Restriction map for
pFN21AB9587
(Circular) (Six-base) MAPSORT of: pFN21AB9587.seq Check: 5168 from: 1 to: 5413
pFN21AB9587 5413 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2293 2293
Size: 5413
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3223 278
Size: 53 83 186 936 1687 2468
Fragments arranged by size:
2468 1687 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2067 2497 2756 1937
Size: 130 430 259 4594
Fragments arranged by size:
4594 430 259 130
Acc65I G'GTAC_C
Cuts at: 2734 2734
Size: 5413
AclI AA'CG_TT
Cuts at: 1563 3540 3913 1563
Size: 1977 373 3063
Fragments arranged by size:
3063 1977 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2042 3476 4860 5128 1632
Size: 410 1434 1384 268 1917
Fragments arranged by size:
1917 1434 1384 410 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5216
AflIII A'CryG_T
Cuts at: 1203 4312 5289 1203
Size: 3109 977 1327
Fragments arranged by size:
3109 1327 977
AgeI A'CCGG_T
Cuts at: 4244 4244
Size: 5413
AhdI GACnn_n'nnGTC
Cuts at: 4142 4142
Size: 5413
AleI CACnn'nnGTG
Cuts at: 1166 2099 1166
Size: 933 4480
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5413
AlwNI CAG_nnn'CTG
Cuts at: 2489 2576 2597 2686 3156 4728 2489
Size: 87 21 89 470 1572 3174
Fragments arranged by size:
3174 1572 470 89 87 21
ApaI G_GGCC'C
Cuts at: 2483 2483
Size: 5413
ApaLI G'TGCA_C
Cuts at: 3470 4626 3470
Size: 1156 4257
ApoI r'AATT_y
Cuts at: 1493 2722 2948 3100 5209 1493
Size: 1229 226 152 2109 1697
Fragments arranged by size:
2109 1697 1229 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3967 160
Size: 872 2935 1606
Fragments arranged by size:
2935 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5413
AvaI C'yCGr_G
Cuts at: 1870 1943 2738 1870
Size: 73 795 4545
Fragments arranged by size:
4545 795 73
BamHI G'GATC_C
Cuts at: 2743 3110 2743
Size: 367 5046
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2110 2199 2248 2462 2566
Size: 325 217 627 323 89 49 214 104
Cuts at: 2566 2734 4189 618
Size: 168 1455 1842
Fragments arranged by size:
1842 1455 627 325 323 217 214 168
104 89 49
BanII G_rGCy'C
Cuts at: 1391 2483 2732 1391
Size: 1092 249 4072
Fragments arranged by size:
4072 1092 249
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4442
BbvCI CC'TCA_GC
Cuts at: 2369 2369
Size: 5413
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3604 3638 1903
Size: 34 50 34 1583 34 3678
Fragments arranged by size:
3678 1583 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3305 4515 3305
Size: 1210 4203
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5413
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2348 4024 136
Size: 107 122 71 631 605 676 1676 1525
Fragments arranged by size:
1676 1525 676 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5408 5408
Size: 5413
BlpI GC'TnA_GC
Cuts at: 2009 2830 2009
Size: 821 4592
Bme1580I G_kGCm'C
Cuts at: 1790 2483 2569 2678 3474 4630 1790
Size: 693 86 109 796 1156 2573
Fragments arranged by size:
2573 1156 796 693 109 86
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5413
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2289 3144 4102
Size: 416 53 83 399 24 872 855 958
Cuts at: 4102 442
Size: 1753
Fragments arranged by size:
1753 958 872 855 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5413
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2412 2412
Size: 5413
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2381 2708 4073 1239
Size: 1142 327 1365 2579
Fragments arranged by size:
2579 1365 1142 327
Bpu10I CC'TnA_GC
Cuts at: 2369 2369
Size: 5413
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2141 2534 3538 4403 4701 2141
Size: 393 1004 865 298 2853
Fragments arranged by size:
2853 1004 865 393 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4076 882
Size: 617 2577 2219
Fragments arranged by size:
2577 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3204 493
Size: 711 2000 2702
Fragments arranged by size:
2702 2000 711
BsaBI GATnn'nnATC
Cuts at: 5407 5407
Size: 5413
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2240 3220 3602 4237
Size: 53 83 186 936 707 980 382 635
Cuts at: 4237 275
Size: 1451
Fragments arranged by size:
1451 980 936 707 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2626 3846 4244 4518 4665 5076 2626
Size: 1220 398 274 147 411 2963
Fragments arranged by size:
2963 1220 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5188
Fragments arranged by size:
5188 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2051 2054 2057 2060 1186
Size: 865 3 3 3 4539
Fragments arranged by size:
4539 865 3 3 3
BseYI C'CCAG_C
Cuts at: 2453 2476 2482 4616 5112 2453
Size: 23 6 2134 496 2754
Fragments arranged by size:
2754 2134 496 23 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4840
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2121 2696 2863 3624 4652 1540
Size: 275 186 120 575 167 761 1028 2301
Fragments arranged by size:
2301 1028 761 575 275 186 167 120
BsiHKAI G_wGCw'C
Cuts at: 2157 3474 3559 4630 2157
Size: 1317 85 1071 2940
Fragments arranged by size:
2940 1317 1071 85
BsmI GAATG_Cn'
Cuts at: 2924 3017 2924
Size: 93 5320
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2157 2483 2569 2678 2732 3474 3559
Size: 399 367 326 86 109 54 742 85
Cuts at: 3559 4630 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 367 326 109 86
85 54
BspEI T'CCGG_A
Cuts at: 2626 5076 2626
Size: 2450 2963
BspHI T'CATG_A
Cuts at: 3302 5032 3302
Size: 1730 3683
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2202 2293 2481 2768 844
Size: 1358 91 188 287 3489
Fragments arranged by size:
3489 1358 287 188 91
BsrBI CCG'CTC
Cuts at: 1394 1823 3300 1394
Size: 429 1477 3507
Fragments arranged by size:
3507 1477 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3908 4082 65
Size: 1183 2660 174 1396
Fragments arranged by size:
2660 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4057 4244 1700
Size: 110 2247 187 2869
Fragments arranged by size:
2869 2247 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5413
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5413
BssSI C'ACGA_G
Cuts at: 3473 4485 3473
Size: 1012 4401
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2206 794
Size: 1412 4001
BstBI TT'CG_AA
Cuts at: 4217 4217
Size: 5413
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5413
BstXI CCAn_nnnn'nTGG
Cuts at: 2359 2359
Size: 5413
BstYI r'GATC_y
Cuts at: 1972 2083 2743 3110 3495 3512 4964 5050 5062
Size: 111 660 367 385 17 1452 86 12
Cuts at: 5062 5408 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 660 385 367 346 111 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2598 2598
Size: 5413
BtgI C'CryG_G
Cuts at: 513 1065 2121 2244 2253 2346 513
Size: 552 1056 123 9 93 3580
Fragments arranged by size:
3580 1056 552 123 93 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4674
Fragments arranged by size:
4674 683 56
BtsI GCAGTG_nn'
Cuts at: 2929 3723 3743 2929
Size: 794 20 4599
Fragments arranged by size:
4599 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5413
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5378
DraI TTT'AAA
Cuts at: 2717 2717
Size: 5413
DraIII CAC_nnn'GTG
Cuts at: 2206 2206
Size: 5413
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4420 809
Size: 507 3104 1802
Fragments arranged by size:
3104 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2089 2118 2349
Size: 54 1425 50 275 18 259 29 231
Cuts at: 2349 2860 3749 5378 8
Size: 511 889 1629 43
Fragments arranged by size:
1629 1425 889 511 275 259 231 54
50 43 29 18
EagI C'GGCC_G
Cuts at: 1537 1812 2118 2860 1537
Size: 275 306 742 4090
Fragments arranged by size:
4090 742 306 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3343 1353
Size: 1990 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4000 4374 4520 387
Size: 1064 138 2411 374 146 1280
Fragments arranged by size:
2411 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2042 2381 2708 3476 4073 4860 5128
Size: 393 410 339 327 768 597 787 268
Cuts at: 5128 1239
Size: 1524
Fragments arranged by size:
1524 787 768 597 410 393 339 327
268
EcoNI CCTnn'n_nnAGG
Cuts at: 2360 2390 2525 2360
Size: 30 135 5248
Fragments arranged by size:
5248 135 30
EcoO109I rG'GnC_Cy
Cuts at: 2365 2383 2518 2581 2594 2365
Size: 18 135 63 13 5184
Fragments arranged by size:
5184 135 63 18 13
EcoRI G'AATT_C
Cuts at: 2722 3100 2722
Size: 378 5035
FspI TGC'GCA
Cuts at: 3919 3919
Size: 5413
HaeII r_GCGC'y
Cuts at: 1678 2453 4560 1678
Size: 775 2107 2531
Fragments arranged by size:
2531 2107 775
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2002 2034 4054 4086 4128
Size: 32 135 32 430 32 2020 32 42
Cuts at: 4128 4160 1373
Size: 32 2626
Fragments arranged by size:
2626 2020 430 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2068 2757 3003 669
Size: 988 281 130 689 246 3079
Fragments arranged by size:
3079 988 689 281 246 130
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5413
HpaI GTT'AAC
Cuts at: 3003 3003
Size: 5413
KpnI G_GTAC'C
Cuts at: 2738 2738
Size: 5413
MfeI C'AATT_G
Cuts at: 3012 3012
Size: 5413
MluI A'CGCG_T
Cuts at: 5289 5289
Size: 5413
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4527 4711 1305
Size: 3222 184 2007
Fragments arranged by size:
3222 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2091 2351 10
Size: 54 1425 343 259 260 3072
Fragments arranged by size:
3072 1425 343 260 259 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2099 2543 2663 3371 3730 3889 518
Size: 648 933 444 120 708 359 159 2042
Fragments arranged by size:
2042 933 708 648 444 359 159 120
MspA1I CmG'CkG
Cuts at: 1606 2099 2255 2829 3506 4654 4899 1606
Size: 493 156 574 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 574 493 245 156
NcoI C'CATG_G
Cuts at: 513 1065 2244 2346 513
Size: 552 1179 102 3580
Fragments arranged by size:
3580 1179 552 102
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5413
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5413
NotI GC'GGCC_GC
Cuts at: 2860 2860
Size: 5413
NspI r_CATG'y
Cuts at: 1132 1229 2771 4316 1132
Size: 97 1542 1545 2229
Fragments arranged by size:
2229 1545 1542 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5413
PciI A'CATG_T
Cuts at: 4312 4312
Size: 5413
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2407 2489 1108
Size: 1299 82 4032
Fragments arranged by size:
4032 1299 82
PfoI T'CCnGG_A
Cuts at: 2358 2358
Size: 5413
PmeI GTTT'AAAC
Cuts at: 2717 2717
Size: 5413
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3482 3514 4995 5027 3482
Size: 32 1481 32 3868
Fragments arranged by size:
3868 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2365 2365
Size: 5413
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5413
PsiI TTA'TAA
Cuts at: 2983 2983
Size: 5413
PspOMI G'GGCC_C
Cuts at: 2479 2479
Size: 5413
PstI C_TGCA'G
Cuts at: 830 2178 2290 2765 830
Size: 1348 112 475 3478
Fragments arranged by size:
3478 1348 475 112
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5413
SacII CC_GC'GG
Cuts at: 2256 2256
Size: 5413
SalI G'TCGA_C
Cuts at: 1936 2066 2755 1936
Size: 130 689 4594
Fragments arranged by size:
4594 689 130
SbfI CC_TGCA'GG
Cuts at: 2765 2765
Size: 5413
ScaI AGT'ACT
Cuts at: 3661 5373 3661
Size: 1712 3701
SexAI A'CCwGG_T
Cuts at: 2202 2202
Size: 5413
SfcI C'TryA_G
Cuts at: 826 1045 2174 2286 2761 3896 4577 4768 826
Size: 219 1129 112 475 1135 681 191 1471
Fragments arranged by size:
1471 1135 1129 681 475 219 191 112
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5413
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5413
SmaI CCC'GGG
Cuts at: 1872 2740 1872
Size: 868 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2156 2513 3517 4418 4680 820
Size: 197 926 213 357 1004 901 262 1553
Fragments arranged by size:
1553 1004 926 901 357 262 213 197
SnaBI TAC'GTA
Cuts at: 493 3204 493
Size: 2711 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5413
SphI G_CATG'C
Cuts at: 1132 2771 1132
Size: 1639 3774
SspI AAT'ATT
Cuts at: 5 52 3337 5
Size: 47 3285 2081
Fragments arranged by size:
3285 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5413
StyI C'CwwG_G
Cuts at: 513 1065 2244 2346 2406 2466 2852 513
Size: 552 1179 102 60 60 386 3074
Fragments arranged by size:
3074 1179 552 386 102 60 60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2450 3440 3457 3610 3795 4240 325
Size: 1255 870 990 17 153 185 445 1498
Fragments arranged by size:
1498 1255 990 870 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3659 5371 96
Size: 274 80 33 51 1443 1682 1712 138
Fragments arranged by size:
1712 1682 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5373 5405 5373
Size: 32 5381
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5413
XbaI T'CTAG_A
Cuts at: 2749 2749
Size: 5413
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5413
XmaI C'CCGG_G
Cuts at: 1870 2738 1870
Size: 868 4545
XmnI GAAnn'nnTTC
Cuts at: 3542 3542
Size: 5413
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3221 276
Size: 53 83 186 936 1687 2468
Fragments arranged by size:
2468 1687 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
SacII SalI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI
BsmBI BstZ17I EcoICRI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI
PsrI PvuII RsrII SacI SanDI SapI SfiI SfoI
SrfI SwaI XcmI