Restriction map for
pFN21AB9592
(Circular) (Six-base) MAPSORT of: pFN21AB9592.seq Check: 10 from: 1 to: 6484
pFN21AB9592 6484 bp
With 182 enzymes: SgfI *
April 15, 2010 09:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4294 278
Size: 53 83 186 936 2758 2468
Fragments arranged by size:
2758 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3393 3553 3827 1937
Size: 1456 160 274 4594
Fragments arranged by size:
4594 1456 274 160
Acc65I G'GTAC_C
Cuts at: 3805 3805
Size: 6484
AclI AA'CG_TT
Cuts at: 1563 4611 4984 1563
Size: 3048 373 3063
Fragments arranged by size:
3063 3048 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2987 3801 4547 5931 6199 1632
Size: 1355 814 746 1384 268 1917
Fragments arranged by size:
1917 1384 1355 814 746 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6287
AflIII A'CryG_T
Cuts at: 1203 3556 3751 5383 6360 1203
Size: 2353 195 1632 977 1327
Fragments arranged by size:
2353 1632 1327 977 195
AgeI A'CCGG_T
Cuts at: 2664 5315 2664
Size: 2651 3833
AhdI GACnn_n'nnGTC
Cuts at: 3585 5213 3585
Size: 1628 4856
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6484
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2202 1184
Size: 1018 5466
AlwNI CAG_nnn'CTG
Cuts at: 2111 3325 3602 4227 5799 2111
Size: 1214 277 625 1572 2796
Fragments arranged by size:
2796 1572 1214 625 277
ApaLI G'TGCA_C
Cuts at: 4541 5697 4541
Size: 1156 5328
ApoI r'AATT_y
Cuts at: 1493 2331 2436 2540 2710 2729 2780 2816 3793
Size: 838 105 104 170 19 51 36 977
Cuts at: 3793 4019 4171 6280 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 977 838 226 170 152 105
104 51 36 19
AseI AT'TA_AT
Cuts at: 160 1032 5038 160
Size: 872 4006 1606
Fragments arranged by size:
4006 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6484
AvaI C'yCGr_G
Cuts at: 1870 1943 3809 1870
Size: 73 1866 4545
Fragments arranged by size:
4545 1866 73
BamHI G'GATC_C
Cuts at: 2716 3814 4181 2716
Size: 1098 367 5019
Fragments arranged by size:
5019 1098 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2903 3805 5260 618
Size: 325 217 627 1116 902 1455 1842
Fragments arranged by size:
1842 1455 1116 902 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3803 1391
Size: 2412 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3622 928
Size: 971 1723 3790
Fragments arranged by size:
3790 1723 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4675 4709 1903
Size: 34 50 34 2654 34 3678
Fragments arranged by size:
3678 2654 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3296 4376 5586 3296
Size: 1080 1210 4194
Fragments arranged by size:
4194 1210 1080
BclI T'GATC_A
Cuts at: 1548 3360 1548
Size: 1812 4672
BfrBI ATG'CAT
Cuts at: 2775 2775
Size: 6484
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5095 136
Size: 107 122 71 631 605 3423 1525
Fragments arranged by size:
3423 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6479 6479
Size: 6484
BlpI GC'TnA_GC
Cuts at: 3901 3901
Size: 6484
Bme1580I G_kGCm'C
Cuts at: 1790 4545 5701 1790
Size: 2755 1156 2573
Fragments arranged by size:
2755 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 2660 3557 1318
Size: 1342 897 4245
Fragments arranged by size:
4245 1342 897
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4215 5173 442
Size: 416 53 83 399 24 2798 958 1753
Fragments arranged by size:
2798 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6484
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2242 2242
Size: 6484
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3563 3579 3596 5144 1239
Size: 2324 16 17 1548 2579
Fragments arranged by size:
2579 2324 1548 17 16
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4609 5474 5772 4609
Size: 865 298 5321
Fragments arranged by size:
5321 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3572 5147 882
Size: 617 2073 1575 2219
Fragments arranged by size:
2219 2073 1575 617
BsaAI yAC'GTr
Cuts at: 493 1204 4275 493
Size: 711 3071 2702
Fragments arranged by size:
3071 2702 711
BsaBI GATnn'nnATC
Cuts at: 3420 6478 3420
Size: 3058 3426
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4291 4673 5308 275
Size: 53 83 186 936 2758 382 635 1451
Fragments arranged by size:
2758 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2664 4917 5315 5589 5736 6147 2664
Size: 2253 398 274 147 411 3001
Fragments arranged by size:
3001 2253 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6259
Fragments arranged by size:
6259 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2329 1186
Size: 1143 5341
BseYI C'CCAG_C
Cuts at: 5687 6183 5687
Size: 496 5988
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2312 1163
Size: 573 576 5335
Fragments arranged by size:
5335 576 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3934 4695 5723 1540
Size: 275 186 1933 761 1028 2301
Fragments arranged by size:
2301 1933 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4545 4630 5701 4545
Size: 85 1071 5328
Fragments arranged by size:
5328 1071 85
BsmI GAATG_Cn'
Cuts at: 3995 4088 3995
Size: 93 6391
BsmBI CGTCTCn'nnnn_
Cuts at: 2650 2650
Size: 6484
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3803 4545 4630 5701 1391
Size: 399 2013 742 85 1071 2174
Fragments arranged by size:
2174 2013 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 6147 6147
Size: 6484
BspHI T'CATG_A
Cuts at: 4373 6103 4373
Size: 1730 4754
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2369 3839 844
Size: 1525 1470 3489
Fragments arranged by size:
3489 1525 1470
BsrBI CCG'CTC
Cuts at: 1394 1823 4371 1394
Size: 429 2548 3507
Fragments arranged by size:
3507 2548 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4979 5153 65
Size: 1183 3731 174 1396
Fragments arranged by size:
3731 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2664 5128 5315 1700
Size: 110 854 2464 187 2869
Fragments arranged by size:
2869 2464 854 187 110
BsrGI T'GTAC_A
Cuts at: 96 2365 2515 96
Size: 2269 150 4065
Fragments arranged by size:
4065 2269 150
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6484
BssSI C'ACGA_G
Cuts at: 4544 5556 4544
Size: 1012 5472
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6484
BstBI TT'CG_AA
Cuts at: 3477 5288 3477
Size: 1811 4673
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6484
BstXI CCAn_nnnn'nTGG
Cuts at: 2914 2914
Size: 6484
BstYI r'GATC_y
Cuts at: 1972 2716 3814 4181 4566 4583 6035 6121 6133
Size: 744 1098 367 385 17 1452 86 12
Cuts at: 6133 6479 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1098 744 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 3436 3583 513
Size: 552 2371 147 3414
Fragments arranged by size:
3414 2371 552 147
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3706 487
Size: 683 56 2480 3265
Fragments arranged by size:
3265 2480 683 56
BtsI GCAGTG_nn'
Cuts at: 4000 4794 4814 4000
Size: 794 20 5670
Fragments arranged by size:
5670 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6484
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6449
DraI TTT'AAA
Cuts at: 3177 3788 3177
Size: 611 5873
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5491 809
Size: 507 4175 1802
Fragments arranged by size:
4175 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3604 3931 4820
Size: 54 1425 50 275 18 1774 327 889
Cuts at: 4820 6449 8
Size: 1629 43
Fragments arranged by size:
1774 1629 1425 889 327 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3931 1537
Size: 275 2119 4090
Fragments arranged by size:
4090 2119 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4414 1353
Size: 3061 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5071 5445 5591 387
Size: 1064 138 3482 374 146 1280
Fragments arranged by size:
3482 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2987 3563 3579 3596 3801 4547 5144
Size: 393 1355 576 16 17 205 746 597
Cuts at: 5144 5931 6199 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1355 787 746 597 576 393 268
205 17 16
EcoRI G'AATT_C
Cuts at: 2540 2816 3793 4171 2540
Size: 276 977 378 4853
Fragments arranged by size:
4853 977 378 276
FspI TGC'GCA
Cuts at: 4990 4990
Size: 6484
HaeII r_GCGC'y
Cuts at: 1678 5631 1678
Size: 3953 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2111 2143 2204 2222 2236
Size: 32 135 32 539 32 61 18 14
Cuts at: 2236 2254 3121 3153 3571 3603 5125 5157 5199
Size: 18 867 32 418 32 1522 32 42
Cuts at: 5199 5231 1373
Size: 32 2626
Fragments arranged by size:
2626 1522 867 539 418 135 61 42
32 32 32 32 32 32 32 18
18 14
HincII GTy'rAC
Cuts at: 669 1657 1938 3554 3828 4074 669
Size: 988 281 1616 274 246 3079
Fragments arranged by size:
3079 1616 988 281 274 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6484
HpaI GTT'AAC
Cuts at: 4074 4074
Size: 6484
KpnI G_GTAC'C
Cuts at: 3809 3809
Size: 6484
MfeI C'AATT_G
Cuts at: 3503 4083 3503
Size: 580 5904
MluI A'CGCG_T
Cuts at: 6360 6360
Size: 6484
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2514 5598 5782 1305
Size: 1209 3084 184 2007
Fragments arranged by size:
3084 2007 1209 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3606 10
Size: 54 1425 343 1774 2888
Fragments arranged by size:
2888 1774 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2417 4442 4801 4960 518
Size: 648 1251 2025 359 159 2042
Fragments arranged by size:
2042 2025 1251 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2186 3900 4577 5725 5970 1606
Size: 580 1714 677 1148 245 2120
Fragments arranged by size:
2120 1714 1148 677 580 245
NcoI C'CATG_G
Cuts at: 513 1065 3583 513
Size: 552 2518 3414
Fragments arranged by size:
3414 2518 552
NdeI CA'TA_TG
Cuts at: 387 3517 387
Size: 3130 3354
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6484
NotI GC'GGCC_GC
Cuts at: 3931 3931
Size: 6484
NsiI A_TGCA'T
Cuts at: 2777 2777
Size: 6484
NspI r_CATG'y
Cuts at: 1132 1229 2696 3755 3842 5387 1132
Size: 97 1467 1059 87 1545 2229
Fragments arranged by size:
2229 1545 1467 1059 97 87
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6484
PciI A'CATG_T
Cuts at: 3751 5383 3751
Size: 1632 4852
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3382 3574 3602 1108
Size: 2274 192 28 3990
Fragments arranged by size:
3990 2274 192 28
PmeI GTTT'AAAC
Cuts at: 3788 3788
Size: 6484
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4553 4585 6066 6098 4553
Size: 32 1481 32 4939
Fragments arranged by size:
4939 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6484
PsiI TTA'TAA
Cuts at: 4054 4054
Size: 6484
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3362 3394 3362
Size: 32 6452
PstI C_TGCA'G
Cuts at: 830 3239 3836 830
Size: 2409 597 3478
Fragments arranged by size:
3478 2409 597
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6484
PvuII CAG'CTG
Cuts at: 2186 2186
Size: 6484
SalI G'TCGA_C
Cuts at: 1936 3552 3826 1936
Size: 1616 274 4594
Fragments arranged by size:
4594 1616 274
SbfI CC_TGCA'GG
Cuts at: 3836 3836
Size: 6484
ScaI AGT'ACT
Cuts at: 2486 4732 6444 2486
Size: 2246 1712 2526
Fragments arranged by size:
2526 2246 1712
SfcI C'TryA_G
Cuts at: 826 1045 2076 2103 3235 3832 4967 5648 5839
Size: 219 1031 27 1132 597 1135 681 191
Cuts at: 5839 826
Size: 1471
Fragments arranged by size:
1471 1135 1132 1031 681 597 219 191
27
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6484
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6484
SmaI CCC'GGG
Cuts at: 1872 3811 1872
Size: 1939 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4588 5489 5751 820
Size: 197 926 2645 901 262 1553
Fragments arranged by size:
2645 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4275 493
Size: 3782 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6484
SphI G_CATG'C
Cuts at: 1132 3842 1132
Size: 2710 3774
SspI AAT'ATT
Cuts at: 5 52 3229 4408 5
Size: 47 3177 1179 2081
Fragments arranged by size:
3177 2081 1179 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6484
StyI C'CwwG_G
Cuts at: 513 1065 3583 3923 513
Size: 552 2518 340 3074
Fragments arranged by size:
3074 2518 552 340
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4511 4528 4681 4866 5311 325
Size: 1255 2931 17 153 185 445 1498
Fragments arranged by size:
2931 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2365 2484 2515
Size: 274 80 33 51 1443 388 119 31
Cuts at: 2515 2635 4730 6442 96
Size: 120 2095 1712 138
Fragments arranged by size:
2095 1712 1443 388 274 138 120 119
80 51 33 31
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6444 6476 6444
Size: 32 6452
Tth111I GACn'n_nGTC
Cuts at: 1316 2462 1316
Size: 1146 5338
XbaI T'CTAG_A
Cuts at: 3820 3820
Size: 6484
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6484
XmaI C'CCGG_G
Cuts at: 1870 3809 1870
Size: 1939 4545
XmnI GAAnn'nnTTC
Cuts at: 2448 4613 2448
Size: 2165 4319
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4292 276
Size: 53 83 186 936 2758 2468
Fragments arranged by size:
2758 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI
PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI