Restriction map for
pFN21AB9588
(Circular) (Six-base) MAPSORT of: pFN21AB9588.seq Check: 1319 from: 1 to: 5404
pFN21AB9588 5404 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3214 278
Size: 53 83 186 936 1678 2468
Fragments arranged by size:
2468 1678 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2747 1937
Size: 810 4594
Acc65I G'GTAC_C
Cuts at: 2725 2725
Size: 5404
AclI AA'CG_TT
Cuts at: 1563 3531 3904 1563
Size: 1968 373 3063
Fragments arranged by size:
3063 1968 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3467 4851 5119 1632
Size: 1835 1384 268 1917
Fragments arranged by size:
1917 1835 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 2339 820
Size: 197 1322 3885
Fragments arranged by size:
3885 1322 197
AflIII A'CryG_T
Cuts at: 1203 2356 4303 5280 1203
Size: 1153 1947 977 1327
Fragments arranged by size:
1947 1327 1153 977
AgeI A'CCGG_T
Cuts at: 4235 4235
Size: 5404
AhdI GACnn_n'nnGTC
Cuts at: 4133 4133
Size: 5404
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5404
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2043 1184
Size: 859 4545
AlwNI CAG_nnn'CTG
Cuts at: 3147 4719 3147
Size: 1572 3832
ApaLI G'TGCA_C
Cuts at: 2028 3461 4617 2028
Size: 1433 1156 2815
Fragments arranged by size:
2815 1433 1156
ApoI r'AATT_y
Cuts at: 1493 2623 2713 2939 3091 5200 1493
Size: 1130 90 226 152 2109 1697
Fragments arranged by size:
2109 1697 1130 226 152 90
AseI AT'TA_AT
Cuts at: 160 1032 2265 3958 160
Size: 872 1233 1693 1606
Fragments arranged by size:
1693 1606 1233 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5404
AvaI C'yCGr_G
Cuts at: 1870 1943 2729 1870
Size: 73 786 4545
Fragments arranged by size:
4545 786 73
BamHI G'GATC_C
Cuts at: 2734 3101 2734
Size: 367 5037
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2725 4180 618
Size: 325 217 627 938 1455 1842
Fragments arranged by size:
1842 1455 938 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2303 2723 1391
Size: 912 420 4072
Fragments arranged by size:
4072 912 420
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4433
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3595 3629 1903
Size: 34 1658 34 3678
Fragments arranged by size:
3678 1658 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3296 4506 3296
Size: 1210 4194
BclI T'GATC_A
Cuts at: 1548 2221 1548
Size: 673 4731
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4015 136
Size: 107 122 71 631 605 2343 1525
Fragments arranged by size:
2343 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2335 5399 2335
Size: 3064 2340
BlpI GC'TnA_GC
Cuts at: 2092 2821 2092
Size: 729 4675
Bme1580I G_kGCm'C
Cuts at: 1790 2032 3465 4621 1790
Size: 242 1433 1156 2573
Fragments arranged by size:
2573 1433 1156 242
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5404
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3135 4093 442
Size: 416 53 83 399 24 1718 958 1753
Fragments arranged by size:
1753 1718 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5404
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4064 1239
Size: 2825 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2428 3529 4394 4692 2428
Size: 1101 865 298 3140
Fragments arranged by size:
3140 1101 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2396 4067 882
Size: 617 897 1671 2219
Fragments arranged by size:
2219 1671 897 617
BsaAI yAC'GTr
Cuts at: 493 1204 2359 3195 493
Size: 711 1155 836 2702
Fragments arranged by size:
2702 1155 836 711
BsaBI GATnn'nnATC
Cuts at: 5398 5398
Size: 5404
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3211 3593 4228 275
Size: 53 83 186 936 1678 382 635 1451
Fragments arranged by size:
1678 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3837 4235 4509 4656 5067 3837
Size: 398 274 147 411 4174
Fragments arranged by size:
4174 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5179
Fragments arranged by size:
5179 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5404
BseYI C'CCAG_C
Cuts at: 4607 5103 4607
Size: 496 4908
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4831
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2854 3615 4643 1540
Size: 275 186 853 761 1028 2301
Fragments arranged by size:
2301 1028 853 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2032 3465 3550 4621 2032
Size: 1433 85 1071 2815
Fragments arranged by size:
2815 1433 1071 85
BsmI GAATG_Cn'
Cuts at: 2915 3008 2915
Size: 93 5311
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2032 2303 2723 3465 3550 4621 1391
Size: 399 242 271 420 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 420 399 271 242 85
BspEI T'CCGG_A
Cuts at: 5067 5067
Size: 5404
BspHI T'CATG_A
Cuts at: 2415 3293 5023 2415
Size: 878 1730 2796
Fragments arranged by size:
2796 1730 878
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2759 844
Size: 1915 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3291 1394
Size: 429 1468 3507
Fragments arranged by size:
3507 1468 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3899 4073 65
Size: 1183 2651 174 1396
Fragments arranged by size:
2651 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4048 4235 1700
Size: 110 2238 187 2869
Fragments arranged by size:
2869 2238 187 110
BsrGI T'GTAC_A
Cuts at: 96 2353 96
Size: 2257 3147
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5404
BssSI C'ACGA_G
Cuts at: 2225 3464 4476 2225
Size: 1239 1012 3153
Fragments arranged by size:
3153 1239 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5404
BstBI TT'CG_AA
Cuts at: 4208 4208
Size: 5404
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5404
BstYI r'GATC_y
Cuts at: 1972 2335 2734 3101 3486 3503 4955 5041 5053
Size: 363 399 367 385 17 1452 86 12
Cuts at: 5053 5399 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 399 385 367 363 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3913
Fragments arranged by size:
3913 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4665
Fragments arranged by size:
4665 683 56
BtsI GCAGTG_nn'
Cuts at: 2920 3714 3734 2920
Size: 794 20 4590
Fragments arranged by size:
4590 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5404
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5369
DraI TTT'AAA
Cuts at: 2708 2708
Size: 5404
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4411 809
Size: 507 3095 1802
Fragments arranged by size:
3095 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2851 3740 5369
Size: 54 1425 50 275 18 1021 889 1629
Cuts at: 5369 8
Size: 43
Fragments arranged by size:
1629 1425 1021 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2851 1537
Size: 275 1039 4090
Fragments arranged by size:
4090 1039 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2316 3334 1353
Size: 963 1018 3423
Fragments arranged by size:
3423 1018 963
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2162 3991 4365 4511 387
Size: 1064 138 573 1829 374 146 1280
Fragments arranged by size:
1829 1280 1064 573 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3467 4064 4851 5119 1239
Size: 393 1835 597 787 268 1524
Fragments arranged by size:
1835 1524 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2713 3091 2713
Size: 378 5026
FspI TGC'GCA
Cuts at: 3910 3910
Size: 5404
HaeII r_GCGC'y
Cuts at: 1678 4551 1678
Size: 2873 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4045 4077 4119 4151 1373
Size: 32 135 32 2473 32 42 32 2626
Fragments arranged by size:
2626 2473 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2438 2748 2994 669
Size: 988 281 500 310 246 3079
Fragments arranged by size:
3079 988 500 310 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5404
HpaI GTT'AAC
Cuts at: 2994 2994
Size: 5404
KpnI G_GTAC'C
Cuts at: 2729 2729
Size: 5404
MfeI C'AATT_G
Cuts at: 2590 3003 2590
Size: 413 4991
MluI A'CGCG_T
Cuts at: 5280 5280
Size: 5404
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4518 4702 1305
Size: 3213 184 2007
Fragments arranged by size:
3213 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3582
Fragments arranged by size:
3582 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3362 3721 3880 518
Size: 648 2196 359 159 2042
Fragments arranged by size:
2196 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2453 2820 3497 4645 4890 1606
Size: 847 367 677 1148 245 2120
Fragments arranged by size:
2120 1148 847 677 367 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3913
Fragments arranged by size:
3913 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5404
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5404
NotI GC'GGCC_GC
Cuts at: 2851 2851
Size: 5404
NspI r_CATG'y
Cuts at: 1132 1229 2762 4307 1132
Size: 97 1533 1545 2229
Fragments arranged by size:
2229 1545 1533 97
PasI CC'CwG_GG
Cuts at: 1348 2303 1348
Size: 955 4449
PciI A'CATG_T
Cuts at: 4303 4303
Size: 5404
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5404
PmeI GTTT'AAAC
Cuts at: 2708 2708
Size: 5404
PmlI CAC'GTG
Cuts at: 2359 2359
Size: 5404
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3473 3505 4986 5018 3473
Size: 32 1481 32 3859
Fragments arranged by size:
3859 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5404
PsiI TTA'TAA
Cuts at: 2372 2974 2372
Size: 602 4802
PstI C_TGCA'G
Cuts at: 830 2756 830
Size: 1926 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5404
PvuII CAG'CTG
Cuts at: 2453 2453
Size: 5404
SalI G'TCGA_C
Cuts at: 1936 2746 1936
Size: 810 4594
SbfI CC_TGCA'GG
Cuts at: 2756 2756
Size: 5404
ScaI AGT'ACT
Cuts at: 3652 5364 3652
Size: 1712 3692
SfcI C'TryA_G
Cuts at: 826 1045 2752 3887 4568 4759 826
Size: 219 1707 1135 681 191 1471
Fragments arranged by size:
1707 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5404
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5404
SmaI CCC'GGG
Cuts at: 1872 2731 1872
Size: 859 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2339 2407 3508 4409 4671 820
Size: 197 926 396 68 1101 901 262 1553
Fragments arranged by size:
1553 1101 926 901 396 262 197 68
SnaBI TAC'GTA
Cuts at: 493 3195 493
Size: 2702 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5404
SphI G_CATG'C
Cuts at: 1132 2762 1132
Size: 1630 3774
SspI AAT'ATT
Cuts at: 5 52 2080 3328 5
Size: 47 2028 1248 2081
Fragments arranged by size:
2081 2028 1248 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5404
StyI C'CwwG_G
Cuts at: 513 1065 2004 2843 513
Size: 552 939 839 3074
Fragments arranged by size:
3074 939 839 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 1997 3431 3448 3601 3786 4231 325
Size: 1255 417 1434 17 153 185 445 1498
Fragments arranged by size:
1498 1434 1255 445 417 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2353 2594 3650
Size: 274 80 33 51 1443 376 241 1056
Cuts at: 3650 5362 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1056 376 274 241 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5364 5396 5364
Size: 32 5372
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5404
XbaI T'CTAG_A
Cuts at: 2740 2740
Size: 5404
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5404
XmaI C'CCGG_G
Cuts at: 1870 2729 1870
Size: 859 4545
XmnI GAAnn'nnTTC
Cuts at: 2079 2202 2493 3533 2079
Size: 123 291 1040 3950
Fragments arranged by size:
3950 1040 291 123
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3212 276
Size: 53 83 186 936 1678 2468
Fragments arranged by size:
2468 1678 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PpuMI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI