Restriction map for
pFN21AB9578
(Circular) (Six-base) MAPSORT of: pFN21AB9578.seq Check: 1411 from: 1 to: 5416
pFN21AB9578 5416 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3226 278
Size: 53 83 186 936 1690 2468
Fragments arranged by size:
2468 1690 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2759 1937
Size: 822 4594
Acc65I G'GTAC_C
Cuts at: 2737 2737
Size: 5416
AclI AA'CG_TT
Cuts at: 1563 3543 3916 1563
Size: 1980 373 3063
Fragments arranged by size:
3063 1980 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2305 3479 4863 5131 1632
Size: 673 1174 1384 268 1917
Fragments arranged by size:
1917 1384 1174 673 268
AfeI AGC'GCT
Cuts at: 2276 2276
Size: 5416
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5219
AflIII A'CryG_T
Cuts at: 1203 4315 5292 1203
Size: 3112 977 1327
Fragments arranged by size:
3112 1327 977
AgeI A'CCGG_T
Cuts at: 4247 4247
Size: 5416
AhdI GACnn_n'nnGTC
Cuts at: 4145 4145
Size: 5416
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5416
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5416
AlwNI CAG_nnn'CTG
Cuts at: 2524 2527 3159 4731 2524
Size: 3 632 1572 3209
Fragments arranged by size:
3209 1572 632 3
ApaLI G'TGCA_C
Cuts at: 3473 4629 3473
Size: 1156 4260
ApoI r'AATT_y
Cuts at: 1493 2725 2951 3103 5212 1493
Size: 1232 226 152 2109 1697
Fragments arranged by size:
2109 1697 1232 226 152
AscI GG'CGCG_CC
Cuts at: 2369 2369
Size: 5416
AseI AT'TA_AT
Cuts at: 160 1032 3970 160
Size: 872 2938 1606
Fragments arranged by size:
2938 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5416
AvaI C'yCGr_G
Cuts at: 1870 1943 2678 2741 1870
Size: 73 735 63 4545
Fragments arranged by size:
4545 735 73 63
BamHI G'GATC_C
Cuts at: 2746 3113 2746
Size: 367 5049
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2036 2194 2737 4192 618
Size: 325 217 627 249 158 543 1455 1842
Fragments arranged by size:
1842 1455 627 543 325 249 217 158
BanII G_rGCy'C
Cuts at: 1391 2660 2735 1391
Size: 1269 75 4072
Fragments arranged by size:
4072 1269 75
BbeI G_GCGC'C
Cuts at: 2040 2198 2040
Size: 158 5258
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2274 928
Size: 971 375 4070
Fragments arranged by size:
4070 971 375
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3607 3641 1903
Size: 34 1670 34 3678
Fragments arranged by size:
3678 1670 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3308 4518 3308
Size: 1210 4206
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5416
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2120 2138 2366
Size: 107 122 71 631 605 448 18 228
Cuts at: 2366 2561 4027 136
Size: 195 1466 1525
Fragments arranged by size:
1525 1466 631 605 448 228 195 122
107 71 18
BglII A'GATC_T
Cuts at: 5411 5411
Size: 5416
BlpI GC'TnA_GC
Cuts at: 2672 2833 2672
Size: 161 5255
Bme1580I G_kGCm'C
Cuts at: 1790 3477 4633 1790
Size: 1687 1156 2573
Fragments arranged by size:
2573 1687 1156
BmgBI CAC'GTC
Cuts at: 1318 2440 1318
Size: 1122 4294
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3147 4105 442
Size: 416 53 83 399 24 1730 958 1753
Fragments arranged by size:
1753 1730 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5416
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4076 1239
Size: 2837 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3541 4406 4704 3541
Size: 865 298 4253
Fragments arranged by size:
4253 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2200 2638 4079 882
Size: 617 701 438 1441 2219
Fragments arranged by size:
2219 1441 701 617 438
BsaAI yAC'GTr
Cuts at: 493 1204 3207 493
Size: 711 2003 2702
Fragments arranged by size:
2702 2003 711
BsaBI GATnn'nnATC
Cuts at: 5410 5410
Size: 5416
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2037 2195 2568 3223
Size: 53 83 186 936 504 158 373 655
Cuts at: 3223 3605 4240 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 655 635 504 382 373 186
158 83 53
BsaWI w'CCGG_w
Cuts at: 3849 4247 4521 4668 5079 3849
Size: 398 274 147 411 4186
Fragments arranged by size:
4186 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2597 2627 1178
Size: 30 165 30 1194 30 3967
Fragments arranged by size:
3967 1194 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2696 1186
Size: 1510 3906
BseYI C'CCAG_C
Cuts at: 2052 2064 4619 5115 2052
Size: 12 2555 496 2353
Fragments arranged by size:
2555 2353 496 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4843
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2109 2124 2361 2499 2866 3627
Size: 275 186 108 15 237 138 367 761
Cuts at: 3627 4655 1540
Size: 1028 2301
Fragments arranged by size:
2301 1028 761 367 275 237 186 138
108 15
BsiHKAI G_wGCw'C
Cuts at: 2439 3477 3562 4633 2439
Size: 1038 85 1071 3222
Fragments arranged by size:
3222 1071 1038 85
BsmI GAATG_Cn'
Cuts at: 2927 3020 2927
Size: 93 5323
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2439 2660 2735 3477 3562 4633 1391
Size: 399 649 221 75 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 649 399 221 85 75
BspEI T'CCGG_A
Cuts at: 5079 5079
Size: 5416
BspHI T'CATG_A
Cuts at: 3305 5035 3305
Size: 1730 3686
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2771 844
Size: 1927 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2031 2671 3303 1394
Size: 429 208 640 632 3507
Fragments arranged by size:
3507 640 632 429 208
BsrDI GCAATG_nn'
Cuts at: 65 1248 3911 4085 65
Size: 1183 2663 174 1396
Fragments arranged by size:
2663 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2039 2209 2310 2356 2621 2629 4060
Size: 110 229 170 101 46 265 8 1431
Cuts at: 4060 4247 1700
Size: 187 2869
Fragments arranged by size:
2869 1431 265 229 187 170 110 101
46 8
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5416
BssHII G'CGCG_C
Cuts at: 1925 2151 2369 2511 1925
Size: 226 218 142 4830
Fragments arranged by size:
4830 226 218 142
BssSI C'ACGA_G
Cuts at: 3476 4488 3476
Size: 1012 4404
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2524 794
Size: 1730 3686
BstBI TT'CG_AA
Cuts at: 4220 4220
Size: 5416
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5416
BstYI r'GATC_y
Cuts at: 1972 2746 3113 3498 3515 4967 5053 5065 5411
Size: 774 367 385 17 1452 86 12 346
Cuts at: 5411 1972
Size: 1977
Fragments arranged by size:
1977 1452 774 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2230 2230
Size: 5416
BtgI C'CryG_G
Cuts at: 513 1065 2004 2103 2115 2364 2616 513
Size: 552 939 99 12 249 252 3313
Fragments arranged by size:
3313 939 552 252 249 99 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4677
Fragments arranged by size:
4677 683 56
BtsI GCAGTG_nn'
Cuts at: 2483 2932 3726 3746 2483
Size: 449 794 20 4153
Fragments arranged by size:
4153 794 449 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5416
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5381
DraI TTT'AAA
Cuts at: 2720 2720
Size: 5416
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4423 809
Size: 507 3107 1802
Fragments arranged by size:
3107 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2041 2106 2121
Size: 54 1425 50 275 18 211 65 15
Cuts at: 2121 2221 2358 2454 2619 2863 3752 5381 8
Size: 100 137 96 165 244 889 1629 43
Fragments arranged by size:
1629 1425 889 275 244 211 165 137
100 96 65 54 50 43 18 15
EagI C'GGCC_G
Cuts at: 1537 1812 2106 2121 2358 2863 1537
Size: 275 294 15 237 505 4090
Fragments arranged by size:
4090 505 294 275 237 15
EarI CTCTTCn'nnn_
Cuts at: 1353 3346 1353
Size: 1993 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4003 4377 4523 387
Size: 1064 138 2414 374 146 1280
Fragments arranged by size:
2414 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2305 3479 4076 4863 5131 1239
Size: 393 673 1174 597 787 268 1524
Fragments arranged by size:
1524 1174 787 673 597 393 268
EcoRI G'AATT_C
Cuts at: 2725 3103 2725
Size: 378 5038
FspI TGC'GCA
Cuts at: 3922 3922
Size: 5416
HaeII r_GCGC'y
Cuts at: 1678 2040 2198 2255 2278 2553 4563 1678
Size: 362 158 57 23 275 2010 2531
Fragments arranged by size:
2531 2010 362 275 158 57 23
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2597 2629 2642 2674 4057
Size: 32 135 32 1025 32 13 32 1383
Cuts at: 4057 4089 4131 4163 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1383 1025 135 42 32 32 32
32 32 32 13
HincII GTy'rAC
Cuts at: 669 1657 1938 2410 2760 3006 669
Size: 988 281 472 350 246 3079
Fragments arranged by size:
3079 988 472 350 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5416
HpaI GTT'AAC
Cuts at: 3006 3006
Size: 5416
KasI G'GCGC_C
Cuts at: 2036 2194 2036
Size: 158 5258
KpnI G_GTAC'C
Cuts at: 2741 2741
Size: 5416
MfeI C'AATT_G
Cuts at: 3015 3015
Size: 5416
MluI A'CGCG_T
Cuts at: 5292 5292
Size: 5416
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2625 2628 4530 4714 1305
Size: 1320 3 1902 184 2007
Fragments arranged by size:
2007 1902 1320 184 3
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3594
Fragments arranged by size:
3594 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3374 3733 3892 518
Size: 648 2208 359 159 2042
Fragments arranged by size:
2208 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2117 2138 2203 2325 2524 2832 3509 4657
Size: 511 21 65 122 199 308 677 1148
Cuts at: 4657 4902 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 511 308 245 199 122
65 21
NaeI GCC'GGC
Cuts at: 2041 2312 2358 2623 2041
Size: 271 46 265 4834
Fragments arranged by size:
4834 271 265 46
NarI GG'CG_CC
Cuts at: 2037 2195 2037
Size: 158 5258
NcoI C'CATG_G
Cuts at: 513 1065 2004 2616 513
Size: 552 939 612 3313
Fragments arranged by size:
3313 939 612 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5416
NgoMIV G'CCGG_C
Cuts at: 2039 2310 2356 2621 2039
Size: 271 46 265 4834
Fragments arranged by size:
4834 271 265 46
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5416
NotI GC'GGCC_GC
Cuts at: 2121 2863 2121
Size: 742 4674
NspI r_CATG'y
Cuts at: 1132 1229 2774 4319 1132
Size: 97 1545 1545 2229
Fragments arranged by size:
2229 1545 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5416
PciI A'CATG_T
Cuts at: 4315 4315
Size: 5416
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5416
PmeI GTTT'AAAC
Cuts at: 2720 2720
Size: 5416
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3485 3517 4998 5030 3485
Size: 32 1481 32 3871
Fragments arranged by size:
3871 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5416
PsiI TTA'TAA
Cuts at: 2986 2986
Size: 5416
PstI C_TGCA'G
Cuts at: 830 2520 2768 830
Size: 1690 248 3478
Fragments arranged by size:
3478 1690 248
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5416
PvuII CAG'CTG
Cuts at: 2203 2524 2203
Size: 321 5095
SacII CC_GC'GG
Cuts at: 2118 2118
Size: 5416
SalI G'TCGA_C
Cuts at: 1936 2758 1936
Size: 822 4594
SbfI CC_TGCA'GG
Cuts at: 2768 2768
Size: 5416
ScaI AGT'ACT
Cuts at: 3664 5376 3664
Size: 1712 3704
SfcI C'TryA_G
Cuts at: 826 1045 2335 2516 2764 3899 4580 4771 826
Size: 219 1290 181 248 1135 681 191 1471
Fragments arranged by size:
1471 1290 1135 681 248 219 191 181
SfoI GGC'GCC
Cuts at: 2038 2196 2038
Size: 158 5258
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5416
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5416
SmaI CCC'GGG
Cuts at: 1872 2743 1872
Size: 871 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3520 4421 4683 820
Size: 197 926 1577 901 262 1553
Fragments arranged by size:
1577 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3207 493
Size: 2714 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5416
SphI G_CATG'C
Cuts at: 1132 2774 1132
Size: 1642 3774
SspI AAT'ATT
Cuts at: 5 52 3340 5
Size: 47 3288 2081
Fragments arranged by size:
3288 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5416
StyI C'CwwG_G
Cuts at: 513 1065 2004 2616 2855 513
Size: 552 939 612 239 3074
Fragments arranged by size:
3074 939 612 552 239
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3443 3460 3613 3798 4243 325
Size: 1255 1863 17 153 185 445 1498
Fragments arranged by size:
1863 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2502 3662 5374
Size: 274 80 33 51 1443 525 1160 1712
Cuts at: 5374 96
Size: 138
Fragments arranged by size:
1712 1443 1160 525 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5376 5408 5376
Size: 32 5384
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5416
XbaI T'CTAG_A
Cuts at: 2752 2752
Size: 5416
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5416
XmaI C'CCGG_G
Cuts at: 1870 2741 1870
Size: 871 4545
XmnI GAAnn'nnTTC
Cuts at: 3545 3545
Size: 5416
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3224 276
Size: 53 83 186 936 1690 2468
Fragments arranged by size:
2468 1690 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AscI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PstI PvuI PvuII SacII SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspAI NruI NsiI PacI
PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI
SanDI SapI SexAI SfiI SrfI SwaI XcmI