Restriction map for
pFN21AB9581
(Circular) (Six-base) MAPSORT of: pFN21AB9581.seq Check: 6735 from: 1 to: 5767
pFN21AB9581 5767 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2125 2125
Size: 5767
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3577 278
Size: 53 83 186 936 2041 2468
Fragments arranged by size:
2468 2041 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2148 2415 3110 1937
Size: 211 267 695 4594
Fragments arranged by size:
4594 695 267 211
Acc65I G'GTAC_C
Cuts at: 3088 3088
Size: 5767
AclI AA'CG_TT
Cuts at: 1563 3894 4267 1563
Size: 2331 373 3063
Fragments arranged by size:
3063 2331 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2058 3830 5214 5482 1632
Size: 426 1772 1384 268 1917
Fragments arranged by size:
1917 1772 1384 426 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5570
AflIII A'CryG_T
Cuts at: 1203 4666 5643 1203
Size: 3463 977 1327
Fragments arranged by size:
3463 1327 977
AgeI A'CCGG_T
Cuts at: 4598 4598
Size: 5767
AhdI GACnn_n'nnGTC
Cuts at: 4496 4496
Size: 5767
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5767
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2133 1184
Size: 949 4818
AlwNI CAG_nnn'CTG
Cuts at: 2846 3510 5082 2846
Size: 664 1572 3531
Fragments arranged by size:
3531 1572 664
ApaI G_GGCC'C
Cuts at: 2446 2446
Size: 5767
ApaLI G'TGCA_C
Cuts at: 3824 4980 3824
Size: 1156 4611
ApoI r'AATT_y
Cuts at: 1493 2932 3023 3076 3302 3454 5563 1493
Size: 1439 91 53 226 152 2109 1697
Fragments arranged by size:
2109 1697 1439 226 152 91 53
AseI AT'TA_AT
Cuts at: 160 1032 4321 160
Size: 872 3289 1606
Fragments arranged by size:
3289 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5767
AvaI C'yCGr_G
Cuts at: 1870 1943 2289 2360 2810 2951 3092 1870
Size: 73 346 71 450 141 141 4545
Fragments arranged by size:
4545 450 346 141 141 73 71
BamHI G'GATC_C
Cuts at: 3097 3464 3097
Size: 367 5400
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2267 2339 3088 4543 618
Size: 325 217 627 480 72 749 1455 1842
Fragments arranged by size:
1842 1455 749 627 480 325 217 72
BanII G_rGCy'C
Cuts at: 1391 2360 2446 2773 3086 1391
Size: 969 86 327 313 4072
Fragments arranged by size:
4072 969 327 313 86
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4796
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3958 3992 1903
Size: 34 2021 34 3678
Fragments arranged by size:
3678 2021 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3659 4869 3659
Size: 1210 4557
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5767
BfrBI ATG'CAT
Cuts at: 2521 2521
Size: 5767
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2198 2276 4378
Size: 107 122 71 631 605 526 78 2102
Cuts at: 4378 136
Size: 1525
Fragments arranged by size:
2102 1525 631 605 526 122 107 78
71
BglII A'GATC_T
Cuts at: 5762 5762
Size: 5767
BlpI GC'TnA_GC
Cuts at: 2772 3184 2772
Size: 412 5355
Bme1580I G_kGCm'C
Cuts at: 1790 2446 3828 4984 1790
Size: 656 1382 1156 2573
Fragments arranged by size:
2573 1382 1156 656
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5767
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2233 3498 4456
Size: 416 53 83 399 24 816 1265 958
Cuts at: 4456 442
Size: 1753
Fragments arranged by size:
1753 1265 958 816 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2492 1056
Size: 1436 4331
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2670 4427 1239
Size: 1431 1757 2579
Fragments arranged by size:
2579 1757 1431
Bpu10I CC'TnA_GC
Cuts at: 2157 2157
Size: 5767
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3892 4757 5055 3892
Size: 865 298 4604
Fragments arranged by size:
4604 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2049 2594 4430 882
Size: 617 550 545 1836 2219
Fragments arranged by size:
2219 1836 617 550 545
BsaAI yAC'GTr
Cuts at: 493 1204 3558 493
Size: 711 2354 2702
Fragments arranged by size:
2702 2354 711
BsaBI GATnn'nnATC
Cuts at: 5761 5761
Size: 5767
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3574 3956 4591 275
Size: 53 83 186 936 2041 382 635 1451
Fragments arranged by size:
2041 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2783 4200 4598 4872 5019 5430 2783
Size: 1417 398 274 147 411 3120
Fragments arranged by size:
3120 1417 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5542
Fragments arranged by size:
5542 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2341 1186
Size: 1155 4612
BseYI C'CCAG_C
Cuts at: 2073 2263 2422 2657 2765 2839 4970 5466 2073
Size: 190 159 235 108 74 2131 496 2374
Fragments arranged by size:
2374 2131 496 235 190 159 108 74
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2206 2492 2864 2885 2993 1163
Size: 573 470 286 372 21 108 3937
Fragments arranged by size:
3937 573 470 372 286 108 21
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2308 2760 2801 3217 3978 5006
Size: 275 186 307 452 41 416 761 1028
Cuts at: 5006 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 452 416 307 275 186
41
BsiHKAI G_wGCw'C
Cuts at: 3828 3913 4984 3828
Size: 85 1071 4611
Fragments arranged by size:
4611 1071 85
BsmI GAATG_Cn'
Cuts at: 3278 3371 3278
Size: 93 5674
BsmBI CGTCTCn'nnnn_
Cuts at: 2476 2476
Size: 5767
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2360 2446 2773 3086 3828 3913 4984
Size: 399 570 86 327 313 742 85 1071
Cuts at: 4984 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 570 399 327 313 86
85
BspEI T'CCGG_A
Cuts at: 2783 5430 2783
Size: 2647 3120
BspHI T'CATG_A
Cuts at: 3656 5386 3656
Size: 1730 4037
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2125 3122 844
Size: 1281 997 3489
Fragments arranged by size:
3489 1281 997
BsrBI CCG'CTC
Cuts at: 1394 1823 2509 3654 1394
Size: 429 686 1145 3507
Fragments arranged by size:
3507 1145 686 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2560 4262 4436 65
Size: 1183 1312 1702 174 1396
Fragments arranged by size:
1702 1396 1312 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2365 4411 4598 1700
Size: 110 555 2046 187 2869
Fragments arranged by size:
2869 2046 555 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5767
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5767
BssSI C'ACGA_G
Cuts at: 3827 4839 3827
Size: 1012 4755
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2971 794
Size: 2177 3590
BstBI TT'CG_AA
Cuts at: 4571 4571
Size: 5767
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5767
BstXI CCAn_nnnn'nTGG
Cuts at: 2193 2193
Size: 5767
BstYI r'GATC_y
Cuts at: 1972 3097 3464 3849 3866 5318 5404 5416 5762
Size: 1125 367 385 17 1452 86 12 346
Cuts at: 5762 1972
Size: 1977
Fragments arranged by size:
1977 1452 1125 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2724 513
Size: 552 1659 3556
Fragments arranged by size:
3556 1659 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2048 2930 487
Size: 683 56 822 882 3324
Fragments arranged by size:
3324 882 822 683 56
BtsI GCAGTG_nn'
Cuts at: 3283 4077 4097 3283
Size: 794 20 4953
Fragments arranged by size:
4953 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5767
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5732
DraI TTT'AAA
Cuts at: 3071 3071
Size: 5767
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4774 809
Size: 507 3458 1802
Fragments arranged by size:
3458 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2305 2798 3214
Size: 54 1425 50 275 18 475 493 416
Cuts at: 3214 4103 5732 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 493 475 416 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2305 2798 3214 1537
Size: 275 493 493 416 4090
Fragments arranged by size:
4090 493 493 416 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2022 3697 1353
Size: 669 1675 3423
Fragments arranged by size:
3423 1675 669
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4354 4728 4874 387
Size: 1064 138 2765 374 146 1280
Fragments arranged by size:
2765 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2058 2670 3830 4427 5214 5482 1239
Size: 393 426 612 1160 597 787 268 1524
Fragments arranged by size:
1524 1160 787 612 597 426 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2443 2443
Size: 5767
EcoRI G'AATT_C
Cuts at: 3076 3454 3076
Size: 378 5389
FspI TGC'GCA
Cuts at: 2552 4273 2552
Size: 1721 4046
HaeII r_GCGC'y
Cuts at: 1678 2907 4914 1678
Size: 1229 2007 2531
Fragments arranged by size:
2531 2007 1229
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2125 2157 4408 4440 4482
Size: 32 135 32 553 32 2251 32 42
Cuts at: 4482 4514 1373
Size: 32 2626
Fragments arranged by size:
2626 2251 553 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3111 3357 669
Size: 988 281 1173 246 3079
Fragments arranged by size:
3079 1173 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5767
HpaI GTT'AAC
Cuts at: 3357 3357
Size: 5767
KpnI G_GTAC'C
Cuts at: 3092 3092
Size: 5767
MfeI C'AATT_G
Cuts at: 3366 3366
Size: 5767
MluI A'CGCG_T
Cuts at: 5643 5643
Size: 5767
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4881 5065 1305
Size: 3576 184 2007
Fragments arranged by size:
3576 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3945
Fragments arranged by size:
3945 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3045 3725 4084 4243 518
Size: 648 1879 680 359 159 2042
Fragments arranged by size:
2042 1879 680 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2282 2334 2386 2548 2661 2726 2765 2797
Size: 676 52 52 162 113 65 39 32
Cuts at: 2797 2869 3183 3860 5008 5253 1606
Size: 72 314 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 676 314 245 162 113
72 65 52 52 39 32
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5215
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5767
NheI G'CTAG_C
Cuts at: 1052 2488 1052
Size: 1436 4331
NotI GC'GGCC_GC
Cuts at: 3214 3214
Size: 5767
NsiI A_TGCA'T
Cuts at: 2523 2523
Size: 5767
NspI r_CATG'y
Cuts at: 1132 1229 2024 3125 4670 1132
Size: 97 795 1101 1545 2229
Fragments arranged by size:
2229 1545 1101 795 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5767
PciI A'CATG_T
Cuts at: 4666 4666
Size: 5767
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5767
PfoI T'CCnGG_A
Cuts at: 2169 2169
Size: 5767
PmeI GTTT'AAAC
Cuts at: 3071 3071
Size: 5767
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3836 3868 5349 5381 3836
Size: 32 1481 32 4222
Fragments arranged by size:
4222 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5767
PsiI TTA'TAA
Cuts at: 3337 3337
Size: 5767
PspOMI G'GGCC_C
Cuts at: 2442 2442
Size: 5767
PstI C_TGCA'G
Cuts at: 830 2432 2610 3119 830
Size: 1602 178 509 3478
Fragments arranged by size:
3478 1602 509 178
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5767
PvuII CAG'CTG
Cuts at: 2386 2548 2765 2869 2386
Size: 162 217 104 5284
Fragments arranged by size:
5284 217 162 104
SacII CC_GC'GG
Cuts at: 2727 2727
Size: 5767
SalI G'TCGA_C
Cuts at: 1936 3109 1936
Size: 1173 4594
SbfI CC_TGCA'GG
Cuts at: 3119 3119
Size: 5767
ScaI AGT'ACT
Cuts at: 4015 5727 4015
Size: 1712 4055
SfcI C'TryA_G
Cuts at: 826 1045 2428 2606 3115 4250 4931 5122 826
Size: 219 1383 178 509 1135 681 191 1471
Fragments arranged by size:
1471 1383 1135 681 509 219 191 178
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5767
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5767
SmaI CCC'GGG
Cuts at: 1872 2291 2362 2812 2953 3094 1872
Size: 419 71 450 141 141 4545
Fragments arranged by size:
4545 450 419 141 141 71
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3871 4772 5034 820
Size: 197 926 1928 901 262 1553
Fragments arranged by size:
1928 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3558 493
Size: 3065 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5767
SphI G_CATG'C
Cuts at: 1132 2024 3125 1132
Size: 892 1101 3774
Fragments arranged by size:
3774 1101 892
SspI AAT'ATT
Cuts at: 5 52 3691 5
Size: 47 3639 2081
Fragments arranged by size:
3639 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5767
StyI C'CwwG_G
Cuts at: 513 1065 3206 513
Size: 552 2141 3074
Fragments arranged by size:
3074 2141 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2255 2991 3794 3811 3964 4149 4594
Size: 1255 675 736 803 17 153 185 445
Cuts at: 4594 325
Size: 1498
Fragments arranged by size:
1498 1255 803 736 675 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4013 5725 96
Size: 274 80 33 51 1443 2036 1712 138
Fragments arranged by size:
2036 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2372 2404 5727 5759 2372
Size: 32 3323 32 2380
Fragments arranged by size:
3323 2380 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5767
XbaI T'CTAG_A
Cuts at: 3103 3103
Size: 5767
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5767
XmaI C'CCGG_G
Cuts at: 1870 2289 2360 2810 2951 3092 1870
Size: 419 71 450 141 141 4545
Fragments arranged by size:
4545 450 419 141 141 71
XmnI GAAnn'nnTTC
Cuts at: 3896 3896
Size: 5767
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3575 276
Size: 53 83 186 936 2041 2468
Fragments arranged by size:
2468 2041 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BplI
BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PmlI PpuMI PspXI PsrI RsrII SacI SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI