3567 2062 579
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3379 1108
Size: 2271 3937
PmeI GTTT'AAAC
Cuts at: 3512 3512
Size: 6208
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4277 4309 5790 5822 4277
Size: 32 1481 32 4663
Fragments arranged by size:
4663 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2209 3295 2209
Size: 1086 5122
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6208
PsiI TTA'TAA
Cuts at: 3778 3778
Size: 6208
PspOMI G'GGCC_C
Cuts at: 2544 2544
Size: 6208
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2282 2314 2282
Size: 32 6176
PstI C_TGCA'G
Cuts at: 830 2718 3560 830
Size: 1888 842 3478
Fragments arranged by size:
3478 1888 842
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6208
PvuII CAG'CTG
Cuts at: 3432 3439 3432
Size: 7 6201
SacII CC_GC'GG
Cuts at: 2199 2790 3258 2199
Size: 591 468 5149
Fragments arranged by size:
5149 591 468
SalI G'TCGA_C
Cuts at: 1936 3550 1936
Size: 1614 4594
SbfI CC_TGCA'GG
Cuts at: 3560 3560
Size: 6208
ScaI AGT'ACT
Cuts at: 4456 6168 4456
Size: 1712 4496
SexAI A'CCwGG_T
Cuts at: 2211 2211
Size: 6208
SfcI C'TryA_G
Cuts at: 826 1045 2227 2714 3445 3556 4691 5372 5563
Size: 219 1182 487 731 111 1135 681 191
Cuts at: 5563 826
Size: 1471
Fragments arranged by size:
1471 1182 1135 731 681 487 219 191
111
SfoI GGC'GCC
Cuts at: 2202 2495 2584 2793 3201 2202
Size: 293 89 209 408 5209
Fragments arranged by size:
5209 408 293 209 89
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6208
SgrAI Cr'CCGG_yG
Cuts at: 1700 2496 1700
Size: 796 5412
SmaI CCC'GGG
Cuts at: 1872 2871 3247 3535 1872
Size: 999 376 288 4545
Fragments arranged by size:
4545 999 376 288
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2552 4312 5213 5475 820
Size: 197 926 609 1760 901 262 1553
Fragments arranged by size:
1760 1553 926 901 609 262 197
SnaBI TAC'GTA
Cuts at: 493 3999 493
Size: 3506 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6208
SphI G_CATG'C
Cuts at: 1132 3004 3566 1132
Size: 1872 562 3774
Fragments arranged by size:
3774 1872 562
SspI AAT'ATT
Cuts at: 5 52 4132 5
Size: 47 4080 2081
Fragments arranged by size:
4080 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6208
StyI C'CwwG_G
Cuts at: 513 1065 2115 2250 2526 2577 2616 2682 3647
Size: 552 1050 135 276 51 39 66 965
Cuts at: 3647 513
Size: 3074
Fragments arranged by size:
3074 1050 965 552 276 135 66 51
39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4235 4252 4405 4590 5035 325
Size: 1255 2655 17 153 185 445 1498
Fragments arranged by size:
2655 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2145 3018 4454
Size: 274 80 33 51 1443 168 873 1436
Cuts at: 4454 6166 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1436 873 274 168 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6168 6200 6168
Size: 32 6176
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6208
XbaI T'CTAG_A
Cuts at: 3544 3544
Size: 6208
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6208
XmaI C'CCGG_G
Cuts at: 1870 2869 3245 3533 1870
Size: 999 376 288 4545
Fragments arranged by size:
4545 999 376 288
XmnI GAAnn'nnTTC
Cuts at: 4337 4337
Size: 6208
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4016 276
Size: 53 83 186 936 2482 2468
Fragments arranged by size:
2482 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspOMI PsrI PstI PvuI PvuII SacII SalI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbvCI BfrBI BsiWI
BstZ17I DraIII EcoICRI EcoRV FseI FspAI NruI NsiI
PacI PfoI PmlI PspXI RsrII SacI SanDI SapI
SfiI SrfI SwaI XcmI